Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,921,064 C→T 5.9% G56G (GGG→GGA ytrB ← ABC transporter ATP‑binding protein YtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,921,0640CT 5.9% 563.7 / 16.5 169G56G (GGG→GGAytrBABC transporter ATP‑binding protein YtrB
Reads supporting (aligned to +/- strand):  ref base C (91/68);  new base T (5/5);  total (96/73)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 169 total aligned reads displayed.

CTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATC  >  NZ_CP034943/2920939‑2921202
                                                                                                                             |                                                                                                                                          
 ttAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGaaa                                                                                                                             <  2:124501/140‑1 (MQ=255)
 ttAGGATATCGACTAGCTGATTGTATCTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGaaa                                                                                                                             <  2:2768432/140‑1 (MQ=255)
   aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                                           >  1:299716/1‑140 (MQ=255)
   aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                                           >  1:2104051/1‑140 (MQ=255)
   aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                                           >  1:2103950/1‑140 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGAGCTTCGGATGCTTTTTGATTTCTCCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                     <  2:3003306/140‑1 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                     <  1:867847/140‑1 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGAATGTAGATGATATTTTGTTTGCCCTTCGGATGCTTTTTGATTTATACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                     <  1:609255/140‑1 (MQ=255)
          cGACAAGCTGCTAGTATGTATACTTGTCGTAAAAAGTATTTTGAACAGGCATGTAGATGATATTTTGTTTGACATTCGGATGATATTTGATTTCTACGCCATCAAACAAAATAGTCCCGCTGTCGGCGAAAAAGAgttcc                                                                                                                    >  2:2521939/1‑138 (MQ=255)
            aCTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTg                                                                                                                  >  1:2537225/1‑140 (MQ=255)
                gctgctTGTTTGTATACTGGTCGTAAAACGGATTTTGAACAGGCATGTCGATGATATTTTGTTTGACCTTCGGAGGCTTTTTGATTTCTACGCCATCAAACACAATCGTCCCGCTGTCGGCGAAACTGAGTTGCTGGAtt                                                                                                              <  2:2430418/140‑1 (MQ=255)
                gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                              >  2:2450207/1‑140 (MQ=255)
                gctgctTGTATGTATACTGGTCGTAAAAAGGATTTAGAACAGGCATGTAGATGATATTTGGTCTGCCCTGCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                              <  1:625464/140‑1 (MQ=255)
                gctgctTGTATGTAACCTTGTCGTAAAAAGGAGTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTGCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                              <  1:2258304/140‑1 (MQ=255)
                     ttGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                                         <  1:1946981/140‑1 (MQ=255)
                     ttGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                                         <  1:795444/140‑1 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  1:1160157/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  1:1454732/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  1:2414467/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  2:132117/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  1:539818/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                        >  1:41932/1‑140 (MQ=255)
                            tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                  <  2:660155/140‑1 (MQ=255)
                            tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                  <  1:1297766/140‑1 (MQ=255)
                             ataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTg                                                                                                 <  2:2657905/140‑1 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTc                                                                                                                                    >  1:1075306/1‑104 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTc                                                                                                                                    <  2:1075306/104‑1 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                >  1:1047022/1‑140 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                >  2:2912789/1‑140 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                >  1:992087/1‑140 (MQ=255)
                                 ttGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGt                                                                                             <  2:2794453/140‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAt                                                                                                                        >  1:1200547/1‑112 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            <  1:3059692/140‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            >  1:2632716/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            >  1:2696554/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            >  2:1375419/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            >  2:1701694/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            >  1:1241138/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                            <  1:1376089/140‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGtt                                                                                            >  1:163675/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATCTTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCgatg                                                                                           >  1:1022330/1‑137 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCtttt                                                                                           >  1:2904814/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                           >  1:781276/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                           >  2:2495538/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                           >  2:1399984/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                           >  1:2933435/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGttt                                                                                           >  1:856378/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATGTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTCAGCGAAGCATTGTCGtgt                                                                                           >  1:2990140/1‑138 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                          >  2:2396850/1‑140 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                          >  2:1777305/1‑140 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                          >  2:777740/1‑140 (MQ=255)
                                       tAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGcc                                                                                       >  2:2622912/1‑140 (MQ=255)
                                         aaaaGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                        >  1:1545911/1‑137 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGCCCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  1:964257/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  2:1891234/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  1:2914105/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  2:2577017/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAGTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  2:509228/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATAGTGCTGGACCTGCGTATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTGGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                     <  1:1618216/140‑1 (MQ=255)
                                                          gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                                    >  2:2669957/1‑140 (MQ=255)
                                                          gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                                    >  2:1572287/1‑140 (MQ=255)
                                                                aGATGATTTTTTGTTTGACCTTCGGATGATTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                              <  2:643138/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                              <  2:645916/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                              <  2:1947379/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                              <  2:1161428/140‑1 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTCCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCACTTGCGGCCGAGCAGCCCGAAGa                                                          >  1:1700312/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTTCGGCCGAGCCGCCCGAAGa                                                          >  2:1528664/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                          >  1:2037131/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                          >  2:916274/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAGTTGCTGGATTAATCGAAGAATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCcgacga                                                          >  2:1638479/1‑140 (MQ=255)
                                                                     atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                         >  2:952221/1‑140 (MQ=255)
                                                                     atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                         >  1:2618560/1‑140 (MQ=255)
                                                                             tttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtt                                                 >  1:1042458/1‑140 (MQ=255)
                                                                               tgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtttt                                               >  2:332766/1‑140 (MQ=255)
                                                                               tgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtttt                                               >  2:1138849/1‑140 (MQ=255)
                                                                                gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                                              <  2:2261615/140‑1 (MQ=255)
                                                                                  ccTTCGGATGCTTTTTGATTTCTACGGCATCAAACAAAATCGTCGCGCTGTCGGCGAATATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                            <  2:2501669/140‑1 (MQ=255)
                                                                                  ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                            <  2:1467568/140‑1 (MQ=255)
                                                                                  ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                            <  2:1467567/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                   <  2:1120100/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                   <  2:834799/140‑1 (MQ=255)
                                                                                             tttttGATTTCTACGCCATCAAACACAATCGTCCCGCTGTCGGCTAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGa                                 <  2:535496/140‑1 (MQ=255)
                                                                                             tttttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATGTCTCCTTTTTCAATTGTTAATGa                                 >  2:2821178/1‑140 (MQ=255)
                                                                                               tttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGaaa                               >  2:1238038/1‑140 (MQ=255)
                                                                                               tttGATTTCTACGACATCAAACAAAATCGTCCCGCTGTCTGAGACAATGATTTGCTGTATTAAGCGAAGCATTGTCGTTTTGCATGACCCATTGCGGCCAAGCAGCACGAATATATCTCCTTTTTCAAGTAATAATGaaa                               >  2:665768/1‑140 (MQ=255)
                                                                                                ttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAAc                              >  2:657830/1‑140 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                  >  1:861535/1‑65 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                  <  2:861535/65‑1 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAGTTCTCCTTTTTCAATTGTTAATGAAACATCtttt                       >  2:1346170/1‑140 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                 <  1:607016/138‑1 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                 <  1:777740/138‑1 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                 <  1:1777305/138‑1 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                 <  1:2610478/138‑1 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                 <  1:2396850/138‑1 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTTCCTGACCCATTGCGGCCGAGCAGACCGAAGATTTCTCCTTTTTCAATTGTTAATGAAAAATCTTTTATTAATTGATTTCAGt       >  1:876303/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt       >  1:1009925/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt       >  2:1209043/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt       >  2:2558716/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt       >  1:1896516/1‑140 (MQ=255)
                                                                                                                            tCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATc  >  1:475861/1‑140 (MQ=255)
                                                                                                                             |                                                                                                                                          
CTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATC  >  NZ_CP034943/2920939‑2921202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: