Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,921,073 G→A 5.8% A53A (GCC→GCT ytrB ← ABC transporter ATP‑binding protein YtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,921,0730GA 5.8% 567.8 / 16.6 173A53A (GCC→GCTytrBABC transporter ATP‑binding protein YtrB
Reads supporting (aligned to +/- strand):  ref base G (91/71);  new base A (5/5);  total (97/76)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

Only 100 of 174 total aligned reads displayed.

CTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACA  >  NZ_CP034943/2920939‑2921212
                                                                                                                                      |                                                                                                                                           
   aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                                                     >  1:299716/1‑140 (MQ=255)
   aGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAAt                                                                                                                                     >  1:2104051/1‑140 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                               <  1:867847/140‑1 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                               <  1:2685591/140‑1 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                               <  2:1296581/140‑1 (MQ=255)
         tCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGAATGTAGATGATATTTTGTTTGCCCTTCGGATGCTTTTTGATTTATACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTg                                                                                                                               <  1:609255/140‑1 (MQ=255)
          cGACAAGCTGCTAGTATGTATACTTGTCGTAAAAAGTATTTTGAACAGGCATGTAGATGATATTTTGTTTGACATTCGGATGATATTTGATTTCTACGCCATCAAACAAAATAGTCCCGCTGTCGGCGAAAAAGAgttcc                                                                                                                              >  2:2521939/1‑138 (MQ=255)
            aCTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTg                                                                                                                            >  1:2537225/1‑140 (MQ=255)
                gctgctTGTTTGTATACTGGTCGTAAAACGGATTTTGAACAGGCATGTCGATGATATTTTGTTTGACCTTCGGAGGCTTTTTGATTTCTACGCCATCAAACACAATCGTCCCGCTGTCGGCGAAACTGAGTTGCTGGAtt                                                                                                                        <  2:2430418/140‑1 (MQ=255)
                gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                                        >  2:1118768/1‑140 (MQ=255)
                gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                                        <  1:2538394/140‑1 (MQ=255)
                gctgctTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                                        >  2:2450207/1‑140 (MQ=255)
                gctgctTGTATGTATACTGGTCGTAAAAAGGATTTAGAACAGGCATGTAGATGATATTTGGTCTGCCCTGCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAtt                                                                                                                        <  1:625464/140‑1 (MQ=255)
                     ttGTATGTATACTTGCCGTAAAAAGGATTGTGAACAGGCATGTAGATGATATTTTGTTTGCCCTGCGGATGCTTTTTGAGTTCTACTCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCg                                                                                                                   <  1:795948/140‑1 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                                  >  1:2414467/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                                  >  1:520853/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                                  >  1:41932/1‑140 (MQ=255)
                      tgtatgtaTACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGa                                                                                                                  >  1:1454732/1‑140 (MQ=255)
                          tgtatACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCa                                                                                                              <  1:1119211/140‑1 (MQ=255)
                            tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                            <  1:1297766/140‑1 (MQ=255)
                             ataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTg                                                                                                           <  2:2657905/140‑1 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                          >  1:992087/1‑140 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                          >  1:1806449/1‑140 (MQ=255)
                              taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                          >  1:1047022/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAt                                                                                                                                  <  2:1200547/112‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  1:2632716/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      <  1:3059692/140‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  1:551655/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  1:1178813/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  1:1241138/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  2:1701694/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      <  2:1241138/140‑1 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                      >  1:871937/1‑140 (MQ=255)
                                  tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGtt                                                                                                      >  1:163675/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATCTTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCgatg                                                                                                     >  1:1022330/1‑137 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  1:2933435/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  2:2830496/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  1:781276/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  1:785121/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  1:856400/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                     >  2:2054048/1‑140 (MQ=255)
                                   gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTAAGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTTCTGGATTAAGCGAAGCATTGTCtttt                                                                                                     >  1:2763472/1‑140 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                    >  2:2396850/1‑140 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                    >  2:777740/1‑140 (MQ=255)
                                    tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                    >  2:607016/1‑140 (MQ=255)
                                      gTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                                  <  2:1521124/140‑1 (MQ=255)
                                       tAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGcc                                                                                                 >  2:2622912/1‑140 (MQ=255)
                                         aaaaGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                                  >  1:1545911/1‑137 (MQ=255)
                                         aaaaGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                                  <  2:1545911/137‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  1:2088567/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  1:1952892/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  1:2914105/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  2:2577017/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  2:1891234/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGAGTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  2:509228/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAGTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCGTCCCCCATTGCGGCCGag                                                                               >  2:2529189/1‑140 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTCTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  2:1770537/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATTTTGTTTGACATTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  1:1344925/140‑1 (MQ=255)
                                                         ggCATGTAGATGATATAGTGCTGGACCTGCGTATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTGGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                               <  1:1618216/140‑1 (MQ=255)
                                                          gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTTCCTGACCCATTGCGGCCGagc                                                                              >  2:1412304/1‑140 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                        <  1:2230003/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                        <  2:1947379/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                        <  2:514916/140‑1 (MQ=255)
                                                                aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                        <  2:645916/140‑1 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTCCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCACTTGCGGCCGAGCAGCCCGAAGa                                                                    >  1:1700312/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                                    >  2:916274/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                                    >  1:2939254/1‑140 (MQ=255)
                                                                    gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAGTTGCTGGATTAATCGAAGAATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCcgacga                                                                    >  2:1638479/1‑140 (MQ=255)
                                                                     atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                                   >  2:952221/1‑140 (MQ=255)
                                                                     atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                                   >  1:2618560/1‑140 (MQ=255)
                                                                         tttgtttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTtct                                                               >  2:2478060/1‑140 (MQ=255)
                                                                           tgtttgACCTTCGGATGCTATTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTcc                                                             <  2:1343260/140‑1 (MQ=255)
                                                                             tttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtt                                                           >  1:1042458/1‑140 (MQ=255)
                                                                                gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                                                        <  2:2261615/140‑1 (MQ=255)
                                                                                  ccTTCGGATGCTTTTTGATTTCTACGGCATCAAACAAAATCGTCGCGCTGTCGGCGAATATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                                      <  2:2501669/140‑1 (MQ=255)
                                                                                  ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                                      <  2:1467568/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                             <  2:1120100/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                             <  2:658804/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                             <  2:3079729/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                             <  2:834799/140‑1 (MQ=255)
                                                                                           gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                             <  2:1150338/140‑1 (MQ=255)
                                                                                            cTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATg                                            <  2:2734986/140‑1 (MQ=255)
                                                                                            cTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATg                                            >  1:2310234/1‑140 (MQ=255)
                                                                                             tttttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGa                                           >  2:1390766/1‑140 (MQ=255)
                                                                                             tttttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATGTCTCCTTTTTCAATTGTTAATGa                                           >  2:2821178/1‑140 (MQ=255)
                                                                                               tttGATTTCTACGACATCAAACAAAATCGTCCCGCTGTCTGAGACAATGATTTGCTGTATTAAGCGAAGCATTGTCGTTTTGCATGACCCATTGCGGCCAAGCAGCACGAATATATCTCCTTTTTCAAGTAATAATGaaa                                         >  2:665768/1‑140 (MQ=255)
                                                                                                ttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAAc                                        >  2:657830/1‑140 (MQ=255)
                                                                                                      tCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTCATGAAACATCttt                                  >  2:2996590/1‑140 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                            <  2:861535/65‑1 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCtttt                                 >  2:2769300/1‑140 (MQ=255)
                                                                                                       cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAGTTCTCCTTTTTCAATTGTTAATGAAACATCtttt                                 >  2:1346170/1‑140 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                           <  1:607016/138‑1 (MQ=255)
                                                                                                             cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                           <  1:1777305/138‑1 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                 >  2:1209043/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                 >  1:1009925/1‑140 (MQ=255)
                                                                                                                       aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                 >  1:1896516/1‑140 (MQ=255)
                                                                                                                            tCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATc            >  1:940947/1‑140 (MQ=255)
                                                                                                                                 cTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGtttt       >  2:265603/1‑140 (MQ=255)
                                                                                                                                     cGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGAc   <  1:731278/140‑1 (MQ=255)
                                                                                                                                     cGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGAc   <  1:400956/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                           
CTTAGGATATCGACTAGCTGCTTGTATGTATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACA  >  NZ_CP034943/2920939‑2921212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: