Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,921,106 T→C 6.4% T42T (ACA→ACG ytrB ← ABC transporter ATP‑binding protein YtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,921,1060TC 6.4% 514.7 / 17.5 157T42T (ACA→ACGytrBABC transporter ATP‑binding protein YtrB
Reads supporting (aligned to +/- strand):  ref base T (81/66);  new base C (5/5);  total (86/71)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 161 total aligned reads displayed.

TATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGCCT  >  NZ_CP034943/2920967‑2921242
                                                                                                                                           |                                                                                                                                        
tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                                                          <  2:660155/140‑1 (MQ=255)
tataCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAtt                                                                                                                                          <  1:1297766/140‑1 (MQ=255)
  taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                                                        >  1:1047022/1‑140 (MQ=255)
  taCTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGt                                                                                                                                        >  1:1806449/1‑140 (MQ=255)
     ttGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGt                                                                                                                                     <  2:2794453/140‑1 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    <  2:2651873/140‑1 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    >  2:1701694/1‑140 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    >  2:1375419/1‑140 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    <  1:1376089/140‑1 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    >  1:871937/1‑140 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGtt                                                                                                                                    <  1:3059692/140‑1 (MQ=255)
      tGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGtt                                                                                                                                    >  1:163675/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATCTTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCgatg                                                                                                                                   >  1:1022330/1‑137 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  2:2495538/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  1:785121/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  2:1399984/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  1:856400/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  2:2830496/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  2:2054048/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGttt                                                                                                                                   >  1:3017390/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCAGTGTCGttt                                                                                                                                   >  1:856378/1‑140 (MQ=255)
       gTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTAAGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTTCTGGATTAAGCGAAGCATTGTCtttt                                                                                                                                   >  1:2763472/1‑140 (MQ=255)
        tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                                                  >  2:777740/1‑140 (MQ=255)
        tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                                                  >  2:1777305/1‑140 (MQ=255)
        tCGTAAAACGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACTTTCGGGTGCTTTTTGATTTCTACGCCGTCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGtttt                                                                                                                                  >  2:2610478/1‑140 (MQ=255)
          gTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                                                                <  2:950974/140‑1 (MQ=255)
             aaaaGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGc                                                                                                                                >  1:1545911/1‑137 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGCCCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                                                             <  1:964257/140‑1 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                                                             <  2:1891234/140‑1 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                                                             <  1:2914105/140‑1 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                                                             <  1:1952892/140‑1 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAGTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCGTCCCCCATTGCGGCCGag                                                                                                             >  2:2529189/1‑140 (MQ=255)
                             ggCATGTAGATGATATTTTGTTTGACATTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGag                                                                                                             <  1:1344925/140‑1 (MQ=255)
                              gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTTCCTGACCCATTGCGGCCGagc                                                                                                            >  2:1412304/1‑140 (MQ=255)
                              gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                                                                            >  2:2669957/1‑140 (MQ=255)
                              gCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGagc                                                                                                            >  2:1572287/1‑140 (MQ=255)
                                    aGATGATTTTTTGTTTGACCTTCGGATGATTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                                                      <  2:643138/140‑1 (MQ=255)
                                    aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGGAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                                                      <  2:90264/140‑1 (MQ=255)
                                    aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                                                      <  2:514916/140‑1 (MQ=255)
                                    aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                                                      <  2:645916/140‑1 (MQ=255)
                                    aGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCg                                                                                                      <  1:2230003/140‑1 (MQ=255)
                                        gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTCCGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCACTTGCGGCCGAGCAGCCCGAAGa                                                                                                  >  1:1700312/1‑140 (MQ=255)
                                        gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAACTGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTTCGGCCGAGCCGCCCGAAGa                                                                                                  >  2:1528664/1‑140 (MQ=255)
                                        gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                                                                  >  1:2939254/1‑140 (MQ=255)
                                        gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGa                                                                                                  >  1:2037131/1‑140 (MQ=255)
                                        gatATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGAGTTGCTGGATTAATCGAAGAATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCcgacga                                                                                                  >  2:1638479/1‑140 (MQ=255)
                                         atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                                                                 >  2:952221/1‑140 (MQ=255)
                                         atatTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAt                                                                                                 >  1:2618560/1‑140 (MQ=255)
                                             tttgtttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTtct                                                                                             >  2:2478060/1‑140 (MQ=255)
                                               tgtttgACCTTCGGATGCTATTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTcc                                                                                           <  2:1343260/140‑1 (MQ=255)
                                                 tttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtt                                                                                         >  1:1042770/1‑140 (MQ=255)
                                                  ttgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttt                                                                                        <  2:2408797/140‑1 (MQ=255)
                                                   tgACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCtttt                                                                                       >  2:332766/1‑140 (MQ=255)
                                                    gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                                                                                      <  2:1039370/140‑1 (MQ=255)
                                                    gACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCttttt                                                                                      <  2:2261615/140‑1 (MQ=255)
                                                      ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                                                                    <  2:1467567/140‑1 (MQ=255)
                                                      ccTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCa                                                                                    <  2:1467568/140‑1 (MQ=255)
                                                       cTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCaa                                                                                   <  2:2515553/140‑1 (MQ=255)
                                                               gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                                                           <  2:3079729/140‑1 (MQ=255)
                                                               gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                                                           <  2:834799/140‑1 (MQ=255)
                                                               gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                                                           <  2:1120100/140‑1 (MQ=255)
                                                               gCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAAt                                                                           <  2:1150338/140‑1 (MQ=255)
                                                                 tttttGATTTCTACGCCATCAAACACAATCGTCCCGCTGTCGGCTAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGa                                                                         <  2:535496/140‑1 (MQ=255)
                                                                   tttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGaaa                                                                       >  2:1238038/1‑140 (MQ=255)
                                                                   tttGATTTCTACGACATCAAACAAAATCGTCCCGCTGTCTGAGACAATGATTTGCTGTATTAAGCGAAGCATTGTCGTTTTGCATGACCCATTGCGGCCAAGCAGCACGAATATATCTCCTTTTTCAAGTAATAATGaaa                                                                       >  2:665768/1‑140 (MQ=255)
                                                                    ttGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAAc                                                                      >  2:657830/1‑140 (MQ=255)
                                                                          tCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTCATGAAACATCttt                                                                >  2:2996590/1‑140 (MQ=255)
                                                                           cTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCtttt                                                               >  2:2769300/1‑140 (MQ=255)
                                                                                 cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                                                         <  1:1777305/138‑1 (MQ=255)
                                                                                 cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                                                         <  1:777740/138‑1 (MQ=255)
                                                                                 cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                                                         <  1:607016/138‑1 (MQ=255)
                                                                                 cgtCAAATAAAATCGTTCCGCTGTCAGCGAAAATGATTTGCTGGATTAAGCGAAGCATCGTCGTTTTGCCCGATCCATTGCGTCCAAGCAATCCAAAGATTTCTCCTTTTTCGATTGTTAATGAAACATCTTTTAATACt                                                         <  1:2396850/138‑1 (MQ=255)
                                                                                 cATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACt                                                         <  1:2622912/140‑1 (MQ=255)
                                                                                 cATCAAACAAAATCGTCACGCTGTCGGCGAAAATGATTTGCTTGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGAGTTCTGCTTTTGCAATTGTTAATGAATCATCTTTTAATACt                                                         >  2:1942879/1‑140 (MQ=255)
                                                                                           aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                                               >  1:1009925/1‑140 (MQ=255)
                                                                                           aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                                               >  1:2917916/1‑140 (MQ=255)
                                                                                           aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                                               >  2:2558716/1‑140 (MQ=255)
                                                                                           aaTCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGt                                               >  2:1209043/1‑140 (MQ=255)
                                                                                                tCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATc                                          >  1:475861/1‑140 (MQ=255)
                                                                                                tCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATc                                          >  1:940947/1‑140 (MQ=255)
                                                                                                     cTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGtttt                                     >  2:265603/1‑140 (MQ=255)
                                                                                                         cGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGAc                                 <  1:731278/140‑1 (MQ=255)
                                                                                                         cGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGAc                                 <  1:400956/140‑1 (MQ=255)
                                                                                                          ggCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACa                                >  1:552300/1‑140 (MQ=255)
                                                                                                           gCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACGTGATTTCCGTCAATCGTTTTTGACAg                               <  2:468535/140‑1 (MQ=255)
                                                                                                                                 aGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCCGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTc         >  1:2818121/1‑140 (MQ=255)
                                                                                                                                 aGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTACGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTc         >  2:1683405/1‑140 (MQ=255)
                                                                                                                                   cGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTa       <  1:1780022/140‑1 (MQ=255)
                                                                                                                                     aaGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACg     <  2:2618560/140‑1 (MQ=255)
                                                                                                                                     aaGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACg     <  1:1249742/140‑1 (MQ=255)
                                                                                                                                      aGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGTTTTCTCCTTTTTCAATTTTTAATGAAAAATCTTTTAATAGTTGATTTCCGTCAATCGTTTTTGACAGCTTCCGTATTTCAATAATTTTCTACgg    >  1:2651394/1‑139 (MQ=255)
                                                                                                                                      aGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGc    >  1:2212969/1‑140 (MQ=255)
                                                                                                                                      aGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGc    >  2:2351265/1‑140 (MQ=255)
                                                                                                                                       gCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGcc   >  2:979843/1‑140 (MQ=255)
                                                                                                                                       gCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGcc   >  2:1275634/1‑140 (MQ=255)
                                                                                                                                       gCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGcc   <  1:1212052/140‑1 (MQ=255)
                                                                                                                                        cATTGTCTTTTTGCCTGACCCATTGTGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTTTTAATGAAACATCTTTTAATACTTGATTTCGGTCAATCGTTTTTGACATCTGCCGTAGTTCAATCATTTTCTACGCCt  <  2:1986139/140‑1 (MQ=255)
                                                                                                                                        cATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTACTGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGCCt  <  2:374427/140‑1 (MQ=255)
                                                                                                                                        cATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGCCt  <  1:657830/140‑1 (MQ=255)
                                                                                                                                        cATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGCCt  <  2:2387605/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TATACTTGTCGTAAAAAGGATTTTGAACAGGCATGTAGATGATATTTTGTTTGACCTTCGGATGCTTTTTGATTTCTACGCCATCAAACAAAATCGTCCCGCTGTCGGCGAAAATGATTTGCTGGATTAAGCGAAGCATTGTCGTTTTGCCTGACCCATTGCGGCCGAGCAGCCCGAAGATTTCTCCTTTTTCAATTGTTAATGAAACATCTTTTAATACTTGATTTCCGTCAATCGTTTTTGACAGCTGCCGTAGTTCAATCATTTTCTACGCCT  >  NZ_CP034943/2920967‑2921242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: