Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,940,109 C→T 7.7% T88T (ACG→ACA EO946_RS15145 ← DNA starvation/stationary phase protection protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,940,1090CT 7.7% 572.2 / 26.7 182T88T (ACG→ACAEO946_RS15145DNA starvation/stationary phase protection protein
Reads supporting (aligned to +/- strand):  ref base C (96/71);  new base T (7/7);  total (104/78)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

TTCAATATTTTGATGGATCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAG  >  NZ_CP034943/2939971‑2940240
                                                                                                                                          |                                                                                                                                   
ttCAATATTTTGATGGATCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCg                                                                                                                                    >  2:1045014/1‑140 (MQ=255)
  cAATATTTTGATGGATCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGtt                                                                                                                                  >  1:2354944/1‑140 (MQ=255)
  cAATATTTTGATGGATCGCAAGAAGCATGTCCCCAGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCgat                                                                                                                                  >  1:1623653/1‑138 (MQ=255)
  cAATATTTTGATGGATCGAAATAATAATTTCCAATGTTTTTTCTTAACATAATTCATCAGATAAATCAAAGCAGTTTTTCATCTCTTAAGCAATGATTGTGAAATCATCATATACGCTTTGAAACATTTGTTCATACGtt                                                                                                                                  >  2:2460393/1‑140 (MQ=255)
                 tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                   >  1:2208434/1‑140 (MQ=255)
                 tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                   >  1:960316/1‑140 (MQ=255)
                 tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                   >  1:563738/1‑140 (MQ=255)
                           cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                         >  2:646479/1‑140 (MQ=255)
                           cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                         >  2:390037/1‑140 (MQ=255)
                           cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                         >  2:2782654/1‑140 (MQ=255)
                           cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                         >  2:2895192/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCGATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTGGTTGAGCCGTTTCATGTCATGCCGCTTCTTTTACAGAAGc                                                                                                     >  1:471959/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  1:2155347/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  1:2115214/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  2:2698915/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  2:277827/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  1:448249/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  2:718884/1‑140 (MQ=255)
                               tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATATCCTGCCGCTTCTTTTACAGAAGc                                                                                                     >  2:313957/1‑140 (MQ=255)
                                 cccTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCg                                                                                                   >  2:192497/1‑140 (MQ=255)
                                   cTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGtt                                                                                                 >  1:86671/1‑140 (MQ=255)
                                    tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                >  1:80232/1‑140 (MQ=255)
                                    tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                >  1:811928/1‑140 (MQ=255)
                                    tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                >  1:151179/1‑140 (MQ=255)
                                        gtatCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAg                                                                                            >  1:3036674/4‑140 (MQ=255)
                                         tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                           <  1:2193600/140‑1 (MQ=255)
                                         tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                           <  1:794107/140‑1 (MQ=255)
                                         tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                           >  2:2116074/1‑140 (MQ=255)
                                              tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                      >  1:977974/1‑140 (MQ=255)
                                              tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                      >  1:3111920/1‑140 (MQ=255)
                                                           caGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCa                                                                         <  1:288495/140‑1 (MQ=255)
                                                              cTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTACAGGGATTCCTTCATTGTCGCAATc                                                                      >  2:1762769/1‑140 (MQ=255)
                                                              cTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCAGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGCTTCATTCATTGTCGAAATc                                                                      >  1:227551/1‑140 (MQ=255)
                                                               tAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCg                                                                     <  2:1276795/140‑1 (MQ=255)
                                                                aaaTCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCgg                                                                    >  2:1309336/1‑140 (MQ=255)
                                                                      aTGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTCTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTcc                                                              >  2:2197539/1‑140 (MQ=255)
                                                                       tGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCg                                                             >  2:2048415/1‑140 (MQ=255)
                                                                       tGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTACg                                                             >  2:262665/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGTACCATTTGTTCAGCCGTTTCATTTCCTGCCGGTTGTTTTACAGAAGCCGTTTCCAGGGATTCCGTCATTGTCGCAATCGGTTTTCCGt                                                            >  1:1395216/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                            >  1:126052/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                            >  1:1241783/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                            >  2:597708/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                            >  2:1638347/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                            >  2:1063195/1‑140 (MQ=255)
                                                                        gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTACGt                                                            >  1:2074361/1‑140 (MQ=255)
                                                                            tttttCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAg                                                        <  2:563738/140‑1 (MQ=255)
                                                                                     tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                               <  2:2273856/140‑1 (MQ=255)
                                                                                     tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                               <  1:1076189/140‑1 (MQ=255)
                                                                                      ctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCTGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAAGCGGTTTTGCGTCCTGCGCCATAAGa                                              >  2:3088783/1‑140 (MQ=255)
                                                                                      ctTCCGCAATGATTGTGAAATCATCATATACGCGTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                              >  2:278494/1‑140 (MQ=255)
                                                                                        tCCGCAATGATTGTGAACTCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  1:1686098/140‑1 (MQ=255)
                                                                                        tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  2:965662/140‑1 (MQ=255)
                                                                                        tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  1:1638347/140‑1 (MQ=255)
                                                                                        tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  1:1953688/140‑1 (MQ=255)
                                                                                        tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  1:2858901/140‑1 (MQ=255)
                                                                                        tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTGGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGGCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                            <  2:1137867/140‑1 (MQ=255)
                                                                                          cGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                          <  1:2048980/140‑1 (MQ=255)
                                                                                                 aTTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCACTCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTaa                                   >  1:2384935/1‑140 (MQ=255)
                                                                                                     tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                               <  1:1035567/140‑1 (MQ=255)
                                                                                                      gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                              <  1:697966/140‑3 (MQ=255)
                                                                                                      gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                              <  1:646479/140‑3 (MQ=255)
                                                                                                      gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                              <  1:390036/140‑3 (MQ=255)
                                                                                                          tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                          <  1:1707922/140‑1 (MQ=255)
                                                                                                          tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                          <  2:2354944/140‑1 (MQ=255)
                                                                                                             tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                <  2:131319/99‑1 (MQ=255)
                                                                                                                     gCTTTGAACCCTCTGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:1855357/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  2:1372509/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:721224/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:1806693/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:597708/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:465987/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:2160585/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:277827/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:2845932/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:347625/140‑1 (MQ=255)
                                                                                                                     gCTTTGAACCATTTGGTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCCGTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg               <  1:309452/140‑1 (MQ=255)
                                                                                                                      cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAATATAAGTTGCCGt              >  2:890530/1‑140 (MQ=255)
                                                                                                                      cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt              >  2:2526051/1‑140 (MQ=255)
                                                                                                                      cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt              >  2:1841677/1‑140 (MQ=255)
                                                                                                                      cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt              >  2:850442/1‑140 (MQ=255)
                                                                                                                      cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGGCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt              >  2:525624/1‑140 (MQ=255)
                                                                                                                       tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCGAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtg             >  1:1856369/1‑139 (MQ=255)
                                                                                                                       tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt             <  1:1050752/140‑1 (MQ=255)
                                                                                                                       tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt             <  1:1045014/140‑1 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCCTTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt            >  2:434214/1‑140 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAATCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt            >  1:3026546/1‑140 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAATCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt            >  2:1736502/1‑140 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt            >  1:1758404/1‑140 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt            <  1:1156317/140‑1 (MQ=255)
                                                                                                                        ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACCTTCTGATAAGTCGTCAATATAAGTTGCCGttt            >  2:1804778/1‑140 (MQ=255)
                                                                                                                          gAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCg          <  1:1418662/140‑1 (MQ=255)
                                                                                                                          gAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTGTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCg          <  1:2460393/140‑1 (MQ=255)
                                                                                                                           aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt         >  2:2995379/1‑140 (MQ=255)
                                                                                                                           aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt         >  2:2224886/1‑140 (MQ=255)
                                                                                                                                tttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGACGTTTCGTTATAc    >  2:2669286/1‑140 (MQ=255)
                                                                                                                                 ttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTACGTTATACa   >  2:1407485/1‑140 (MQ=255)
                                                                                                                                  tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg  <  2:1443298/140‑1 (MQ=255)
                                                                                                                                  tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg  <  2:1573701/140‑1 (MQ=255)
                                                                                                                                  tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg  <  2:3111920/140‑1 (MQ=255)
                                                                                                                                  tGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg  <  1:262665/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                   
TTCAATATTTTGATGGATCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAG  >  NZ_CP034943/2939971‑2940240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: