Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,940,121 T→A 7.6% A84A (GCA→GCT EO946_RS15145 ← DNA starvation/stationary phase protection protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,940,1210TA 7.6% 612.7 / 30.2 183A84A (GCA→GCTEO946_RS15145DNA starvation/stationary phase protection protein
Reads supporting (aligned to +/- strand):  ref base T (98/71);  new base A (7/7);  total (105/78)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 183 total aligned reads displayed.

TCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTC  >  NZ_CP034943/2939988‑2940255
                                                                                                                                     |                                                                                                                                      
tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                                  >  1:563035/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                        >  2:646479/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                        >  2:390037/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                        >  2:2895192/1‑140 (MQ=255)
            tGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGaa                                                                                                                      >  2:1095865/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGACATCATCATATACGCTTTGAACCATTTGTTCAGCCGGTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  1:1727847/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  2:718884/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  1:965662/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  1:448249/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  1:2292865/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  2:2698915/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  2:277827/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATATCCTGCCGCTTCTTTTACAGAAGc                                                                                                                    >  2:313957/1‑140 (MQ=255)
                cccTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCg                                                                                                                  >  2:2458932/1‑140 (MQ=255)
                  cTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGtt                                                                                                                >  1:86671/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                               >  1:80232/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                               >  1:811928/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                               >  1:151179/1‑140 (MQ=255)
                    gttgttTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTc                                                                                                              >  1:3053273/1‑140 (MQ=255)
                       gtatCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAg                                                                                                           >  1:3036674/4‑140 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                          <  1:794107/140‑1 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                          >  2:1035567/1‑140 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                          <  1:2193600/140‑1 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTAAAGAATCCGTTTCCAgg                                                                                                          >  1:2111053/1‑140 (MQ=255)
                          tCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGa                                                                                                        <  1:2427713/140‑1 (MQ=255)
                          tCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGGTTCATTTCCTGCCGCTTCTGTTACAGAAGCCGTTTCCAGGGa                                                                                                        >  1:1765038/1‑140 (MQ=255)
                             tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                     >  1:1822215/1‑140 (MQ=255)
                                          caGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCa                                                                                        <  1:288495/140‑1 (MQ=255)
                                              tAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCg                                                                                    <  2:2115214/140‑1 (MQ=255)
                                               aaaTCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCgg                                                                                   >  2:1309336/1‑140 (MQ=255)
                                                     aTGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTCTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTcc                                                                             >  2:2197539/1‑140 (MQ=255)
                                                      tGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTACg                                                                            >  2:262665/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGTACCATTTGTTCAGCCGTTTCATTTCCTGCCGGTTGTTTTACAGAAGCCGTTTCCAGGGATTCCGTCATTGTCGCAATCGGTTTTCCGt                                                                           >  1:1395216/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  2:1638347/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  2:128446/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  1:2273856/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  1:126052/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  1:1241783/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                           >  2:597708/1‑140 (MQ=255)
                                                           tttttCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAg                                                                       <  2:563738/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                              <  2:2273856/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                              <  1:1076189/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                              <  1:887109/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                              <  2:126052/140‑1 (MQ=255)
                                                                     ctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCTGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAAGCGGTTTTGCGTCCTGCGCCATAAGa                                                             >  2:3088783/1‑140 (MQ=255)
                                                                     ctTCCGCAATGATTGTGAAATCATCATATACGCGTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                                             >  2:278494/1‑140 (MQ=255)
                                                                       tCCGCAATGATTGTGAACTCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                           <  1:1686098/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                           <  2:965662/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                           <  1:1953688/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                           <  1:1702048/140‑1 (MQ=255)
                                                                         cGCAATGATTGTGAAATCATCATATACGCTCTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                                         <  1:2197539/140‑1 (MQ=255)
                                                                                    tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                                              <  1:1035567/140‑1 (MQ=255)
                                                                                    tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                                              <  1:2116074/140‑1 (MQ=255)
                                                                                     gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                             <  1:390036/140‑3 (MQ=255)
                                                                                     gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                             <  1:2782654/140‑3 (MQ=255)
                                                                                         tgatcatATACGCTTTGAACCATTTGTTCAGACTTTTCATTTCCTGCCGCTTCTTTTACAGAAGCAGTTTCCAGGGATTCCTTCATTGTCGCAATCGTTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAat                                         <  2:1623653/138‑1 (MQ=255)
                                                                                         tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                                         <  2:2354944/140‑1 (MQ=255)
                                                                                            tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                               >  1:3107339/1‑99 (MQ=255)
                                                                                            tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                               <  2:131319/99‑1 (MQ=255)
                                                                                              atatACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAg                                    <  1:1965955/140‑1 (MQ=255)
                                                                                                 taCGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTg                                 >  2:1961334/1‑140 (MQ=255)
                                                                                                 taCGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTg                                 >  2:178057/1‑140 (MQ=255)
                                                                                                   cGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGcc                               >  1:2053853/1‑140 (MQ=255)
                                                                                                    gCTTTGAACCCTTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGGTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:687700/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:1627052/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:2160585/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:718884/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:2698915/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:465987/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:721224/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:597708/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  1:347625/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                              <  2:1372509/140‑1 (MQ=255)
                                                                                                    ccgttGCACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGACAAAAGACGTTCTGCTAAGTCTTCAATATAAGTTGCCg                              <  1:2845967/137‑1 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAATATAAGTTGCCGt                             >  2:890530/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                             >  2:850442/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                             >  2:2526051/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGGCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                             >  2:525624/1‑140 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCGAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtg                            >  1:1856369/1‑139 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                            <  1:1045014/140‑1 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                            <  1:1050752/140‑1 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                            >  1:630065/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCCTTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                           >  2:434214/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAATCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                           >  1:3026546/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAATCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                           >  2:1736502/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                           <  1:1156317/140‑1 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                           <  1:471848/140‑1 (MQ=255)
                                                                                                         gAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCg                         <  1:1418662/140‑1 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                        >  2:2224886/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                        >  2:2995379/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                        >  2:2779654/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                        >  2:1539142/1‑140 (MQ=255)
                                                                                                               tttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTGAATATAAGTTGCCGTTTCGTtataa                   >  2:191670/1‑139 (MQ=255)
                                                                                                               tttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGACGTTTCGTTATAc                   >  2:2669286/1‑140 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                 <  2:3111920/140‑1 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                 <  2:1822215/140‑1 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                 <  2:1414085/140‑1 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                 <  1:262665/140‑1 (MQ=255)
                                                                                                                            ttCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACt      >  2:1972968/1‑140 (MQ=255)
                                                                                                                                tttCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCGTCATTGTCGCAATCGGTTTTCCGTCCCCCGCCAAAAGACGTTCTGCTAAGTCGTCAAAATAAGTTGCCGGTTCGTTATACAGCTCCGCAAAAATTTc  >  1:2514684/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                      
TCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTC  >  NZ_CP034943/2939988‑2940255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: