Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 452,125 T→A 100% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA100.0% 422.3 / ‑6.6 128K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (66/61);  total (66/62)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 130 total aligned reads displayed.

TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261
                                                                                                                                         |                                                                                                                                        
tttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGtatt                                                                                                                                        <  2:576354/140‑1 (MQ=255)
tttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAACAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGGATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGtatt                                                                                                                                        <  2:1888823/140‑1 (MQ=255)
      ccTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATa                                                                                                                                  <  1:1516397/140‑1 (MQ=255)
        tagTTTTTGATTATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAACTTTGGATGCTTTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  1:3090720/138‑1 (MQ=255)
              ttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGc                                                                                                                          <  2:2159326/140‑1 (MQ=255)
              ttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGc                                                                                                                          <  2:2640357/140‑1 (MQ=255)
              ttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGc                                                                                                                          <  2:60454/140‑1 (MQ=255)
                tatgatgGAAAGCGTTTTTAAGAATAGTGGCCTGTAAAAATAAACCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCaa                                                                                                                        <  2:867243/139‑1 (MQ=255)
                  tgattGAAAGCGTTTTGACGAAGAGTGGCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCACATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACa                                                                                                                      <  1:2283691/140‑1 (MQ=255)
                          aGCTTTTTTAAGAAGAGTCCCATGTCAAAATAAACCGACACGGGATGGGAAAAGCCATGAAGAGCATGAAATTGATACATCTCCAAATTGGGATGCTGTTGTTCCCTCCGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:2546309/140‑1 (MQ=255)
                          aGCTTTTTGAAGAAGAGTGGCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:2295775/140‑1 (MQ=255)
                          aGCTTTTTGAAGAAGAGTGGCCTGAAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  1:767626/140‑1 (MQ=255)
                          aGCGTTTTTACGAAGATTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:2388011/140‑1 (MQ=255)
                          aGCGTTTTGAAGAAGAGTGGCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGCAGCGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:163002/140‑1 (MQ=255)
                          aGCGTTTTGAAGAAGAGTGGCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:159738/140‑1 (MQ=255)
                           gctttttCAAGAAGAGGGCCCTCAAAAAATAACCCGACTCGGTATTGGAAAAGCCATGAAGACCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacgac                                                                                                             <  1:2574744/137‑1 (MQ=255)
                              ttttGAAGAAGTGTGGCATGTCAAAATAAACCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAAAATCAAACGACGGt                                                                                                          <  2:2376926/140‑1 (MQ=255)
                               ttttAAGACGTGGGGCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGtt                                                                                                         <  1:1885049/140‑1 (MQ=255)
                               tttGAAGAAGAGTGGCCAGAAAAAATAAACCGACTCGGGTTTGGCAAAGCCATGAAGAGCATAAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGtt                                                                                                         <  1:1648947/140‑1 (MQ=255)
                                ttGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATGTTGATAAGAACCGCAACATCAAACGACGGTTc                                                                                                        >  1:789767/1‑140 (MQ=255)
                                     gaagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATg                                                                                                   <  2:667712/140‑1 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                  >  1:2824284/1‑140 (MQ=255)
                                        gagTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAg                                                                                                >  2:642174/1‑140 (MQ=255)
                                             gccttaaaaaaTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:1495580/133‑1 (MQ=255)
                                             gCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:857135/140‑1 (MQ=255)
                                             gCCTTTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:1203062/140‑1 (MQ=255)
                                             gCCTGTCAAAATACCCCGACACGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAAACCCCACATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:1394337/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:1465171/140‑1 (MQ=255)
                                             gCCTGTAAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:2011973/140‑1 (MQ=255)
                                             gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:941395/140‑1 (MQ=255)
                                             gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:841876/140‑1 (MQ=255)
                                             gCCTGACAAAATAACCCGACTCGGGATTGGCAACGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAAAATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:943778/140‑1 (MQ=255)
                                             gCCTCTAAAAATAACCCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:88913/140‑1 (MQ=255)
                                                tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                        >  1:1133760/1‑140 (MQ=255)
                                                   cAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAAt                                                                                     >  2:2659039/1‑140 (MQ=255)
                                                                     gATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                   <  1:1954857/140‑1 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                         <  1:1721746/140‑1 (MQ=255)
                                                                               gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATAGGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                         >  1:1031159/1‑140 (MQ=255)
                                                                                  aTGAAGACCACCACAGTGAGAAATCTCCACAGTGCGATGCTGTGGGTCCCTCATTATATTGAGACCAACCGCACCATCAAACGACGGTTCATATGCAGGGAAAACCAATAGGACCGGGCAGACTGGCACGTGATGAagag                                                      <  2:1241937/140‑1 (MQ=255)
                                                                                   tGACGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAgaga                                                     <  1:1331972/140‑1 (MQ=255)
                                                                                   tGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAgaga                                                     <  1:2588035/140‑1 (MQ=255)
                                                                                    gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGGGATGAAGAGaa                                                    >  2:1820849/1‑140 (MQ=255)
                                                                                    gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGGATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                    >  2:164596/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAATAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGGGATGACGAGAATCGCGt                                              >  2:729776/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGAGCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2951905/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGACTCGCGt                                              >  2:952627/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  2:808317/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  2:865935/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  2:2545685/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:810301/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  2:709204/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:1096216/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2531351/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2888037/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2216619/1‑140 (MQ=255)
                                                                                          cATCAAATTGATAAATCTCCAAAGTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAATAGAATCTCGt                                              >  1:2318022/1‑140 (MQ=255)
                                                                                           aTCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTa                                             >  1:302067/1‑140 (MQ=255)
                                                                                           aTCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTa                                             >  1:2960390/1‑140 (MQ=255)
                                                                                           aTCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTa                                             >  1:2960446/1‑140 (MQ=255)
                                                                                           aTCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTCGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTa                                             >  1:1856272/1‑140 (MQ=255)
                                                                                                           tcCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGtaata                             <  2:1031159/140‑1 (MQ=255)
                                                                                                                tttGGGATGCTGTTGTTCCCTCAGTATTTTTATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaacaaa                        <  1:2031788/139‑1 (MQ=255)
                                                                                                                aTTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaacaaa                        <  1:236297/140‑1 (MQ=255)
                                                                                                                   gggATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAg                     <  1:837249/140‑1 (MQ=255)
                                                                                                                        gCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCg                <  1:1373986/140‑1 (MQ=255)
                                                                                                                          tgttttTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:1961349/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:1441896/140‑1 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  2:1008456/1‑140 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  2:1278401/1‑140 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  2:14951/1‑140 (MQ=255)
                                                                                                                          tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              >  2:1039233/1‑140 (MQ=255)
                                                                                                                          tggtgttCCCTCAGTATTTTGATAAGAACCGCAAGCTCACACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACAGTAATAACAAAccgctccgct              <  1:295317/137‑1 (MQ=255)
                                                                                                                           ggtgttCCCTCAGTATTTTGATAAGAACCGCACCACCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTc             <  2:1919631/138‑1 (MQ=255)
                                                                                                                             tgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  1:2628729/140‑1 (MQ=255)
                                                                                                                              gttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAcc          >  1:1849621/1‑140 (MQ=255)
                                                                                                                               ttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCt         <  1:2986635/140‑1 (MQ=255)
                                                                                                                                 cccTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa       >  1:2795946/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTACTAACAAACAGCTCCGCTCACCTCAGGc    >  1:2985860/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAgga    >  2:560589/1‑139 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1727706/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1568061/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1540202/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:838758/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1279145/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:1142435/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:532686/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:2129811/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:2129869/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:2492278/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:1070452/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:339034/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:706209/1‑140 (MQ=255)
                                                                                                                                    tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAACGGAAAAACAATAGGACCTGGCAGACTGGCACGTGATGTAGAGAATCGCGTATCCGTGAACCGTAATAACAAACAGCTCAGCTCACCTCAgtc    >  2:143769/1‑138 (MQ=255)
                                                                                                                                     cAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa   <  1:2100564/140‑1 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  2:2782550/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  1:2365037/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  2:2192755/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  2:1938119/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  2:1384794/1‑140 (MQ=255)
                                                                                                                                      aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  1:835009/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: