Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,940,124 C→A 7.5% A83A (GCG→GCT EO946_RS15145 ← DNA starvation/stationary phase protection protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,940,1240CA 7.5% 558.4 / 25.7 187A83A (GCG→GCTEO946_RS15145DNA starvation/stationary phase protection protein
Reads supporting (aligned to +/- strand):  ref base C (102/71);  new base A (7/7);  total (109/78)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 187 total aligned reads displayed.

TCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAAG  >  NZ_CP034943/2939988‑2940261
                                                                                                                                        |                                                                                                                                         
tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                                        >  1:2208434/1‑140 (MQ=255)
tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                                        >  1:563035/1‑140 (MQ=255)
tCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGct                                                                                                                                        >  1:960316/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                              >  2:646479/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                              >  2:390037/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                              >  2:2562355/1‑140 (MQ=255)
          cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                              >  2:2895192/1‑140 (MQ=255)
            tGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGaa                                                                                                                            >  2:1095865/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGTAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  2:1312765/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  2:718884/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  1:2155347/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  1:2115214/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  1:965662/1‑140 (MQ=255)
              tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATATCCTGCCGCTTCTTTTACAGAAGc                                                                                                                          >  2:313957/1‑140 (MQ=255)
                cccTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCg                                                                                                                        >  2:2458932/1‑140 (MQ=255)
                cccTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCg                                                                                                                        >  2:192497/1‑140 (MQ=255)
                  cTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGtt                                                                                                                      >  1:86671/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                     >  1:151179/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                     >  2:1357142/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                     >  1:811928/1‑140 (MQ=255)
                   tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                     >  1:80232/1‑140 (MQ=255)
                    gttgttTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTc                                                                                                                    >  1:3053273/1‑140 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                                >  2:1035567/1‑140 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                                <  1:794107/140‑1 (MQ=255)
                        tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                                >  2:2116074/1‑140 (MQ=255)
                             tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                           >  1:1414085/1‑140 (MQ=255)
                             tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                           <  2:3036674/140‑1 (MQ=255)
                                          caGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCa                                                                                              <  1:288495/140‑1 (MQ=255)
                                             cTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTACAGGGATTCCTTCATTGTCGCAATc                                                                                           >  2:1762769/1‑140 (MQ=255)
                                             cTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCAGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGCTTCATTCATTGTCGAAATc                                                                                           >  1:227551/1‑140 (MQ=255)
                                                      tGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCg                                                                                  >  2:2048415/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                 >  2:128446/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                 >  2:163947/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                 >  1:1241783/1‑140 (MQ=255)
                                                       gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTACGt                                                                                 >  1:2074361/1‑140 (MQ=255)
                                                           tttttCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAg                                                                             <  2:2208434/140‑1 (MQ=255)
                                                           tttttCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAg                                                                             <  2:563738/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATCCGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                    <  1:2177460/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                    <  2:126052/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                    <  1:1076189/140‑1 (MQ=255)
                                                                    tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                    <  1:45404/140‑1 (MQ=255)
                                                                     ctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                                                   >  1:3029536/1‑140 (MQ=255)
                                                                     ctTCCGCAATGATTGTGAAATCATCATATACGCGTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                                                   >  2:278494/1‑140 (MQ=255)
                                                                       tCCGCAATGATTGTGAACTCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                 <  1:1686098/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                 <  2:569501/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                 <  2:2695685/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                 <  2:965662/140‑1 (MQ=255)
                                                                       tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                 <  1:1953688/140‑1 (MQ=255)
                                                                         cGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                                               <  1:2048980/140‑1 (MQ=255)
                                                                         cGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                                               <  1:388652/140‑1 (MQ=255)
                                                                         cGCAATGATTGTGAAATCATCATATACGCTCTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                                               <  1:2197539/140‑1 (MQ=255)
                                                                                aTTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCACTCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTaa                                                        >  1:2384935/1‑140 (MQ=255)
                                                                                aTTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTaa                                                        >  2:2251114/1‑140 (MQ=255)
                                                                                    tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                                                    <  1:1035567/140‑1 (MQ=255)
                                                                                     gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                                   <  1:390037/140‑3 (MQ=255)
                                                                                     gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                                   <  1:697966/140‑3 (MQ=255)
                                                                                     gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                                   <  1:2782654/140‑3 (MQ=255)
                                                                                     caaaTCATCCTATACGCTTTCAACCCCTGGGTCAGCCGTTTCATTTCCTGCCGCTTCTTTTTCAGAAGCCGTTTCCAGGGATTCCTTCAATGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACCTTCTGCTAAgtcgt                                                   <  2:2296959/139‑1 (MQ=255)
                                                                                         tgatcatATACGCTTTGAACCATTTGTTCAGACTTTTCATTTCCTGCCGCTTCTTTTACAGAAGCAGTTTCCAGGGATTCCTTCATTGTCGCAATCGTTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAat                                               <  2:1623653/138‑1 (MQ=255)
                                                                                         tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                                               <  1:1707922/140‑1 (MQ=255)
                                                                                            tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                     <  2:3107339/99‑1 (MQ=255)
                                                                                            tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                     >  1:131319/1‑99 (MQ=255)
                                                                                            tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                     >  1:3107339/1‑99 (MQ=255)
                                                                                                 taCGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTg                                       >  2:1961334/1‑140 (MQ=255)
                                                                                                    gCTTTGAACCCTTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGGTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:687700/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCCTCTGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:1855357/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:1627052/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:347625/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:1806693/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:721224/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:597708/140‑1 (MQ=255)
                                                                                                    gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                    <  1:277827/140‑1 (MQ=255)
                                                                                                    ccgttGCACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGACAAAAGACGTTCTGCTAAGTCTTCAATATAAGTTGCCg                                    <  1:2845967/137‑1 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAATATAAGTTGCCGt                                   >  2:890530/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                   >  2:2654423/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                   >  2:850442/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                   >  2:1841677/1‑140 (MQ=255)
                                                                                                     cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGGCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                   >  2:525624/1‑140 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCGAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtg                                  >  1:1856369/1‑139 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                  <  1:1045014/140‑1 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                  <  1:1050752/140‑1 (MQ=255)
                                                                                                      tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                  >  1:630065/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCCTTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                 >  2:434214/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                 <  1:1156317/140‑1 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                 >  1:1758404/1‑140 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                 <  1:471848/140‑1 (MQ=255)
                                                                                                       ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACCTTCTGATAAGTCGTCAATATAAGTTGCCGttt                                 >  2:1804778/1‑140 (MQ=255)
                                                                                                         gAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCg                               <  1:1418662/140‑1 (MQ=255)
                                                                                                         gAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTGTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCg                               <  1:2460393/140‑1 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                              >  2:2995372/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                              >  2:1539142/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                              >  2:2779654/1‑140 (MQ=255)
                                                                                                          aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                              >  2:275403/1‑140 (MQ=255)
                                                                                                                ttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTACGTTATACa                        >  2:1407485/1‑140 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                       <  2:1414085/140‑1 (MQ=255)
                                                                                                                 tGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                       <  1:262665/140‑1 (MQ=255)
                                                                                                                            ttCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACt            >  2:2520302/1‑140 (MQ=255)
                                                                                                                                tttCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCGTCATTGTCGCAATCGGTTTTCCGTCCCCCGCCAAAAGACGTTCTGCTAAGTCGTCAAAATAAGTTGCCGGTTCGTTATACAGCTCCGCAAAAATTTc        >  1:2514684/1‑140 (MQ=255)
                                                                                                                                      gCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAag  >  2:822019/1‑140 (MQ=255)
                                                                                                                                      gCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAag  >  1:2973725/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TCGCAAGAAGCATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAAG  >  NZ_CP034943/2939988‑2940261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: