Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,940,133 T→G 7.5% V80V (GTA→GTC EO946_RS15145 ← DNA starvation/stationary phase protection protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,940,1330TG 7.5% 575.3 / 23.1 186V80V (GTA→GTCEO946_RS15145DNA starvation/stationary phase protection protein
Reads supporting (aligned to +/- strand):  ref base T (101/71);  new base G (7/7);  total (108/78)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 188 total aligned reads displayed.

CATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAAGAGTG  >  NZ_CP034943/2939998‑2940265
                                                                                                                                       |                                                                                                                                    
cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                                  >  2:646479/1‑140 (MQ=255)
cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                                  >  2:390037/1‑140 (MQ=255)
cATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCGATGACTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAg                                                                                                                                  >  2:2562355/1‑140 (MQ=255)
  tGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGaa                                                                                                                                >  2:2858901/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGTAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  2:1312765/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  1:965662/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  1:448249/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  2:718884/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  1:2292865/1‑140 (MQ=255)
    tCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATATCCTGCCGCTTCTTTTACAGAAGc                                                                                                                              >  2:313957/1‑140 (MQ=255)
      cccTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCg                                                                                                                            >  2:192497/1‑140 (MQ=255)
        cTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGtt                                                                                                                          >  1:86671/1‑140 (MQ=255)
         tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                         >  1:811928/1‑140 (MQ=255)
         tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                         >  1:151179/1‑140 (MQ=255)
         tgttgtTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGttt                                                                                                                         >  2:1357142/1‑140 (MQ=255)
          gttgttTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTc                                                                                                                        >  1:3053273/1‑140 (MQ=255)
              tttCGTCACCGACTTCATCATCTAAATCCATGCCGATTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                                    <  1:793481/140‑1 (MQ=255)
              tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAgg                                                                                                                    <  1:2193600/140‑1 (MQ=255)
              tttCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTAAAGAATCCGTTTCCAgg                                                                                                                    >  1:2111053/1‑140 (MQ=255)
                tCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGa                                                                                                                  <  1:2427713/140‑1 (MQ=255)
                   tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                               >  1:1414085/1‑140 (MQ=255)
                   tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                               >  1:977974/1‑140 (MQ=255)
                   tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                               >  1:1443298/1‑140 (MQ=255)
                   tCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTc                                                                                                               <  2:3036674/140‑1 (MQ=255)
                                caGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCa                                                                                                  <  1:288495/140‑1 (MQ=255)
                                    tAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCg                                                                                              <  2:1276795/140‑1 (MQ=255)
                                    tAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCg                                                                                              <  2:2115214/140‑1 (MQ=255)
                                     aaaTCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCgg                                                                                             >  2:1309336/1‑140 (MQ=255)
                                           aTGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTCTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTcc                                                                                       >  2:2197539/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGGCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTGCTTCATTGTCGCAATCGGTTTTCCGt                                                                                     >  1:79595/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                     >  2:1063195/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                     >  1:1241783/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                     >  1:2273856/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGt                                                                                     >  2:1638347/1‑140 (MQ=255)
                                             gCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTACGt                                                                                     >  1:2074361/1‑140 (MQ=255)
                                                 tttttCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAg                                                                                 <  2:563035/140‑1 (MQ=255)
                                                          tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                        <  1:1076189/140‑1 (MQ=255)
                                                          tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                        <  1:45404/140‑1 (MQ=255)
                                                          tctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAg                                                                        <  2:2273856/140‑1 (MQ=255)
                                                           ctTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                                                       >  1:3029536/1‑140 (MQ=255)
                                                           ctTCCGCAATGATTGTGAAATCATCATATACGCGTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGa                                                                       >  2:278494/1‑140 (MQ=255)
                                                             tCCGCAATGATTGTGAACTCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  1:1686098/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  1:2858901/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  2:2695685/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  2:569501/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  2:965662/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  1:1953688/140‑1 (MQ=255)
                                                             tCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTGGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGGCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACg                                                                     <  2:1137867/140‑1 (MQ=255)
                                                               cGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGtt                                                                   <  1:2048980/140‑1 (MQ=255)
                                                                          tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                                                        <  1:1035567/140‑1 (MQ=255)
                                                                          tgAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAgtcg                                                        <  1:2116074/140‑1 (MQ=255)
                                                                           gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                                       <  1:390036/140‑3 (MQ=255)
                                                                           gAAATCATCATATACGCTTTGAACCATTTGTTCAGCTGTTTCATTTCCAGCAGCTTCTTTGACAGAAGCCGTTTCTAAGGATTCCTTCATTGTTGCAATCGGTTTTCCGTTCAGCGCCAAAAGACGTTCTGCTAAgtcat                                                       <  1:2562355/140‑3 (MQ=255)
                                                                           caaaTCATCCTATACGCTTTCAACCCCTGGGTCAGCCGTTTCATTTCCTGCCGCTTCTTTTTCAGAAGCCGTTTCCAGGGATTCCTTCAATGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACCTTCTGCTAAgtcgt                                                       <  2:2296959/139‑1 (MQ=255)
                                                                               tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                                                   <  2:2354944/140‑1 (MQ=255)
                                                                               tcatcaTATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAat                                                   <  1:1707922/140‑1 (MQ=255)
                                                                                  tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                         <  2:3107339/99‑1 (MQ=255)
                                                                                  tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                         >  1:131319/1‑99 (MQ=255)
                                                                                  tcatATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGtttt                                                                                         >  1:3107339/1‑99 (MQ=255)
                                                                                       taCGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTg                                           >  2:178057/1‑140 (MQ=255)
                                                                                          gCTTTGAACCCTTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGGTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:687700/140‑1 (MQ=255)
                                                                                          gCTTTGAACCCTCTGTTCAGCCGTTTCATTTCCTGCCGCGTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:1855357/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:718884/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:1627052/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:1806693/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:2160585/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:277827/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:2845932/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:347625/140‑1 (MQ=255)
                                                                                          gCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:597708/140‑1 (MQ=255)
                                                                                           cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAATTCGTCAATATAAGTTGCCGt                                       >  2:890530/1‑140 (MQ=255)
                                                                                           cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                       >  2:2654423/1‑140 (MQ=255)
                                                                                           cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                       >  2:1841677/1‑140 (MQ=255)
                                                                                           cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                       >  2:850442/1‑140 (MQ=255)
                                                                                           cTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGGCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGt                                       >  2:525624/1‑140 (MQ=255)
                                                                                            tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                      <  1:1045014/140‑1 (MQ=255)
                                                                                            tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                      >  1:630065/1‑140 (MQ=255)
                                                                                            tttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGtt                                      <  1:1050752/140‑1 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCCTTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     >  2:434214/1‑140 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAATCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     >  2:1736502/1‑140 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     <  1:1156317/140‑1 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     >  1:1758404/1‑140 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     <  1:471848/140‑1 (MQ=255)
                                                                                             ttGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCAGTCCAGCGCCAAAAGACCTTCTGATAAGTCGTCAATATAAGTTGCCGttt                                     >  2:1804778/1‑140 (MQ=255)
                                                                                             gtGAACCCTTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATGGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGttt                                     <  1:1889785/139‑1 (MQ=255)
                                                                                                aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                                  >  2:2995372/1‑140 (MQ=255)
                                                                                                aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                                  >  2:2224886/1‑140 (MQ=255)
                                                                                                aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                                  >  2:2779654/1‑140 (MQ=255)
                                                                                                aaCCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGt                                  >  2:275403/1‑140 (MQ=255)
                                                                                                      ttGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTACGTTATACa                            >  2:1407485/1‑140 (MQ=255)
                                                                                                       tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                           <  2:1443298/140‑1 (MQ=255)
                                                                                                       tGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAg                           <  2:1573701/140‑1 (MQ=255)
                                                                                                                  ttCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACt                >  2:1972968/1‑140 (MQ=255)
                                                                                                                  ttCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACt                >  2:2520302/1‑140 (MQ=255)
                                                                                                                      tttCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTTTCGGAATCGGTTTTGCGTCCCGCGCCAAAAGACGGTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGGTATACAGCTCCTGAAACttttg            >  1:803869/1‑139 (MQ=255)
                                                                                                                            gCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAag      >  1:2973725/1‑140 (MQ=255)
                                                                                                                            gCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAag      >  1:2492944/1‑140 (MQ=255)
                                                                                                                            gCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAag      >  2:822019/1‑140 (MQ=255)
                                                                                                                               gCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAAGAGt   >  1:2638618/1‑140 (MQ=255)
                                                                                                                                     tttACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCg                                        <  1:2289078/97‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
CATGTCCCCTGTTGTTTCGTCACCGACTTCATCAGCTAAATCCATGCCGTTTTTCAGCTCTTCCGCAATGATTGTGAAATCATCATATACGCTTTGAACCATTTGTTCAGCCGTTTCATTTCCTGCCGCTTCTTTTACAGAAGCCGTTTCCAGGGATTCCTTCATTGTCGCAATCGGTTTTCCGTCCAGCGCCAAAAGACGTTCTGCTAAGTCGTCAATATAAGTTGCCGTTTCGTTATACAGCTCCTCAAACTTTTCATGAAGAGTG  >  NZ_CP034943/2939998‑2940265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: