| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_CP034943 | 2,980,160 | G→A | 29.2% | noncoding (641/2928 nt) | EO946_RS15430 ← | 23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 2,980,160 | 0 | G | A | 29.2% | ‑3.7 / 15.2 | 24 | noncoding (641/2928 nt) | EO946_RS15430 | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (3/14); new base A (2/5); total (5/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
CGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC > NZ_CP034943/2980021‑2980296 | cgtgCCTCCTTTAATTGTTAACTGAAATTCTCGCTGGACATGTTTTCATCACCTGGTTTCGGTTCTACTACCACTTACTCATTCGCCCTATTCATACTCGCTTTCGCTGCGGCTACGCATCTTCTGCTTAACCTTGCACg < 2:2692520/137‑1 (MQ=0) gatcTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCAtt < 2:2779475/137‑1 (MQ=1) cttgACATTGGTAGATCACCTGGTTTCGTGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCTCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTGGCCGTTTCATTCTACAAAAgg < 2:255945/137‑1 (MQ=1) tgcaCATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGAGCCCTATTCAGACTCGATTTCGCTGCGGCTCCGCATCTTCTGCTTAACATTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAgtc > 1:1570070/4‑138 (MQ=1) gagaTGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTATCCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAATAGGCAc > 1:2596497/4‑140 (MQ=0) ggctAGATCACCTGGTTTCGGGTCTACGACCACGTTCTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCAt < 2:299380/137‑1 (MQ=1) ccgggTTTCGGGTCTACGACCACGTACTCATGCGCCGTATTCATACTGGCTTTCGCTTCGGCTCCGGATGTTCTGCTTAACCTTTCTCAGGATCGTAACTCGGCTGTTCATTCTACAAAAGGCACGCCATCACCCGTTaa < 2:39896/137‑1 (MQ=1) ccgggTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACGCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTaa < 1:2470250/137‑1 (MQ=1) ccgggTGTCGGGTCTACGACCACGCACTCAGGCGCCCTCTTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTaa < 2:1291760/137‑1 (MQ=1) gtgtCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACATTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACggg < 1:1201037/137‑1 (MQ=1) gtatACGACCACGTACTAATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGac < 1:1425439/137‑1 (MQ=0) acttACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCTCATCTTCTGCTTAACCTTGCAGGGTATCGTAACTCGCCGGTTCATTATACAAAAGGCACTCCATCACCCGTTAACGGGCTCTGACTACTTGTAgg < 1:1648349/137‑1 (MQ=1) acccaggcgcCCTAGTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCGGGCACAGGATCGGACCCCGCCGGTTCATTCTACAAAAGGCCCGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGcaca < 2:1705658/134‑1 (MQ=1) tagtCAGACTCGCTGTCGCTGCGGCTCCGCATGTTCTGCGGAAGCTTGCACGGGCTCGTACCGCGGCGGTTCATTCTACAAAAGGCACGCCCTCACCCGTTAACGGGCTCTGACTACTTGTAGGCACAGGGTTTCAGGAt < 2:2076964/137‑1 (MQ=1) gcgttCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTc < 2:2950731/137‑1 (MQ=1) ctgtCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcc > 2:2969214/4‑140 (MQ=1) ctgtCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcc > 2:502016/4‑140 (MQ=1) ctctCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcc > 1:796463/4‑140 (MQ=1) gtctcTGCGGCTCCGCATCTTCTGCTTAACCTTGCACAGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCAGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTcccc < 2:1570156/136‑1 (MQ=0) gatattctGCTTAACCTTGCACGTGAACGTAACTCTCCGGTTCATTCTACAACAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCtttt < 1:3060013/136‑1 (MQ=1) gttatGCTTAACCTTGCACGGGATCGTAAGTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTATCGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATATCTTTCACTCCCCTTCCGGGGTGCTTTTCAc < 1:1762183/136‑1 (MQ=0) cccgtGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCCTTAACGGGCTCTGACTACGTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa < 2:2368195/136‑1 (MQ=0) accgttcACGCGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCTTTAACGGGCTCTGACTACTTGTCGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa < 2:1644612/134‑1 (MQ=1) ccgtGCACGGGATCGTAACTCGCGGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCCCACCGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc < 1:2095623/137‑1 (MQ=0) ccgtGCACGGGATCGTAACTCGCCGGGTCATTCTACACAAGGCCCGCCCTCACCCGGTCACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACGCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAc < 2:260412/137‑1 (MQ=1) caagggATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGtcc > 2:2709289/4‑138 (MQ=1) | CGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC > NZ_CP034943/2980021‑2980296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |