Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,183,714 T→G 5.4% V209V (GTT→GTG mdtP → multidrug efflux MFS transporter MdtP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,183,7140TG 5.4% 477.6 / 10.8 147V209V (GTT→GTGmdtPmultidrug efflux MFS transporter MdtP
Reads supporting (aligned to +/- strand):  ref base T (64/74);  new base G (4/4);  total (68/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 148 total aligned reads displayed.

TGGAAATGGGTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATC  >  NZ_CP034943/3183577‑3183852
                                                                                                                                         |                                                                                                                                          
tGGACAGGGGTTTTCTATTTTAACCGTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGt                                                                                                                                          <  2:2210330/140‑1 (MQ=255)
tGGAAATGGGTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGt                                                                                                                                          <  2:1424989/140‑1 (MQ=255)
   aaaTGGGTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAg                                                                                                                                       >  2:2076560/1‑140 (MQ=255)
   aaaTGGGTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAg                                                                                                                                       >  1:2596838/1‑140 (MQ=255)
       gggTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGcctc                                                                                                                                   <  1:146139/140‑1 (MQ=255)
          ttttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCtt                                                                                                                                >  1:1530244/1‑140 (MQ=255)
          ttttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTACTGCCAGAGGATTACAGGGCCGCCAGCAGAGTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGAGGATTGTCGGGGTTGTCAGCCTCCtt                                                                                                                                >  1:1076685/1‑140 (MQ=255)
           tttatctatTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCACCGTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtc                                                                                                                               <  2:561587/134‑1 (MQ=255)
            ttatataTTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                              <  2:377015/137‑1 (MQ=255)
            ttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                              <  1:2783301/140‑1 (MQ=255)
            ttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                              <  1:1219291/140‑1 (MQ=255)
               tataTTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGc                                                                                                                           >  2:171854/1‑140 (MQ=255)
                    tAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTaa                                                                                                                      >  1:2669390/1‑140 (MQ=255)
                         ttCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCttt                                                                                                                 >  2:1156011/1‑140 (MQ=255)
                          tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                <  1:2318326/140‑1 (MQ=255)
                          tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                <  1:2107301/140‑1 (MQ=255)
                          tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                <  2:2817680/140‑1 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                               >  1:794085/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                               >  1:921526/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                               >  1:1876244/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                               >  1:1001095/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCACCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                               >  1:679750/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                               >  2:2848037/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                               >  1:39115/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                               >  2:2524884/1‑140 (MQ=255)
                           ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                               >  1:2923823/1‑140 (MQ=255)
                           ccAGTCGCAATAATTGCGGTTATTTTTATTGCCAGAGGATTACAGCGCCGCCAGCAGACTGGTCCGATCAACTTCGATATAGCTGCTATTTTCACGATGATGGTCGGGGTTGTCAGACTCATTCTCGAACTAAGATTTgg                                                                                                               >  1:1753029/1‑140 (MQ=255)
                               tCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                           <  2:1324991/140‑1 (MQ=255)
                               tCGGAATGATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                           <  2:1893925/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:560164/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:1763093/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:1530901/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:2559942/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:2464783/140‑1 (MQ=255)
                                cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                          <  2:233460/140‑1 (MQ=255)
                                     ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                     <  2:2712022/140‑1 (MQ=255)
                                     ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                     <  2:2810088/140‑1 (MQ=255)
                                     ttagtGCGGTTAATTTGATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGAAGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                     <  2:2367277/140‑1 (MQ=255)
                                         tGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATg                                                                                                 >  1:3091603/1‑140 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             <  2:2116640/140‑1 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             <  1:2076560/140‑1 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             >  1:524901/1‑140 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             <  1:2379561/140‑1 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             >  1:2588070/1‑140 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             <  2:2843526/140‑1 (MQ=255)
                                             gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCCGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             >  2:2785925/1‑140 (MQ=255)
                                                   tttATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                       <  2:2173066/140‑1 (MQ=255)
                                                       ttGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATgg                                                                                   <  2:1697746/140‑1 (MQ=255)
                                                        ggCCAGAGGATTCCAGGGCCGCCAGCAGACTGGTCCGATCACCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGc                                                                                  <  1:357148/139‑1 (MQ=255)
                                                         gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                 <  2:1951994/140‑1 (MQ=255)
                                                         gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                 <  2:2596838/140‑1 (MQ=255)
                                                         gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                 <  2:908859/140‑1 (MQ=255)
                                                         gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                 <  1:171854/140‑1 (MQ=255)
                                                                   tACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGc                                                                       <  1:436933/140‑1 (MQ=255)
                                                                        ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                  <  2:2654251/140‑1 (MQ=255)
                                                                        ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                  >  1:2235155/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTCTGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:1398962/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:1833301/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:117674/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:986599/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:575750/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:1325347/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:2819159/1‑140 (MQ=255)
                                                                          ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                >  1:1940839/1‑140 (MQ=255)
                                                                          ccgccACCAGAATGGTCCGAACAAATTCGAAAAAGGTGGTATTTTAACTATAATTGTCGGGGTTGTCAGCCTCAAACTCGCCCAAACATTTTGCGGAAAATATAATGCTTGGGGTTCATGACAGATAATTGGCTttttac                                                                >  2:2978135/1‑138 (MQ=255)
                                                                           cgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTCAGCTTTGGCGGAAATGATTATGCGTGGGGTTCATGGCAGAGTCTTGGCTTGTTCGc                                                               >  1:1783722/1‑140 (MQ=255)
                                                                             ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                              <  2:2082041/75‑1 (MQ=255)
                                                                             ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                              >  1:2082041/1‑75 (MQ=255)
                                                                             ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCAc                                                             >  2:2180496/1‑140 (MQ=255)
                                                                             ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCAc                                                             >  1:1683977/1‑140 (MQ=255)
                                                                                  agACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcgcg                                                        <  2:1242692/140‑1 (MQ=255)
                                                                                        gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                  >  2:83023/1‑140 (MQ=255)
                                                                                        gTCCGATCAACTTCGATATCGCTCGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                  >  2:2154615/1‑140 (MQ=255)
                                                                                          ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                <  2:794085/140‑1 (MQ=255)
                                                                                          ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                <  2:921526/140‑1 (MQ=255)
                                                                                             aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                             >  1:2908312/1‑92 (MQ=255)
                                                                                             aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                       >  1:2119781/1‑98 (MQ=255)
                                                                                              tCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCAtt                                            >  1:2635630/1‑140 (MQ=255)
                                                                                                aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGt                                          <  2:1210340/140‑1 (MQ=255)
                                                                                                aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGGTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATGATGGGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCTCGCTGATCGGCATTGt                                          >  2:1320468/1‑140 (MQ=255)
                                                                                                 aCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTc                                         <  1:2987800/140‑1 (MQ=255)
                                                                                                  cTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCa                                        >  1:2480885/1‑140 (MQ=255)
                                                                                                  cTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCa                                        >  1:448176/1‑140 (MQ=255)
                                                                                                   ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                       <  2:1773522/140‑1 (MQ=255)
                                                                                                   ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                       <  2:679750/140‑1 (MQ=255)
                                                                                                   ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                       <  2:416521/140‑1 (MQ=255)
                                                                                                            gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcg                                                          <  1:1477398/112‑1 (MQ=255)
                                                                                                            gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcg                                                          >  2:1477398/1‑112 (MQ=255)
                                                                                                            gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTtattat                              >  2:1911407/1‑140 (MQ=255)
                                                                                                                 tATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCGTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGTGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAg                         <  1:264959/140‑1 (MQ=255)
                                                                                                                              atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa            <  2:2513016/140‑1 (MQ=255)
                                                                                                                              atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa            <  2:917665/140‑1 (MQ=255)
                                                                                                                              atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa            <  2:2962375/140‑1 (MQ=255)
                                                                                                                              atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa            <  2:2090606/140‑1 (MQ=255)
                                                                                                                               ttGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGc                                                                       <  2:2552890/80‑1 (MQ=255)
                                                                                                                                    ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                          >  2:1065690/1‑120 (MQ=255)
                                                                                                                                    ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                          <  1:1065690/120‑1 (MQ=255)
                                                                                                                                      ggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTa    <  2:1940839/140‑1 (MQ=255)
                                                                                                                                      ggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTa    <  2:884828/140‑1 (MQ=255)
                                                                                                                                      ggTAGTCACACTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTACATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTCGAATCAAAAGCAAAAGAGCCTa    <  2:774905/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TGGAAATGGGTTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATC  >  NZ_CP034943/3183577‑3183852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: