Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,183,726 T→G 5.5% L213L (CTT→CTG mdtP → multidrug efflux MFS transporter MdtP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,183,7260TG 5.5% 474.5 / 10.8 146L213L (CTT→CTGmdtPmultidrug efflux MFS transporter MdtP
Reads supporting (aligned to +/- strand):  ref base T (67/70);  new base G (4/4);  total (72/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 164 total aligned reads displayed.

TTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATC  >  NZ_CP034943/3183587‑3183865
                                                                                                                                           |                                                                                                                                           
ttttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCtt                                                                                                                                             >  1:1079787/1‑140 (MQ=255)
ttttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCtt                                                                                                                                             >  1:1530244/1‑140 (MQ=255)
 tttatctatTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCACCGTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtc                                                                                                                                            <  2:561587/134‑1 (MQ=255)
  ttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                                           <  1:2783301/140‑1 (MQ=255)
  ttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                                           <  1:1219291/140‑1 (MQ=255)
  ttCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                                           <  2:1561986/140‑1 (MQ=255)
     tataTTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGc                                                                                                                                        >  2:171854/1‑140 (MQ=255)
          tAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTaa                                                                                                                                   >  1:2669390/1‑140 (MQ=255)
               ttCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCttt                                                                                                                              >  2:1156011/1‑140 (MQ=255)
                tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                             <  1:2318326/140‑1 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                            >  1:1001095/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                            >  1:794085/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                            >  1:921526/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                            >  2:1466731/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                            >  1:1773522/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                            >  2:2524884/1‑140 (MQ=255)
                 ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                            >  1:39115/1‑140 (MQ=255)
                 ccAGTCGCAATAATTGCGGTTATTTTTATTGCCAGAGGATTACAGCGCCGCCAGCAGACTGGTCCGATCAACTTCGATATAGCTGCTATTTTCACGATGATGGTCGGGGTTGTCAGACTCATTCTCGAACTAAGATTTgg                                                                                                                            >  1:1753029/1‑140 (MQ=255)
                     tCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATCTCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                                        <  2:777275/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCATCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:2178911/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:2464783/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:2559942/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:1530901/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:873185/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:560164/140‑1 (MQ=255)
                      cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                       <  2:1762614/140‑1 (MQ=255)
                           ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                                  <  2:2712022/140‑1 (MQ=255)
                           ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                                  <  2:2810088/140‑1 (MQ=255)
                           ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTAACGATGATTGTCGGGGGTGTCAGCCTCCTTCTCGCCCTAAGCTTGGGCGGAAAAGAt                                                                                                                  <  2:3073998/140‑1 (MQ=255)
                           ttagtGCGGTTAATTTGATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGAAGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                                  <  2:2367277/140‑1 (MQ=255)
                               tGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATg                                                                                                              >  1:3091603/1‑140 (MQ=255)
                                   gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          <  2:2843526/140‑1 (MQ=255)
                                   gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          >  1:524901/1‑140 (MQ=255)
                                   gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          <  1:2076560/140‑1 (MQ=255)
                                   gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCCGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          >  2:2785925/1‑140 (MQ=255)
                                         tttATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                    <  2:2173066/140‑1 (MQ=255)
                                             ttGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATCTCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATgg                                                                                                <  2:1916901/140‑1 (MQ=255)
                                              ggCCAGAGGATTCCAGGGCCGCCAGCAGACTGGTCCGATCACCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGc                                                                                               <  1:357148/139‑1 (MQ=255)
                                               gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                              <  2:2596838/140‑1 (MQ=255)
                                               gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                              <  2:908859/140‑1 (MQ=255)
                                               gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                              <  2:908867/140‑1 (MQ=255)
                                                  agagGATTACATGGACGCCAGCACACTGGTACGATCAAATTCGATATCGCTGCTATTTTCAAGATGAATGTCTGGGGTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCAGAAAATATTATGCGTGGGGTTCATGGCAGAt                                                                                           >  2:1248375/1‑140 (MQ=255)
                                                         tACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGc                                                                                    <  1:436933/140‑1 (MQ=255)
                                                              ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                               >  1:2097026/1‑140 (MQ=255)
                                                              ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                               <  2:2654251/140‑1 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTCTGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             >  1:1398962/1‑140 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             >  1:2819159/1‑140 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             >  1:1940839/1‑140 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             >  1:1833301/1‑140 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             <  1:2978135/140‑1 (MQ=255)
                                                                ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                             >  1:1325347/1‑140 (MQ=255)
                                                                ccgccACCAGAATGGTCCGAACAAATTCGAAAAAGGTGGTATTTTAACTATAATTGTCGGGGTTGTCAGCCTCAAACTCGCCCAAACATTTTGCGGAAAATATAATGCTTGGGGTTCATGACAGATAATTGGCTttttac                                                                             >  2:2978135/1‑138 (MQ=255)
                                                                   ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                                           >  1:2082041/1‑75 (MQ=255)
                                                                   ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTtct                                                                                                                                           <  2:2082041/75‑1 (MQ=255)
                                                                              gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                               >  1:1694756/1‑140 (MQ=255)
                                                                              gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                               >  1:626112/1‑140 (MQ=255)
                                                                                ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                             <  2:794085/140‑1 (MQ=255)
                                                                                ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                             <  2:921526/140‑1 (MQ=255)
                                                                                ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                             <  2:1876244/140‑1 (MQ=255)
                                                                                ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                             <  2:1001095/140‑1 (MQ=255)
                                                                                   aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          <  2:2908312/92‑1 (MQ=255)
                                                                                   aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                          >  1:2908312/1‑92 (MQ=255)
                                                                                   aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                    >  1:2119781/1‑98 (MQ=255)
                                                                                   aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                    <  2:2119781/98‑1 (MQ=255)
                                                                                    tCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCAtt                                                         >  1:2635630/1‑140 (MQ=255)
                                                                                      aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGt                                                       <  2:1210340/140‑1 (MQ=255)
                                                                                      aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGGTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATGATGGGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCTCGCTGATCGGCATTGt                                                       >  2:1320468/1‑140 (MQ=255)
                                                                                        cTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCa                                                     >  1:2480885/1‑140 (MQ=255)
                                                                                         ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                                    <  2:679750/140‑1 (MQ=255)
                                                                                         ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                                    <  2:1773522/140‑1 (MQ=255)
                                                                                                  gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGTTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTtattat                                           >  2:2305789/1‑140 (MQ=255)
                                                                                                  gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcg                                                                       <  1:1477398/112‑1 (MQ=255)
                                                                                                  gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTtattat                                           >  2:1911407/1‑140 (MQ=255)
                                                                                                   cTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATc                                          >  1:458562/1‑140 (MQ=255)
                                                                                                       tATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCGTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGTGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAg                                      <  1:264959/140‑1 (MQ=255)
                                                                                                       gagtttCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTGGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAg                                      <  1:2208431/137‑1 (MQ=255)
                                                                                                                    atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                         <  2:2513016/140‑1 (MQ=255)
                                                                                                                    atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                         <  2:2024420/140‑1 (MQ=255)
                                                                                                                    atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                         <  2:2090606/140‑1 (MQ=255)
                                                                                                                    atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                         <  2:2962375/140‑1 (MQ=255)
                                                                                                                     ttGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGc                                                                                    >  1:2552890/1‑80 (MQ=255)
                                                                                                                        tCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAagag                     <  1:1875241/140‑1 (MQ=255)
                                                                                                                          ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                                       <  1:1065690/120‑1 (MQ=255)
                                                                                                                          ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                                       >  2:1065690/1‑120 (MQ=255)
                                                                                                                            ggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTa                 <  2:2819159/140‑1 (MQ=255)
                                                                                                                                gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTATAATCAAAAGCAAAAGAGCCTATCCt             >  2:317984/1‑140 (MQ=255)
                                                                                                                                gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt             <  2:2097026/140‑1 (MQ=255)
                                                                                                                                gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt             <  1:2936022/140‑1 (MQ=255)
                                                                                                                                gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt             >  2:1950320/1‑140 (MQ=255)
                                                                                                                                gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt             >  2:2568651/1‑140 (MQ=255)
                                                                                                                                  cAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGc           >  1:649884/1‑140 (MQ=255)
                                                                                                                                     cctcctTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCaa        <  1:1156011/140‑1 (MQ=255)
                                                                                                                                        cctTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGt     >  2:2231918/1‑140 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCTCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGAACTAGAGCCTATCCTGCCAATGTATc  >  1:915539/1‑140 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  <  2:39115/140‑1 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  >  2:2762034/1‑140 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  <  2:2305928/140‑1 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  <  2:1779919/140‑1 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  >  1:1598586/1‑140 (MQ=255)
                                                                                                                                           tctcGCCCTAAGCTGTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc  <  2:1779884/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TTTTCTATATTAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATC  >  NZ_CP034943/3183587‑3183865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: