Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,183,733 C→T 5.1% L216L (CTA→TTA)  mdtP → multidrug efflux MFS transporter MdtP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,183,7330CT 5.1% 517.1 / 11.7 156L216L (CTA→TTA) mdtPmultidrug efflux MFS transporter MdtP
Reads supporting (aligned to +/- strand):  ref base C (71/77);  new base T (4/4);  total (75/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 156 total aligned reads displayed.

TAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATCTATT  >  NZ_CP034943/3183597‑3183869
                                                                                                                                        |                                                                                                                                        
     ttCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCttt                                                                                                                                  >  2:1156011/1‑140 (MQ=255)
      tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                                 <  2:2817680/140‑1 (MQ=255)
      tCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTg                                                                                                                                 <  1:2318326/140‑1 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                                >  1:1001095/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                                >  1:921526/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGGGGATTACAGGGACGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTgg                                                                                                                                >  1:794085/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCACCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  1:679750/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  2:2524884/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  1:1486490/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  1:1773522/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  1:416521/1‑140 (MQ=255)
       ccAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTgg                                                                                                                                >  1:2923823/1‑140 (MQ=255)
       ccAGTCGCAATAATTGCGGTTATTTTTATTGCCAGAGGATTACAGCGCCGCCAGCAGACTGGTCCGATCAACTTCGATATAGCTGCTATTTTCACGATGATGGTCGGGGTTGTCAGACTCATTCTCGAACTAAGATTTgg                                                                                                                                >  1:1753029/1‑140 (MQ=255)
           tCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATCTCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                                            <  2:777275/140‑1 (MQ=255)
           tCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                                            <  2:1324991/140‑1 (MQ=255)
           tCGGAATGATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGa                                                                                                                            <  2:1893925/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCATCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:2178911/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:233460/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:560164/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:1762614/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:873185/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:2559942/140‑1 (MQ=255)
            cGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGaa                                                                                                                           <  2:2464783/140‑1 (MQ=255)
                 ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                                      <  2:2810088/140‑1 (MQ=255)
                 ttattGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGAt                                                                                                                      <  2:2712022/140‑1 (MQ=255)
                     tGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATg                                                                                                                  >  1:3091686/1‑140 (MQ=255)
                         gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                              <  2:2843526/140‑1 (MQ=255)
                         gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                              >  1:276361/1‑140 (MQ=255)
                         gTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCCGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                              >  2:2785925/1‑140 (MQ=255)
                               tttATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                        <  2:2701218/140‑1 (MQ=255)
                                   ttGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATgg                                                                                                    <  2:1697746/140‑1 (MQ=255)
                                    ggCCAGAGGATTCCAGGGCCGCCAGCAGACTGGTCCGATCACCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGc                                                                                                   <  1:357148/139‑1 (MQ=255)
                                     gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                                  <  2:2596838/140‑1 (MQ=255)
                                     gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                                  <  2:1951994/140‑1 (MQ=255)
                                     gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                                  <  2:908859/140‑1 (MQ=255)
                                     gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                                  <  2:2569726/140‑1 (MQ=255)
                                     gCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCa                                                                                                  <  2:908867/140‑1 (MQ=255)
                                        agagGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGAt                                                                                               >  2:2393500/1‑140 (MQ=255)
                                                    ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                                   >  1:2097026/1‑140 (MQ=255)
                                                    ggCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGtt                                                                                   >  1:2235155/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTCTGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:1398962/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:575750/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:1833301/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 <  1:2978135/140‑1 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:2819159/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:1940839/1‑140 (MQ=255)
                                                      ccgccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCg                                                                                 >  1:117674/1‑140 (MQ=255)
                                                      ccgccACCAGAATGGTCCGAACAAATTCGAAAAAGGTGGTATTTTAACTATAATTGTCGGGGTTGTCAGCCTCAAACTCGCCCAAACATTTTGCGGAAAATATAATGCTTGGGGTTCATGACAGATAATTGGCTttttac                                                                                 >  2:2978135/1‑138 (MQ=255)
                                                         ccAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCAc                                                                              >  2:2180496/1‑140 (MQ=255)
                                                              agACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcgcg                                                                         <  2:1242692/140‑1 (MQ=255)
                                                                    gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                                   >  1:1694756/1‑140 (MQ=255)
                                                                    gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                                   >  1:626112/1‑140 (MQ=255)
                                                                    gTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                                   >  2:83023/1‑140 (MQ=255)
                                                                    gTCCGATCAACTTCGATATCGCTCGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATc                                                                   >  2:2154615/1‑140 (MQ=255)
                                                                      ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                                 <  2:921526/140‑1 (MQ=255)
                                                                      ccGATCAACTTCGATATCGCTGGTATTTTCACAATGATTGTCGGTGTGGTCAGCCTCCTGCTCGCCTTAAGCTTTGGCGGAAAAGATTATGCATGGGATTCATGGCAGATTCTTGGGTTGTTCGCTCTCGCACTGATCgg                                                                 <  2:1001095/140‑1 (MQ=255)
                                                                         aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                              >  1:2908312/1‑92 (MQ=255)
                                                                         aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTg                                                                                                              <  2:2908312/92‑1 (MQ=255)
                                                                         aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                        >  1:2119781/1‑98 (MQ=255)
                                                                         aTCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTc                                                                                                        <  2:2119781/98‑1 (MQ=255)
                                                                          tCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCAtt                                                             >  1:2635630/1‑140 (MQ=255)
                                                                          tCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCAtt                                                             >  1:1406025/1‑140 (MQ=255)
                                                                            aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGt                                                           <  2:1210340/140‑1 (MQ=255)
                                                                            aaCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGGTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATGATGGGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCTCGCTGATCGGCATTGt                                                           >  2:1320468/1‑140 (MQ=255)
                                                                             aCTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTc                                                          <  1:2987800/140‑1 (MQ=255)
                                                                              cTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCa                                                         >  1:448176/1‑140 (MQ=255)
                                                                              cTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCa                                                         >  1:2480885/1‑140 (MQ=255)
                                                                               ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                                        <  2:679750/140‑1 (MQ=255)
                                                                               ttCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAg                                                        <  2:1773522/140‑1 (MQ=255)
                                                                                       cGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTttatta                                                >  1:1255817/1‑140 (MQ=255)
                                                                                        gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTcg                                                                           >  2:1477398/1‑112 (MQ=255)
                                                                                        gCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTtattat                                               >  2:1911407/1‑140 (MQ=255)
                                                                                         cTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATc                                              >  1:458562/1‑140 (MQ=255)
                                                                                             tATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCGTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGTGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAg                                          <  1:264959/140‑1 (MQ=255)
                                                                                                          atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                             <  2:2024420/140‑1 (MQ=255)
                                                                                                          atTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                             <  2:2090606/140‑1 (MQ=255)
                                                                                                          agtGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCCTGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATcaaaagcaaa                             <  2:2523805/138‑1 (MQ=255)
                                                                                                           ttGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGc                                                                                        >  1:2552890/1‑80 (MQ=255)
                                                                                                              tCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAagag                         <  1:1875241/140‑1 (MQ=255)
                                                                                                                ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                                           <  1:1065690/120‑1 (MQ=255)
                                                                                                                ggggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTa                                           >  2:1065690/1‑120 (MQ=255)
                                                                                                                  ggTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTa                     <  2:1940839/140‑1 (MQ=255)
                                                                                                                  ggTAGTCACACTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTACATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTCGAATCAAAAGCAAAAGAGCCTa                     <  2:774905/140‑1 (MQ=255)
                                                                                                                    ttGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATc                   >  1:1965087/1‑140 (MQ=255)
                                                                                                                      gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTATAATCAAAAGCAAAAGAGCCTATCCt                 >  2:317984/1‑140 (MQ=255)
                                                                                                                      gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt                 <  2:2235155/140‑1 (MQ=255)
                                                                                                                      gTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCt                 <  2:2097026/140‑1 (MQ=255)
                                                                                                                           cctcctTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCaa            <  1:2089200/140‑1 (MQ=255)
                                                                                                                              cctTCTCGCCCTAAGCTTTGGTGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGt         >  2:177794/1‑140 (MQ=255)
                                                                                                                              cctTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGt         >  2:1790842/1‑140 (MQ=255)
                                                                                                                              cctTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGt         >  2:2983990/1‑140 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCTCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGAACTAGAGCCTATCCTGCCAATGTATc      >  1:915539/1‑140 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      <  2:1779919/140‑1 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      >  1:1598586/1‑140 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      >  2:2762034/1‑140 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      <  2:39115/140‑1 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      <  2:2923823/140‑1 (MQ=255)
                                                                                                                                 tctcGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATc      <  2:2305928/140‑1 (MQ=255)
                                                                                                                                   tcGCCCTAAGCGTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATCTa    <  2:502594/140‑1 (MQ=255)
                                                                                                                                     gCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATCTAtt  >  1:2588463/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TAACCTTCCAGTCGGAATTATTGCGGTTATTTTTATTGCCAGAGGATTACAGGGCCGCCAGCAGACTGGTCCGATCAACTTCGATATCGCTGGTATTTTCACGATGATTGTCGGGGTTGTCAGCCTCCTTCTCGCCCTAAGCTTTGGCGGAAAAGATTATGCGTGGGGTTCATGGCAGATTCTTGGCTTGTTCGCACTCGCGCTGATCGGCATTGTCAGCTTTATTATCGTAGAATCAAAAGCAAAAGAGCCTATCCTGCCAATGTATCTATT  >  NZ_CP034943/3183597‑3183869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: