Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,137 T→A 16.2% I50N (ATC→AAC)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1370TA16.2% 395.7 / 11.0 179I50N (ATC→AAC) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (79/61);  new base A (3/20);  total (82/97)
Fisher's exact test for biased strand distribution p-value = 9.16e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

CGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAG  >  NZ_CP034943/548999‑549275
                                                                                                                                          |                                                                                                                                          
cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                                           >  2:434543/1‑140 (MQ=255)
cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                                           >  1:2683221/1‑140 (MQ=255)
cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                                           <  1:2261254/140‑1 (MQ=255)
 gCCTTTTTTGAATATAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGAATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCATGGCGAGTCAGCTTTCCATCa                                                                                                                                          >  2:397865/1‑140 (MQ=255)
 gCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCa                                                                                                                                          >  2:1239892/1‑140 (MQ=255)
       ttttAATAAAAATCTGTATATTCCGATTTATCAGCAGCTTTATCGCTTTTACAAACAAAACATCAATCAGGGGCTTATTCAAAAAGTAATTAAGCGCCCTTCAAAAACACTCGTGGCGAGTCAGCTTTCCATCAGTaaaa                                                                                                                                    <  2:2535823/140‑1 (MQ=255)
       tttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACAGGCATCCGGGGCGTATTCAAAAAGGATTGAAGCGCCCTTCAAAAACACTCCGGGCGAGTCAGCTTTCCACCAGTCaca                                                                                                                                    <  2:551814/140‑3 (MQ=255)
       tttCAATAGAAATCTGTATATTCCGCTTTATAAGCATATTTTTCGCTATTTAAAACAAAACATCAATCAGGGGGGTATTCAAATAGGAATTAAGCGCCCTTCCAAAAGACTCCTGGCGTGTCAGCTCTCAATCAGTCaaa                                                                                                                                    <  2:2065583/140‑1 (MQ=255)
             tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                                                                              >  1:1566300/1‑140 (MQ=255)
                aaaTCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGTGTCAGCTTTCCATCAGTCAAACGACTGTAg                                                                                                                           >  2:2328138/1‑140 (MQ=255)
                  aTCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGaa                                                                                                                         >  2:2240354/1‑140 (MQ=255)
                    ttgtatatTCCGCTTTATCAGCATGTTTATCGCTATTTCAAATAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTGCCAAACGCCTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                       <  2:81355/136‑1 (MQ=255)
                    gtgcatatTCCTCTTTATCAGCAGATTTAGCGATATTTCAAATAAAACATGCATCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCAAAAACACGCGTGGCGAGTCAGCTTCCCAACAGTCAAACGACTGTAGAACg                                                                                                                       <  2:524621/136‑1 (MQ=255)
                    cTGTATATTCCGCTTTATCAGAAGCTTTATGGCTATTTCAAAGAAAACTTGCATCTGGGGCTTATTAAAAATGTAATTAAGCTCCCTTCAAAAAGACTCGTGGCGAGTAAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                       <  2:326026/140‑1 (MQ=255)
                    cTGTATATTCCGCTTTATAAGCAGCTTTATCGCTTTTTAAAACAAAACATGCATCCGGGGGTTATTAAAAACGGAATTAAGCTCCCTTCAAAAAGCCTCCTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACg                                                                                                                       <  2:972979/140‑1 (MQ=255)
                    cTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAACAAAACTTGCATCCGGGGCGTATTCAAAAAGTATTGAAGCTCCCTGCCAAAAGACTCCTGGGGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                       <  2:705455/140‑1 (MQ=255)
                    cTGGATATACCTCTTTATCAGCCGCTTTCTCGCTATTTCAAATAAAACATCAATCCGGGGCTTATAAAAAAAGGAATGAAGCTCCCTACAAAAACACTCCTGCCGACTCAGCTTTCCATCAGACACACGACTGTAGAACg                                                                                                                       <  1:3060050/140‑1 (MQ=255)
                     tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGt                                                                                                                      >  1:2607965/1‑140 (MQ=255)
                       gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCCTCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                                    >  1:3000783/1‑140 (MQ=255)
                       gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                                    >  1:568788/1‑140 (MQ=255)
                       gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                                    >  1:569324/1‑140 (MQ=255)
                       gATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGc                                                                                                                    >  1:495437/1‑140 (MQ=255)
                           ttCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTAt                                                                                                                >  2:2968068/1‑140 (MQ=255)
                           ttCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTAt                                                                                                                >  2:2432363/1‑140 (MQ=255)
                            tactcTTTATCAGCAGCTTTATATATAATACAAAGAAAACATGAATAAGAGGCGTATTAAAAAATGAATTAAGCTACATTCCAAAATACTCCTGGCGATTCAGATTTACAACAGTCAAACGACTGTAGAACGTGACTAtt                                                                                                               >  2:2708331/5‑139 (MQ=255)
                            tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAATAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCACTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                                               >  1:1419026/1‑140 (MQ=255)
                            tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                                               >  1:1911690/1‑140 (MQ=255)
                              cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATAAGTCAAACGACTGTAGAACTTGCCTATTaa                                                                                                             >  1:1011327/1‑140 (MQ=255)
                                  ttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGc                                                                                                         >  1:2376907/1‑140 (MQ=255)
                                  ttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAACGAATGAAGCTCCATTCCAAAAGACTCCTGTCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAAAAga                                                                                                         >  1:2383165/1‑139 (MQ=255)
                                     tCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAGTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                      >  2:2677578/1‑140 (MQ=255)
                                     tCACCAGCTTTAGCGCTATTACACACAAAACATGAATCCGGGGCTTATTCAAAACGGAATTAAGCCCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCATCCGACACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                      <  1:2567208/140‑1 (MQ=255)
                                     cccgcagcTTTATCGATATTTCAACTAAAACCTGCATCCGGGGCGTATAAAAAAAGGAATGAAGCTCCCGTCAAACCCACTCCGGGCGAGGCAGCTTTCCATCACTAACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                      <  2:1216906/137‑1 (MQ=255)
                                     acagcagCTTTATCGCTATTTAAAAGAAAACATGCATCCGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                      <  2:1157254/139‑1 (MQ=255)
                                     acagcagCTTTATCGCTATTAAAAACAAAACATCCATCCGGGGCGTATTAAAAAAGCAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTACCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                      <  2:562722/139‑1 (MQ=255)
                                        ccagtTTTATCGCTATTCAAAACAAAACACCCAACCGGGGCGTATTCAAAAAGGAATGACGCTCCCTACAAAACCACCCCTGGCGAGTAAGCTTTCCAACACTAAAACGACTGTAGAACGTGCCTATGAACAGCTtgctg                                                                                                   <  1:551157/139‑1 (MQ=255)
                                          cgcTTTATCGCTATTACAAACAAAACCTGCATCCGGGGCGTATTCAAAAAGCAATCACGCGCCCTACCAAACCACGCCGGGCCAGTCAGCTTCCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:2827284/139‑1 (MQ=255)
                                          cgcTTAATCGCTATTACAAACAAAACATGCAACCGGGGCGAATTAAAAACCTAATGAAGCGCCCTACCACACCCCACCTGGCGAGTCCGCTTACCAACACTAAAACGACTGTAGAACCTGCCTATGAACAGCTTgctgct                                                                                                 <  1:180830/139‑1 (MQ=255)
                                          agttttATCGATATTTCAAACAAAACATCCATCCGGGGCTTATCAAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCGGGCTATTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:1930338/137‑1 (MQ=255)
                                          aggtttACCGCTATTTCAAACAAAACATCAATCCGGGGCGTATTCAAAAAGGACTCAAGCACCGTTCCAAACGACGCCTGGCGTTTCAGTGTCCCCTCAGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:530323/137‑1 (MQ=255)
                                          agcTTTATCTATATTAAAAACAAAACCTGCATCCGGGGCTTATTCAAAAAGGAATGAACCCCCCTTCCAAACCACCCCGGGCGACCCAGCTTACCACCACACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:1327955/140‑1 (MQ=255)
                                          agcTTTATCGTTATTTAAAAGAAACCTTCAATCCGGGGCGTAGTCAAAAAGTAAGGATGCGCCATACCAAAAGACTCCGGGCGAGTCAGTTTTCCATCAGTCAAACGACTGTAGAACGTGCCTCTGAACAGCTTgctgct                                                                                                 <  2:1998616/140‑1 (MQ=255)
                                          agcTTTATCGCTATTACAAACAAAACATGACTCAGGGGCTTTTTAAAAAAGGAATGAAGCGCCCTTCCAAAACACGCCGGGCGAGTCAGCTTGCCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  2:990889/140‑1 (MQ=255)
                                          agcTTTATCGATATTTAAAATAAACCATGCATCTGGGGCTTATAAAAAAAGGAATGAAGCTCCCTTCAAAAACACCCCTGGCGAGTCAGCTTACCATCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:92532/140‑1 (MQ=255)
                                          agcTTTATCGATATTTAAAACAAAACATGAATCTGGGGCTTATTCAAAAAGCAATGAAGCGCCCTCCAAAAAGACACCTGGCCAGTCAGCTTACCAACACTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:2808485/140‑1 (MQ=255)
                                          agcTTTATCGATATTACAAACAAAACATCCATCCGGGGCGTATACAAAAAGCACTCACGCCCCCTACCAAAAGACTCCTGGCGAGTCAGGTTGCCCTCCGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:6275/140‑1 (MQ=255)
                                          agcTTTAGCGATATTACAAACAAAACCTGCATCCGGGGCGTATCCAAAAAGGATTGAAGCTCCCTTCCAAAAGACCCCGGGCGAGAAAGCTTACCATCACACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:180397/140‑1 (MQ=255)
                                          agcTTTAGCGATATTACAAACAAAACATGCAACCGGGGCGTATTCAAAACGCAATGACGCGCCCTGCCAAACGCCCCGGGGCGAGTCAGGTTTCAACCCGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:1819404/140‑1 (MQ=255)
                                          agcTTTACCGCTTTTTCAAACAAACCATGCATCAGGGCCGTATAAAAAAAGCAATGAAGCGCCCTACCAAAACCCGCGGGGCGAGTCTGCTTTCCATCCGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:1082564/140‑1 (MQ=255)
                                          agcTTTACCGATATTTCAAACAAAACATGAATCCGGGGCGTAAACAAAAAGGATTGACGCTCCCTACCAAAACACTCCGGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:744571/140‑1 (MQ=255)
                                          agcTTACTCGCTATTACAAAGAAAACATGCATCAGGGGCGTATTAAAAACGGAATGAAGCTCCCTTCCAAAACACCCGGGGCGAGTCAGCTTGCCAGCACTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                 <  1:2382819/140‑1 (MQ=255)
                                             tttATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAAAAGCTTGCTGCTGAg                                                                                              >  2:2891731/1‑140 (MQ=255)
                                                 tCGCTATTCAAAACAAAACAGGCATCCGGGGCGTATAAAAAAAGGATTGACGCTCCCTACCAAACCACGCCGGGCGAGTCAGCTTCCCAACCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                                          <  1:402211/140‑1 (MQ=255)
                                                 tCGATATTTCAAACAAAACATCCAACCGGGGGTTATTAAAAAAGGAATTAAGCTCCCTTCCAAACGCCCCCGGGCGAGTCAGCTTTCCACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                                          <  2:866462/140‑1 (MQ=255)
                                                      aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     >  1:1380501/1‑140 (MQ=255)
                                                      aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     >  2:141041/1‑140 (MQ=255)
                                                      aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     >  2:295878/1‑140 (MQ=255)
                                                      aTTTCAAAGAAAACATGCAACAGGGGCTTATTAAAAAAGGAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCAATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     <  2:1071237/140‑1 (MQ=255)
                                                      aTTTCAAACAAAAAATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTTCCAAAACACACCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     <  2:627659/140‑1 (MQ=255)
                                                      aTTTAAAAGAAAACATGCATCTGGGGCTTATTCAAAAAGGAATGAAGATCCCTTCAAAAAGACTCCTGGCGAGTCAGCTTTCCATCCGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                     <  2:1699660/140‑1 (MQ=255)
                                                            aacaaaaCATGCATCAGGGGCTTATTAAAAAAGTATTGAAGCTCCCTTCCAAAACCCTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                                               <  1:1065136/137‑1 (MQ=255)
                                                            aacaaaaCAGCCATCAGGGGCTTATAAAAAAAGCATTGAACCCCCCTTCCAAACCACTCCTGGCCAGTCAGATTACAACCACCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                                               <  1:1010236/137‑1 (MQ=255)
                                                             ataaaaCATGCATCAGGGGGTTATTCAAAAAGTAATGAAGCTCCCTTCAAAAACCCTCCTGGCGAGTCAGATTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                                              <  2:1089519/138‑1 (MQ=255)
                                                             aGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                                              >  2:1059231/1‑140 (MQ=255)
                                                             aGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                                              >  2:1059429/1‑140 (MQ=255)
                                                             aGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                                              >  2:2408524/1‑140 (MQ=255)
                                                                 aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACTTGCCTATGAACAGCTTGATGCTGAGGGCTATATCGTGAGCAAGcc                                                                          >  2:632734/1‑140 (MQ=255)
                                                                 aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                                                                          >  2:2190624/1‑140 (MQ=255)
                                                                   aatgaaacAGGGGCTTATTCAAAACGCAATCAAGATCCCTTCCAAAACAATCCTGGCGAGTCAGATTTCCATCATAAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa                                                                        <  2:351497/133‑1 (MQ=255)
                                                                        aTCCGGGCCGAATACAAAAAGGACTGACGCTCCCTGCCAAACCCCGCCGGCCTAGTCACCTAACCACCAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGc                                                                   <  1:2801990/140‑1 (MQ=255)
                                                                                cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt                                                           >  1:2780478/1‑140 (MQ=255)
                                                                                  tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCACGCCAAGAAGCGGATGGTTTg                                                         >  2:2955164/1‑140 (MQ=255)
                                                                                  tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                         >  1:2562284/1‑140 (MQ=255)
                                                                                  tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                         >  1:1999692/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                  >  2:878227/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                  >  2:834498/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                  >  1:495213/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                  >  1:173574/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                  >  2:3086225/1‑140 (MQ=255)
                                                                                         aaaGGAATGAAGCTCCCCTCCAAAAGACTCCTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCCAGCCAAGAAGCGGATGGTTTGCGGATTa                                                  >  2:1491522/1‑140 (MQ=255)
                                                                                                        ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                                   >  1:249640/1‑140 (MQ=255)
                                                                                                            ccAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg                               <  2:495437/140‑1 (MQ=255)
                                                                                                            caaaaaGACTCCGGGCGAGTCAGTTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGACGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg                               <  1:2968068/138‑1 (MQ=255)
                                                                                                            acAAAAGACTCCTGGCTTGTCTTGTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg                               <  2:569324/139‑1 (MQ=255)
                                                                                                                    cTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaa                       >  2:1331274/1‑140 (MQ=255)
                                                                                                                      ccTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaaa                     >  2:1942517/1‑140 (MQ=255)
                                                                                                                       cTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                                               >  2:93649/1‑81 (MQ=255)
                                                                                                                          cccaGTCAGCTTTCACACCGACACACGTCTGTAGAACCTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATAcc                 <  2:540004/137‑1 (MQ=255)
                                                                                                                             aGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAg              >  1:2061337/1‑140 (MQ=255)
                                                                                                                             aGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAg              >  1:326470/1‑140 (MQ=255)
                                                                                                                               tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAtttt                                  >  1:188814/1‑118 (MQ=255)
                                                                                                                               tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAtttt                                  <  2:188814/118‑1 (MQ=255)
                                                                                                                               tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgcg            >  1:2461855/1‑140 (MQ=255)
                                                                                                                                 aGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAGTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCa          >  1:343502/1‑140 (MQ=255)
                                                                                                                                   cTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATAGCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATGTTGCGTATCGTAAAATACCAAGCGCATc        >  2:1501609/1‑140 (MQ=255)
                                                                                                                                     ttCCATTCGTCAAACGACTGTCGAACGTGCCTATGAACAGCTTGCTGCTGAGGGATATATCGTGAGCATGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTTCTTTTGCGTATCGTAAAATACCAAGCGGATCag      <  2:185982/140‑2 (MQ=255)
                                                                                                                                     ttCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCACAGAAGCGGATGGTTTCCTGAGTATCATGATTCTGATTTTGCGTATCGGAAAATACCAAGCGCATCAc      >  2:1393213/1‑140 (MQ=255)
                                                                                                                                         aTCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAg  >  1:1714637/1‑140 (MQ=255)
                                                                                                                                         aTCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAg  >  2:2583355/1‑140 (MQ=255)
                                                                                                                                         aTCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGAGTATCGTAAAATAACAAGCGCATCACCAAg  >  2:2583345/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAG  >  NZ_CP034943/548999‑549275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: