Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,193,459 C→G 8.0% E56Q (GAA→CAA)  htrB ← serine protease Do‑like protein HtrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,193,4590CG 8.0% 348.3 / 4.0 148E56Q (GAA→CAA) htrBserine protease Do‑like protein HtrB
Reads supporting (aligned to +/- strand):  ref base C (57/69);  new base G (13/2);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 2.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 148 total aligned reads displayed.

CCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGTTTGATGTTGA  >  NZ_CP034943/3193321‑3193595
                                                                                                                                          |                                                                                                                                        
ccTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTCTTTTACATTTCt                                                                                                                                         >  1:3107618/1‑140 (MQ=255)
     ttttGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCTTTTACATTTCTAAAGc                                                                                                                                    >  1:830650/1‑140 (MQ=255)
      tttGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTTTTTTTTAAAATAAGTTTCTGTCTTTTCCATTTGTatttca                                                                                                                                   <  1:1307522/140‑6 (MQ=255)
      tttGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTAATATAAGTTTCTGTCTTTTCCATTTGTatttca                                                                                                                                   <  1:970488/140‑6 (MQ=255)
          gTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAg                                                                                                                               <  2:279302/140‑1 (MQ=255)
               aTGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGtt                                                                                                                          >  2:2965812/1‑140 (MQ=255)
               aTGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGtt                                                                                                                          >  2:2477269/1‑140 (MQ=255)
                    aGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCGTTTCCATTTGTAAAGCAGAAGCCGgttaaat                                                                                                                     >  1:1753096/1‑136 (MQ=255)
                     gATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTGTCACAGCCGTGTGGGTCTTTTCCATTTCTAAAGCAGAAGCCGTTGGATGt                                                                                                                    >  1:386192/1‑140 (MQ=255)
                         aGGCGCGATGCCGAGCATCAGACGCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGGTTGTTGTCTTTTTTCACAGCGGTGTGTGTCTGTTCCAGTGCGAAACCCGAAGCCGGTTCCTggtgtc                                                                                                                >  1:3061564/1‑135 (MQ=255)
                         aGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGTAGCCGTTTCATGTTTTc                                                                                                                >  2:2833577/1‑140 (MQ=255)
                         aGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTc                                                                                                                >  2:2460364/1‑140 (MQ=255)
                         aGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTGTTTTCCATTTCTAAAGCAGAAGCCGGTTCATGTTTTc                                                                                                                >  1:373788/1‑140 (MQ=255)
                          ggCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTcc                                                                                                               <  1:481939/140‑1 (MQ=255)
                           gcgcGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGGTTCGGTGTTTTCCATTTAGAAAGCAGAAGCCGGGTCATGTTTTccc                                                                                                              >  1:342990/1‑140 (MQ=255)
                             gcgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTGTTTTCTTTTTTCACAGCCGTGTGTGTCGTTTCCATTTGTGAATGAGAATCCGTTTCCTGTTTTCCCGc                                                                                                            >  1:268220/1‑140 (MQ=255)
                              cgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTGTTTTCCATTTCTAAAGCAGAAGCCGGTTCATGTTTTGCCGCt                                                                                                           >  1:203596/1‑140 (MQ=255)
                              cgATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCt                                                                                                           <  1:1226287/140‑1 (MQ=255)
                                aTGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCGGTCTTTTCCATTTCTAAAGCAGAAGGCGTTTCATGTTTTCCCGCTTc                                                                                                         >  2:348178/1‑140 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGGCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  2:1613328/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  2:1382012/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  1:2178559/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  1:1931398/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  2:2094529/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  1:93964/140‑1 (MQ=255)
                                   ccGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATa                                                                                                      <  2:444347/140‑1 (MQ=255)
                                      aGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTGCATTTGTAAAGCAGAAGCCGGTTGATGTTTTCCCGCTTCATACGa                                                                                                   >  1:1968892/1‑140 (MQ=255)
                                      aGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGa                                                                                                   >  1:2084183/1‑140 (MQ=255)
                                      aGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTAATGTTTTCCCGCTTCATACGa                                                                                                   >  1:553293/1‑140 (MQ=255)
                                           cAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAg                                                                                              >  2:1296046/1‑140 (MQ=255)
                                           cAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGTGTGTTTTCCATTTGTAAAGCAGAAGCCGTTTGATGTTTTGCCGCGTCATACGAAAAAg                                                                                              >  1:1000935/1‑140 (MQ=255)
                                           cAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCGCCGGGTGTTTTCTTTTTTCACAGCGGTGTGTGTGTGTTCGAGGTGTAAAGCAGAAGCCGTTGGATGTTTTCACGCTTCATAAGAACCac                                                                                              >  1:809277/1‑139 (MQ=255)
                                            aGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAgg                                                                                             <  1:1892105/140‑1 (MQ=255)
                                             gACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCAATTCTAAAGCAGAAGCCGGTTCATGTTTTCCCGCTTCATACCAAACAGgc                                                                                            >  2:562248/1‑140 (MQ=255)
                                                  ccgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATACGAAACCGGCGCGTc                                                                                       >  2:2995633/1‑140 (MQ=255)
                                                   cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTGCATTTGTAAAGCAGAAGCCGGTTGATTTTTTGCCGCTTCAAACGAAACAGGCGCGTCa                                                                                      >  1:93127/1‑140 (MQ=255)
                                                   cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGGGTGGTTTGCCTTTCTAAAGCAGTAGCCGGTTCATGTTTTGCCGCTTCATTCGAAACAGGCGCGTCa                                                                                      >  1:2712428/1‑140 (MQ=255)
                                                   cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCa                                                                                      >  2:729697/1‑140 (MQ=255)
                                                   cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCTTTTGCATTTCTAAAGCTCAAGCCGTTTGATGTTTTGCCGCTTCAGACGAAACAGGCGGGTCa                                                                                      >  1:2335290/1‑140 (MQ=255)
                                                   cgccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGGGTGTGTGTTTTCCAATTGTAAAGCAGAAGCCGGTTCATGTTTTTCCGCGTCATACGAAACCGGCGCGtga                                                                                      >  1:466806/1‑138 (MQ=255)
                                                     ccgATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGTGTCTTTTGCATTTGTAAAGCAGAAGCCGGTTGATTGTTTGCCGCGTCATACGAAACAGGcgcggccag                                                                                    >  1:2346081/1‑135 (MQ=255)
                                                        aTAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTGTCACAGCCGTTTCGGTCTTTTCCATGTCTAAAGCAGAAGCCGTTTCATGTTTTCACGCTTCCTACGAAAAAGGCGCGgcaagcaa                                                                                 >  2:1502834/1‑140 (MQ=255)
                                                           aTCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAAttt                                                                              <  2:140204/140‑1 (MQ=255)
                                                            tCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTGCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATACGAAAAAGGCGAGTAAAGAAAttta                                                                             >  2:1494863/1‑139 (MQ=255)
                                                            tCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATAAGAAACAGGCGCCTCAAGCACTTTc                                                                             >  2:823517/1‑140 (MQ=255)
                                                            tCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCGGGCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATACGAAACAGGCGCGTCAAGCAATTTc                                                                             >  2:1494714/1‑140 (MQ=255)
                                                            tCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTCTTTTGCATTTATAAAGCACAACCCCTTTCAGGTTTTCCCGCGTCATACGAAACATGCGCGGCAAGCacattg                                                                             >  2:819771/1‑135 (MQ=255)
                                                              ccGCCTAAACTCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTCCATACGAAACAGGCGCGTCAAGCAATTTCTg                                                                           <  2:1531827/140‑1 (MQ=255)
                                                                gCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGtt                                                                         <  1:2289636/140‑1 (MQ=255)
                                                                gCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATAAGAAACAGGCCCGGCCAGCAAATTCtttt                                                                         >  2:1171697/1‑140 (MQ=255)
                                                                    aaaaTCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGt                                                                     <  1:1056680/140‑1 (MQ=255)
                                                                                aGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAATCACTATCCGATTCATTTTTTCACGCTTCATCCGAAACAGGCCCGTAAAGCAATTTCTGTTCAGTATGTTCAATCgg                                                         >  2:1120243/1‑140 (MQ=255)
                                                                                aGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCgg                                                         >  2:425441/1‑140 (MQ=255)
                                                                                 gCCATGCGGCTGTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTGTTTTGCATTTCTAAAGCAGAAGGCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAAGTTCTTTTCAGTATTTTCAATAGGc                                                        >  2:2304474/1‑140 (MQ=255)
                                                                                     tGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTCCATTTCTAAAGCAGAATCCGTTTGATTTTTTGCCGCGTCATACGAAACAGGCGCGTCAAGCAATTTGTTTTGATTATGTTGAATAGGCTGcc                                                    >  1:1408519/1‑140 (MQ=255)
                                                                                      gCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTATGTCTTTTCCATTTCTAAAGCAGAAACCGTTTCATTTTTTACCGCTTCAAAAGAAACAGGCGCGTCAAGCAATTTCTTTTCAGTATTTTCAATAGGCTGCCg                                                   >  1:1460118/1‑140 (MQ=255)
                                                                                      gCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTGTGGGTCGTTTGCAATTGTAAATCAGAATCCGTTTGATTTTTTCCCGCTTCATAAGAAACACGCGCGTCAAGCCATTTATGTTGAGTATTTTAAATCTGCTGCCg                                                   >  1:1949321/1‑140 (MQ=255)
                                                                                        ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                                 <  1:1296046/140‑1 (MQ=255)
                                                                                        ggCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGt                                                 <  1:2560265/140‑1 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTGCATTTGTAAAGCAGAAACCGGTTCATGTTTTGCCGCGTCATACCAAAAAGGCGCGTCAAGCAAATTCTGTTGAGTATGTTAAAAAGGCTGGCgggtt                                               >  1:153135/1‑137 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGTTTTGCATTTCTAAAGAAGAAGACGTTTCATTTTTTTCCGCTTCATACGAAACAGGCGCGTGAATCAAATTTTGTTTATTATTTTCAATAGGCTGCGGGGTa                                               >  1:2673028/1‑140 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTTTGTGTGGTTTGCATTTCTAAAGCACAAGCCGTTTGATGTTTTACCGCTTCATAACAAACAAGCGCGTCAAGCAATTTGTGTTGAGTATGTTAAAACGGCTGGCGGGTa                                               >  1:18492/1‑140 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCa                                                                                      >  2:363286/1‑101 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCa                                                                                      <  1:363286/101‑1 (MQ=255)
                                                                                          ctctCCGTTTCTTTTCTTTTTTCACAGCCGTGTCTGTATTTTCCATTTGAAAAGCAGAATCCGTTTCATGTTTTGCCTCGTCATAAGAAACAGGCGCGTCAAGCAATGTATGTTGAGGTTGTTAAATCGGCTGGCGGGTa                                               >  1:1803920/1‑140 (MQ=255)
                                                                                           tctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAt                                              <  1:1676074/140‑1 (MQ=255)
                                                                                           tctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAt                                              <  2:2556758/140‑1 (MQ=255)
                                                                                           tctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTAt                                              <  1:896993/140‑1 (MQ=255)
                                                                                            ctcCGTTTCTTTTCTTTTTTCACAGCCGTTTCGGTCTTTTCCATTTATAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTAAAAAGAAACAGGCGCGTCAAACAAATTCTGTTCAGTATGTTAAAACGGCTGGCGGTTAtt                                             >  2:8250/1‑140 (MQ=255)
                                                                                                ggttcttttcttTTGTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  2:2768069/138‑1 (MQ=255)
                                                                                                gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGTAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGTGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  1:1211262/140‑1 (MQ=255)
                                                                                                gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  1:538240/140‑1 (MQ=255)
                                                                                                gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  2:100901/140‑1 (MQ=255)
                                                                                                gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  2:2439894/140‑1 (MQ=255)
                                                                                                gTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTa                                         <  1:2460364/140‑1 (MQ=255)
                                                                                                     ttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTg                                    <  1:2903327/140‑1 (MQ=255)
                                                                                                     ttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTACCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTg                                    <  1:2995633/140‑1 (MQ=255)
                                                                                                     ttttcttttTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTGTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTg                                    <  1:1976742/140‑1 (MQ=255)
                                                                                                       ttcttttTTCACAGCCGTTTGTGTGTTTTACAATTCTAAACCACAAGCCGTTTAATGTTTTCCCGCTTCAAAAGAAACACGCGCGTCAACCAATTTCTGTTGAGTATGTTCAATCGGCGGCCGGTTATTTTTATTGTGAt                                  >  2:296066/1‑140 (MQ=255)
                                                                                                       ttcttttTTCACAGCCGTTTCTGTCGTTTAAATTTATAAAGCACAAGCCGTTTAATGTTTTCCCGCTTCATACTAAACAGGCCAGTAAAGCAATTTATGTTCAGTATTTTCAATAGGATTCCGGTTAATTGTAATATGAt                                  >  2:190794/1‑140 (MQ=255)
                                                                                                       ttcttttTTCACAGCCGTTTCTGTATTTTCCATTTCTAAAACAGAATCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCCCGTCAAGCAAATTATGTTCAGTATGTTAAAACGGCTGCCGGTTAATTTTATTCTGAt                                  >  2:1744932/1‑140 (MQ=255)
                                                                                                          ttttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGtt                               <  1:1453483/140‑1 (MQ=255)
                                                                                                           tttttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTc                              <  2:2106890/140‑1 (MQ=255)
                                                                                                           ttgttCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGACGTTTCATGTTTTCCCGATTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGtta                              <  2:3051506/137‑2 (MQ=255)
                                                                                                               tCACCGCCGTTTCTGTCTGTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTGCCCGATTCATACGTTACAGGCGCGTCAAGCAATTTATGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCGGTa                          <  2:2548859/140‑1 (MQ=255)
                                                                                                               tCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTa                          <  2:1678507/140‑1 (MQ=255)
                                                                                                               tCACAGCCGTTTCTGTCTTTTCCATGTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTa                          <  2:2546648/140‑1 (MQ=255)
                                                                                                                        tttCGGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTATGTTCAGTATGTTCAATCGGCTGACGGTTATTTGTATTCTGCTGTTCTGTATGTGAAGAt                 >  2:130620/1‑140 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  1:2787778/140‑1 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  1:2394019/140‑1 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  2:2888437/140‑1 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  1:1418407/140‑1 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  2:790147/140‑1 (MQ=255)
                                                                                                                         ttCTGTCTTTTCCATATCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  2:2286322/140‑1 (MQ=255)
                                                                                                                         gtCTGTCGTTTCCATGTCTAAATCAGAAGCCGGTGCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGAtt                <  1:1597317/139‑1 (MQ=255)
                                                                                                                              tCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGtt           <  1:425441/140‑1 (MQ=255)
                                                                                                                              tCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGATTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGACGATTCTGtt           <  1:1120243/140‑1 (MQ=255)
                                                                                                                                  ttCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGTTTGAt       >  2:2727514/1‑140 (MQ=255)
                                                                                                                                   tCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGTTTGATg      >  1:1885684/1‑140 (MQ=255)
                                                                                                                                       tttCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGTTTGATGTTGa  <  1:243324/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CCTGATTTTGGTCTGATGGCAGATAAGGCGCGATGCCGAGCATCAGACCCCCGCCGATAATCCCGCCTAAAATCGGGCTCAGCCATGCGGCTCTCCGTTTCTTTTCTTTTTTCACAGCCGTTTCTGTCTTTTCCATTTCTAAAGCAGAAGCCGTTTCATGTTTTCCCGCTTCATACGAAACAGGCGCGTCAAGCAATTTCTGTTCAGTATGTTCAATCGGCTGCCGGTTATTTGTATTCTGATGTTCTGTATGTGAAGATTCTGTTTGATGTTGA  >  NZ_CP034943/3193321‑3193595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: