Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,218,214 C→T 5.0% intergenic (‑30/‑174) sigO ← / → EO946_RS20875 RNA polymerase sigma factor SigO/YvrJ family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,218,2140CT 5.0% 378.3 / 7.3 120intergenic (‑30/‑174)sigO/EO946_RS20875RNA polymerase sigma factor SigO/YvrJ family protein
Reads supporting (aligned to +/- strand):  ref base C (45/67);  new base T (3/3);  total (50/70)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 120 total aligned reads displayed.

TCGTCCAATGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCACT  >  NZ_CP034943/3218078‑3218347
                                                                                                                                        |                                                                                                                                     
tCGTCCAATGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTg                                                                                                                                    >  2:1957828/1‑140 (MQ=255)
 cGTCCAATGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGa                                                                                                                                   <  2:1295230/140‑1 (MQ=255)
 cGTCCAATGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGa                                                                                                                                   <  1:2962456/140‑1 (MQ=255)
        tGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCa                                                                                                                            <  1:2488107/140‑1 (MQ=255)
        tGATTTTGCATCTTTTTCGTTCGGGCTTGCCATTCCGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCa                                                                                                                            <  1:465066/140‑1 (MQ=255)
              tGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAAc                                                                                                                      <  1:1804187/140‑1 (MQ=255)
              tGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAAc                                                                                                                      <  1:1875872/140‑1 (MQ=255)
              tGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAAc                                                                                                                      <  1:1824250/140‑1 (MQ=255)
              tGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAAc                                                                                                                      <  1:1815254/140‑1 (MQ=255)
               gCATCTTTTTCGTTAGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACTTTGATAGTCCATCAAAcc                                                                                                                     >  1:2875365/1‑140 (MQ=255)
               gCATCTTTTTCGTTAGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACTTTGATAGTCCATCAAAcc                                                                                                                     >  1:818245/1‑140 (MQ=255)
               gCATCTTTTTCGTTAGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTGACATTTTGACCGTAAAAGACTTTGATAGTCCATCAAAcc                                                                                                                     >  1:305904/1‑140 (MQ=255)
                   cTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCt                                                                                                                 <  2:680908/140‑1 (MQ=255)
                    tttttCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTAACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTc                                                                                                                >  2:2108523/1‑140 (MQ=255)
                        tcgttcgGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCcta                                                                                                            <  1:1456301/140‑1 (MQ=255)
                        tcgttcgGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCcta                                                                                                            <  2:2453665/140‑1 (MQ=255)
                                  tttCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACtaaattaaa                                                                                                  <  2:1944627/140‑1 (MQ=255)
                                     ccATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGa                                                                                               >  2:1671369/1‑140 (MQ=255)
                                     ccATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGa                                                                                               >  2:865729/1‑140 (MQ=255)
                                          aCGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAAGTAAATGAAGGaa                                                                                          >  2:2415587/1‑140 (MQ=255)
                                            gTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAg                                                                                        <  1:1895009/140‑1 (MQ=255)
                                            gTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCGTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAg                                                                                        <  1:41683/140‑1 (MQ=255)
                                              ttttGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCa                                                                                      >  1:1611866/1‑140 (MQ=255)
                                                ttgcaaaaaCGGTAATTCTGAGGATTGCTCAAAAAATGCTTCACGTTCGGATGCTTCATGATCTCCTTACATTTTGAGAGTTATAGACCTTGATAGTCCATCCAGGCGCGTCGCTCCTAAATTGACTGAAGGATAGCaag                                                                                    >  2:2067298/7‑139 (MQ=255)
                                                ttGAACAAACGGTAATGCTGAGGATTGCTCAAAATATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACATTGATAGTCCATCAAACCGCCTCACTACTAAATTAAATGAAGGAAAGCaaa                                                                                    >  2:595213/1‑140 (MQ=255)
                                                ttGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCaaa                                                                                    >  1:211755/1‑140 (MQ=255)
                                                 tGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCacaa                                                                                   >  2:1594455/1‑140 (MQ=255)
                                                 tGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCaaaa                                                                                   >  2:503626/1‑140 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:747536/140‑1 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:1026752/140‑1 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:2865490/140‑1 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:1459785/140‑1 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:2636347/140‑1 (MQ=255)
                                                        aCGGTAATGCTGAGGATTGCACAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTa                                                                            <  1:29661/140‑1 (MQ=255)
                                                             aaTGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAacta                                                                       <  1:1244079/140‑1 (MQ=255)
                                                              aTGCTGAGGATTGCTCAAAAAATGCTTCCCGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAactac                                                                      <  2:1232605/140‑1 (MQ=255)
                                                              aTGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAactac                                                                      <  2:2578125/140‑1 (MQ=255)
                                                              aTGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAactac                                                                      <  2:1064974/140‑1 (MQ=255)
                                                              aTGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGATTCATTATCTCATTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGGCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAactac                                                                      <  2:784456/140‑1 (MQ=255)
                                                               tGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAactact                                                                     <  2:1021131/140‑1 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGt                                                                                                                               >  2:220808/1‑77 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGt                                                                                                                               <  1:220808/77‑1 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACttt                                                                <  1:307356/140‑1 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACttt                                                                <  1:3083717/140‑1 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACttt                                                                <  2:2903653/140‑1 (MQ=255)
                                                                    aGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACttt                                                                >  1:2818381/1‑140 (MQ=255)
                                                                      gATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTCCTTTCa                                                              >  1:1161922/1‑140 (MQ=255)
                                                                      gATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCa                                                              >  2:428549/1‑140 (MQ=255)
                                                                           cTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACAGTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTa                                                         >  2:13533/1‑140 (MQ=255)
                                                                               aaaaaTGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAgcgc                                                     <  2:2141868/140‑1 (MQ=255)
                                                                                aaaaTGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCt                                                    >  2:67445/1‑140 (MQ=255)
                                                                                 aaaTGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCtt                                                   <  1:2897214/140‑1 (MQ=255)
                                                                                    tGCTTCATGATGGTATGCTACATTATCACCTTACATTTTGAACTTAAATGAACTTGATAGTACATAAAACAGGCTCCAGACTAAATTAAATGAAGGAAAGCAAAATAAAGTAAACTACTACTTTCAGACTAGCGCTTGTc                                                <  2:1325376/140‑1 (MQ=255)
                                                                                    tGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCTCCTCCCAACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTc                                                <  2:3055306/140‑1 (MQ=255)
                                                                                    tGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTc                                                <  2:918198/140‑1 (MQ=255)
                                                                                     gCTTCACGAAGTGATGCTTCATTATCTACTTACATTTTTACCCTAAAAGACAATGATAGTCCATAAAACCGCATCCCTACTAAATTAAATGAAGGAAAGCAAATAAAAGTAAAATACTACTTTCTGACTAGAGCTTctct                                               >  2:1653602/1‑140 (MQ=255)
                                                                                        tCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTaa                                            >  1:1058130/1‑140 (MQ=255)
                                                                                            gttGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATAc                                        <  2:1863639/138‑1 (MQ=255)
                                                                                                   tGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGcc                                 >  2:1346554/1‑140 (MQ=255)
                                                                                                    gCTACATTATCTCATTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCGCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCg                                <  2:2349297/140‑1 (MQ=255)
                                                                                                     cTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCGGGTCTTAAATACTCCAGACgg                               >  1:2573092/1‑139 (MQ=255)
                                                                                                           tATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCACAAAAAAGTAAACTACTACTTTCAGACTTGCGCTTGTCTTAAATACTCCAGCCGCCGtttc                         <  2:1247776/140‑2 (MQ=255)
                                                                                                               tcCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAGTAAactact                                                                     <  2:2587729/91‑1 (MQ=255)
                                                                                                               tcCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAGTAAactact                                                                     >  1:2587729/1‑91 (MQ=255)
                                                                                                                ccTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCATCCGCAGTTTACGttt                    >  2:1874370/1‑140 (MQ=255)
                                                                                                                ccTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGttt                    >  1:680617/1‑140 (MQ=255)
                                                                                                                ccTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGttt                    >  2:692311/1‑140 (MQ=255)
                                                                                                                ccTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGttt                    >  2:3005216/1‑140 (MQ=255)
                                                                                                                ccTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGttt                    >  1:1990324/1‑140 (MQ=255)
                                                                                                                  tgaCATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTc                  >  2:1179753/3‑140 (MQ=255)
                                                                                                                   tACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCt                 >  2:862913/1‑140 (MQ=255)
                                                                                                                        tttGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGAt            >  1:1436983/1‑140 (MQ=255)
                                                                                                                        tttGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGAt            >  1:1436240/1‑140 (MQ=255)
                                                                                                                          tGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGAttt          >  2:61162/1‑140 (MQ=255)
                                                                                                                           gACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTc         >  1:2902888/1‑140 (MQ=255)
                                                                                                                            aCCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAgcgc                                                     >  2:2426989/1‑95 (MQ=255)
                                                                                                                            aCCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAgcgc                                                     <  1:2426989/95‑1 (MQ=255)
                                                                                                                            aCCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTcc        <  1:1671369/140‑1 (MQ=255)
                                                                                                                            aCCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTcc        <  1:865729/140‑1 (MQ=255)
                                                                                                                            aCCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTcc        <  1:2685884/140‑1 (MQ=255)
                                                                                                                              cttAAAAGACTTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCCGCCTAGTGTCTTCCAAAATACTTTGCAGCAGTTTACGTTTTATCGGTTTTCCtcac    <  2:305904/138‑4 (MQ=255)
                                                                                                                              cGTAAAAGACTTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCCGCCTAGTGTCTTCCAAAATACTTTGCAGCAGTTTACGTTTTATCGGTTTTCCtcac    <  2:818245/140‑4 (MQ=255)
                                                                                                                              cGTAAAAGACTTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCCGCCTAGTGTCTTCCAAAATACTTTGCAGCAGTTTACGTTTTATCGGTTTTCCtcac    <  2:2875365/140‑4 (MQ=255)
                                                                                                                              cGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCtt      <  1:428549/140‑1 (MQ=255)
                                                                                                                              cGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCtt      <  1:1573714/140‑1 (MQ=255)
                                                                                                                              cGTAAAAGAACTTGAGAGTCCATCAAACCGCCTCCCTACTAAATTAACTGAAGGAACGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCtt      <  1:1088470/140‑1 (MQ=255)
                                                                                                                              cGTAAAACACCTTGCTAGTCCATCAAACCGCCTCCCTACTCAATGAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTGGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCtt      <  1:540382/140‑1 (MQ=255)
                                                                                                                                tAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATttcattca    >  2:1669678/1‑140 (MQ=255)
                                                                                                                                tAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCa    <  1:67445/140‑1 (MQ=255)
                                                                                                                                tAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCa    >  1:385334/1‑140 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  2:2902969/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  2:2902888/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  1:1384747/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  2:2818381/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  1:2687787/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGATTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  1:1528810/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACGAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  1:1707078/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGATAGGCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTTTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  2:180884/140‑1 (MQ=255)
                                                                                                                                 aaaaGACCTTGAGAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCAc   <  2:1647/140‑1 (MQ=255)
                                                                                                                                  aaaGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCACt  <  2:1058130/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
TCGTCCAATGATTTTGCATCTTTTTCGTTCGGGCTTTCCATTACGTTTTTGAACAAACGGTAATGCTGAGGATTGCTCAAAAAATGCTTCACGATGGGATGCTTCATTATCTCCTTACATTTTGACCGTAAAAGACCTTGATAGTCCATCAAACCGCCTCCCTACTAAATTAAATGAAGGAAAGCAAAAAAAAGTAAACTACTACTTTCAGACTAGCGCTTGTCTTAAATACTCCAGCCGCAGTTTACGTTTTCTTAGATTTCCTTCACT  >  NZ_CP034943/3218078‑3218347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: