Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,280,377 G→T 7.2% Q115K (CAA→AAA)  EO946_RS16965 ← lantibiotic dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,280,3770GT 7.2% 466.1 / 3.6 181Q115K (CAA→AAA) EO946_RS16965lantibiotic dehydratase
Reads supporting (aligned to +/- strand):  ref base G (109/52);  new base T (3/16);  total (112/69)
Fisher's exact test for biased strand distribution p-value = 1.67e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

GTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTC  >  NZ_CP034943/3280240‑3280510
                                                                                                                                         |                                                                                                                                     
  aaGCGCGGTCACCATTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                   <  1:2974793/140‑1 (MQ=255)
  aaGCCCGGTCACCCTTACTATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTGTTTGTAAAATGAAGGTTGAttc                                                                                                                                   >  2:2495569/1‑139 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGACAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGAAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                   >  1:1987853/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGTATGTTGATTa                                                                                                                                   >  1:2584015/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTa                                                                                                                                   >  2:265901/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                   >  2:548823/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                   >  1:2995557/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTGTGTAAAATGAATGTTGATTa                                                                                                                                   >  2:3112181/1‑140 (MQ=255)
  aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTGTTGTAAAATGAATGTTGATTa                                                                                                                                   >  1:1211133/1‑140 (MQ=255)
   aGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaa                                                                                                                                  <  2:2762992/140‑1 (MQ=255)
   aGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaa                                                                                                                                  <  2:2608507/140‑1 (MQ=255)
        ggTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAt                                                                                                                             >  2:2569366/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACTATCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  2:1166421/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  1:1025865/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  2:2488361/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  1:364756/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  1:2002480/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                            >  1:1000078/1‑140 (MQ=255)
         gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAAGGAATGTTGATTAAAAGATa                                                                                                                            >  1:2869192/1‑140 (MQ=255)
           cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGAAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                                                          <  2:2150404/140‑1 (MQ=255)
               cTTAATATAACAAGCGGAATTTAATGGAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGAt                                                                                                                      >  2:915193/1‑140 (MQ=255)
                     ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                <  2:2320160/140‑1 (MQ=255)
                       accAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                              <  2:1897888/138‑1 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAATTGATTTTTATCAg                                                                                                             >  2:1956790/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  1:579800/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  2:2274988/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  2:2274986/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  2:3023209/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  2:337138/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  2:2034406/1‑140 (MQ=255)
                        aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                             >  1:170492/1‑140 (MQ=255)
                         cAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGa                                                                                                            >  2:1430354/1‑140 (MQ=255)
                            tcgtaatttaatttAAAAGAAAGTCGGTCGGTTTATTGGTTTTCCATTTTTCTAATTAATTTTTATAGCCAATAAAAATCAACACGCGCATTTTTTTAAATTGATTTTTGATTAAAATATACTTGTTTTTTATCACaaaa                                                                                                         <  2:1009758/136‑1 (MQ=255)
                            gcgtattttATTTTAAAATAAAGTCGTTCGGTTTTTTCGTTTTCAATTTTTATAATTATTTGTTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTTATTAAAAGTTAAGTTATTTTTATAAGaaaa                                                                                                         <  2:429933/134‑1 (MQ=255)
                            gCGTAATTTAATTTAAAATAAAGTCGGTCGGTTTATTCTTTTTCAAATTTTCTAATTAATTGTTAGAGCAACTCAAAATCAACACTAGCCTTTTTTTAAAATGAATTTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                         <  2:1220783/140‑1 (MQ=255)
                            gCGTAATTTAATGTAAAAGAAAGTCGGTCGTTTTTTTCGTTTTCAAATTTTCTAATTATTTGGTATAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAATTTATTTTTATCAGaaaa                                                                                                         <  2:2326647/140‑1 (MQ=255)
                            gCGTAATTTAATGTAAAAGAAAGTCGGTCGTTTTATTCGTTTTCCAATTTTCTATTTATTGGGTAGAGCAAATAAAAATAAACCCGCGCATTTTTGTAAAATCAATGTTTTTTAAAAGATAATTGATTTTTATCAGaaaa                                                                                                         <  2:2248042/140‑1 (MQ=255)
                            gCGGTATTTAATGTAAACGAACGTCGGTCGGTTTTTTCTTTTTCCATTTTTCTATTTATTTGGTTTAGCAACTAAAAATCAACACGAGCCTTTTTGTAAAATTATTGTTGATTAAAAGATATGTGATTTTTATCAGaaaa                                                                                                         <  2:302779/140‑1 (MQ=255)
                            gCGGATTTTAATGTAAAAGAAAGTCGGTCGGTTTATTCTTTTTCCAATTTTCTAATTATTTGGTAGAGCCCCTCAAAATCAACCCGAGCCTTTTTTTAAAATGAATGTTGATTAAACGATAAGTTATTTTTATCAGaaaa                                                                                                         <  2:614184/140‑1 (MQ=255)
                            gCGGAATTTAATTTAAAAGAAAGTCGTTCGTTTTATTCTTTTTCCAATTTTATAATTAAGTGGTAGAGCCAATCAAAATCAAAACGAGCCTTTTTGAAAAATGAATTTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                         <  2:2727004/140‑1 (MQ=255)
                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGacaa                                                                                                         >  1:2815850/1‑140 (MQ=255)
                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                         >  1:2937071/1‑140 (MQ=255)
                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                         >  2:938933/1‑140 (MQ=255)
                            gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCAACTAAAAATCAACACTAGCCTTTTTGTAAAATGAATGTATATAAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                         >  2:652709/1‑140 (MQ=255)
                            gCGGAATTGAATGTAAAAGGAAGTGGTTGGGTGTATGCGTTTTCCATTTTTATATTTATTTTTTTGTGCCACTAAAAATAAACCCGAGCATTTTTTTAAAATCAATGTTGTTTAAAAGATAAGTGATTTTTATCATaaaa                                                                                                         <  2:3084299/140‑1 (MQ=255)
                            ccgtaatttaatttAAACTAACCGCGGTGGGTTTATTCGTTTTCAAATTTTATAATTATTTGTTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGATTGTTGATTAAAATATACGTGATTTTTATCAGaaaa                                                                                                         <  2:2850285/136‑1 (MQ=255)
                             cgtaatttaatttAAAATAAAGTCGTTCGTTGTTTTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCAATCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAc                                                                                                        <  1:364336/137‑1 (MQ=255)
                              gtaaTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTATAATTATTTGGTAGAGCCAATAAAAATCAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAATTGATTTTTATCATAAAACg                                                                                                       <  2:1677147/138‑1 (MQ=255)
                                          aaaaGAAATGGGTTCGGTTTATTCGTTTTCCATTTTTCTATTTAATTGGTAGAGCCACTCAAAAAAAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                                                           <  2:105731/140‑1 (MQ=255)
                                          aaaaGAAAGTCGTTCGTTGTATCGGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACACAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                                                           <  2:1572861/140‑1 (MQ=255)
                                          aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTCAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCgc                                                                                           >  2:1253825/1‑139 (MQ=255)
                                          aaaaCAAAGTCGGTCGTTGTATTCGTTTTCCATTTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACCCGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                                                           <  2:440982/140‑1 (MQ=255)
                                             agaaagTCGGTGGGTTTATTCGTTTTCCTCTTTTATATTTAATGGGTACACCAACAAAAAATCACCCCGACCTTTTTTGAAAAATTATTGTTTATTAAAAGATAAGTGTTTTTTATAACAAAACGACCCTATACCGACTg                                                                                        <  1:789517/140‑1 (MQ=255)
                                                 agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaaga                                                                                    >  1:1888005/1‑140 (MQ=255)
                                                 agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaaga                                                                                    >  1:3104342/1‑140 (MQ=255)
                                                 agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaaga                                                                                    >  1:2516088/1‑140 (MQ=255)
                                                 agTCGGTCGGTGTATTCGTTTTCCAAGTTTCTCATTAATTGGTCGAGCCACTCAAAATCACCACGAGCCTTTGTGTAACATGAATGTTGATTAAAAGATACGTGATGTTTATCAGACAGCGACCCTCTCCCGACTgaaga                                                                                    >  1:2559507/1‑140 (MQ=255)
                                                      gtcggtGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaaaaa                                                                               >  1:234433/1‑140 (MQ=255)
                                                           tttATTCTTTTTCCAATTTTCTAATTATTTGGTAGAGCCAATCAAAAACAACACGAGCCTTTTTGTAAAATTAATGTTGATTAAAACATAAGTGATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCa                                                                          <  1:2132452/138‑1 (MQ=255)
                                                            tttttCTTTTTCAATTTTTCTATTTATTTGTTAGACCCACTCAAACTCAACACGTGACTTTTTGTAAATTGAATGTTTATTAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaa                                                                         <  1:3112181/137‑1 (MQ=255)
                                                             tattgtttttCCAATTTTCTAATTATTTGGTAGACCCACACAAAATCAACCCGCCCCTTTTTGAAAAATGAATGTTTATAAAAAGATACCTGATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                        <  1:1494877/134‑1 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACGCTATACCGACTGAACAGAAAAGGCCACAAGGTGTgg                                                                >  2:1870832/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:1324765/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:1263205/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:21079/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:2228956/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:1777168/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:757801/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:942307/1‑140 (MQ=255)
                                                                     ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTGTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                >  1:1111128/1‑140 (MQ=255)
                                                                       ccAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGtt                                                              >  1:458113/1‑140 (MQ=255)
                                                                            tttCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCttgt                                                         <  2:221086/140‑1 (MQ=255)
                                                                                taattactTGGTAGAGCCAATAAAAATCAACACGAGCCTTTTTGTAAAATCAATGTTTATTAAACGATAAGTTTTTTTTAACAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGct                                                     <  1:1430354/140‑1 (MQ=255)
                                                                                  attaattGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATTTTGATTAAAAGATAAGTGATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                                                   <  2:686473/140‑1 (MQ=255)
                                                                                  attaattGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                                                   <  2:1091951/140‑1 (MQ=255)
                                                                                       ttGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAATGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttg                                              >  1:869303/1‑140 (MQ=255)
                                                                                            cgcgCCACTCAAACTCAACACGACCCTTTTTTTAAAATTCATGTTGATTAAAAGAAAAGTGATTTTTATCCGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                         <  2:2748549/137‑1 (MQ=255)
                                                                                            attccaaCTCAAAATCAACACTAGCCTTTTTGTAAAATCAATTTTGATTAAAAGATAATTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                         <  2:2129676/134‑1 (MQ=255)
                                                                                            agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                         <  1:626319/140‑1 (MQ=255)
                                                                                            agagACACTCAAAATCAACACCCGGCTTTTTTTAAATTGAATTATTATTAAATGATCAGTTTTTTTTAGCAGAAAACGACCCTATACCGACTGAAGAAAACAGGCCAAACGGTGTGGTTATTGTTGCTCTTCTTGTTGCa                                         <  1:915193/140‑1 (MQ=255)
                                                                                            agacCCACTCAAAATCCACACGAGCCTTTTTGTAAAACTATTGGTTATTAAAACACATGTTATTTTTACCAGAACACGACCCTATACCGACTGAAGAAAACAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                         <  1:265901/140‑1 (MQ=255)
                                                                                                 cACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACtt                                    >  1:344243/1‑140 (MQ=255)
                                                                                                 cACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACtt                                    >  1:2876351/1‑140 (MQ=255)
                                                                                                 cACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACtt                                    >  2:2194331/1‑140 (MQ=255)
                                                                                                   cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCTTACTTCa                                  >  1:1082118/1‑140 (MQ=255)
                                                                                                   cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                  >  1:820588/1‑140 (MQ=255)
                                                                                                    tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAACAGATAAGTGATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGGGTGGTTCTTGTTGCTCTTCTTGTTGCATACGTCaa                                 >  2:2384703/1‑140 (MQ=255)
                                                                                                    tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCCGAAAACGACCCTATACCGACTGAAGAAAAAAGTCCAAAAGGTGTGGTTCGTGTTGCTCTTCTTGTTGCATACTTCaa                                 >  2:1296712/1‑140 (MQ=255)
                                                                                                    tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTTTTGCATACTTCaa                                 >  2:2477791/1‑140 (MQ=255)
                                                                                                    tCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaa                                 >  1:1591102/1‑140 (MQ=255)
                                                                                                          tCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                           <  2:1479991/140‑1 (MQ=255)
                                                                                                          acAACACGAGCCTTTTTTTAAAATTAATGTTTATAAAAATATAAGTTATTTTTAACAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                           <  1:228949/139‑1 (MQ=255)
                                                                                                             acacGATGCTTTTTGTAAAATGAATGTTGATTAAAAGATACGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGAGGTTCTTTTTGCTCTTCTTCTTGCATCCTTCAAAATAGCCTg                        >  2:760763/1‑140 (MQ=255)
                                                                                                              cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                       >  2:718492/1‑140 (MQ=255)
                                                                                                              cacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTAGACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGt                       >  1:1569283/1‑140 (MQ=255)
                                                                                                                cGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTg                     >  1:2798798/1‑140 (MQ=255)
                                                                                                                            tAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtt         >  2:17644/1‑140 (MQ=255)
                                                                                                                            tAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtt         >  2:3005137/1‑140 (MQ=255)
                                                                                                                                  gaattTTGATTAAAATATACGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttttttt   <  1:1478085/140‑1 (MQ=255)
                                                                                                                                   aatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTc  >  2:86882/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTC  >  NZ_CP034943/3280240‑3280510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: