BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,647	C→A	34.4%	V252L (GTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,647	0	C	A	34.4%	626.2 / inf	507	V252L (GTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base C (167/165); new base A (122/53); total (289/218)
Fisher's exact test for biased strand distribution p-value = 3.09e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 519 total aligned reads displayed.

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285508‑3285784

     tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTa                                                                                                                                      <  2:2626339/140‑1 (MQ=255)

     tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGATTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                      <  1:2884562/140‑1 (MQ=255)

         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCACTCACTTCTCCATTGTATACATCTAatat                                                                                                                                  >  2:1986825/1‑140 (MQ=255)

         taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAatat                                                                                                                                  >  2:626735/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                             >  1:1127547/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTgg                                                                                                                             <  2:151717/140‑1 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                                                                                                      <  2:2775892/131‑1 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                             >  2:1117449/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                             >  1:612204/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTCTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                                                                                                             >  2:2470800/1‑140 (MQ=255)

              aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTGGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTAAAAATCTAATATACTgg                                                                                                                             >  1:908819/1‑140 (MQ=11)

               gAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGa                                                                                                                            >  1:1160847/1‑140 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  2:159636/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  1:2574513/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  2:1092837/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  1:2029264/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  1:1903784/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  1:1563636/140‑1 (MQ=255)

                aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                                                                                                           <  1:1221136/140‑1 (MQ=255)

                   cgtGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCAGTGTATACATCTAATATACTGGAAAgg                                                                                                                        >  2:1282894/3‑140 (MQ=255)

                   cTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACCGGAAAgg                                                                                                                        <  2:926415/140‑1 (MQ=255)

                     tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAATGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                                                                                                           >  2:1717973/1‑135 (MQ=11)

                      ggTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAc                                                                                                                     >  1:1835896/1‑140 (MQ=255)

                        tCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCGGCTGAAGTGTGTCTAAGACAAAGTCTGTGTCCAGCTTATCCCCAATCGTAACAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACaa                                                                                                                   <  2:2183797/140‑1 (MQ=11)

                         cACTATGTAACACGCAGTTCTCAGGGAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGATTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAAt                                                                                                                  <  2:1665548/140‑1 (MQ=255)

                          aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAtt                                                                                                                 >  1:2216865/1‑140 (MQ=255)

                            tATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGt                                                                                                               >  2:1177177/1‑140 (MQ=255)

                                tAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCt                                                                                                           <  1:2745786/140‑1 (MQ=255)

                                      gcggTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                     >  2:876126/4‑140 (MQ=11)

                                      gCAGTTCTCAGGCCGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                     >  2:250985/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                     >  1:540712/1‑140 (MQ=255)

                                      gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                                                                                                     >  1:2797048/1‑140 (MQ=255)

                                       cAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATa                                                                                                    <  2:2098397/140‑1 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAATGTAAAATTGTTTCTGTACATaa                                                                                                   >  2:2348676/1‑140 (MQ=11)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCt                                                                                                           <  1:633849/132‑1 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                   >  1:1964469/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                                                                                                   >  2:2903191/1‑140 (MQ=255)

                                        aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAGaa                                                                                                   >  1:2338683/1‑140 (MQ=255)

                                          ttCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                 <  1:843263/140‑1 (MQ=255)

                                          ttCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAgg                                                                                                 <  1:1049592/140‑1 (MQ=255)

                                              cAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTACTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa                                                                                             <  1:1670416/140‑1 (MQ=11)

                                              cAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa                                                                                             <  1:139560/140‑1 (MQ=255)

                                              cAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa                                                                                             <  1:953340/140‑1 (MQ=255)

                                              cAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa                                                                                             <  1:2940536/140‑1 (MQ=255)

                                               aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAat                                                                                            <  2:1885903/140‑1 (MQ=255)

                                                ggCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAata                                                                                           >  1:1492502/1‑140 (MQ=255)

                                                ggCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAata                                                                                           >  2:2255679/1‑140 (MQ=255)

                                                    gtgtTGGTAGCTGATGAAGTGTGTCTATGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAATAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGGCCATAAGGCAAGTAtgtg                                                                                       >  1:2831450/1‑140 (MQ=11)

                                                    gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                       >  1:2627621/1‑140 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                       <  2:420343/140‑1 (MQ=255)

                                                    gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg                                                                                       >  2:1053754/1‑140 (MQ=255)

                                                     tgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGa                                                                                      <  1:1249692/140‑1 (MQ=255)

                                                        tGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGt                                                                                   <  2:1940109/140‑1 (MQ=255)

                                                               tcaTTAAGTATGTCTAAGACACTCTCTCTGTCCAGCTTATCCCCAATCCTAAAAGCAATCACGTCTCCATTGTATACATCAAATAAACTGCAAAGGTACCATTCTATCTGTCCATAACGAAAAAATCTGAAGTCCGTTAc                                                                            >  2:2525446/3‑140 (MQ=255)

                                                               tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                            >  1:2496683/1‑140 (MQ=255)

                                                               tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                            >  1:682623/1‑140 (MQ=255)

                                                               tGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTAc                                                                            >  1:2249014/1‑140 (MQ=255)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa                                                                          >  1:1011173/4‑140 (MQ=12)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa                                                                          >  2:2714503/4‑140 (MQ=12)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa                                                                          >  1:2316562/4‑140 (MQ=12)

                                                                 atgaaGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTa                                                                          >  2:1427057/4‑140 (MQ=255)

                                                                 aTTAAGTATGTCTAAGACAAAGTCTGTGTCCGGCTTATCCCCACTCGTAAAAGCAATCACTTCTCCAGTGTATACATCTAATATACAGGAACGGTACAATTGTGTCTGTCCATAAGGCCACTATGTGAGGTCCGTAACtc                                                                          >  2:2392319/1‑139 (MQ=14)

                                                                  tgaaGTGTGTCTAAGACAAAGTCTGTGTCCTTCTTATCCCCAATCGTAAATGCGATCACTTCTCCATTGTATAAATCTAATATACTGGAACGGTACAATTGTTTATGTCCATAAGGAAAATATGAGAGGTCGGttccgac                                                                         >  2:2636412/3‑135 (MQ=11)

                                                                   gaaGTGTGTCTAAGACAAGGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTaat                                                                        >  2:1333813/2‑138 (MQ=255)

                                                                         tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                  >  1:212461/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                      >  2:523017/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                      >  1:1752413/1‑140 (MQ=40)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAGGTGATGTCCGTTACTAGTTGTTCAAGAGGGCGATCa                                                      >  1:3024497/1‑140 (MQ=255)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                      >  2:1149155/1‑140 (MQ=21)

                                                                                     aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGAAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                      >  2:2055995/1‑140 (MQ=255)

                                                                                      gTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAg                                                     <  1:2364290/140‑1 (MQ=40)

                                                                                      gTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAg                                                     <  1:1546175/140‑1 (MQ=40)

                                                                                           tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCACTAGGGCGATCAGACTGa                                                >  2:1573090/1‑140 (MQ=255)

                                                                                           tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGa                                                >  2:1376313/1‑140 (MQ=21)

                                                                                                 gCTTATCCCCAATCGTAAACGCAATCACTTCTCCATTGTATAAATCTAATAGACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                          <  1:24399/140‑1 (MQ=255)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                          <  1:326819/140‑1 (MQ=40)

                                                                                                 gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGGTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTc                                          >  1:3089414/1‑140 (MQ=255)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGATAGGTACAATTGTTTCTGTCCATCAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                        <  1:1284787/140‑1 (MQ=255)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                        <  1:1694897/140‑1 (MQ=40)

                                                                                                   ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCg                                        <  1:486893/140‑1 (MQ=40)

                                                                                                     aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGAt                                      >  1:361507/1‑140 (MQ=38)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                     <  1:394310/140‑1 (MQ=37)

                                                                                                      tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                     <  2:337239/140‑1 (MQ=37)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                    >  1:2686999/1‑140 (MQ=37)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATcc                                    >  1:584579/1‑140 (MQ=14)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                     <  2:716930/139‑1 (MQ=14)

                                                                                                       ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATc                                     >  1:716930/1‑139 (MQ=14)

                                                                                                        cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCa                                   <  2:2813243/140‑1 (MQ=35)

                                                                                                          cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                 <  2:384678/140‑1 (MQ=34)

                                                                                                          cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                 <  2:1793243/140‑1 (MQ=34)

                                                                                                          cAATCGTAAAAGCAATCACTTCTCCATTGGATACATCTAATATACTGGAAAGGTACAAGTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                 <  2:577885/140‑1 (MQ=21)

                                                                                                          cAATCGTAAAAGCAATCACTTCTACATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAat                                 <  2:755466/140‑1 (MQ=21)

                                                                                                                tAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAtatta                           >  2:679289/1‑140 (MQ=32)

                                                                                                                  aaaGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATc                         <  1:2532358/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCTGTGTATACATCTAATAGACTGGAAATGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                    <  2:2559415/140‑1 (MQ=255)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                    <  1:1080318/140‑1 (MQ=32)

                                                                                                                       aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCa                    <  1:2178640/140‑1 (MQ=32)

                                                                                                                       aaTCACGTCTCCATTGTATACATCTCATATACTGGAAAGCTACAATTGTTTCGGTCCATAAGGCAAATCTGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATAGATTATCGACCa                    <  2:3041003/140‑1 (MQ=11)

                                                                                                                          cACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACgg                 <  1:780801/140‑1 (MQ=32)

                                                                                                                                  cATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGc         <  2:147620/140‑1 (MQ=32)

TAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285508‑3285784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`