Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,394,360 T→G 10.9% K180N (AAA→AAC ppaX ← pyrophosphatase PpaX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,394,3600TG10.9% 559.0 / 13.6 236K180N (AAA→AACppaXpyrophosphatase PpaX
Reads supporting (aligned to +/- strand):  ref base T (78/123);  new base G (33/2);  total (111/125)
Fisher's exact test for biased strand distribution p-value = 2.04e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

CGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAA  >  NZ_CP034943/3394222‑3394499
                                                                                                                                          |                                                                                                                                           
cGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCCAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGttt                                                                                                                                            <  2:708102/140‑1 (MQ=255)
cGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGttt                                                                                                                                            <  2:3052346/140‑1 (MQ=255)
  gATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGAGTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGtgttt                                                                                                                                          >  1:1038185/1‑140 (MQ=255)
      aCGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTc                                                                                                                                      <  2:1139413/140‑1 (MQ=255)
                 ttCTCACAGATTACTTCACTCCAACTATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  2:2695825/140‑1 (MQ=255)
                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  2:2130558/140‑1 (MQ=255)
                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCATTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  1:988274/140‑1 (MQ=255)
                 ttCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAACCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTc                                                                                                                           <  1:2015715/140‑1 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCa                                                                                                                    >  2:2223822/1‑140 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCa                                                                                                                    <  1:1617591/140‑1 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCa                                                                                                                    >  2:2988889/1‑140 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCa                                                                                                                    >  2:526/1‑140 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTtgcctgccc                                                                                                                    >  2:2345204/1‑139 (MQ=255)
                        aGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTACGGCGTTTTTACCTGCCa                                                                                                                    >  2:1234454/1‑140 (MQ=255)
                            tACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTCCCTGCCAGcac                                                                                                                >  2:1542088/1‑140 (MQ=255)
                            tACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTGCCTTCCAGcac                                                                                                                >  2:2029347/1‑140 (MQ=255)
                            tACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTCCGGCGTTTTTGCAAGGCAGcac                                                                                                                >  2:1923553/1‑140 (MQ=255)
                              cTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACAt                                                                                                              <  2:258971/140‑1 (MQ=255)
                              cTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACAt                                                                                                              <  2:2621261/140‑1 (MQ=255)
                                  aCTCCATCGATTGGTAATAGATCACTCATTTTCTCAAGCATATACTCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  2:2051723/140‑1 (MQ=255)
                                  aCTCCAACGATTTGTAATAGATCACTCCTGTTCTCAAGCATATAATCAGGCTCCTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  1:2123963/140‑1 (MQ=255)
                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  2:2258888/140‑1 (MQ=255)
                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  1:2308354/140‑1 (MQ=255)
                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  2:1824297/140‑1 (MQ=255)
                                  aCTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTg                                                                                                          <  1:2883565/140‑1 (MQ=255)
                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTAACTGCCAGCACATCGTGGt                                                                                                        >  1:2304979/1‑140 (MQ=255)
                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTTCCGGCCAGCACAGCGTggg                                                                                                        >  1:130291/1‑139 (MQ=255)
                                    tCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGGTTTTGACTGCCCGCACATCGTGGt                                                                                                        >  1:1351162/1‑140 (MQ=255)
                                      cAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTACCAGCCCGCACAGCGTGGTaa                                                                                                      >  2:189940/1‑140 (MQ=255)
                                        aCGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTCCGGCGTTTTTCCCTGCCCGCACATCGTGGTAAtt                                                                                                    >  1:916464/1‑140 (MQ=255)
                                        aCGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTGTAATCGTCCATGCGACTCCGGCCGTGGTTTGTACGGGGGTGTTCCCGGGCCGCACCTGGTGGTAAtt                                                                                                    >  1:502770/1‑140 (MQ=255)
                                            tttGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTAtct                                                                                                >  2:3008804/1‑140 (MQ=255)
                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCTGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  1:311042/140‑1 (MQ=255)
                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  2:1745875/140‑1 (MQ=255)
                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  1:2223822/140‑1 (MQ=255)
                                              tGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTcc                                                                                              <  1:1193244/140‑1 (MQ=255)
                                                     gATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGGGTTTTTCCCTGGCAGCACATGGTGGTAATTTTCTGCGAACATa                                                                                       >  2:1599856/1‑140 (MQ=255)
                                                      aTCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGGGTTTTTACCTGACAGCAAAACGTGGTAATTATATACGACCATaa                                                                                      >  1:727034/1‑140 (MQ=255)
                                                      aTCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTGCCTGCCACCACAACGTGGTAATTATATCAGACCATaa                                                                                      >  1:621158/1‑140 (MQ=255)
                                                        cACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAAtt                                                                                    >  2:2877104/1‑139 (MQ=255)
                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGGGGTTTTGCCGGGCCGCACATGGTGGTAATTATGTCCGACCAAAAttgg                                                                                  >  1:1429877/1‑137 (MQ=255)
                                                          cTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGGGTTTTTCCCTGCCCGCACATCGTGGTAATTATCTCCGACCATAATggg                                                                                  >  2:1154806/1‑139 (MQ=255)
                                                           tCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGGGGTTTTGCCTGCCAGCACCACGGGGTAAATATCTCCGACCATAAtaggt                                                                                 >  1:24033/1‑136 (MQ=255)
                                                           tCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGGTTTTGCCAGCCAGCACATCGGGGGAAATAAATCCGACCATAATgggt                                                                                 >  1:1552476/1‑138 (MQ=255)
                                                             aTTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGTTTTTACCAGCCAGCACATAGTGGTTATTATCTACGACCAAAATTGCtta                                                                               >  2:1869187/1‑139 (MQ=255)
                                                             aTTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATATACGACCATaaatggttc                                                                               >  2:2007039/1‑133 (MQ=255)
                                                                    cAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTGCCTGCCCGCACCTCGGGGTAATTATATCCGACCATAATGGCTTGCGCCGGc                                                                        >  1:1412286/1‑140 (MQ=255)
                                                                     aaGCCTATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                       <  1:3056426/140‑1 (MQ=255)
                                                                     aaGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCt                                                                       <  2:2959246/140‑1 (MQ=255)
                                                                       gCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCg                                                                     <  1:1966616/140‑1 (MQ=255)
                                                                             aaTCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGt                                                                                                                                >  1:524678/1‑75 (MQ=255)
                                                                             aaTCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTGGCGGCGGTTTTCCCTGCCAGCACATCGGGGTAATTATCTCCGGCCATAATGGCTTACGCCGGCTCGcgtgcg                                                               >  2:2454374/1‑135 (MQ=255)
                                                                             aaTCAGGCTCATGCTTTGCCAGGGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTCCGGCGTGTTTCCCTGCCAGCCCATCGTGGTAATTATCCCCGACCATCATGGCTTCCGCCGgcgcgcgtgct                                                               >  2:1213808/1‑135 (MQ=255)
                                                                                cAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTACATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGc                                                            <  2:590018/140‑1 (MQ=255)
                                                                                 aGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTTCGGCGTTTTTGCCTGCCAGCCCCTCGGGGTAATTATCTCCGACCATAATTGCTTCCGCCGGCTCGCTTCCTAGcg                                                           >  2:893818/1‑139 (MQ=255)
                                                                                 aGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTACCTGCCAGCCCATCGTGGGAATTATCAACCACCATAATGGCTTCCGCCGGCTCGCGTACTAGCt                                                           >  2:1458951/1‑140 (MQ=255)
                                                                                  ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTACCTGCCAGCACATCGGGGTAATTATATCCGACCCTAATGGCTTACGCCGGCTCGCTTCCTAGcgg                                                          >  2:2353900/1‑138 (MQ=255)
                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTGCCTGCCAGCACAACGTGGTAATTATCTCCGACCATAATGGCGTCCGCCGGCTCGCTTCCTAGCTGt                                                         >  2:463615/1‑140 (MQ=255)
                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTACCTGCCCGCACAGCGTGGTAATTATATCCGCCCATAATGGCTTACGCCCGCTCGCGTCCTAGCTGt                                                         >  2:501506/1‑140 (MQ=255)
                                                                                   gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTACCTGCCAGCACATCGTGGTAATTATCTACGACCATAATTGCTTCCGCCGGCGAGCTTCCTAGCTGt                                                         >  2:1330470/1‑140 (MQ=255)
                                                                                       aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCa                                                     <  1:1234143/140‑1 (MQ=255)
                                                                                       aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTTCGGCGGTTTTGCCGGCCCGCCCCACGTGGTAATTATCTCCGACCATAATTGGGTGCGCCGGGGCGCGTGCTATCTGgttag                                                     >  1:1924016/1‑138 (MQ=255)
                                                                                        tGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAACACATCGTGGTAATTATATACCACCAAAATGGCTTGCGCCCGCTCCCATGCTAGCTGGGTCAg                                                    >  2:2545752/1‑140 (MQ=255)
                                                                                        tGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTACCTGCCAGGACATCGTGGTAATTATCTCCCACCATAAAGGCTTCCGCCGGCTCGCGTCCTAGCGGTTTAAg                                                    >  2:908543/1‑140 (MQ=255)
                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTGCGGCGGTTTTGCCGGCCCGCACAAAGTGGTAATTATATGCGACCAAAAATGCTTACGCCCGCTCGCTTACTAGCGGTTTAAGAGCTa                                               >  1:2187630/1‑140 (MQ=255)
                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGGGTTTTTCCCAGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTa                                               >  2:1543771/1‑140 (MQ=255)
                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTTCGGGGGGTTTGCCTGGCAGCCCAACGGGGTAATTATATCCGACCCAAAAGGGGTGCGGCCGCGCGGTGGCGAGCTGTTTCAgcgcga                                               >  1:2625131/1‑138 (MQ=255)
                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGGGTTTTTTCCTGCCACCACATCGGGGGAATTATCTCCGACCATAAAGGGGTGCGCCCGCGCGCTTCCTAGCTGTTTGAGCGCTa                                               >  1:2782365/1‑140 (MQ=255)
                                                                                             tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTACGGCGTTTTTCCCAGCCAGCACATCGTGGTAAATATCTCCGACCATAAAGGCTTCCGCCGGCTCGCTTCATAGCTGTTTCAGCGCTa                                               >  2:1722540/1‑140 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTGCCTTCCAGCACATAGTTGTAAATATAGCCGCCCATAATGGCTTCCGCCGGCGCGCTTACAAGATGTTTAATCCCTAAAAGcc                                         >  2:410923/1‑139 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGGGTTTTTACCGGCCCGCCCATCGTGGTAATTATCTCCGACCATAATGGCTTACGCCGGCTCGCTTGCAAGCTGTTTAAGCGCTAAAAAca                                         >  2:962892/1‑140 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTGTCCGGCGTTTTTCCCTGCCACCACATCGTGGTAATTATCTACGACCATAATGGCTTCAGCCGGCTCGCTTCCTAGCTGGTTCAGCGCTAAAAGca                                         >  2:872824/1‑140 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                         <  1:2386850/140‑1 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                         <  2:2979399/140‑1 (MQ=255)
                                                                                                   tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTTCCGGCCACCACAACCTGGTAATTATATGCGACCAAAAATGGGTCCGCCCGCGCGCGTGCGAGCGGTTTAAGCGCGAAAAAca                                         >  1:424110/1‑140 (MQ=255)
                                                                                                    gtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCCGCACATCGTGGTAATTATCTCCGACCATAATGTCTTACGCCGGCTCGCTTCCAAGCTGTTTCAGCGCTAAAAGcac                                        >  2:1402203/1‑140 (MQ=255)
                                                                                                    gtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTACCTGCCAGCACATCGTGGTAATTATATCCGACCATAATTGGTTCCGCCGGCTCGCTTCCTAGCAGTTTCAGCGCTAAAAGcac                                        >  2:2764273/1‑140 (MQ=255)
                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGTCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATCATGGCTTCCGCCGGCTCGCTTCCTAGCTGTGTCAGCGCTAAAAGCACAGGCt                                   <  1:2762498/140‑1 (MQ=255)
                                                                                                         cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                   <  2:2818318/140‑1 (MQ=255)
                                                                                                               tttACTCGTCCATGCGACTCCGGCCGTTTTTGGTCCGGCGTTGTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  2:2153589/140‑1 (MQ=255)
                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATTGCTTCCGCCGGCGCGCGTCCTAGCGGTTTCAGCGCGAAAAGCACAGGCTCGGGAt                             >  2:3062191/1‑140 (MQ=255)
                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  1:1869187/140‑1 (MQ=255)
                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  1:2277279/140‑1 (MQ=255)
                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  1:1145128/140‑1 (MQ=255)
                                                                                                               tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                             <  2:2304979/140‑1 (MQ=255)
                                                                                                                     cGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCt                       <  1:1718250/140‑1 (MQ=255)
                                                                                                                         cATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTGCCTGCCAGCCCAGCGTGGTAATTATCTCCGACCATAATTGCTTCCGCCGGCTCGCTTCCTAGCTGTTTGAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                   >  2:2532260/1‑140 (MQ=255)
                                                                                                                         cATGCGACTCCGGCCGTTTTTGTTCCGGGGTTTTTCCCAGCCCGCACATCGTGGTAATTATATACCACCATAAATGCTTCCCCCGGCTCGCTTACTAGCTGTTTCAGCGCGAAAACCACAAGCTAGGGATCAGGCTTTGc                   >  2:2078939/1‑140 (MQ=255)
                                                                                                                         cATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                   <  1:1822154/140‑1 (MQ=255)
                                                                                                                         cATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTACCTGCCCGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTGCTACCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                   >  2:2268701/1‑140 (MQ=255)
                                                                                                                                cTCCGGCCGTGTTTGTTCCGGCGTTTATCCCGGCCAGCACCTGGTGGTAATTATCTGCGAACATAATGGCGTCCGCCGGCTCGCTTCCTCGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTc            <  2:1800345/140‑1 (MQ=255)
                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTTGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTGAGCGATAAAAGCACAGGCTCGGGATCAGGATTTTCCAtgtttag          <  1:2345204/140‑7 (MQ=255)
                                                                                                                                  ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAACCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  2:342146/140‑1 (MQ=255)
                                                                                                                                  ccGGCCGTTTTTGGTCCGGCGTTTTTCCCTGCCAGCACATCGTGTTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  1:2677568/140‑1 (MQ=255)
                                                                                                                                  ccGGCCCTGTTTGTGCCGGCGTGTTTCCCTGCCAGCACATCGTGGTAATTCTCTCCGACCATCATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTCCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac          <  2:1770259/140‑1 (MQ=255)
                                                                                                                                   cGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca         <  2:1697565/140‑1 (MQ=255)
                                                                                                                                   cGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTACTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca         <  2:637191/140‑1 (MQ=255)
                                                                                                                                     gCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAAATATCTCCGACCATAAAGGCTTCCCCCCGCTCGCTTCCAAGCTGTTTCAGCGCTAAAACCACAGGCTCGGGAACAGGCTTTGCCATCGTcacagc       >  2:2200223/1‑138 (MQ=255)
                                                                                                                                         tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca   <  1:66944/140‑1 (MQ=255)
                                                                                                                                          ttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGGTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGCCTCGGGATCAGGCTTTGCCATCGTCACATCATCaa  <  1:1032328/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CGGATGACGATCTGTTTTTCTCACAGATTACTTCACTCCAACGATTTGTAATAGATCACTCATTTTCTCAAGCATATAATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAA  >  NZ_CP034943/3394222‑3394499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: