Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,277 C→G 5.3% A40A (GCC→GCG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,2770CG 5.3% 468.5 / 3.6 167A40A (GCC→GCGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base C (73/80);  new base G (10/2);  total (85/82)
Fisher's exact test for biased strand distribution p-value = 3.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

CCACAAGGACCACAAGGACTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACC  >  NZ_CP034943/607140‑607415
                                                                                                                                         |                                                                                                                                          
ccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGAc                                                                                                                                          <  2:1199479/140‑1 (MQ=255)
ccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                                                                                          <  2:686059/140‑1 (MQ=255)
ccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                                                                                          <  2:2499531/140‑1 (MQ=255)
ccacaaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                                                                                          >  1:1177487/1‑140 (MQ=255)
   caaggaccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACggg                                                                                                                                       <  1:47881/140‑1 (MQ=255)
        accagcaggacCGCCGGGAGTCAGGGGAGCGACCGGCGCTGCCGGTGAAGCCGGACCACCCGGCGCGACCGGAGCCACGGGAGCTACTGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                                  <  2:1934536/134‑1 (MQ=11)
        accacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCGACGGGAGCTCCGGGGGCCGCCGGAGCTGCCGGAGCCGCCGGGGCTGCGGGAGCACCGCGAGcgc                                                                                                                                  >  1:1116096/1‑138 (MQ=18)
         ccacaaggacTGCCGGGAGTCATGTGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACCGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGCAGCGACGGGAGCCACGGGAGCTAc                                                                                                                                 >  1:1902071/1‑140 (MQ=255)
         ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTAc                                                                                                                                 >  1:3074999/1‑140 (MQ=255)
         ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTAc                                                                                                                                 >  1:1406422/1‑140 (MQ=255)
         ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACCGGAGCCACCGGAGCAAc                                                                                                                                 >  2:1468268/1‑140 (MQ=255)
         ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCCGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTAc                                                                                                                                 >  1:67864/1‑140 (MQ=255)
         ccacaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTAc                                                                                                                                 >  1:2960822/1‑140 (MQ=255)
           acaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCGACGGGAGCTAGTGGGGAAGCGGGACCAGCCGGACCAGCGGGAGCGACGGGCGCCCAGGGAGCTACCg                                                                                                                               >  2:577376/1‑140 (MQ=25)
           acaaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCg                                                                                                                               >  2:829169/1‑140 (MQ=255)
            cacggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCGACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                                              <  2:1262939/137‑1 (MQ=255)
            caaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                                              <  2:1014924/140‑1 (MQ=255)
             caggacGGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGACGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                             <  1:1157169/139‑1 (MQ=255)
             aaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                             <  1:1084339/140‑1 (MQ=255)
             aaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                             <  2:2804236/140‑1 (MQ=255)
             aaggacTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCggg                                                                                                                             <  1:2365521/140‑1 (MQ=255)
                 acTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAg                                                                                                                         <  1:255264/140‑1 (MQ=255)
                    gCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCATCCGGGCCGACAGGAGCCACGGGAGCTACTGGGGAAGCGGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGcgg                                                                                                                      >  1:544218/1‑138 (MQ=255)
                    gCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCTACAGGAGCCACGGGAGCGACTGGCGACTCTGGCCCAGCCGGACCTGCGGGCGCGACGGGAGCGCCCGGAGCTACCGGGGAAGcag                                                                                                                      >  1:863728/1‑138 (MQ=18)
                    gCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTg                                                                                                                      >  1:2168062/1‑140 (MQ=255)
                    gCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACACGAGCCACGGCAGCGCAGCGGGCAGCGGCACCAGCCGGACCGCCAGGCGCGACCGGAGCGGCGGGAGCGCGCGGGGAAGCTg                                                                                                                      >  1:171137/1‑140 (MQ=16)
                      cGGGAGTCATGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCGACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGa                                                                                                                    <  2:217369/140‑1 (MQ=255)
                       gggAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGAc                                                                                                                   <  2:1464354/140‑1 (MQ=255)
                           gTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGc                                                                                                               <  2:471260/140‑1 (MQ=255)
                            tCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGcc                                                                                                              <  1:2238851/140‑1 (MQ=255)
                            tCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAACCACGCGAGCTCCTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGcc                                                                                                              <  1:759067/140‑1 (MQ=255)
                              aTGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCAAGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCgg                                                                                                            <  2:2932260/140‑1 (MQ=255)
                               tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGa                                                                                                           >  1:1778877/1‑140 (MQ=255)
                               tGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGCAAGCTGGACCAGCCGGa                                                                                                           >  1:2517407/1‑140 (MQ=255)
                                   aGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTCCCGGGGAAGCAGGACCAGCCGGAGCGa                                                                                                       >  2:1542612/1‑140 (MQ=255)
                                    gccatctgagaTACCGGTTAATCCGGACCAGACTGTGCGACTGGAGCCACGGGAGCTACTGGTGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACTGGAGCTACCGGGGAAGCTGGACCAGCCGTAGCGAc                                                                                                      <  2:178840/133‑1 (MQ=17)
                                    gCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGAc                                                                                                      <  2:651540/140‑1 (MQ=255)
                                     ccACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACCGGAGCCACGGGAGCTACTGGGGAAGCTGGTCCAGCCGGAGCAGCCCGAGCCACGGGCGCCACGGGACCTACCGGGGAAGCTGGAGCAGCCGGAGCGACg                                                                                                     >  2:2624672/1‑140 (MQ=21)
                                      cacaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACgg                                                                                                    >  2:1175767/1‑140 (MQ=255)
                                      cacaGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACgg                                                                                                    >  2:2977448/1‑140 (MQ=255)
                                        caGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACgggc                                                                                                  >  1:643995/1‑139 (MQ=255)
                                        caGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGa                                                                                                  >  1:564123/1‑140 (MQ=255)
                                             gCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGAc                                                                                             <  2:374253/140‑1 (MQ=255)
                                              cTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGCGCTACCGGGGACGCTGGACCAGCAGGAGCGTCGGGCGCGACa                                                                                            >  1:1838501/1‑140 (MQ=18)
                                                aCCGGGGAAGCCGGACCCGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                                          <  2:2730505/140‑1 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCGGGACAAGCCGGAGCGACGGGAGCGACAgg                                                                                          >  1:444293/1‑140 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                                          <  2:1020258/140‑1 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAgg                                                                                          <  1:2587095/140‑1 (MQ=255)
                                                aCCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACGGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACCGGAGATACCGGGGACGCTGGACCCGCCGGAGCGACGGGAGCGAGAgg                                                                                          >  1:513122/1‑140 (MQ=18)
                                                 ccGGGGCACCCGGACCACCCGGGGCCCCAGGAGCCACCGGAGCTACTGGGGAACCCGGACCAGCCGGACCAGCCGGAGCCACGGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                                         <  2:248665/140‑1 (MQ=14)
                                                     ggAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTa                                                                                     <  2:1683051/140‑1 (MQ=255)
                                                     ggAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTa                                                                                     <  1:829169/140‑1 (MQ=255)
                                                     ggAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTa                                                                                     <  1:2876916/140‑1 (MQ=255)
                                                        aGCCGGACCAGCCGGGGCGACAGGAGCCACCGGAGCTCCGGGGGAAGCGGGACCAGCCGGACCAGCCGGCGCGACGGGCGCGACCGGAGCAACCGGGGCAGCTGGCCCAGCCGGCGCGAGGGGGGGGGCCGGAGCTGCCg                                                                                  >  2:2825205/1‑140 (MQ=19)
                                                         gCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGTGCAGCCGGACCACACTGAGCGAACTCCGCCACGGGAGCTAGCGTGGAAGCCGGAGAAGCCGGAGCGACGGGAGCGACAGGGGCTCCggg                                                                                >  1:2159674/1‑140 (MQ=18)
                                                         gCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACg                                                                                                     >  2:2618677/1‑120 (MQ=255)
                                                         gCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACg                                                                                                     <  1:2618677/120‑1 (MQ=255)
                                                                       ggCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccag                                                                   <  1:1169207/140‑1 (MQ=255)
                                                                       ggCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccag                                                                   <  1:260648/140‑1 (MQ=255)
                                                                           aCAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGAGGGGAGCGACAGGAGCTACCGGGGAtgccggaccagccgg                                                               <  1:2131508/140‑1 (MQ=255)
                                                                           aCAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgg                                                               <  1:2233791/140‑1 (MQ=255)
                                                                           aCAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgg                                                               <  1:846142/140‑1 (MQ=255)
                                                                            cAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgga                                                              <  1:1538953/140‑1 (MQ=255)
                                                                            cAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgga                                                              >  2:205174/1‑140 (MQ=255)
                                                                            cAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgga                                                              <  1:1013779/140‑1 (MQ=255)
                                                                            cAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAagccggaccagccgga                                                              <  1:1010133/140‑1 (MQ=255)
                                                                                 gCCTCGGGAGCTAATGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGATACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                         <  2:1902071/140‑1 (MQ=255)
                                                                                 gCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAc                                                         >  1:2327373/1‑140 (MQ=255)
                                                                                  ccACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGACCGACCGGCGCGACCGGAGCTACCGGGGACGCCGGACCACCCGgaccgacc                                                        >  1:971021/1‑140 (MQ=18)
                                                                                    aCGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACCGGAGCAACCGCGGCAGCACGACCATCCGGAGCGACCGGAGCGACAGGAGCTACCGGTCACTCCGCCCCGGCCCGAGCAACCgg                                                      >  2:1615874/1‑140 (MQ=14)
                                                                                    aCGGGAGCTACTGGGGAAGCAGGACCATCCGGACCATCCGGAGCGACGGGAGCCACCGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACAGGTGAAGCCGGACCATCAGGACAAACCga                                                      >  1:1618457/1‑139 (MQ=17)
                                                                                     ggggAGCGACTGGTGACTGGGGACCAGGCGGCCCAGCGGTAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGa                                                     <  2:458730/139‑1 (MQ=17)
                                                                                     cGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGAGACACGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACTGGa                                                     >  2:737313/1‑140 (MQ=17)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACTGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACAGGGGAAGCCGGACCAGCCGGAGCAACGGGAGCCACg                                               >  2:336376/1‑140 (MQ=14)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                               >  1:1035160/1‑140 (MQ=255)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                               >  1:1493236/1‑140 (MQ=255)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                               <  1:1193419/140‑1 (MQ=255)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                               <  1:1050464/140‑1 (MQ=255)
                                                                                           cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                               >  1:196255/1‑140 (MQ=255)
                                                                                                ggggAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCCACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCATCCGGTGCAACCGGAGCCACAGGGGc                                          >  2:2422823/1‑140 (MQ=14)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                    <  1:1175767/140‑1 (MQ=255)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCGGTGACGGTGGACCAGCCGGAGCGACCGGAGCGCCAGGAGCTACAGGTGAAGCCGGACCAGCCGGAGCTGCCGGAGCGCCGGGAGCGACggg                                    >  1:1686919/1‑140 (MQ=17)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGTGCTACAGGTGAAGACGGAGCACCCGGACCCACCGGAGCGACGGGGGcacccgg                                    >  2:452640/1‑134 (MQ=18)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                    <  1:234814/140‑1 (MQ=255)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCCACggg                                    >  1:2829874/1‑140 (MQ=255)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCCCCggg                                    >  1:2089269/1‑140 (MQ=255)
                                                                                                      gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACGGGAGCCCCAGGAGCTACCGGGGAAGCCGGAGCGCCCGGAGCCCCAGGAGCGACAGGAgaagccgg                                    >  2:2077710/1‑133 (MQ=17)
                                                                                                        tGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGCGGGCTACCCGGGACGCAGGACCATCCGGAGCGAGGGGCGCGACAGGAGCTCCCTGGGAAGCCGCACCCGCCGGACCGCCGGGGGCGCCGGGAGCGGCGGGAg                                  >  2:2962353/1‑140 (MQ=16)
                                                                                                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                          >  1:1253751/1‑140 (MQ=255)
                                                                                                                ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCggg                          <  1:409993/140‑1 (MQ=255)
                                                                                                                    accagccggcGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAag                      <  1:142944/140‑1 (MQ=255)
                                                                                                                        gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGCGCGACGGGAGCTACCGGGGAagccgg                  >  2:148488/1‑140 (MQ=255)
                                                                                                                        gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGTGCTCCCGGGGCagccgg                  >  2:2969697/1‑140 (MQ=255)
                                                                                                                        gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACCGCAGCTACCGGGGAagccgg                  >  2:1571771/1‑140 (MQ=255)
                                                                                                                         ccggaGCGCCGGGAGCCACGGGAGCTCCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTCCCGGAGCCTCAGGAGCAGGCGGAGCTCGGGGAGCGCCCGGAGCGCCGGTAGCTACCGGAGCcgccgga                 >  2:111988/1‑140 (MQ=9)
                                                                                                                         ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                 <  1:2333663/140‑1 (MQ=255)
                                                                                                                         ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                 >  1:156580/1‑140 (MQ=255)
                                                                                                                                 aCGGGAGCCACGGGAGCTCCCGGGGAAGCGGGACCCGCCGGAGCGACGGGAGCGCCCGGAGCGACCGGGGCAGCCGGCCCAGCCGCACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg         <  2:1402809/140‑1 (MQ=17)
                                                                                                                                 aCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg         <  1:939529/140‑1 (MQ=255)
                                                                                                                                        ccACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc  <  1:714317/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CCACAAGGACCACAAGGACTGCCGGGAGTCATGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCGACAGGAGCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACC  >  NZ_CP034943/607140‑607415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: