Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,637,344 T→A 100% Y44N (TAC→AAC)  EO946_RS18740 → DUF2529 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,637,3440TA100.0% 686.8 / ‑6.6 199Y44N (TAC→AAC) EO946_RS18740DUF2529 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (115/80);  minor base C (2/1);  total (118/81)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAC  >  NZ_CP034943/3637208‑3637480
                                                                                                                                        |                                                                                                                                        
ttCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAACGAATCTGACGCGATTGAAGATGGGGCTCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACg                                                                                                                                       >  2:2085833/1‑140 (MQ=255)
      tATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTa                                                                                                                                 <  2:1779038/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               >  1:266524/1‑140 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:2615937/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:346547/140‑1 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               >  1:778510/1‑140 (MQ=255)
        tGTTGAAAATATTTACGACGCAGTTAACACGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGCAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAAt                                                                                                                               <  2:1922099/140‑1 (MQ=255)
          ttGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGTGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGATAATGa                                                                                                                             >  2:2697166/1‑140 (MQ=255)
               aaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTc                                                                                                                        >  2:1095018/1‑140 (MQ=255)
               aaTATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCAGTTATTTAAACGGAGCTAATGAGATTc                                                                                                                        >  1:1755554/1‑140 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGCAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:2671373/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:417112/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:1076330/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:285590/140‑1 (MQ=255)
                   tttACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:2704036/140‑1 (MQ=255)
                      acgacgCAGTTAACAGGTATTTTTTCCCTCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGt                                                                                                                 <  2:798043/140‑1 (MQ=255)
                      acgacgCAGTTAACAGGTATTTTGTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGt                                                                                                                 <  2:2999694/140‑1 (MQ=255)
                            cAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTa                                                                                                           <  2:2960094/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  1:2875208/140‑1 (MQ=255)
                                 aaCAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAgg                                                                                                      <  2:904021/140‑1 (MQ=255)
                                     ggTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCAc                                                                                                  <  2:833682/140‑1 (MQ=255)
                                       tATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCg                                                                                                >  2:2200332/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:1757359/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:1885275/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  1:1276951/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:846038/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:2149476/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:2390166/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:250344/1‑140 (MQ=255)
                                         ttttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCAGTTCTTTAACCGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaa                                                                                              >  2:1754668/1‑140 (MQ=255)
                                          tttttCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaaa                                                                                             <  2:3112343/140‑1 (MQ=255)
                                          tttgtCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGaaa                                                                                             <  2:1447239/140‑1 (MQ=255)
                                              tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    >  1:2732240/1‑113 (MQ=255)
                                              tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAggg                                                                                                                    <  2:2732240/113‑1 (MQ=255)
                                              tCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAagcag                                                                                         >  1:3023931/1‑140 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGCTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  2:2833318/140‑1 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  1:1631758/140‑1 (MQ=255)
                                                ccGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  1:2638753/140‑1 (MQ=255)
                                                 cGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGa                                                                                      >  2:576472/1‑140 (MQ=255)
                                                  gCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGaa                                                                                     <  2:1361726/140‑1 (MQ=255)
                                                        aGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTc                                                                               >  2:2509789/1‑140 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  2:1996715/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  2:1276951/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  1:670312/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  1:2727292/140‑1 (MQ=255)
                                                            tAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCCTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           <  2:296782/140‑1 (MQ=255)
                                                                  aTCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGACCCCTTCCCATCTGTCa                                                                     >  2:2357630/1‑140 (MQ=255)
                                                                  aTCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCa                                                                     >  2:2018998/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCGAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAAAGAACCCTTCCCATCTGTCaaa                                                                   >  2:92247/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTCTGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:1209634/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:3041443/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:3086159/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:1933742/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:244809/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:186467/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  2:2274104/1‑140 (MQ=255)
                                                                    cTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCAATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTCTGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCaaa                                                                   >  1:1726602/1‑140 (MQ=255)
                                                                     tGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAg                                                                  <  2:1505454/140‑1 (MQ=255)
                                                                     tGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGATTCAGGGCGTATTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAg                                                                  <  2:299198/140‑1 (MQ=255)
                                                                        cgcgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                               >  1:13544/1‑140 (MQ=255)
                                                                        cgcgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                               >  1:2568009/1‑140 (MQ=255)
                                                                        cgcgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCt                                                               >  1:893838/1‑140 (MQ=255)
                                                                        cgcgATTGAAGATGGGGCGCGGCGGCTTGCTCAAGCGGTGATCAGCGGGCATTCCCTGTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTGAAGAGGCCACCGAACGCAGCGACCCCTTCCCATCTGTCAATGCCt                                                               >  1:2021034/1‑140 (MQ=255)
                                                                          cgATTGAAGGTGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  2:1856017/114‑1 (MQ=255)
                                                                          cgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       >  1:739774/1‑114 (MQ=255)
                                                                          cgATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCg                                                                                       <  2:739774/114‑1 (MQ=255)
                                                                           gATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTc                                                            >  2:525289/1‑140 (MQ=255)
                                                                           gATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTc                                                            >  2:2039925/1‑140 (MQ=255)
                                                                            aTTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCGTCCCATCTGTCAAAGCCTTTcc                                                           >  2:584142/1‑140 (MQ=255)
                                                                                aaGATGGGGCGCGGCTGCTTGCTCACGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCCTCTGTCAAAGCCTGTCCAGaa                                                       >  2:1396824/1‑140 (MQ=255)
                                                                                  gATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAg                                                     <  1:576472/140‑1 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  2:2163633/1‑140 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  2:2808951/1‑140 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  1:2045131/1‑140 (MQ=255)
                                                                                     ggggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTGATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAACGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAgcgc                                                  >  2:2944750/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           >  1:177495/1‑113 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCCGAAAGCGCTg                                                >  2:2506672/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGACAGCGCTg                                                >  1:1033470/1‑140 (MQ=255)
                                                                                       ggCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTg                                                >  1:2200992/1‑140 (MQ=255)
                                                                                             gctgctTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAg                                          >  1:2316783/1‑140 (MQ=255)
                                                                                                  ttgctCAAGCGGTGATCAGGGGGAATTCAATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCTAAAGCAGCGAACCGTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACa                                     <  1:2416247/140‑1 (MQ=255)
                                                                                                    gctCAAGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGa                                   <  2:2302856/140‑1 (MQ=255)
                                                                                                        aaGCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTATTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATATCTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAACGc                               >  2:666175/1‑140 (MQ=255)
                                                                                                          gCGGTGATCAGGGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCTTATTTTATGAGGCCACCTAAAGCAGCTAACCCTTCCCATGTTAAAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGa                             <  1:2895655/140‑1 (MQ=255)
                                                                                                          gCGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGa                             >  1:1145930/1‑140 (MQ=255)
                                                                                                           cGGTGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCAt                                                                           >  1:842044/1‑93 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCTTTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTAAAAGCCTTTCCAGAAAGCGCTGAGGATGTGACAGAAAGCGACa                           <  1:2462375/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGGGCATTCCATTAATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAACGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                           <  2:1565165/140‑1 (MQ=255)
                                                                                                            ggTGATCAGCGCCCATTCCATTTATTTACACGGAGCTAATGAGCTTCTGGGCGTCTTTTATGCGCCCACCGACAGCAGGGAACCCTTCCCATCTGTCAAAGCGTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACa                           <  2:1624418/140‑1 (MQ=255)
                                                                                                              tGATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTATCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAgg                         >  1:2164163/1‑140 (MQ=255)
                                                                                                               gATCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTAGGCGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCACAAAGCGCTGAGGAAGGGACACAAAGCGACAggg                        <  1:952927/140‑1 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  1:406928/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGt                       >  1:488734/1‑140 (MQ=255)
                                                                                                                aTCAGCGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCGGAGGAAGTGACAGAAAGCGACAGGGt                       >  1:2933494/1‑140 (MQ=255)
                                                                                                                     cGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                  >  1:319912/1‑140 (MQ=255)
                                                                                                                     cGGGCATTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGa                  >  1:2530088/1‑140 (MQ=255)
                                                                                                                          aTTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGttt             >  1:2963727/1‑140 (MQ=255)
                                                                                                                          aTTCCATTTATTTAAACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCAATATGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGttt             >  2:895096/1‑140 (MQ=255)
                                                                                                                                tttatttaAACGGAGCTACTGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGACAAAGCCTTTCCAGAAAGCGCTGAGGAAGTCACAGAAAGCGACAGGGTGCTGATGTTTTGCTCa       <  1:986090/140‑1 (MQ=255)
                                                                                                                                tttatttaAACGGAGCTAATGAGGTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCa       <  2:2839352/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTCACTTATGTTGAAAATATTTACGACGCAGTTAACAGGTATTTTTTCCCGCATTCAGGATAAGGAATCTGACGCGATTGAAGATGGGGCGCGGCTGCTTGCTCAAGCGGTGATCAGCGGGCATTCCATTTATTTATACGGAGCTAATGAGCTTCAGGGCGTCTTTTATGAGGCCACCGAAAGCAGCGAACCCTTCCCATCTGTCAAAGCCTTTCCAGAAAGCGCTGAGGAAGTGACAGAAAGCGACAGGGTGCTGATGTTTTGCTCAGGGAC  >  NZ_CP034943/3637208‑3637480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: