Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,936,148 T→C 5.2% S144S (TCT→TCC EO946_RS20250 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,936,1480TC 5.2% 584.7 / 14.4 173S144S (TCT→TCCEO946_RS20250aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base T (116/48);  new base C (5/4);  total (121/52)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 173 total aligned reads displayed.

TGATGCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCT  >  NZ_CP034943/3936010‑3936284
                                                                                                                                          |                                                                                                                                        
tGATGCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTg                                                                                                                                         >  2:1429013/1‑140 (MQ=255)
   tGCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGAtt                                                                                                                                      <  2:1071997/140‑1 (MQ=255)
   tGCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGAtt                                                                                                                                      <  2:1408818/140‑1 (MQ=255)
    gCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGAttt                                                                                                                                     <  1:55497/140‑1 (MQ=255)
        aCGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAATCTGTCTGATTTCACg                                                                                                                                 <  2:859350/140‑1 (MQ=255)
        aCGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACg                                                                                                                                 <  1:2583651/140‑1 (MQ=255)
        aCGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACg                                                                                                                                 <  2:2221739/140‑1 (MQ=255)
        aCGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACg                                                                                                                                 <  2:134233/140‑1 (MQ=255)
         cGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGa                                                                                                                                <  1:2852253/140‑1 (MQ=255)
          ggAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGAt                                                                                                                               <  1:1303841/140‑1 (MQ=255)
            aaTTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGa                                                                                                                             <  1:68070/140‑1 (MQ=255)
               tctcATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAaca                                                                                                                          <  2:2188341/140‑1 (MQ=255)
                      tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCac                                                                                                                   >  2:1711720/1‑139 (MQ=255)
                      tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                   >  2:2676421/1‑140 (MQ=255)
                      tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                   >  2:2532451/1‑140 (MQ=255)
                      tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                   >  2:1953752/1‑140 (MQ=255)
                         gAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAt                                                                                                                <  2:2798142/140‑1 (MQ=255)
                         gAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAt                                                                                                                <  2:848142/140‑1 (MQ=255)
                           gCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                              >  2:2428095/1‑140 (MQ=255)
                           gCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                              >  2:800851/1‑140 (MQ=255)
                           gCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                              >  2:1813616/1‑140 (MQ=255)
                                     gtcacacTCATCCTCGGCCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGt                                                                                                    <  2:1135866/134‑1 (MQ=255)
                                     aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                    >  1:635008/1‑140 (MQ=255)
                                     aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                    >  2:1350095/1‑140 (MQ=255)
                                     aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                    >  1:1192434/1‑140 (MQ=255)
                                      tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                   <  2:877702/140‑1 (MQ=255)
                                      tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                   <  2:2025755/140‑1 (MQ=255)
                                      tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                   <  2:2234213/140‑1 (MQ=255)
                                      tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                   >  2:299646/1‑140 (MQ=255)
                                      tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                   <  2:354982/140‑1 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGa                                                                                                  >  1:2524174/1‑140 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                  >  1:3043066/1‑140 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                  >  1:2607125/1‑140 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                  >  1:2491121/1‑140 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                  >  2:1182096/1‑140 (MQ=255)
                                       gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                  >  2:664361/1‑140 (MQ=255)
                                                   cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                      >  1:2979449/1‑140 (MQ=255)
                                                   cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                      >  1:654952/1‑140 (MQ=255)
                                                   cGGTCCGTGGAACTATCCGTGTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACCCAAGCAACATGGCCGCTCAGGTGATTCGAGACGCtt                                                                                      >  1:1672082/1‑140 (MQ=255)
                                                    ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                     <  2:1179373/140‑1 (MQ=255)
                                                    ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                     >  1:965432/1‑140 (MQ=255)
                                                    ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                     >  1:1819729/1‑140 (MQ=255)
                                                    ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                     >  1:2300150/1‑140 (MQ=255)
                                                    ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                     >  1:1442208/1‑140 (MQ=255)
                                                          tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCACAAGTGATTCGAGACGCTTTTGACGa                                                                               >  2:1113954/1‑140 (MQ=255)
                                                          tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGATTTTGACGa                                                                               >  1:1598910/1‑140 (MQ=255)
                                                                tATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCACAAGTGATTCGAGACGCTTTTGACGAAAaaga                                                                         >  2:2458005/1‑140 (MQ=255)
                                                                  tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                       >  1:758822/1‑140 (MQ=255)
                                                                  tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                       >  1:1816543/1‑140 (MQ=255)
                                                                  tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                       >  2:1205810/1‑140 (MQ=255)
                                                                  tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGAAAaagaag                                                                       >  2:1563986/1‑140 (MQ=255)
                                                                  tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAACTGATTCGAGACGCTTTTGACGAAAaagaag                                                                       >  1:689711/1‑140 (MQ=255)
                                                                    cGTTTATGCTGACGATGGCTCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                              >  1:286088/1‑99 (MQ=255)
                                                                    cGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                              <  2:286088/99‑1 (MQ=255)
                                                                    cGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTc                                                                     >  1:909827/1‑140 (MQ=255)
                                                                       ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                  >  1:60808/1‑140 (MQ=255)
                                                                       ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                  >  1:1538218/1‑140 (MQ=255)
                                                                       ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                  >  2:1404827/1‑140 (MQ=255)
                                                                            cTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAATTCGCGAtttt                                                             >  1:1943813/1‑140 (MQ=255)
                                                                              gACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAac                                                                                                                           <  1:2103220/76‑1 (MQ=255)
                                                                              gACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAac                                                                                                                           >  2:2103220/1‑76 (MQ=255)
                                                                              gACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGACGTCGCGAtttttt                                                           >  1:2754433/1‑139 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACCCAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                          >  2:2004983/1‑140 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                          <  2:1299190/140‑1 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                          >  1:272256/1‑140 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                          >  1:1421320/1‑140 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGGAAAAGAAGTCGCGAGTTTTGa                                                          >  2:2005064/1‑140 (MQ=255)
                                                                               aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTATGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                          <  2:2709518/140‑1 (MQ=255)
                                                                                cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                         >  1:745678/1‑140 (MQ=255)
                                                                                cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                         >  1:1180473/1‑140 (MQ=255)
                                                                                    ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGACGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                     >  1:2279857/1‑140 (MQ=255)
                                                                                    ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                     >  1:2022149/1‑140 (MQ=255)
                                                                                    ggCGCCGCTTGCCGAGTCTCTCGCAGGCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACACGGAACATTGGCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                     <  2:1873767/140‑1 (MQ=255)
                                                                                     gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATGGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgaga                                                    <  2:101752/140‑1 (MQ=255)
                                                                                         cgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGtt                                                >  1:10423/1‑140 (MQ=255)
                                                                                                   ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                   >  2:807546/1‑78 (MQ=255)
                                                                                                   ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                   <  1:807546/79‑2 (MQ=255)
                                                                                                      tctcGCAGCCGGAACCAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAGTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                   <  2:523527/140‑1 (MQ=255)
                                                                                                      tctcGCAGCCGGAAACAGCGCTCTTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                   <  2:1104546/140‑1 (MQ=255)
                                                                                                       ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                  <  2:635008/140‑1 (MQ=255)
                                                                                                       ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                  <  2:1839112/140‑1 (MQ=255)
                                                                                                        tcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAg                                 >  1:1074603/1‑140 (MQ=255)
                                                                                                             gCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGGTGAGGTGGCAACCGAGCTTCt                            >  2:2889443/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCTAGCTTCTAGATCAGCCGt                 >  1:402516/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  1:3057468/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  1:27960/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  1:3086788/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  2:423665/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  1:613729/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  2:2644474/1‑140 (MQ=255)
                                                                                                                        cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                 >  1:1153522/1‑140 (MQ=255)
                                                                                                                              tgtgAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGAcc           <  2:675338/140‑1 (MQ=255)
                                                                                                                              tgtgAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAACTACTTGATCAGCCGTTCGAcc           <  1:766531/140‑1 (MQ=255)
                                                                                                                                  aaGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAt       >  2:2716022/1‑140 (MQ=255)
                                                                                                                                   aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt      <  2:340086/140‑1 (MQ=255)
                                                                                                                                   aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt      <  2:2204573/140‑1 (MQ=255)
                                                                                                                                       gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct  >  2:2372865/1‑140 (MQ=255)
                                                                                                                                       gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct  >  2:711182/1‑140 (MQ=255)
                                                                                                                                       gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAGTttct  >  2:1308113/1‑140 (MQ=255)
                                                                                                                                       gTCTGATTTAAAGATGAACACAAGCAACATTGGCGCAAAAGAGATTAGACACGCTTTTGACGAAACAGAATTCGGGATTTTTTAAGGAGAGGTTGAGGTGCCAACCGAGCTAATAGAACAGCCGTTTGACCACAatttat  >  2:2589791/1‑138 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TGATGCAAACGGAATTCTCATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCT  >  NZ_CP034943/3936010‑3936284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: