Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,936,166 A→G 5.4% T150T (ACA→ACG EO946_RS20250 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,936,1660AG 5.4% 556.4 / 14.3 166T150T (ACA→ACGEO946_RS20250aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base A (116/41);  new base G (5/4);  total (121/45)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 170 total aligned reads displayed.

ATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCG  >  NZ_CP034943/3936029‑3936305
                                                                                                                                         |                                                                                                                                           
aTGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAg                                                                                                                                           <  1:2669278/140‑1 (MQ=255)
aTGTACGAGCCTAAAGGGATGACGCACATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAg                                                                                                                                           <  1:850722/140‑1 (MQ=255)
   tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                                        >  2:1719100/1‑140 (MQ=255)
   tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                                        >  2:1097339/1‑140 (MQ=255)
   tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                                        >  2:1953763/1‑140 (MQ=255)
   tACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCaa                                                                                                                                        >  2:2676421/1‑140 (MQ=255)
      gAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAt                                                                                                                                     <  2:2798142/140‑1 (MQ=255)
        gCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                                                   >  2:2428095/1‑140 (MQ=255)
        gCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                                                   >  2:1707905/1‑140 (MQ=255)
          cTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGcc                                                                                                                                 >  2:1018798/1‑140 (MQ=255)
                  gtcacacTCATCCTCGGCCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGt                                                                                                                         <  2:1135866/134‑1 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  1:1192434/1‑140 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  2:2437529/1‑140 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  1:638897/1‑140 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  1:635008/1‑140 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  1:2795431/1‑140 (MQ=255)
                  aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCACTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                         >  2:344004/1‑140 (MQ=255)
                   tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                        <  2:877702/140‑1 (MQ=255)
                   tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                        >  2:299646/1‑140 (MQ=255)
                   tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGAGATTGTGAAGCTGTCTGATTTCAAGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                        >  1:910293/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGa                                                                                                                       >  1:2524174/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                       >  1:2491121/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                       >  2:1179978/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                       >  1:3043066/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                       >  2:664361/1‑140 (MQ=255)
                    gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                       >  1:2607125/1‑140 (MQ=255)
                                cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                                           >  1:2979449/1‑140 (MQ=255)
                                cGGTCCGTGGAACTATCCGTGTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACCCAAGCAACATGGCCGCTCAGGTGATTCGAGACGCtt                                                                                                           >  1:1672082/1‑140 (MQ=255)
                                 ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                          >  1:1442208/1‑140 (MQ=255)
                                 ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                          >  1:1819729/1‑140 (MQ=255)
                                 ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                          >  1:2300150/1‑140 (MQ=255)
                                   tCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTg                                                                                                        >  1:3047228/1‑140 (MQ=255)
                                       tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGATTCGAGACGATTTTGACGa                                                                                                    >  2:1113899/1‑140 (MQ=255)
                                               tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                            >  2:1314224/1‑140 (MQ=255)
                                               tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                            >  2:1205810/1‑140 (MQ=255)
                                               tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                            >  1:581460/1‑140 (MQ=255)
                                               tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                            >  1:1816543/1‑140 (MQ=255)
                                               tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGAAAaagaag                                                                                            >  2:1563986/1‑140 (MQ=255)
                                                 cGTTTATGCTGACGATGGCTCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                                                   >  1:286088/1‑99 (MQ=255)
                                                 cGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTg                                                                                                                                   <  2:286088/99‑1 (MQ=255)
                                                    ttATGCTGACGATTGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                                       >  2:1404837/1‑140 (MQ=255)
                                                         cTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAATTCGCGAtttt                                                                                  >  1:1943813/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACCCAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               >  2:2004983/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               >  1:1421320/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               >  1:272256/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               >  1:1580432/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               >  1:2539780/1‑140 (MQ=255)
                                                            aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTATGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                               <  2:2709518/140‑1 (MQ=255)
                                                             cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                              >  1:745832/1‑140 (MQ=255)
                                                             cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                              >  1:1180473/1‑140 (MQ=255)
                                                             cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                              >  1:745678/1‑140 (MQ=255)
                                                                 ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGACGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                          >  1:2279857/1‑140 (MQ=255)
                                                                 ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                          <  1:1314224/140‑1 (MQ=255)
                                                                  gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAACAAGAAGTCGCGATTTTTGAAGgaga                                                                         >  2:1272833/1‑140 (MQ=255)
                                                                  gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATGGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgaga                                                                         <  2:101752/140‑1 (MQ=255)
                                                                     ccgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAACAGAAGTCGCGATTTTTGAAGGAGAGGt                                                                      >  2:2477110/1‑140 (MQ=255)
                                                                      cgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGtt                                                                     >  1:10423/1‑140 (MQ=255)
                                                                                ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                        >  2:807546/1‑78 (MQ=255)
                                                                                ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                        >  2:807524/1‑78 (MQ=255)
                                                                                ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                        <  1:807546/79‑2 (MQ=255)
                                                                                  ctctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAAcc                                                         >  2:766531/1‑140 (MQ=255)
                                                                                   tctcGCAGCCGGTCACAGCGCGATTGAGCCGCTGTCTGATTTCACCCAGCCCACACGCAACCTTGCCGCACAACCGCTCAGAGACCCTTTTGACGCAACAGCAGTCGCGATTTTGCAAGGAGAGGTTGAGGTGGCAACCg                                                        <  2:2270679/140‑1 (MQ=255)
                                                                                   tctcGCAGCCGGAACCAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAGTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                                        <  2:523527/140‑1 (MQ=255)
                                                                                    ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                       <  2:1839112/140‑1 (MQ=255)
                                                                                    ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                       <  2:635008/140‑1 (MQ=255)
                                                                                    ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                       <  2:851435/140‑1 (MQ=255)
                                                                                     tcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAg                                                      >  1:1074603/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGCCGCTTTTGACGAAAAAGACGTCGCGATTTTTGTAGGAGAGGTTGTGGTGGCAACCGAGCTTCTCGATCAGCCGt                                      >  2:2875525/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGACGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:1860710/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCTAGCTTCTAGATCAGCCGt                                      >  1:402516/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:1153522/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:1138528/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:613729/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:2644474/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:423665/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:712143/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:1944829/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:675338/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:27960/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:1403378/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  1:3086788/1‑140 (MQ=255)
                                                                                                     cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTGTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                      >  2:2011159/1‑140 (MQ=255)
                                                                                                           tgtgAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAACTACTTGATCAGCCGTTCGAcc                                <  1:766531/140‑1 (MQ=255)
                                                                                                               aaGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAt                            >  2:2716022/1‑140 (MQ=255)
                                                                                                                aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                           <  2:2176478/140‑1 (MQ=255)
                                                                                                                aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                           <  2:2204573/140‑1 (MQ=255)
                                                                                                                aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                           <  2:340086/140‑1 (MQ=255)
                                                                                                                    gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                       >  2:2228596/1‑140 (MQ=255)
                                                                                                                    gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                       >  2:696859/1‑140 (MQ=255)
                                                                                                                    gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                       >  2:711182/1‑140 (MQ=255)
                                                                                                                    gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                       >  2:1306417/1‑140 (MQ=255)
                                                                                                                    gTCTGATTTAAAGATGAACACAAGCAACATTGGCGCAAAAGAGATTAGACACGCTTTTGACGAAACAGAATTCGGGATTTTTTAAGGAGAGGTTGAGGTGCCAACCGAGCTAATAGAACAGCCGTTTGACCACAatttat                       >  2:2589791/1‑138 (MQ=255)
                                                                                                                           ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                <  1:1179978/140‑1 (MQ=255)
                                                                                                                           ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                <  1:1953763/140‑1 (MQ=255)
                                                                                                                           ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                <  1:1719100/140‑1 (MQ=255)
                                                                                                                           ttCACGATGAACACAAGCAACAGTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                <  1:1953752/140‑1 (MQ=255)
                                                                                                                               cGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGc            >  1:1610316/1‑140 (MQ=255)
                                                                                                                                   gAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACaa        >  2:618185/1‑140 (MQ=255)
                                                                                                                                      cacaAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATg     >  2:2569828/1‑140 (MQ=255)
                                                                                                                                         aaGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCg  >  2:1324932/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATGTACGAGCCTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCG  >  NZ_CP034943/3936029‑3936305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: