Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,936,178 C→T 5.4% A154A (GCC→GCT EO946_RS20250 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,936,1780CT 5.4% 555.5 / 14.1 166A154A (GCC→GCTEO946_RS20250aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (116/40);  new base T (5/4);  total (121/45)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 173 total aligned reads displayed.

CTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCA  >  NZ_CP034943/3936039‑3936317
                                                                                                                                           |                                                                                                                                           
cTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGcc                                                                                                                                             >  2:1018798/1‑140 (MQ=255)
        gtcacacTCATCCTCGGCCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGt                                                                                                                                     <  2:1135866/134‑1 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  2:2437529/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  1:1192434/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  2:1350095/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  1:638897/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  1:2795431/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  1:1839112/1‑140 (MQ=255)
        aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCACTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                     >  2:344004/1‑140 (MQ=255)
         tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                    <  2:877702/140‑1 (MQ=255)
         tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                    <  2:354982/140‑1 (MQ=255)
         tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                    <  2:2234213/140‑1 (MQ=255)
          gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGa                                                                                                                                   >  1:2524174/1‑140 (MQ=255)
          gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                   >  2:664361/1‑140 (MQ=255)
          gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                   >  2:1182096/1‑140 (MQ=255)
          gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                   >  1:3043066/1‑140 (MQ=255)
          gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                   >  1:2607125/1‑140 (MQ=255)
                      cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                                                       >  1:654952/1‑140 (MQ=255)
                      cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                                                       >  1:2979449/1‑140 (MQ=255)
                       ggTCCGTGGAACTATGCGTTTATGCTGTCGATGGCGACGCTTGCCGCTTCTCTCGCATGCGGAAAAAGCGCGATTGTGTTTCTGTCTGATTTCACGTTTAACACAAGCAACATTGCAGCAAAAGTGATTCGAGACGCttt                                                                                                                      <  1:2962094/140‑1 (MQ=255)
                       ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                      >  1:1819729/1‑140 (MQ=255)
                       ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                      >  1:1442208/1‑140 (MQ=255)
                       ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                      <  2:1179373/140‑1 (MQ=255)
                       ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                      >  1:965432/1‑140 (MQ=255)
                       ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                      >  1:2300150/1‑140 (MQ=255)
                         tCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTg                                                                                                                    >  1:3047228/1‑140 (MQ=255)
                             tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGATTCGAGACGATTTTGACGa                                                                                                                >  2:1113899/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                        >  1:758822/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                        >  2:1205810/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                        >  1:615128/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                        >  1:581460/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGAAAaagaag                                                                                                        >  2:1563986/1‑140 (MQ=255)
                                     tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAACTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                        >  1:689711/1‑140 (MQ=255)
                                       cGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTc                                                                                                      >  1:909827/1‑140 (MQ=255)
                                          ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                                                   >  2:1404827/1‑140 (MQ=255)
                                          ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                                                   >  1:60808/1‑140 (MQ=255)
                                               cTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGAtttt                                                                                              >  2:613878/1‑140 (MQ=255)
                                                 gACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGACGTCGCGAtttttt                                                                                            >  1:2754433/1‑139 (MQ=255)
                                                  aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                           <  2:1299190/140‑1 (MQ=255)
                                                  aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                           >  1:1580432/1‑140 (MQ=255)
                                                  aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                           >  1:2375595/1‑140 (MQ=255)
                                                  aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGGAAAAGAAGTCGCGAGTTTTGa                                                                                           >  2:2005064/1‑140 (MQ=255)
                                                   cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                          >  1:1180473/1‑140 (MQ=255)
                                                   cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                          >  1:745832/1‑140 (MQ=255)
                                                   cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                          >  1:1953401/1‑140 (MQ=255)
                                                       ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGACGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                      >  1:2279857/1‑140 (MQ=255)
                                                       ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                      <  1:1314224/140‑1 (MQ=255)
                                                       ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                      >  1:2022149/1‑140 (MQ=255)
                                                       ggCGCCGCGTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                      <  2:284131/140‑1 (MQ=255)
                                                        gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgaga                                                                                     <  1:2840736/140‑1 (MQ=255)
                                                           ccgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAACAGAAGTCGCGATTTTTGAAGGAGAGGt                                                                                  >  2:2477110/1‑140 (MQ=255)
                                                            cgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGtt                                                                                 >  1:10423/1‑140 (MQ=255)
                                                                      ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                                    <  1:807524/79‑2 (MQ=255)
                                                                      ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                                    >  2:807524/1‑78 (MQ=255)
                                                                        ctctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAAcc                                                                     >  2:766531/1‑140 (MQ=255)
                                                                         tctcGCAGCCGGAACCAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACAGTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                                                    <  2:523527/140‑1 (MQ=255)
                                                                         tctcGCAGCCGGAAACAGCGCTCTTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                                                    <  2:1104546/140‑1 (MQ=255)
                                                                          ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                   <  1:3094594/140‑1 (MQ=255)
                                                                          ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                   <  2:851435/140‑1 (MQ=255)
                                                                          ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                   <  2:638897/140‑1 (MQ=255)
                                                                           tcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAg                                                                  >  1:1074603/1‑140 (MQ=255)
                                                                                gCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGGTGAGGTGGCAACCGAGCTTCt                                                             >  2:2889443/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGACGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                  >  2:1860710/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTATATCAGCCGt                                                  >  2:2702225/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                  >  1:3057468/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                  >  1:1153522/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                  >  2:1944829/1‑140 (MQ=255)
                                                                                           cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTGTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                  >  2:2011159/1‑140 (MQ=255)
                                                                                                     aaGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAt                                        >  2:2716022/1‑140 (MQ=255)
                                                                                                     aaGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGAGTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCCCAt                                        >  2:2622331/1‑140 (MQ=255)
                                                                                                     aaGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAACTACTTGATCAGCCGTTCGACCatat                                        >  1:143893/1‑140 (MQ=255)
                                                                                                          gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                   >  2:2372865/1‑140 (MQ=255)
                                                                                                          gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                   >  2:696859/1‑140 (MQ=255)
                                                                                                          gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                   >  2:2228596/1‑140 (MQ=255)
                                                                                                          gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                   >  2:2671584/1‑140 (MQ=255)
                                                                                                          gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAGTttct                                   >  2:1308113/1‑140 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:1719100/140‑1 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:1711720/140‑1 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:2676421/140‑1 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:1953763/140‑1 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:1179978/140‑1 (MQ=255)
                                                                                                                 ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                            <  1:2532451/140‑1 (MQ=255)
                                                                                                                     cGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGAcc                                            <  1:717327/120‑1 (MQ=255)
                                                                                                                     cGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGc                        >  1:1610316/1‑140 (MQ=255)
                                                                                                                            cacaAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATg                 >  2:2569828/1‑140 (MQ=255)
                                                                                                                             acaAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGt                <  2:3086788/140‑1 (MQ=255)
                                                                                                                               aaGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCg              >  2:1324932/1‑140 (MQ=255)
                                                                                                                                aGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCgg             >  1:997746/1‑140 (MQ=255)
                                                                                                                                    aCATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGacaa         >  1:1794838/1‑140 (MQ=255)
                                                                                                                                    aCATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGaaaa         >  1:2570331/1‑140 (MQ=255)
                                                                                                                                    aCATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGaaaa         >  2:2088882/1‑140 (MQ=255)
                                                                                                                                       ttGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAAtt      >  2:468667/1‑140 (MQ=255)
                                                                                                                                       ttGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAAtt      >  2:711989/1‑140 (MQ=255)
                                                                                                                                         gCCGCAACAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGt    <  2:2198474/140‑1 (MQ=255)
                                                                                                                                         gCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGt    <  1:299646/140‑1 (MQ=255)
                                                                                                                                          ccGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTc   >  2:540321/1‑140 (MQ=255)
                                                                                                                                           cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa  >  2:623773/1‑140 (MQ=255)
                                                                                                                                           cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa  >  2:1447673/1‑140 (MQ=255)
                                                                                                                                           cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa  >  2:3012008/1‑140 (MQ=255)
                                                                                                                                           cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa  >  2:2976729/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTAAAGGGATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCA  >  NZ_CP034943/3936039‑3936317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: