Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,936,181 A→T 5.5% A155A (GCA→GCT EO946_RS20250 → aldehyde dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,936,1810AT 5.5% 598.3 / 14.9 172A155A (GCA→GCTEO946_RS20250aldehyde dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base A (120/42);  new base T (6/4);  total (126/46)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 173 total aligned reads displayed.

ATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCATGA  >  NZ_CP034943/3936047‑3936320
                                                                                                                                      |                                                                                                                                           
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  2:1350095/1‑140 (MQ=255)
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  1:2795431/1‑140 (MQ=255)
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  1:638897/1‑140 (MQ=255)
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  2:489453/1‑140 (MQ=255)
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  1:1839112/1‑140 (MQ=255)
aTGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGt                                                                                                                                        >  1:1192434/1‑140 (MQ=255)
 tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                       >  2:299646/1‑140 (MQ=255)
 tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                       <  2:354982/140‑1 (MQ=255)
 tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                       <  2:2025755/140‑1 (MQ=255)
 tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                       <  2:2234213/140‑1 (MQ=255)
 tGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTg                                                                                                                                       <  2:877702/140‑1 (MQ=255)
  gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGa                                                                                                                                      >  1:2524174/1‑140 (MQ=255)
  gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                      >  1:3043066/1‑140 (MQ=255)
  gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                      >  1:2607125/1‑140 (MQ=255)
  gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                      >  2:1179978/1‑140 (MQ=255)
  gACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGa                                                                                                                                      >  2:664361/1‑140 (MQ=255)
              cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                                                          >  1:2979449/1‑140 (MQ=255)
              cGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCtt                                                                                                                          >  1:654952/1‑140 (MQ=255)
               ggTCCGTGGAACTATGCGTTTATGCTGTCGATGGCGACGCTTGCCGCTTCTCTCGCATGCGGAAAAAGCGCGATTGTGTTTCTGTCTGATTTCACGTTTAACACAAGCAACATTGCAGCAAAAGTGATTCGAGACGCttt                                                                                                                         <  1:2962094/140‑1 (MQ=255)
               ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                         >  1:1442208/1‑140 (MQ=255)
               ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                         >  1:1819729/1‑140 (MQ=255)
               ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                         >  1:2300150/1‑140 (MQ=255)
               ggTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCttt                                                                                                                         >  1:965432/1‑140 (MQ=255)
                 tCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTg                                                                                                                       >  1:3047228/1‑140 (MQ=255)
                     tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCACCATTGCCGCAAAAGTGATTCGAGACGATTTTGACGa                                                                                                                   >  2:1113899/1‑140 (MQ=255)
                     tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCACAAGTGATTCGAGACGCTTTTGACGa                                                                                                                   >  2:1113954/1‑140 (MQ=255)
                     tGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGa                                                                                                                   >  2:2793085/1‑140 (MQ=255)
                           tATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCACAAGTGATTCGAGACGCTTTTGACGAAAaaga                                                                                                             >  2:2458005/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  1:615128/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  1:581460/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  2:1314224/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  1:758822/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  2:1205810/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCGTTTGACGAAAaagaag                                                                                                           >  2:1563986/1‑140 (MQ=255)
                             tCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAACTGATTCGAGACGCTTTTGACGAAAaagaag                                                                                                           >  1:689711/1‑140 (MQ=255)
                               cGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTc                                                                                                         >  1:909827/1‑140 (MQ=255)
                                  ttATGCTGACGATTGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                                                      >  2:1404837/1‑140 (MQ=255)
                                  ttATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTcgcg                                                                                                      >  1:60808/1‑140 (MQ=255)
                                       cTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGAtttt                                                                                                 >  2:613878/1‑140 (MQ=255)
                                         gACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGACGTCGCGAtttttt                                                                                               >  1:2754433/1‑139 (MQ=255)
                                          aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACCCAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                              >  2:2004983/1‑140 (MQ=255)
                                          aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                              >  1:2375595/1‑140 (MQ=255)
                                          aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                              >  1:1580432/1‑140 (MQ=255)
                                          aCGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTATGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGa                                                                                              <  2:2709518/140‑1 (MQ=255)
                                           cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                             >  1:745832/1‑140 (MQ=255)
                                           cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                             >  1:1953401/1‑140 (MQ=255)
                                           cGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGaa                                                                                             >  1:1180473/1‑140 (MQ=255)
                                               ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGACGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                         >  1:2279857/1‑140 (MQ=255)
                                               ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                         >  1:2022149/1‑140 (MQ=255)
                                               ggCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                         <  1:1314224/140‑1 (MQ=255)
                                               ggCGCCGCTTGCCGAGTCTCTCGCAGGCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACACGGAACATTGGCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgag                                                                                         <  2:1873767/140‑1 (MQ=255)
                                                gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAACAAGAAGTCGCGATTTTTGAAGgaga                                                                                        >  2:1272833/1‑140 (MQ=255)
                                                gcgcCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGgaga                                                                                        <  1:2840736/140‑1 (MQ=255)
                                                    cgcttgccgcttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGtt                                                                                    >  1:10423/1‑140 (MQ=255)
                                                              ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                                       >  2:807524/1‑78 (MQ=255)
                                                              ttCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGta                                                                                                                                       <  1:807524/79‑2 (MQ=255)
                                                                ctctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAAcc                                                                        >  2:766531/1‑140 (MQ=255)
                                                                 tctcGCAGCCGGTCACAGCGCGATTGAGCCGCTGTCTGATTTCACCCAGCCCACACGCAACCTTGCCGCACAACCGCTCAGAGACCCTTTTGACGCAACAGCAGTCGCGATTTTGCAAGGAGAGGTTGAGGTGGCAACCg                                                                       <  2:2270679/140‑1 (MQ=255)
                                                                  ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                      <  2:638897/140‑1 (MQ=255)
                                                                  ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                      <  2:851435/140‑1 (MQ=255)
                                                                  ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                      <  2:1839112/140‑1 (MQ=255)
                                                                  ctcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGa                                                                      <  1:3094594/140‑1 (MQ=255)
                                                                   tcGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAg                                                                     >  1:1074603/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGCCGCTTTTGACGAAAAAGACGTCGCGATTTTTGTAGGAGAGGTTGTGGTGGCAACCGAGCTTCTCGATCAGCCGt                                                     >  2:2875525/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTATATCAGCCGt                                                     >  2:2702225/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  2:1403378/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  2:1138528/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  1:1153522/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  2:423665/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  1:3057468/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  2:2644474/1‑140 (MQ=255)
                                                                                   cgcgATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTGTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGt                                                     >  2:2011159/1‑140 (MQ=255)
                                                                                             aaGCTGTCCGATTTCACGATGAACACGAGCAACATTGCTGCTAAAGTAATAAGAGACGCTTTTGATGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAACTACTTGATCAGCCGTTCGACCatat                                           >  1:143893/1‑140 (MQ=255)
                                                                                              aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                                          <  2:2176478/140‑1 (MQ=255)
                                                                                              aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                                          <  2:2204573/140‑1 (MQ=255)
                                                                                              aGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACAtt                                          <  2:340086/140‑1 (MQ=255)
                                                                                                  gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                      >  2:1306417/1‑140 (MQ=255)
                                                                                                  gTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTttct                                      >  2:696859/1‑140 (MQ=255)
                                                                                                  gTCTGATTTAAAGATGAACACAAGCAACATTGGCGCAAAAGAGATTAGACACGCTTTTGACGAAACAGAATTCGGGATTTTTTAAGGAGAGGTTGAGGTGCCAACCGAGCTAATAGAACAGCCGTTTGACCACAatttat                                      >  2:2589791/1‑138 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:2676421/140‑1 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:1179978/140‑1 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:2532451/140‑1 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:1711720/140‑1 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:1953763/140‑1 (MQ=255)
                                                                                                         ttCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTgg                               <  1:1719100/140‑1 (MQ=255)
                                                                                                             cGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGAcc                                               <  1:717327/120‑1 (MQ=255)
                                                                                                             cGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGc                           >  1:1610316/1‑140 (MQ=255)
                                                                                                                    cacaAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATg                    >  2:2569828/1‑140 (MQ=255)
                                                                                                                       aaGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCg                 >  2:1052104/1‑140 (MQ=255)
                                                                                                                        aGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCgg                >  1:997746/1‑140 (MQ=255)
                                                                                                                            aCATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGacaa            >  1:1794838/1‑140 (MQ=255)
                                                                                                                            aCATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGaaaa            >  1:2570331/1‑140 (MQ=255)
                                                                                                                               ttGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAAtt         >  2:711989/1‑140 (MQ=255)
                                                                                                                                 gCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCg                                                                       <  2:3051952/76‑1 (MQ=255)
                                                                                                                                 gCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGt       <  1:299646/140‑1 (MQ=255)
                                                                                                                                  ccGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTc      >  2:540321/1‑140 (MQ=255)
                                                                                                                                  ccGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTc      <  2:2432047/140‑1 (MQ=255)
                                                                                                                                   cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa     >  2:623773/1‑140 (MQ=255)
                                                                                                                                   cGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCa     >  2:2976729/1‑140 (MQ=255)
                                                                                                                                      aaaaGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCATGa  <  2:551345/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                           
ATGACGCTCATCCTCGGTCCGTGGAACTATCCGTTTATGCTGACGATGGCGCCGCTTGCCGCTTCTCTCGCAGCCGGAAACAGCGCGATTGTGAAGCTGTCTGATTTCACGATGAACACAAGCAACATTGCCGCAAAAGTGATTCGAGACGCTTTTGACGAAAAAGAAGTCGCGATTTTTGAAGGAGAGGTTGAGGTGGCAACCGAGCTTCTAGATCAGCCGTTTGACCACATTTTCTTCACTGGAAGCACAAATGTCGGAAAAATTGTCATGA  >  NZ_CP034943/3936047‑3936320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: