Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,992,887 G→A 13.6% T258I (ACA→ATA)  walK ← cell wall metabolism sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,992,8870GA13.6% 650.4 / 74.5 235T258I (ACA→ATA) walKcell wall metabolism sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base G (93/110);  new base A (18/14);  total (111/124)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 235 total aligned reads displayed.

AGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTC  >  NZ_CP034943/3992750‑3993021
                                                                                                                                         |                                                                                                                                      
aGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTc                                                                                                                                      <  1:590542/140‑1 (MQ=255)
 ggCTGTTTCACGTGCAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGCTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCa                                                                                                                                     <  2:1774887/140‑1 (MQ=255)
 ggCTGTGTCACGTGACACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCa                                                                                                                                     <  2:1358615/140‑1 (MQ=255)
     gTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGc                                                                                                                                 >  2:1340586/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                                                                                                                                >  2:1531737/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                                                                                                                                <  2:2281461/140‑1 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                                                                                                                                >  1:31239/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                                                                                                                                >  1:1602918/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                                                                                                                                >  1:2024384/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGcc                                                                                                                                >  1:963719/1‑140 (MQ=255)
      tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGcc                                                                                                                                >  1:2692606/1‑140 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                                                                                                                            <  2:2941683/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                                                                                                                            <  2:1867983/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                                                                                                                            <  2:2148619/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                                                                                                                            <  1:1481033/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                                                                                                                            <  2:2365367/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCCggg                                                                                                                            <  2:1550974/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTggg                                                                                                                            <  1:850166/140‑1 (MQ=255)
          aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTggg                                                                                                                            <  1:1316701/140‑1 (MQ=255)
           cGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAAGAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGc                                                                                                                           <  2:1679048/140‑1 (MQ=255)
              gAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATc                                                                                                                        >  2:1156530/1‑140 (MQ=255)
                aaCGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCtt                                                                                                                      >  2:1185846/1‑140 (MQ=255)
                    ttCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCGGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAg                                                                                                                  >  1:1739427/1‑140 (MQ=255)
                    ttCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAg                                                                                                                  >  1:2466614/1‑140 (MQ=255)
                    ttCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTggg                                                                                                                            <  1:2946640/130‑1 (MQ=255)
                     tCAGCAGCTCCCGCGCCGGGCTGTTTAAGAGAAAAATTGCCACGATGCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCATTCTGTCATCGCCTGTGCATCTTAAAGc                                                                                                                 >  2:217308/1‑140 (MQ=255)
                     tCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGc                                                                                                                 >  2:1164927/1‑140 (MQ=255)
                      cagcagCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  1:1793479/140‑1 (MQ=255)
                      cagcagCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  1:94558/140‑1 (MQ=255)
                      cagcagCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  2:461811/140‑1 (MQ=255)
                        gcagcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct                                                                                                              <  2:2079281/140‑1 (MQ=255)
                        gcagcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct                                                                                                              <  1:1150939/140‑1 (MQ=255)
                        gcagcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct                                                                                                              <  1:255440/140‑1 (MQ=255)
                        gcagcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct                                                                                                              <  2:2925569/140‑1 (MQ=255)
                            cTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCg                                                                                                          >  2:553690/1‑140 (MQ=255)
                            cTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCg                                                                                                          >  1:706372/1‑140 (MQ=255)
                            cTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCg                                                                                                          >  1:2199732/1‑140 (MQ=255)
                               cAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTa                                                                                                       <  1:1544752/140‑1 (MQ=255)
                                     cGGGCTGTTTAAGAGAATAATGGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGAt                                                                                                 <  2:2060688/140‑1 (MQ=255)
                                      gggCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGAtt                                                                                                >  2:1613673/1‑140 (MQ=255)
                                        gCTGTTGAAGAGAATAAGTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATTACAGAGGCAAGCGTTCTGCGCTCTCCTTCTGTCATCGCCGGGGCATCTTCCAGCTCTCTCGTTAAGTGATTaa                                                                                              <  2:280126/140‑1 (MQ=255)
                                         cTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTaaa                                                                                             <  2:1589687/140‑1 (MQ=255)
                                         cTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTaaa                                                                                             <  2:637245/140‑1 (MQ=255)
                                           gTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACg                                                                                           <  2:2421079/140‑1 (MQ=255)
                                                    aataatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  2:1414374/110‑1 (MQ=255)
                                                    aataatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  2:3091724/110‑1 (MQ=255)
                                                    aataatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCaa                                                                                  >  1:2248719/1‑140 (MQ=255)
                                                    aataatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCaa                                                                                  >  2:2867509/1‑140 (MQ=255)
                                                    aataatTGCCCCGGTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                >  1:3091724/1‑110 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATGACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  1:1962488/108‑1 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATGACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                >  2:1962488/1‑108 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct                                                                                                                <  1:55053/108‑1 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  2:2242321/1‑140 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  2:812962/1‑140 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  2:2211875/1‑140 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  1:2003683/1‑140 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  1:1882078/1‑140 (MQ=255)
                                                      taatTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGc                                                                                >  1:2119774/1‑140 (MQ=255)
                                                        atTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTg                                                                              <  1:1471043/140‑1 (MQ=255)
                                                          tGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGcc                                                                            <  1:2642239/140‑1 (MQ=255)
                                                            ccccGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCa                                                                          >  1:22950/1‑140 (MQ=255)
                                                              ccGTTTCGTTTTGTGGCGATAACGCCGTCTGTCATATAGGCAAGAACAGAGGCAAGCTTTCTGCGCTCTCCGTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAAt                                                                        <  2:1087039/140‑1 (MQ=255)
                                                                   tCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCACGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  1:2783268/140‑1 (MQ=255)
                                                                   tCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  2:3073429/140‑1 (MQ=255)
                                                                   tCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  1:1696454/140‑1 (MQ=255)
                                                                   tCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  1:761697/140‑1 (MQ=255)
                                                                   tCGGTGTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  2:2499869/140‑1 (MQ=255)
                                                                      gTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTcatcat                                                                >  2:39311/1‑140 (MQ=255)
                                                                      gTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCAGCCCAATTTcatcat                                                                >  1:1534249/1‑140 (MQ=255)
                                                                           tgGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCg                                                           <  1:1362879/140‑1 (MQ=255)
                                                                           tgGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCg                                                           >  1:65190/1‑140 (MQ=255)
                                                                           tgGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGACTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCACGCTGCCCAATTTCATCATGCCCg                                                           >  2:1258822/1‑140 (MQ=255)
                                                                            ggCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGt                                                          <  2:93268/140‑1 (MQ=255)
                                                                            ggCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGt                                                          <  2:1540691/140‑1 (MQ=255)
                                                                                aTAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACtt                                                      >  1:998883/1‑140 (MQ=255)
                                                                                         tCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCtt                                             >  2:2962648/1‑140 (MQ=255)
                                                                                              cATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCg                                        <  1:1003479/140‑1 (MQ=255)
                                                                                                       aaTAACAGAGGCACGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTAGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTcc                               >  1:142555/1‑140 (MQ=255)
                                                                                                       aaTAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTaa                                                                                                      <  2:787684/69‑1 (MQ=255)
                                                                                                       aaTAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTcc                               >  2:728332/1‑140 (MQ=255)
                                                                                                         tAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCt                             <  1:1159734/140‑1 (MQ=255)
                                                                                                         tAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCt                             <  2:30561/140‑1 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCCTCGCCTGGGCATCTTCAAGCTCTCGCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGACATTCCCTTTCGCa                       >  2:880767/1‑140 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCa                       >  1:422819/1‑140 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCa                       >  1:808956/1‑140 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCa                       >  2:782872/1‑140 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCa                       >  1:1243182/1‑140 (MQ=255)
                                                                                                               agGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCGTCAAGCTCTCTCGTAAAGTGATTAAACGGAGTGGCAACCTGCCCAATTTCAACATGCCAGTACTTTTTAACCTTACTCTAGAACTTCCCTTTCGCa                       >  2:352298/1‑140 (MQ=255)
                                                                                                                ggCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCaa                      <  2:2672417/140‑1 (MQ=255)
                                                                                                                ggCAAGCTTTCTGCGCTCTCCTTCTATCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCaa                      <  2:1071756/140‑1 (MQ=255)
                                                                                                                 gCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATTCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCaat                     >  1:139715/1‑139 (MQ=255)
                                                                                                                 gCAAGCTTTCTGCGCTATCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGAAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACATTCCTCGAGAAATTCCCTTTATCAAg                     >  1:880814/1‑140 (MQ=255)
                                                                                                                          cTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGc            <  1:2867509/140‑1 (MQ=255)
                                                                                                                          cTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGc            <  1:1183193/140‑1 (MQ=255)
                                                                                                                           tGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   <  2:3003883/84‑1 (MQ=255)
                                                                                                                           tGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTtcat                                                                   >  1:3003883/1‑84 (MQ=255)
                                                                                                                              gcTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGt        <  1:1509933/140‑1 (MQ=255)
                                                                                                                               ctctCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTaa                                                 >  1:2332917/1‑98 (MQ=255)
                                                                                                                                tctcCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCTTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGttt      >  2:3025194/1‑140 (MQ=255)
                                                                                                                                    cTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTtctc  <  2:2109496/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
AGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCTCTCGTTAAGTGATTAAACGTAGTGGCAAGCTGCCCAATTTCATCATGCCCGTACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGTTTTCTC  >  NZ_CP034943/3992750‑3993021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: