Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 4,040,429 C→A 7.5% R197L (CGT→CTT)  mnmG ← tRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349434,040,4290CA 7.5% 604.5 / 10.5 244R197L (CGT→CTT) mnmGtRNA uridine‑5‑carboxymethylaminomethyl(34) synthesis enzyme MnmG
Reads supporting (aligned to +/- strand):  ref base C (83/136);  new base A (23/2);  total (106/138)
Fisher's exact test for biased strand distribution p-value = 1.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

CCAGCAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGC  >  NZ_CP034943/4040290‑4040568
                                                                                                                                           |                                                                                                                                           
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                             >  2:2775445/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTGTTAAaac                                                                                                                                             >  1:1077201/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGTACCGGTTTTAAaac                                                                                                                                             >  1:2612704/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTGTGACGCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                             >  1:178893/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                             >  2:1624756/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATAGTCACCAGGCTTAATTTCCGTTTTGCTGTAATCAATCGATTCGATTTTTACGCGCGGCGGAGGACCTGTTTTAAaac                                                                                                                                             >  1:2155479/1‑140 (MQ=255)
ccagcAAGGCAGCTGATCTGTAATATATTACACTCTTTCATATTAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTCATCCATCGAGTCGCTTTTGCCTCGAGGCGGCGTACCTGTTTTAAaac                                                                                                                                             >  2:2528563/1‑140 (MQ=255)
    cAAGGCAGCTGATCTTTAATATATTCCACTGTTTAATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                         <  2:2248940/140‑1 (MQ=255)
    cAAGGCAGCTGATCTGTAATATATTCCAGTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                         <  2:2344585/140‑1 (MQ=255)
    cAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                         <  2:2973691/140‑1 (MQ=255)
    cAAGGAAGCTGATCTGTAATATATTCCACTTTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGATGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAaacgaac                                                                                                                                         <  2:2723405/140‑1 (MQ=255)
      agtcAGCTTATCTGTAATATATTCCAATGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGATGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAacgaacga                                                                                                                                       <  2:2824940/137‑1 (MQ=255)
      aGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAacgaacga                                                                                                                                       <  1:560370/140‑1 (MQ=255)
      aGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAacgaacga                                                                                                                                       <  2:2448471/140‑1 (MQ=255)
      aGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAacgaacga                                                                                                                                       <  2:624139/140‑1 (MQ=255)
         cAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGCGGt                                                                                                                                    >  2:3058509/1‑140 (MQ=255)
            cggATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCTCTTTTGACGCGAGGCGGCGTACCAGTTTTAAAAACAACGAGGTCaa                                                                                                                                 >  1:2507238/3‑140 (MQ=255)
                    tAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGt                                                                                                                         <  2:3091498/140‑1 (MQ=255)
                    tAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGGACCGGGTTTAAAACGCACGCGGTCACAGcgccgg                                                                                                                         >  2:1716673/1‑135 (MQ=255)
                       tatatTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTtcc                                                                                                                      <  1:2775445/140‑1 (MQ=255)
                       tatatTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTtcc                                                                                                                      <  1:2407643/140‑1 (MQ=255)
                         tatTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGATTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAAACATGTCAAAGCCCAGTtgctc                                                                                                                    >  1:1442663/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTGACTGTTTTAAAACCAAAGAGGTCAAAGCCCACTTCCTCCaa                                                                                                                 >  2:817493/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAAAGAGGTCAAAGCCCAGTTCCTCCaa                                                                                                                 >  2:143695/1‑140 (MQ=255)
                            tCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGGACCTGTTTTAAAACGAAAGAGGTCAAAGCCCAGTTCCTCCaa                                                                                                                 >  2:1024844/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTTAAACGAACGAGGTCAAATCCCAGTTCCTCCAAAt                                                                                                               >  1:2303000/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  2:1381267/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               <  2:870644/140‑1 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               <  1:2724198/140‑1 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  2:1455677/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  1:204762/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGGACCTGTTTTTAAACGAACGATGTCAAAGCCCAGTTCCTCCAAAt                                                                                                               >  1:2924753/1‑140 (MQ=255)
                              cACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGGTTGACGCGCGGCGGCGTACCAGGTTTAAAACGTACGAGGGCAAATCCCAGTTCCACCAAAt                                                                                                               >  2:317429/1‑140 (MQ=255)
                                 tGTTTCATATGAGAACGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGGTGTAAAACCAACGAGGTCAAAGCCCCGTGCCTCCAAATGcg                                                                                                            >  1:1668657/1‑139 (MQ=255)
                                 tGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTCTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCt                                                                                                            >  1:837686/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCCCGGCGGGGTACCTGTTTTAAAAAGACAGACGTCAAAGCCCAGGTCGTGCAACTGCGCa                                                                                                          >  1:85414/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAATCCCAGTTCCTCCAAATGCTCa                                                                                                          >  2:2182632/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTGCTCCAAATGCTCa                                                                                                          >  2:267965/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCa                                                                                                          >  2:522347/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGTACCTGTTTTTAAACGAACGAGGTAAAATCCCACTTCCTCCAAATGCTCa                                                                                                          >  1:1988365/1‑140 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGAAGCGAGGCGGCGTACCTGTTTTAAAACGTACGAGGTCAAATCCCAGTTGCTCCCAATGcgaa                                                                                                          >  1:3037330/1‑137 (MQ=255)
                                   tttCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCCTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCa                                                                                                          >  1:1231549/1‑140 (MQ=255)
                                   tttCATATGACAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCATCCAAAAGCTCa                                                                                                          >  2:2393081/1‑140 (MQ=255)
                                     tCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGTCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGa                                                                                                        <  2:2859606/140‑1 (MQ=255)
                                     tCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGCTCCTCCAAATGCTCAGa                                                                                                        <  2:2578729/140‑1 (MQ=255)
                                     tCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTAACTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGa                                                                                                        <  1:387335/140‑1 (MQ=255)
                                     tCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCGGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCACATGCTCAGa                                                                                                        <  1:377109/140‑1 (MQ=255)
                                        tatGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCGAGGCGGCGTACCTGTTTTAAAAAGAAAGAGGTCAAAGCCCAGTTCCTGCAAATGCTCAGaaaa                                                                                                     >  1:1013549/1‑139 (MQ=255)
                                        tatGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGGTTTGCTGTAATCAATCTAGTCGCTTTTTAAGCCTGGCGGCGTCGCGCTTTTTAAACCTAACCGTTCAAAGCGCAGTTCCTCCCAATGCTCAGACAg                                                                                                     >  1:1850600/1‑140 (MQ=255)
                                          tGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCt                                                                                                   <  2:764269/140‑1 (MQ=255)
                                          tGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCt                                                                                                   <  1:1185492/140‑1 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTTACGCGAGGCGGCGGACCTGTTTTAAAACCAACCAGGTCAAAACACACTTACTCCAAAAGCTCAGAAAGCGTaa                                                                                                >  1:310546/1‑140 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTaa                                                                                                >  2:712339/1‑140 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAAAGAAAGAGGTCAAAGCCCAGTTACTCCAAATGCTAAGAAAGCTTaa                                                                                                >  2:1985895/1‑140 (MQ=255)
                                             gaAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCGTTTGACGCGAGGCGGCGTACCGGTTTTAAAAAGAAAGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTaa                                                                                                >  1:2475155/1‑140 (MQ=255)
                                                aGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAg                                                                                             <  2:1159716/140‑1 (MQ=255)
                                                     gAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgc                                                                                        <  1:503943/140‑1 (MQ=255)
                                                      aGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATTCTCAGAAAGCTTAATAGAAGgct                                                                                       >  2:903435/1‑140 (MQ=255)
                                                      aGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgct                                                                                       <  1:679429/140‑1 (MQ=255)
                                                      aGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgct                                                                                       <  1:404899/140‑1 (MQ=255)
                                                      aGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgct                                                                                       <  1:286020/140‑1 (MQ=255)
                                                      aGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgct                                                                                       <  1:1969783/140‑1 (MQ=255)
                                                       ggCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCACTCGAGTCGCTTTTGACCCCAGGCGGCGGACCTTTTTTAAAAAGAAACAGGTCAAAGCCCACTTACTCCAAATGCTCAGAAAGCGTAATAGAAGgctg                                                                                      >  2:367886/1‑140 (MQ=255)
                                                       ggCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGGGTACCTGGTTTAAAACGAACGAGGTCCAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGgctg                                                                                      >  2:1267709/1‑140 (MQ=255)
                                                          acaTCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGctgctg                                                                                   <  1:2439334/140‑1 (MQ=255)
                                                            aTCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGt                                                                                 <  2:2931173/140‑1 (MQ=255)
                                                                  aCCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCg                                                                           <  1:1629584/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCACTCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCGGCTGGTTATTCGGACCGc                                                                     <  2:1285714/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:1036166/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:1260161/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  2:1904292/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:1363423/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:1761672/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  2:2115395/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:878639/140‑1 (MQ=255)
                                                                        cTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:891034/140‑1 (MQ=255)
                                                                        cTGAATTTCCGGTTTGCTGTAATCAAGCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGATTTAAAACGAACGCGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGc                                                                     <  1:151392/140‑1 (MQ=255)
                                                                            aTTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCACTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGa                                                                 >  2:1900534/1‑140 (MQ=255)
                                                                             tttCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAg                                                                <  1:776902/140‑1 (MQ=255)
                                                                             tttCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAg                                                                <  1:191949/140‑1 (MQ=255)
                                                                                     ttGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAg                                                        <  1:2425386/140‑1 (MQ=255)
                                                                                                  tcGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTACTCCAAATGCACAGAAAGCTTAATACAAGGCTGCTGGTTATTAGGACCCCTTGAGAAACACCAATCACCAAGAATg                                           >  2:3045260/1‑140 (MQ=255)
                                                                                                       tCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCg                                      <  2:1087315/140‑1 (MQ=255)
                                                                                                        cGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGt                                     <  1:1327959/140‑1 (MQ=255)
                                                                                                        cGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGt                                     <  1:945125/140‑1 (MQ=255)
                                                                                                        cGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGt                                     <  1:1894599/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:1397285/140‑1 (MQ=255)
                                                                                                         gCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTc                                    <  2:676086/140‑1 (MQ=255)
                                                                                                            tttGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCctc                                 <  1:1394736/140‑1 (MQ=255)
                                                                                                                   cgAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGGTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAata                          <  2:1850600/140‑1 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATg                        >  2:1184679/1‑140 (MQ=255)
                                                                                                                     aGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGACCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATg                        >  2:1546098/1‑140 (MQ=255)
                                                                                                                        cggcgTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTcc                     <  2:213768/140‑1 (MQ=255)
                                                                                                                        cggcgTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTcc                     <  1:2793695/140‑1 (MQ=255)
                                                                                                                          gcgTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCt                   <  1:2093207/140‑1 (MQ=255)
                                                                                                                                  gTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCAt           >  1:2642323/1‑140 (MQ=255)
                                                                                                                                     ttAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAg                                               <  2:2502256/101‑1 (MQ=255)
                                                                                                                                     ttAAAACGAACGAGGTCAAAGCCCAGGTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGAc        <  2:1199074/140‑1 (MQ=255)
                                                                                                                                        aaaCGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAAc     <  2:2727290/140‑1 (MQ=255)
                                                                                                                                           cgaacgaGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGc  <  1:2226341/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CCAGCAAGGCAGCTGATCTGTAATATATTCCACTGTTTCATATGAGAAAGCGCGAGGCACATCGTCACCAGGCTGAATTTCCGTTTTGCTGTAATCAATCGAGTCGCTTTTGACGCGAGGCGGCGTACCTGTTTTAAAACGAACGAGGTCAAAGCCCAGTTCCTCCAAATGCTCAGAAAGCTTAATAGAAGGCTGCTGGTTATTCGGACCGCTTGAGTAAGACAGATCACCAAGAATGATTCGTCCTCTTAAATATGTCCCTGTCGTCATGACAACTGC  >  NZ_CP034943/4040290‑4040568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: