mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | PAL206_S204_L001_R1_001.good.fq | 3,113,157 | 425,782,960 | 100.0% | 136.8 bases | 140 bases | 98.7% |
| errors | PAL206_S204_L001_R2_001.good.fq | 3,113,157 | 425,782,960 | 100.0% | 136.8 bases | 140 bases | 97.9% |
| total | 6,226,314 | 851,565,920 | 100.0% | 136.8 bases | 140 bases | 98.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NZ_CP034943 | 4,045,538 | 201.8 | 14.0 | 100.0% | Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499 strain |
| total | 4,045,538 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 2915 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 57 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.004 |
| reference sequence | pr(no read start) |
|---|---|
| NZ_CP034943 | 0.77438 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 10:59:40 16 May 2023 | 11:01:28 16 May 2023 | 1 minute 48 seconds |
| Read alignment to reference genome | 11:01:28 16 May 2023 | 11:15:12 16 May 2023 | 13 minutes 44 seconds |
| Preprocessing alignments for candidate junction identification | 11:15:12 16 May 2023 | 11:16:45 16 May 2023 | 1 minute 33 seconds |
| Preliminary analysis of coverage distribution | 11:16:45 16 May 2023 | 11:20:05 16 May 2023 | 3 minutes 20 seconds |
| Identifying junction candidates | 11:20:05 16 May 2023 | 11:20:06 16 May 2023 | 1 second |
| Re-alignment to junction candidates | 11:20:06 16 May 2023 | 11:23:26 16 May 2023 | 3 minutes 20 seconds |
| Resolving best read alignments | 11:23:26 16 May 2023 | 11:25:34 16 May 2023 | 2 minutes 8 seconds |
| Creating BAM files | 11:25:34 16 May 2023 | 11:28:29 16 May 2023 | 2 minutes 55 seconds |
| Tabulating error counts | 11:28:29 16 May 2023 | 11:29:46 16 May 2023 | 1 minute 17 seconds |
| Re-calibrating base error rates | 11:29:46 16 May 2023 | 11:29:47 16 May 2023 | 1 second |
| Examining read alignment evidence | 11:29:47 16 May 2023 | 13:08:50 16 May 2023 | 1 hour 39 minutes 3 seconds |
| Polymorphism statistics | 13:08:50 16 May 2023 | 13:08:52 16 May 2023 | 2 seconds |
| Output | 13:08:52 16 May 2023 | 13:09:11 16 May 2023 | 19 seconds |
| Total | 2 hours 9 minutes 31 seconds | ||
