New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 1437935 =142 (0.650)3 (0.010) 3/248 9.8 2.2% coding (738/1821 nt) kinA sporulation kinase KinA
?NZ_CP034943 = 1437931 132 (0.650)coding (734/1821 nt) kinA sporulation kinase KinA
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCGACTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/1438080‑1437935
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCACG  <  NZ_CP034943/1437931‑1437794
                                                                                                                                                                                                                                                                                            
CCGACTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGC                                                                                                                                                  <  1:2378537/140‑1
CCGACTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATGGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTGTGGAATTTTTTCCTTGAAGAGATATCGATCAGC                                                                                                                                                  <  1:199516/140‑1
  GACTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAG                                                                                                                                                <  2:2774228/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:1139907/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:334388/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:2940975/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:2757050/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:2755538/140‑1
    CTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:2390133/140‑1
     TCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAG                                                                                                                                             <  2:894226/140‑1
     TCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAG                                                                                                                                             <  2:728143/140‑1
       ATTCATAAACACCCATTTTCCAGTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTGTGGAATTTTTTCGGGGAAGAGCTCTCGTTCAGCAGCAGCT                                                                                                                                           >  1:2115057/1‑140
                 ACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              <  1:2141128/127‑1
                 ACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCA                                                                                                                                              >  2:2141128/1‑127
                                                         AATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGA                                                                                         >  2:1220277/1‑140
                                                             TTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAG                                                                                     <  1:1220277/140‑1
                                                                                                                                          GCAGCAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTT        >  2:2683580/1‑140
                                                                                                                                             GCAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTC     >  2:426228/1‑140
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  1:307335/140‑1
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  1:913276/140‑1
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  1:101563/140‑1
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  2:2952663/140‑1
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  1:2670087/140‑1
                                                                                                                                              CAGCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCA    <  1:2352820/140‑1
                                                                                                                                                GCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCCTTCGTATAATCCTGTTTTTCACG  >  2:404757/1‑140
                                                                                                                                                GCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCACG  <  1:1638878/140‑1
                                                                                                                                                GCTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCACG  <  1:1995981/140‑1
                                                                                                                                                GCTCAGCCTGCTGGATTTTGTAGACGGTCGGGGACGCATACACTTCTACATGAACAGGTGTGCCAACAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTGTGCATTCGTATACTCCTGTTTTTCACG  <  1:3373675/140‑1
                                                                                                                                                                                                                                                                                            
CCGACTCATTCATAAACACCCATTTTCCATTATGAATCACCGCAATGGTATCGATTGAATTTTGAATCAGCAGCTGATATCGTTCACGGCTTTTTTGCAGGATGGTTTGGAATTTTTTCCTTGAAGAGATATCGATCAGCAGCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP034943/1438080‑1437935
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcagcagcTCAGCCTGCTGGTTTTTGTAGACGGTCGGGGACGCTTTCACTTCTAAATGAACAGGTGTGCCATCAAGCCTTTTCCACGTCTGTTCAATCATTCCAACTTCCATTCCTTTTTGCATTCGTATAATCCTGTTTTTCACG  <  NZ_CP034943/1437931‑1437794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.