Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0930GC25.2% 186.7 / 12.2 107E172Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (31/46);  new base C (0/26);  total (31/76)
Fisher's exact test for biased strand distribution p-value = 3.13e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 112 total aligned reads displayed.

CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAT  >  NZ_CP034943/1729954‑1730230
                                                                                                                                           |                                                                                                                                         
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                           >  2:2960263/1‑140 (MQ=255)
cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                                           >  2:3184717/1‑140 (MQ=255)
  cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                                         >  2:1078297/1‑140 (MQ=255)
     cTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAgaagaa                                                                                                                                      >  1:3234546/1‑140 (MQ=255)
       gAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAg                                                                                                                                    >  2:2224474/1‑140 (MQ=255)
          aaaTCATTTACGAAAAGTTAAAGAAATTACGAATTCACAAAAGATTCAACTCTAAAAGAGAATAAAAGAAACAGCTCTGAAAAAGAGGAAGACACTAAAAAAGAAACTAAAGAAAAAGAATATAAAAAAGAAGAAAGCGa                                                                                                                                 <  2:2289038/140‑1 (MQ=255)
               aaTTACGCAAATTTAAAGCAAGTACGAAATCCCAAAAGATTAAACGATAAAAGAGAATCAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTg                                                                                                                            <  2:2416819/140‑1 (MQ=255)
                  taccaaaaTTGAAATCAATTACTAAATCCAAAAATATTAAACGATAAAATATAATCAAAGAAAAAGATCTTAAAAAGAGGCACACACTAAAAAATAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATa                                                                                                                         <  2:1295417/135‑1 (MQ=255)
                      cacaGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAACAAGAGGCAGACAGTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGAGAAAcg                                                                                                                     >  2:3261976/4‑139 (MQ=255)
                       aaaGTGAAAGCAAGTACTAAATCCAAAAAGATTCAACGAAAAAAGACAATAAAAGCAACAGATCTGGAACAGAGTCAGACACTAAAAAACAAACGAAATAAAAAGAAGATAAAAAAGAACAACGCCACAGTGATAAACTg                                                                                                                    <  2:306920/140‑1 (MQ=255)
                         aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGga                                                                                                                  >  1:1532135/1‑140 (MQ=255)
                               aGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTaa                                                                                                            >  1:278081/1‑140 (MQ=255)
                                 aaattACTAAACCCAAAAATATAAAACTCTAAAAGACAATAAAAGCAACACCTCTTAAAAATAGGCAGAAAATAAAAAACAAACCAAACAAAAACACCATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAg                                                                                                          <  1:2365087/136‑1 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGACACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                        >  1:2228415/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAGTGAAAAAGAAGATCAAAAAGAAGAAAGCGACTGTGATAAACTGGAGATTAAAGCa                                                                                                        >  1:2532106/1‑140 (MQ=255)
                                   aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGCCAGTGATAAACTGGAGATTAAAGCa                                                                                                        >  2:1985782/1‑140 (MQ=255)
                                    ttACCAAATCCCAAAAGATTCAACGATAAAAGAGAATCAAAGCAACAGATCTGAAAAAGAGGCACACACTAAAAAAGAAACGAAAGAAAAATAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                       <  2:3288879/139‑1 (MQ=255)
                                    ttACCAAAGCCCAAACTATTAAACGCTAAAACACAATCAAAGAAACAGCTCTTAAAAAGAGGCACACAATAAAAAACAAACCAAACAAAAACACCATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                       <  2:468380/139‑1 (MQ=255)
                                    gaaCGAAATCCCAAAACATTCAACGATAAAAGAGAATCAACGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAACAAAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                       <  2:2247852/138‑1 (MQ=255)
                                    gTACTAAATCCCAAACTATTCAACGCAAAAAGAGAATCAAAGCAACAGATCTGAAAAAGAGGCAGACACTAACAAAGAAACCAAAGAAAAAGAAGCTAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                                                       <  2:3395348/140‑1 (MQ=255)
                                     tACTAAATCCAAAAATATTAAACGCTAAAAGAGAATAAAAGCAACAGATATTAAAAATAGGCAGACACTAAAAAATAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  2:2514112/140‑1 (MQ=255)
                                     tACTAAACCCAAAAATATTCAACGCAAAAAGACAAAAAAACCAACCGCTCTTAAAAAGAGGCACACACTAAAAAACAAACCAACCAAAAACAAGAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  1:2224474/140‑1 (MQ=255)
                                     tACGAGATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  2:1828774/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  2:3318276/1‑140 (MQ=255)
                                     tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      >  1:3393077/1‑140 (MQ=255)
                                     tACGAAATCACAAAATATTCAACTCTAAAAGATAATAAAAGCATCACGTCTGAAAAAGAGGCAGACACTAAAAAATAAACCAAAGAAAAAGCAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  2:1898685/140‑1 (MQ=255)
                                     tACCAAATCCAAAAAGATTCAACGATAAAACACAATCAAACAAACAGATATCAAAAATAGGCAGACAATAAAAAACAAACCAAACAAAAACAACATAAAAACCACCAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                                      <  1:2289783/140‑1 (MQ=255)
                                        caaaTCAAAAAAGATTAAACGATAAAATAGAATAAAAGAAAAAGAAATTAAAAAGAGCCATACACTAAAAAAGAAACGAAAGAAAAAGAATATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                                   <  2:2718054/139‑1 (MQ=255)
                                         aaaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAATAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGc                                                                                                  <  2:1532135/140‑1 (MQ=255)
                                          aaTCACAAAATATACAACCCTAACACACAAACAACCAACCCGCACTCAAAAAGAGCCAAAAAATAAAAAACAAACAAAACAAAACGACTATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACGGGCa                                                                                                 <  1:1128421/140‑1 (MQ=255)
                                              ccAAAAGATTCAACGCTAAAAGACAATCAAAGCAACAGCTATTAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                             <  2:2504112/140‑1 (MQ=255)
                                              ccAAAAGATTCAACGATAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACTAAAGAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                             <  2:2504084/140‑1 (MQ=255)
                                               caaacttttCAACGCCAAAATCTAATAAAACCAACCCCTTATAAAAACACGCACACAAAAAAAAACAAACCAAAAAAAAACAACAAAAAAAACACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                            <  1:548018/133‑1 (MQ=255)
                                               cAAAAGATTCAACTCTAAAAGAGATACAAAGCAACAGCTCTGAAAAAGATCCAGAAACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAACACGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                            <  2:2125673/140‑1 (MQ=255)
                                               cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                            >  2:864452/1‑140 (MQ=255)
                                                  aataTTCAACTATAAACTATAATAAAATCAACATATATCAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAACTACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                         <  2:1390159/137‑1 (MQ=255)
                                                     aTTCAACGCTAAAAGATAATCAAACCAACAGCTCTTAAAAATAGCCACAAAATAAAAAACAAACTAAAGAAAAACACTCTAAAAAAGACCAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCa                                                                                      <  2:1268303/140‑1 (MQ=255)
                                                     aTTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCCACGAAAAAGAAGAAAAAAAACAAGAAAGCGACCGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCa                                                                                      <  2:799757/140‑1 (MQ=255)
                                                     aTTCAACGCAAAACGATAAGAAAACAAACAGATATGAAAAACAGGCAGACACTAAAAAACAAACGAAACAAAAACAAGATAAAAAATAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCa                                                                                      <  2:1908692/140‑1 (MQ=255)
                                                       tCAACGCAAAAAGAGAATCAAACCAACAGCTCTGAAAAAGACGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAACTAAAACACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                                    <  2:782479/140‑1 (MQ=255)
                                                        aaactatAAAAGATAATCAAAGCAACAGCTCTTAAAAATAGGCAGACAATAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTa                                                                                   <  2:3139835/134‑1 (MQ=255)
                                                          acccTAAAAGATAAACAAAGAAACAGCTATTAAAAATACGAACACAAAAAAAAAAAAACCAAATAAAAACAACAAAAAAAACAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  1:164073/137‑1 (MQ=255)
                                                          accaaaaaaTATAAACAAACAAACAGCTCTTAAAAAGACCCACACACTAAAAAAAAAACCACAAAAAAACAAAAAAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  1:1355199/135‑1 (MQ=255)
                                                          aCGCTAAAATAGAATCAAAGCACCAGCTCTCAAACTGAGCCAGACAATAAAAATGAAACGCACCCAAAAGAATACAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:252032/140‑1 (MQ=255)
                                                          aCGCTAAAAGATAATAAAAGCAACAGATCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAACCAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:736344/140‑1 (MQ=255)
                                                          aCGCTAAAAGACAATCAAACCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAATAAACCAAACAAAAACAACACAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGTAGGGTCATTAAc                                                                                 <  2:278081/140‑1 (MQ=255)
                                                          aCGCTAAAACACAATAAAACCAACAGATCTGAAAAAGAGGCACACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:169219/140‑1 (MQ=255)
                                                          aCGATAAAAGATAATAAAAGCAACAGATCTGAAAAAGTGGCAGACACTAAAAAAGAAACGAAAGAACAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:1090709/140‑1 (MQ=255)
                                                          aCGATAAAACACAATAAAACAAACAGCTCTGAAAAAGAGCAAGACACTAAAAAACAAACGAAACAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                                 <  2:2748421/140‑1 (MQ=255)
                                                                aaaGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTa                                                                           >  1:3098628/1‑140 (MQ=255)
                                                                  agagAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                         <  2:3179069/140‑1 (MQ=255)
                                                                    tcaaTAAAACCAAACGCTCATAAAAATAGGCAGACACTAAAAAACAAACCAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:513282/138‑1 (MQ=255)
                                                                    ctaaTCAAAGCAAAAGCTCTTAAAAAGAGGCACACACTAAAAAAGAAACCAACCAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:2699722/138‑1 (MQ=255)
                                                                    ataaaaaaaCCAACCGCTCTTAAAAAGAGGAACACACTAAAAAACAAACCAAACAAAAACAACAAAAAACACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  1:1513907/134‑1 (MQ=255)
                                                                    agaATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:3234546/140‑1 (MQ=255)
                                                                    agaATAAAAGCAACAGCTCTGAAAAAGAGCCAGACACTAAAAACTAAACGAAAGAAAAAGAATATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:1528664/140‑1 (MQ=255)
                                                                    agaATAAAACCAACCGCTCTGAAAAAGAGCCAGACACTAAAAAACAAACGAAAGAAAAAGACGATAAAACACACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:1378879/140‑1 (MQ=255)
                                                                    agaATAAAACCAACAGCTCTCAAAAACAGCCACACACTAAAAAATAAACCAAACAAAAACACTTTAAAAAAGAAGAAAGCGACATTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  2:1018312/140‑1 (MQ=255)
                                                                    agaAACAACGAAACAGCTCTCAAAAACAGCCACACACAAAAAAACAAACGAAAGCAAAATAAGATAAAAAAGACGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                       <  1:789655/140‑1 (MQ=255)
                                                                      aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                                     >  1:2868740/1‑140 (MQ=255)
                                                                        tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                   >  1:1626489/1‑140 (MQ=255)
                                                                        tCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtgt                                                                   >  2:425401/1‑140 (MQ=255)
                                                                         cAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACCGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:3393077/140‑1 (MQ=255)
                                                                         cAAAGCAACACCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAAGAAAAAGACGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:3069069/140‑1 (MQ=255)
                                                                         cAAAGAAACAGCTCTCAAAAAGAGCCAGACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAAACACCACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:2326961/140‑1 (MQ=255)
                                                                         aaaaGCAAAAGATATGAAAAATAGGCAGAAACTAAAAAAGAAACGAAACAAAAAGAACATAAAAAAGAAGCAAGCGACCGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:2952571/139‑1 (MQ=255)
                                                                         aaaaGAAACATCTCTGAAAACGAGGCAGAAACTAAAAAAGAAACGACACAAAAACACGATAAAAAACCAGAAAGCGACAGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTc                                                                  <  2:3069127/139‑1 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  2:389509/1‑140 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  2:2905384/1‑140 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  1:3062400/1‑140 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  1:3374155/1‑140 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  2:1548824/1‑140 (MQ=255)
                                                                                         aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                                  >  1:534103/1‑140 (MQ=255)
                                                                                             atagccAGACAAAAAAAAAAAACCCAAAAAAAAACAAGAAAAAAAAAAAAAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                              <  1:2981384/135‑1 (MQ=255)
                                                                                              gagGCAGACACTAAAAAATAAACCAAATAAAACGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             <  2:496751/140‑1 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  2:2886254/1‑140 (MQ=255)
                                                                                              gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:1862589/1‑140 (MQ=255)
                                                                                                   aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                                        >  2:2896003/1‑140 (MQ=255)
                                                                                                      cacTAAAAAACAAACCAAACAAAAATAATATACAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  1:1945970/140‑1 (MQ=255)
                                                                                                      aacTAAAAAATAAACCAAACAAAAATAATATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  1:1049158/139‑1 (MQ=255)
                                                                                                      aaaaaaaaaaCAAACCAAACAAAAAGAATAAAAAACAGAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                                     <  1:1806778/136‑1 (MQ=255)
                                                                                                           aaaaaGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCa                                <  2:3098628/140‑1 (MQ=255)
                                                                                                                taaaCGAATCAAAAACAAGATAAAAATGAAGAACGCGAGAGTGATAAACTGGAGATTAAAGCAACGGGCACTGAAGGGTCATTAACGAATTATGAAGTGTCCGGCGCTGATAAATTCGATCTCGAACTGAAGGCATCAGa                           <  2:300653/139‑1 (MQ=255)
                                                                                                                taaaCGAAATAAAAAGAAGATAAAAAAGAAGACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:1071225/139‑1 (MQ=255)
                                                                                                                gAAACTAAAGAAAAACAAGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:3103940/140‑1 (MQ=255)
                                                                                                                gAAACGAAAGAACATCCATATACAACTGCAGAAAGCGTCAGTGATACACTGGATATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:440685/140‑1 (MQ=255)
                                                                                                                gAAACGAAAGAAAAAGAAGATAAAACACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:3281119/140‑1 (MQ=255)
                                                                                                                gAAACCAAAGAAAACAAACATAAAACACAACACAGCGACACTGATAAACTGTAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:912681/140‑1 (MQ=255)
                                                                                                                caaaCGAAATAAAAAGAAGAAAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                           <  2:2868740/139‑1 (MQ=255)
                                                                                                                 aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc                          >  1:1330763/1‑140 (MQ=255)
                                                                                                                 aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc                          >  2:558979/1‑140 (MQ=255)
                                                                                                                 aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGAAACAGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTAGAGCTCGAACTGAACGCATCAGAc                          >  2:2638055/1‑140 (MQ=255)
                                                                                                                   accaaataaaaagacGCAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGCTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                        <  1:2541458/137‑1 (MQ=255)
                                                                                                                   accaaacaaaaagaaTATAAAAAACAACCACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                        <  1:928619/137‑1 (MQ=255)
                                                                                                                   aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAATTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                        >  2:548773/1‑140 (MQ=255)
                                                                                                                           aaaccaagaTAAAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa                <  2:2097826/135‑1 (MQ=255)
                                                                                                                           aaaacaaGATAAAAAAGAATAACCCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGa                <  2:394714/140‑1 (MQ=255)
                                                                                                                              agaagaTAAAAAAGAAGAAAGCGACAGTGATAACGTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTc             <  2:3195055/140‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                                             >  1:1619055/1‑103 (MQ=255)
                                                                                                                                         cagaacaAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAt  <  1:2033458/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGTACGCGAT  >  NZ_CP034943/1729954‑1730230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑