Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,408,7270CA26.8% 227.7 / 23.1 138A327S (GCG→TCG) spoIIIAEstage III sporulation protein AE
Reads supporting (aligned to +/- strand):  ref base C (40/61);  new base A (0/37);  total (40/98)
Fisher's exact test for biased strand distribution p-value = 3.23e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 142 total aligned reads displayed.

GCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAGCCG  >  NZ_CP034943/2408588‑2408864
                                                                                                                                           |                                                                                                                                         
gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                           >  2:93931/1‑140 (MQ=255)
gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                           >  1:1449950/1‑140 (MQ=255)
gCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGc                                                                                                                                           >  2:2874237/1‑140 (MQ=255)
    cAAAAATATAACTGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:712125/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       >  1:498649/1‑140 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:1038420/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:2290483/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       >  2:470434/1‑140 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       >  2:3253781/1‑140 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:214716/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:2969264/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:2806337/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:2453609/140‑1 (MQ=255)
    cAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       >  2:1620100/1‑140 (MQ=255)
    cAAAAATATAAATGACGCTTTAGCTGATGACGTCCAGGCAGGTAATTACGGGCCCGCCTCCAAGCGGATGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGCGGGAAAGCACTTTGATTGCCGGAACAGCCGctaga                                                                                                                                       >  2:2492483/1‑136 (MQ=255)
    cAAAAATAGAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATa                                                                                                                                       <  1:2716003/140‑1 (MQ=255)
      aaaaTATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACa                                                                                                                                     <  2:1569114/140‑1 (MQ=255)
               aTGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAAt                                                                                                                            >  2:104089/1‑140 (MQ=255)
                  ccGCTTTTGCTGATGACGTCGAGGCAGTTAATGACGGGCCCGCCTCCACGCGGCTTAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTTATTGCCGTAAAAGCCGCAATACAGATCAAAATCGc                                                                                                                         <  2:599990/139‑1 (MQ=255)
                              atgaCGTCGTGCCAGTTACTGCCGCGCCCGCCTCCAAGGGGCTCAAGAATCGGGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGCAAAAGCCGAAATACAGAACAAACTAGCGACTCCGAGAAt                                                                                                             <  1:1516675/140‑1 (MQ=255)
                              atgaCGTCGAGGCAGGTAATGAGGGGCCCGGCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGTTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCGGTAAACGCCGCAATACACATAAAAATCGCGACTCCGAGAAt                                                                                                             <  1:1002398/140‑1 (MQ=255)
                              atgaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAAt                                                                                                             >  1:2125171/1‑140 (MQ=255)
                                gaCGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATcc                                                                                                           >  2:1840130/1‑140 (MQ=255)
                                        ggCAGTTAATGCCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAACTAAACGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                   <  2:1851005/140‑1 (MQ=255)
                                        ggCAGTTAATCCGGGGCCCGCCACCCCGCGGCTTAAGAATCGCGGCGGCCAGTTTATAAATAAAAGCGAGGTAACGCACTTTGTTTGCCGTAAAAGCCGAAATACATATAAAAATCGCGACTCCGAGAATCCCTACTGTa                                                                                                   <  1:1002875/140‑1 (MQ=255)
                                        ggCAGGTAATGCCGGGCCCGCCTCCACGCGGCTGAAGAACCGCGGCTGCCAGCTTATAAATAAAACCGAGGGAAAGCACTTGGATGCCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                   <  2:1050524/140‑1 (MQ=255)
                                        ggCAGGTAATCCCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTGGATGGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTa                                                                                                   <  2:3213052/140‑1 (MQ=255)
                                            ggTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTAtttt                                                                                               >  2:184743/1‑140 (MQ=255)
                                             gTAATGACGGGCCCGCCTCCACGCGGCTCAAGAATCGCGGCTGCCACATTATAAATAAAAGCGAGGGAACCCACTTTGTTTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGACAATCCCTACTGTAttttt                                                                                              <  1:2067131/140‑1 (MQ=255)
                                              tattgccGGGCCCGCCTCCACGCGGCTGCAGAATCGCGGCTGCCAGCTTATAAATAAACGCTCGGGAAAGCAGTTTGTTTGCCGGAAACGCCGCAAGACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                             <  2:2950988/134‑1 (MQ=255)
                                              tAATGACGGGCCCGCCCCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAACGCACTTTGATTGCCGGAAAAGCCGCAATACAGACCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTc                                                                                             <  2:821770/140‑1 (MQ=255)
                                                aTGCCGGGCCCGCCACCACGGGGCTGAAGAAGCGGGGCTGCCAGTTTATAAATAAACGCGAGGTAAACAATTTTTTTTGCCGCAAAAGCCCAAAAACAGAAAAAAAACGCGACTCCGAGAATCCCTACTGTATTTTTcag                                                                                           <  1:1281102/140‑1 (MQ=255)
                                                   ccGGCCCCGCCTCCAGGCGGCTTAACAATCGCGGCTGCCATCTTATAAAAAAACGCGCGGTAAACCAGTTTCTTTGCCGCAAAACCCGAAATACCGAAAAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                        <  1:2633950/139‑1 (MQ=255)
                                                   aCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAACGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcagcag                                                                                        <  2:2486111/140‑1 (MQ=255)
                                                   aCGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTcagcat                                                                                        >  2:2384632/1‑139 (MQ=255)
                                                      ggCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTaa                                                                                     >  2:34458/1‑140 (MQ=255)
                                                      ggCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTaa                                                                                     >  2:2791932/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGACTCCCTCCTGTATTTTTAAGCAGTAAg                                                                                    >  1:1890483/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGACTCCCGACTGTATTTGTCAGCAGTAAg                                                                                    >  1:170827/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTCCTGTATTTTTCAGCTGTAAg                                                                                    >  1:1890090/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                    >  2:445102/1‑140 (MQ=255)
                                                       gCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAg                                                                                    >  1:667895/1‑140 (MQ=255)
                                                        cccGCCTCCACGCGGCTTAACAAGCGCGGCTGCCAGCTTATAAATAAACGCTAGGTAAAGAACTTTTATTGCCGTAAAAGCCGAAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                   <  1:2847827/140‑1 (MQ=255)
                                                        cccGCCGCCACGCGGCTTAAGAAGCGCGGCTGCCAGATTATAAATAAACGCGAGGTAAAGAACTTTGATTGCCGCAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAgg                                                                                   <  1:977512/140‑1 (MQ=255)
                                                          cGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGTAACGCTCTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGaa                                                                                 <  2:2536963/140‑1 (MQ=255)
                                                          cGCCCCCACGCGGCTCAACAAGCGCGGCTCCCACATTAAAAAAAACCGCGAGGCAAACCAGTTTGATGGCCGCAAAACCCTAAATACACATCAAAACCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGaa                                                                                 <  1:341720/140‑1 (MQ=255)
                                                             cTCCAAGCGGCTTAAGAATCGCGGCTGCAAGCTTATAAATAAAAGCGCGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAgcg                                                                              <  2:3016790/140‑1 (MQ=255)
                                                               cccaGCGGCTCAAGACTCGCGGCTGCCAGATTATAAATAAACGCGCGGGAACCCACTTTGTTTGCCGCAAACGCCGCAATACACAACAAAAACGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                            <  1:1147485/137‑1 (MQ=255)
                                                               ccACGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGCAACGCACTTTGATTGCCGTAAAAGCCGAAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                            <  2:2134562/140‑1 (MQ=255)
                                                               ccAAGCGGCTGAAGTATCGCGGCTGCCATCTTATAAAAAAACGCTCGGTAAAGAACTTTTTTTGCCGTAAAAGCCGAAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                            <  1:2628312/140‑1 (MQ=255)
                                                               ccAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                            <  2:2393363/140‑1 (MQ=255)
                                                               ccAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCt                                                                            <  2:1031513/140‑1 (MQ=255)
                                                                  cgCGGCTGAAGAAGCGCGGCTGCCAGCTTATAAATAAACGCGAGGGAAAGAACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGAt                                                                         <  1:359711/139‑1 (MQ=255)
                                                                   gCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATc                                                                        <  2:2489505/140‑1 (MQ=255)
                                                                   gCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATc                                                                        <  2:2143833/140‑1 (MQ=255)
                                                                   gCGGCTGAAGAATCGCGGCTGCAAGCTTATAAATAAACGCTAGGGAACGCACTTTGATTCCCGCAAAAGCCGCAATACACATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATc                                                                        <  2:1357940/140‑1 (MQ=255)
                                                                     ggCTGAATAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGCAAACCACTTTGATTGCCGTAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                      <  2:3075490/140‑1 (MQ=255)
                                                                     ggCTCAACACTCGCGGCTGCAAGCTAATAAATAAAAGCGCGGTAAAGCACTTTTATTGCCGCAAAAGCCGAAATACACAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                      <  1:1831720/140‑1 (MQ=255)
                                                                     ggCCGACGAAGCGCGGGCGCCAGATTAACAAAACCAGCGCGGCAACGCACTTTGTTTGCCGCAAACGCCGCAATACCGATCAAATTCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcac                                                                      <  1:2413596/140‑1 (MQ=255)
                                                                      gCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATcaca                                                                     >  1:785555/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                    >  2:1434593/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                    >  1:345600/1‑140 (MQ=255)
                                                                       cTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAg                                                                    >  2:401979/1‑140 (MQ=255)
                                                                         gaagaaTCGCGGCTGCCAGATTATAAATAAACGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTa                                                                  <  1:2368665/140‑1 (MQ=255)
                                                                          aagaaTCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTAt                                                                 >  1:391750/1‑140 (MQ=255)
                                                                               tCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTc                                                            >  1:3065076/1‑140 (MQ=255)
                                                                                cgcgGCTGCCAGCTTATAAATAAACGCGTGGGAAAGCACTTTGATTGCCGGAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                           <  1:1714083/140‑1 (MQ=255)
                                                                                cgcgGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                           <  2:2943375/140‑1 (MQ=255)
                                                                                cccgGCAGCCAGCTGAGAAACACAAGCCAGGGAAAGCACTGTGATTGCCGGAAAAGCCGCAAGACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                           <  2:1992431/138‑1 (MQ=255)
                                                                                   ggCTGCCAGTTTATAAATAAACGCGTGGCAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGt                                                        <  1:3219814/140‑1 (MQ=255)
                                                                                   ggCTGCCAGCTTATAAATAAACGCGAGGCAACGCACTTTGTTTGCCGTAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGt                                                        <  1:3219677/140‑1 (MQ=255)
                                                                                        ccAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTa                                                   <  2:2235009/140‑1 (MQ=255)
                                                                                              tataAATAAACGCGCGGTAAAGCACTTTGATTGCCGTAAACGCCGAAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACAtt                                             <  1:2902750/140‑1 (MQ=255)
                                                                                                   ataataGCTAGGCAAAGCACTTTTATTGCCTCAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  2:3181233/140‑1 (MQ=255)
                                                                                                   ataaaCGCGCGGGAAAGCACTGTGATTGCCTGAAACGCCGAAAGACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  1:3106910/140‑1 (MQ=255)
                                                                                                   ataaaAGCGAGGTAAACCACTTTGATTGCCTTAAAAGCCGAAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  2:559801/140‑1 (MQ=255)
                                                                                                   ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  2:3328964/140‑1 (MQ=255)
                                                                                                   ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  1:1434593/140‑1 (MQ=255)
                                                                                                   ataaaAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATAAATAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGcc                                        <  2:2125171/140‑1 (MQ=255)
                                                                                                     aaaaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCaa                                      >  2:1060509/1‑140 (MQ=255)
                                                                                                      aaaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACAtt                                             <  2:2843423/132‑1 (MQ=255)
                                                                                                       aaGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGt                                    >  1:3150307/1‑140 (MQ=255)
                                                                                                        cgCGTGGTAACGCACTTTGTTGGCCGTAAAAGCCGAAATACAGATCAAAACAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                   <  1:1196339/139‑1 (MQ=255)
                                                                                                        aGCGTGGGAACGCAGTTTTAGTGCCGAAAACGCCGCTATACAGTTCAAACTAGCGACTCCGAGCATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                   <  1:2454437/140‑1 (MQ=255)
                                                                                                        aGCGAGGTAAAGCACTTTGATTGCCGCAAAAGCCGAAATACCGAAAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                   <  2:43068/140‑1 (MQ=255)
                                                                                                        aGCGAGGTAAAGAACTTTGATTGCCGCAAACGCCGAAATAAATATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                   <  1:2955875/140‑1 (MQ=255)
                                                                                                        aGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTa                                   <  1:1620100/140‑1 (MQ=255)
                                                                                                                aaaGCTCTTTGTTTGCGGGAAAAGCCGAAATACAGATCAAAATAGAGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATa                           <  1:2078591/140‑1 (MQ=255)
                                                                                                                aaaGCACTTTTATTGCCGTAAACGCCGCAATACAGATCAAAATCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATa                           <  1:2699873/140‑1 (MQ=255)
                                                                                                                  aGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTcg                                                           >  1:2849125/1‑106 (MQ=255)
                                                                                                                       tttGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                    <  2:1756970/140‑1 (MQ=255)
                                                                                                                       tttGATTGCCGGAAAAGCCGCAAAACAGAAAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                    <  1:3014803/140‑1 (MQ=255)
                                                                                                                       tttGATGCCCGCAAAACCCGAAATACAGAACAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                    <  1:3014488/140‑1 (MQ=255)
                                                                                                                       attGATTGCCGGAAAAGCCGCAATACAGATAAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTc                    <  2:1887740/139‑1 (MQ=255)
                                                                                                                               ccGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtata            >  1:428133/1‑140 (MQ=255)
                                                                                                                               ccGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTtata            >  1:983574/1‑140 (MQ=255)
                                                                                                                                      aGCCGCAATACATATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAg     <  2:3027730/140‑1 (MQ=255)
                                                                                                                                      aGCCGAAATACATATCAAACTCGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAg     <  2:2196510/140‑1 (MQ=255)
                                                                                                                                        ccGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAGcc   <  2:2485806/140‑1 (MQ=255)
                                                                                                                                         cGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAGCCg  >  2:1045297/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                         
GCTGCAAAAATATAAATGACGCTTTTGCTGATGACGTCGAGGCAGGTAATGACGGGCCCGCCTCCAAGCGGCTGAAGAATCGCGGCTGCCAGCTTATAAATAAAAGCGAGGGAAAGCACTTTGATTGCCGGAAAAGCCGCAATACAGATCAAAATAGCGACTCCGAGAATCCCTACTGTATTTTTCAGCAGTAAGGAAGCGCTGATCACAGTATCTGTCGCGTCTGTAAACATTCGGCCAAGTACAGGAATAAAGTTTCCGGTTATAAATTTAGCCG  >  NZ_CP034943/2408588‑2408864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑