BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP034943	132,185	G→A	D560N (GAC→AAC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,185	0	G	A	99.7%	1369.5 / NA	353	D560N (GAC→AAC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (214/137); minor base C (1/0); total (216/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

AAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGA  >  NZ_CP034943/132057‑132320

   gcgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCaaaa                                                                                                                           <  1:1455839/140‑1 (MQ=255)

   gcgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCaaaa                                                                                                                           <  1:2223971/140‑1 (MQ=255)

      cttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACtt                                                                                                                        >  1:709959/1‑140 (MQ=255)

       ttgcttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACttt                                                                                                                       >  2:2895348/1‑140 (MQ=255)

           tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:2655701/140‑1 (MQ=255)

               aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAGATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGt                                                                                                               <  2:2519885/140‑1 (MQ=255)

               aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGt                                                                                                               <  2:2731099/140‑1 (MQ=255)

                 tttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTc                                                                                                             <  1:1828371/140‑1 (MQ=255)

                 tttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACAT‑CCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                            <  2:1451843/140‑1 (MQ=255)

                  ttgtaAATGCTCTTGTCGGTGGGGTAGTTCCAGGTGAATACATCCCATCTGTTCAAGCAGGTCTGGATGATGCACGTGAAAAGGGTGTACTGGCAGGCTTCCCGCTTATCAACAGTAAAGCAAAACTTTTCGATGGTTCt                                                                                                            <  1:398540/140‑1 (MQ=255)

                       aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                       <  2:1376864/140‑1 (MQ=255)

                                            gTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                                                  <  2:3016983/140‑1 (MQ=255)

                                             ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                 <  1:3159668/140‑1 (MQ=255)

                                             ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                 <  1:3207054/140‑1 (MQ=255)

                                               ccACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGc                                                                               >  1:778324/1‑140 (MQ=255)

                                                  cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:895144/101‑1 (MQ=255)

                                                  cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   >  1:895144/1‑101 (MQ=255)

                                                  cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  2:2275553/1‑140 (MQ=255)

                                                  cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  1:2707312/1‑140 (MQ=255)

                                                  cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  2:1905320/1‑140 (MQ=255)

                                                    tGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACAATGATGTTGACTCAAACGAAATGGCAtata                                                                          >  1:2046893/1‑140 (MQ=255)

                                                      aaTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaa                                                                        <  1:3236624/140‑1 (MQ=255)

                                                       aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAg                                                                       >  1:1295834/1‑140 (MQ=255)

                                                       aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAg                                                                       >  2:1000419/1‑140 (MQ=255)

                                                           aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                   >  2:41063/1‑140 (MQ=255)

                                                            tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  <  2:305293/140‑1 (MQ=255)

                                                             cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 <  1:135696/140‑1 (MQ=255)

                                                                 gCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTCAAGTGgctgctgc                                                             >  1:2551163/1‑138 (MQ=255)

                                                                 gCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTc                                                             >  2:2920615/1‑140 (MQ=255)

                                                                   tGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTa                                                           <  2:1193421/140‑1 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                       <  2:2236700/94‑1 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  <  1:437821/131‑1 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  <  1:650943/131‑1 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  >  2:650943/1‑131 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTCTg                                                         >  1:1914731/1‑140 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  1:1123128/1‑140 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  1:2895603/1‑140 (MQ=255)

                                                                     ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  2:1683322/1‑140 (MQ=255)

                                                                       cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                                                  <  2:122621/113‑1 (MQ=255)

                                                                        aaGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                      <  1:1000419/140‑1 (MQ=255)

                                                                             ggTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaa                                                 >  2:2404432/1‑140 (MQ=255)

                                                                               tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:2255239/72‑1 (MQ=255)

                                                                               tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   >  1:2255239/1‑72 (MQ=255)

                                                                               tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                               >  1:759923/1‑140 (MQ=255)

                                                                               tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                               >  1:1966242/1‑140 (MQ=255)

                                                                                  ggAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                            >  1:1237359/1‑140 (MQ=255)

                                                                                    aaGATGCACTTGAAACTGCTGGACTGGCAGGCTTCCCGCTTAACAACCTGAAAGCACAACTTTTCGATGGTTATTACCATGATGTTGACTCAACCGAAATGGCATTTAACGTTGCTGCTTCTATGGCCTTGAAAAATGCa                                          <  2:340170/140‑1 (MQ=255)

                                                                                    aaGATGCACTTGAAAATGGTGTACAGGCAGGCTTCCCGCTTATCACCATTAAAGCAAAACTTTTCGATGGTTCTTAACATGATGTTGACTCAAACGAAATGGAATTTAAAGGTGCTGCTTCTATGGCATTGAAAAATGCa                                          <  1:815336/140‑1 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGGTGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1073860/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                       <  1:2471839/75‑1 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  >  1:209658/1‑112 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  <  2:209658/112‑1 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                            >  1:43130/1‑134 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                            <  2:43130/134‑1 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:765928/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:3315561/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:2513013/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:3179000/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:1532337/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:393018/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:2786060/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:1858771/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:2123279/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:1798515/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:59190/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1726559/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:3008225/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:156737/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:1083161/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:693762/1‑140 (MQ=255)

                                                                                        tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAAAATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:819951/1‑140 (MQ=255)

                                                                                         gCACTTGAAAATGGTGTACTGGCAGGCGTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 <  2:1356513/112‑1 (MQ=255)

                                                                                          cACTTGAAAATTCTGTACAGGCAGGCTACCCTCTTATCAACATTAAAGCAAAACTTTGCGAGGGTTCTTACCATGATGTTGCCTCAAACGAAATGGCAGTTAAACTTTCAGCTTCGAGCCCATTGAAAAATGCAGTAAga                                    >  2:1953308/1‑139 (MQ=255)

                                                                                          cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                    <  1:1612795/140‑1 (MQ=255)

                                                                                          cACGTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTACTGACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGACAAATGCAGTCAGc                                    <  2:3139776/140‑1 (MQ=255)

                                                                                           aCTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCa                                   >  2:527166/1‑140 (MQ=255)

                                                                                               gAAAATTGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  1:1471610/140‑1 (MQ=255)

                                                                                               gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  1:2394067/140‑1 (MQ=255)

                                                                                               gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  1:2761256/140‑1 (MQ=255)

                                                                                                  aagggTGGACTGGCAGGCGACCCGCTGATCAACATTAAAGCAAAACTTTTCGATGCTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTCAAGGTGCTGCTTCTATGGCATAGAAAAATGCAGTCAGCAAATGTaa                            <  1:3325340/137‑1 (MQ=255)

                                                                                                  aaTGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTaa                            <  1:1037519/140‑1 (MQ=255)

                                                                                                       tgtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                       >  1:541848/1‑140 (MQ=255)

                                                                                                       tgtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                       >  1:3125987/1‑140 (MQ=255)

                                                                                                        gtACTTGCATGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTAGATGGTTCTTACCATGATGTTGACTCAAACGAAATGTCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:2585745/140‑1 (MQ=255)

                                                                                                        gtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:2988284/140‑1 (MQ=255)

                                                                                                        gtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:1488365/140‑1 (MQ=255)

                                                                                                         tACTGGCAGTCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                     <  2:362241/140‑1 (MQ=255)

                                                                                                              gCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                            <  1:2955208/112‑1 (MQ=255)

                                                                                                              gCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTtcttct                >  1:2625529/1‑140 (MQ=255)

                                                                                                                 ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa             >  2:2374816/1‑140 (MQ=255)

                                                                                                                 ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTAAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa             >  2:3255781/1‑140 (MQ=255)

                                                                                                                   cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           <  1:2445715/140‑1 (MQ=255)

                                                                                                                   cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           >  1:2194497/1‑140 (MQ=255)

                                                                                                                      cccGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCaa        >  2:1414415/1‑140 (MQ=255)

                                                                                                                       ccGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAAt       <  2:200651/140‑1 (MQ=255)

                                                                                                                       ccGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAAt       <  2:1838343/140‑1 (MQ=255)

                                                                                                                       ccGCTTATAAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAAt       <  2:443795/140‑1 (MQ=255)

                                                                                                                          cTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt    <  1:2308408/140‑1 (MQ=255)

                                                                                                                          cTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt    <  1:2425769/140‑1 (MQ=255)

                                                                                                                          cTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt    <  1:1683322/140‑1 (MQ=255)

AAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGA  >  NZ_CP034943/132057‑132320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`