Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,0800GT25.1% 421.8 / 25.9 212R31L (CGT→CTT) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base G (80/78);  new base T (0/53);  total (80/132)
Fisher's exact test for biased strand distribution p-value = 6.85e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

AGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTT  >  NZ_CP034943/548942‑549218
                                                                                                                                          |                                                                                                                                          
  ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACTCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCACAGAATACATGCATCAGGGGCGTAt                                                                                                                                         >  1:2221350/1‑140 (MQ=255)
  ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                                         >  1:2711296/1‑140 (MQ=255)
  ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                                         >  1:2837935/1‑140 (MQ=255)
  ccAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTAt                                                                                                                                         >  2:2353787/1‑140 (MQ=255)
     gTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCa                                                                                                                                      <  1:2353787/140‑1 (MQ=255)
       tttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaa                                                                                                                                    <  1:2024144/140‑1 (MQ=255)
       tttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTACGCTTTATCATCATCTTTATCGCTATTTCAAAGAAAACATGCATCAGGCGCGTATTCaaa                                                                                                                                    <  2:318494/140‑1 (MQ=255)
        ttCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                                                   <  1:2133422/140‑1 (MQ=255)
        ttCACAAAAAAATGCATACCAGTTAGGAGAAAAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                                                   <  1:801691/140‑1 (MQ=255)
        ttCACAAAAAAATGCAGACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCaaaa                                                                                                                                   <  1:463647/140‑1 (MQ=255)
          cacaAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAg                                                                                                                                 >  1:1225459/1‑140 (MQ=255)
          cacaAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAg                                                                                                                                 >  1:92584/1‑140 (MQ=255)
            caAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGa                                                                                                                               >  2:2751349/1‑140 (MQ=255)
               aaaaaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATg                                                                                                                            <  1:955626/140‑1 (MQ=255)
                aaaaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATATAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCAGCAGGGGCGTATTCAAATAGGAATGa                                                                                                                           >  1:1453247/1‑140 (MQ=255)
                  aaTGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAg                                                                                                                         <  1:1795790/140‑1 (MQ=255)
                     gCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                      <  2:1486867/140‑1 (MQ=255)
                     gCATAACAGTTAGGAGAACAACCCACATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTc                                                                                                                      <  2:2175293/140‑1 (MQ=255)
                       aTACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTccc                                                                                                                    <  1:2585347/140‑1 (MQ=255)
                               tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                            <  2:1224534/140‑1 (MQ=255)
                               tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                            <  1:1635455/140‑1 (MQ=255)
                               tAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCaaaa                                                                                                            <  1:300605/140‑1 (MQ=255)
                                aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                                           >  1:1493619/1‑140 (MQ=255)
                                aGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAg                                                                                                           >  1:2551710/1‑140 (MQ=255)
                                 ggAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGa                                                                                                          <  2:3155888/140‑1 (MQ=255)
                                   agaACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTCTCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACt                                                                                                        <  2:1333312/140‑1 (MQ=255)
                                     aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                      >  2:1365123/1‑140 (MQ=255)
                                     aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                      >  2:1033623/1‑140 (MQ=255)
                                     aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                      >  2:2494953/1‑140 (MQ=255)
                                     aacaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTcc                                                                                                      >  1:2436407/1‑140 (MQ=255)
                                       caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                    >  1:1656691/1‑140 (MQ=255)
                                       caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTGTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                    >  1:3041471/1‑140 (MQ=255)
                                        aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAATACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   >  1:1700251/1‑140 (MQ=255)
                                        aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATTAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   >  2:2627248/1‑140 (MQ=255)
                                        aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   >  1:1352702/1‑140 (MQ=255)
                                        aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTGTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   >  2:3141677/1‑140 (MQ=255)
                                           ccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATAGTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGa                                                                                                <  1:2769642/140‑1 (MQ=255)
                                            cAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAg                                                                                               >  2:707710/1‑140 (MQ=255)
                                                    tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   <  1:2447631/128‑1 (MQ=255)
                                                    tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                   >  2:2447631/1‑128 (MQ=255)
                                                    tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                       >  1:2896686/1‑140 (MQ=255)
                                                     atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                      >  1:877316/1‑140 (MQ=255)
                                                     atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                      >  2:2205053/1‑140 (MQ=255)
                                                     atCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTc                                                                                      >  1:2043720/1‑140 (MQ=255)
                                                       cACGCCTTTTGTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                    <  2:3331373/140‑1 (MQ=255)
                                                         cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                  <  2:2837935/140‑1 (MQ=255)
                                                               ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCAATCAGTCaa                                                                            >  1:764021/1‑140 (MQ=255)
                                                                tttCAATAGAAATCTGTATATTCCGCTTTATCAGCTGATTTATCGCTATTACAAAGAAAACATGCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGACAGCTTACCATCAGTCaaa                                                                           <  2:787723/140‑1 (MQ=255)
                                                                 tttaaTATAAATCTGGATATTCCGCTTTATCAGCAGATTTATCGCTATTTCAAACAAAACATGCATCAGGGGCGTATTCAAAAAGGATTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                          <  2:2896686/137‑1 (MQ=255)
                                                                 tttaaTAGAATTCTGGATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACATGCATCAGGGGCTTATTCAAAAAGCAATTAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGATTTCCATCAGTCacac                                                                          <  2:575888/137‑1 (MQ=255)
                                                                 ttGAATATAATTCTGGATATTCCGATTTATCAGCAGCTTTATCGCTATTACAAATAAAACATGCATCAGGGGCTTATACAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGTGTCAGATTTCCATCAGTCAAAc                                                                          <  2:2870097/140‑1 (MQ=255)
                                                                   gAATACAAATCTGGATATTCCTCTTTATCAGCAGGTTTATCGCTATTTCAAATAAAAAATGCATCAGGGGCGTTTTAAAAAAGGATTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTAAGGTTGCCATCAGACAAACGa                                                                        <  2:1183004/140‑1 (MQ=255)
                                                                      tctaaaTCTGGATTTTCCGCTTTATCAGCAGCTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCTTATACAAAAAGGAATGAAGCGCCCTTCCAAACGACTCCTGGCGAGTCAGCTTCCCATCAGACACACGACTg                                                                     <  2:3433905/137‑1 (MQ=255)
                                                                             ctgtttatTCCTATTTAAAAGCAGCTTTATCGATATTAAAAAGAAAACATCAATCCGGGGCTTATACAAAAAGTAATGAAGCTCCCTACAAAAACACTCCTGGCGAGTCAGATTTCCAACAGTCAAACGACTGTAGAACg                                                              <  2:2551710/135‑1 (MQ=255)
                                                                             cTGTATATTCCTCTTTATCAGCAGCTTTATCGATATTTAAAAGAAAAAATGCAACAGGGGCTTATTAAAAAAGTAATGACGCTCCCTTCAAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                              <  2:1493619/140‑1 (MQ=255)
                                                                             cTGGATTTTCCGCTATATCAGCATCTTTATCGCTTTTTCAATGAAAACATGCATCCGGGGCTTATTCAAACAGCAATGAAGCTCCCTTCAAACACACTCCCCGCGAGAAAGCTTTCCATCCTTCAAACGACTGTAGAACg                                                              <  2:1674662/140‑1 (MQ=255)
                                                                             cTGGATATTCCGCTTTATCAGCCGCTTTATCGCTATTTAAAATAAAACATGCATCAGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                              <  2:165628/140‑1 (MQ=255)
                                                                              tGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGt                                                             >  2:3128115/1‑140 (MQ=255)
                                                                               ggATATTCCGCTTTATCAGCAGCTTTATCGATTTTACAAACAAAAAATGCATCAGGGGCTTATTAAAAAAGTAATTAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTg                                                            <  2:445988/140‑1 (MQ=255)
                                                                                       cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaa                                                    >  2:2429837/1‑140 (MQ=255)
                                                                                        gCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGact                                                   >  1:2428614/1‑138 (MQ=255)
                                                                                            tATCAGCAGCTTTATCGCTATTACAAATAAAACATGCATCCGGGGCTTATTAAAAAAGCAATGAAGCTCCCTTCAAAAACCCTCCTGGCGAGTCAGCTTACCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                               <  2:12734/140‑1 (MQ=255)
                                                                                              ccaccagcATTTTCGCTATTTAAACCAAAACATGCATCGGGGGCTTATTCAAAAATCAATCAAGCTCCCTCCAAAAAGCCTCGTGGCGCTCCAGCTTCCCACCACTCCCACGACTGTAGAACGTGCCTATGAACATCTtg                                             <  2:467302/136‑1 (MQ=255)
                                                                                                   ttgtttATCGATATTTCAAACAAAACCTGAATCCGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCAAAAAGACTCCGGCCGAGTCAGCGTACCAACAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  1:2375388/137‑1 (MQ=255)
                                                                                                   tgttttAGCGCTATTTCAAAGAAAACAGGCATCCGGGGCGTATTAAAAAAGGATTGAAGCTCCCTTCCAAACGACTCCTGGCGAGTCAGCTTGCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  2:2245583/137‑1 (MQ=255)
                                                                                                   atttttATCGCTATTTCAAACAAAACAGGCATCTGGGGCTTATTAAAAATGAAATGACGCGCCCTTCAAAAACACTCCTGGCTTGTCAGATTTCCATCTTACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  1:484573/137‑1 (MQ=255)
                                                                                                   agcTTTCTCGCTATTACAAAGAAAACATGCATCAGGGGCTTATTCAAAATGGAAGTAAGAGCGCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  2:504346/140‑1 (MQ=255)
                                                                                                   agcTTTATGGCTATTACAAAGAAAACCTGCATCAGGGGCTTATTCAAAAAGTAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  2:1642348/140‑1 (MQ=255)
                                                                                                   agcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  2:2043720/140‑1 (MQ=255)
                                                                                                   agcTTTATCGATATTAAAAATAAAACATGCAACCGGGGCTTATCAAAAAAGGAATCACCCCCCCTCCAAAAACACGCCGGGCGAGTCAGCTTCCCAACAGCCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  1:1033623/140‑1 (MQ=255)
                                                                                                   agcTTTAGCGATATTACAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTGCCAAAAGCCTCCTGGCGATTCAGCTTTCCATCTGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                        <  2:1540680/140‑1 (MQ=255)
                                                                                                     ttttATCCTTATTTCAAATAAAACATGCATCAGGGGCTTATTCAAAAATCAATGAAGCTCCCTTCCAAAAGACCCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:2986116/139‑1 (MQ=255)
                                                                                                     gtttAGCGCTTTTTCAAAGAAAACATGAATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCGTCCAAAACACTCCTGGCGAGTCAGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:2768929/139‑1 (MQ=255)
                                                                                                     cTTTATCTCTATTTAAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCACCCTTCCAAAAGACTCCTGGCGAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:3041471/140‑1 (MQ=255)
                                                                                                     cTTTATCGATATTTCAAATAAAACAGGCATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCATCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:2472548/140‑1 (MQ=255)
                                                                                                     cTTTATCGATATTTAAAATAAAACATGCATCCGGGGCTTATTCAAAAAGGAATGAAGCTCCCTACCAAACTACTCCTGGCGTGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:1740725/140‑1 (MQ=255)
                                                                                                     cTTTATAGCAATTTCAAATAAAACATGAATCTGGGGGGTATACAAAAATTTTTCAAGCTCACTTCAACAACACGCCGGGGGATTCAGCTTCCCATCCCTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  2:2951819/140‑1 (MQ=255)
                                                                                                     cTTTAGCGCTATTACAAACAAAACAGGCATCCGGGGCGTTTAAAAAAAGGAAGGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                      <  1:2277111/140‑1 (MQ=255)
                                                                                                        taccccTATTAAAAACAAAACATGCATCCGGGGCTTATTAAAAAAGTAATGACGCCCCCTTCAAAAAGCCTCCTGGCTTTTAAGATTACCATAAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                   <  1:314272/135‑1 (MQ=255)
                                                                                                        tATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAggg                                   >  1:2109273/1‑140 (MQ=255)
                                                                                                          tCGCTATTTAAAATAAAACATCCATCAGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                 <  2:3213634/140‑1 (MQ=255)
                                                                                                          tCGCTAGTACACACAAACCATGAAGCCGGGGCCTAAACAAAACGGCCTGAAGCTCCCTACCAACACACACCTGGCGCGTCAGCTTTCCAACAGTCAAACGACTGTAGAACGTGCCTCTGAACAGCTTGCTGCTGAGGGCt                                 <  2:1878260/140‑1 (MQ=255)
                                                                                                          tCGATATTTCAAATAAAACCTGCATCCGGGGCTTATTCAAAAAGGACTGAAGCTCCCTTCCAATTGACTCCGGGGGTGTCAGCTTTCCATCTTACAAACGACTGTAGAACGTGCCTATGAAGAGCTTGCTGCTGAGGGCt                                 <  2:108593/140‑1 (MQ=255)
                                                                                                          acGCTATTACAACCAAAACAGGCATCCGGGGCTTATACAAACCGCAATGAAGCGCCCTTCCAAACGACCCGGGGCCCGTCAGCTTCCCAACAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                 <  1:643008/139‑1 (MQ=255)
                                                                                                               aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTAtctc                            >  1:703506/1‑140 (MQ=255)
                                                                                                               aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                            >  2:752249/1‑140 (MQ=255)
                                                                                                                tttCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGTCTATATct                           >  1:1743276/1‑139 (MQ=255)
                                                                                                                     aataaaaCATGCTTCCGGGGCGTATTAAAAAAGGAATTAAGCTCCCTCACAAAAGACTCCTGGCGAGTCAGCTTTCCAACAGTAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                      <  2:2826049/137‑1 (MQ=255)
                                                                                                                     aaGAAAACATCCATCAGGGGCTTATACAAAAAGTAATTAAGCCCCGTTCCAAAACACTCGGGGCGAGTCAGATTTCCATCACTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                      <  1:1374367/140‑1 (MQ=255)
                                                                                                                          aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                 >  1:58692/1‑140 (MQ=255)
                                                                                                                          aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTAAGCTTTCCATCAGACAAACGACTGTAGACCGTGCCTATGAACAGCTGTCTGCTGAGGGGTCTCTCGTGAGCACGcc                 >  2:2581122/1‑140 (MQ=255)
                                                                                                                            caggCATCCGGGGGGTTTTAAAAAAGGAATCACGCTCCCTTCCAAACCCGCCGTGGCCAGGAAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa               <  2:2075623/137‑1 (MQ=255)
                                                                                                                            cATGCATCAGGGGCGTATTCAAAAAGGAATTAAGCGCCCTTCAAACACACTCCTGGCTAGTCAGCTTTCAATCCTTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa               <  2:939380/140‑1 (MQ=255)
                                                                                                                            cATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa               <  2:2436407/140‑1 (MQ=255)
                                                                                                                            cATGAATCCGGGGCGTATAAAAAAAGTAATCACCCCCCCTACCAAACCCCTCCGGGCGAGTCAGGTTTCCACCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaa               <  1:3128115/140‑1 (MQ=255)
                                                                                                                             aTGAATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCGTGGCGTGTCAGGTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaag              <  1:2873342/140‑1 (MQ=255)
                                                                                                                              tgaaTCTGGGGCTTATAAAAAAAGCAATGAAGCTCCCTTCCAACAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaaga             <  1:3367578/137‑1 (MQ=255)
                                                                                                                              tGCATCAGGGGCGTATTCAAAACGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCaaga             <  1:2535831/140‑1 (MQ=255)
                                                                                                                                      gggCGTATTCAAAAAGCAAGGAAGCTCCCTCCCAAAACACTCCTGGCGCGTCAGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATg     <  2:17834/140‑1 (MQ=255)
                                                                                                                                         cGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGtt  >  2:2975122/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AGCCAGTTTTCACAAAAAAATGCATACCAGTTAGGAGAACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTT  >  NZ_CP034943/548942‑549218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑