Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0540CG28.8% 263.8 / 38.2 143E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (28/71);  new base G (39/1);  total (71/72)
Fisher's exact test for biased strand distribution p-value = 5.63e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 148 total aligned reads displayed.

CATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATC  >  NZ_CP034943/633919‑634192
                                                                                                                                       |                                                                                                                                           
cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttcttt‑c                                                                                                                                        >  2:3398474/1‑140 (MQ=255)
cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGgtgtttg                                                                                                                                         >  1:2774784/1‑139 (MQ=255)
 atggatggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttcttt‑ct                                                                                                                                       <  2:2126012/140‑1 (MQ=255)
  tggatggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGtgttt‑gtt                                                                                                                                      >  2:3365234/1‑140 (MQ=255)
      tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTCAGGTTTTCTTCCATGGTTTGAACAGTGGCGTCAATTTCTTCAATCCGACGGAGTCGTTTGCGGTCTTT‑Cgtttgc                                                                                                                                  >  2:3279716/1‑138 (MQ=255)
        gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑GTTTTTGCa                                                                                                                                >  2:2140348/1‑140 (MQ=255)
        gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGATTT‑CTTTTTACa                                                                                                                                >  2:402859/1‑140 (MQ=255)
        gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTGATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTT‑CTTGTTCCa                                                                                                                                >  2:3412571/1‑140 (MQ=255)
          ttGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTT‑CTTTTTGCAtt                                                                                                                              >  2:2739798/1‑140 (MQ=255)
          ttGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTT‑ATTTTTGCAtt                                                                                                                              >  2:1242092/1‑140 (MQ=255)
             tACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTT‑CTTTTTTCaatgat                                                                                                                           >  1:132731/1‑135 (MQ=255)
                  tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCACTGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGCTTT‑ATTTTTGCAGTCtttttat                                                                                                                      >  2:2449533/1‑138 (MQ=255)
                  tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTT‑GTTTTTCCATTAtttttat                                                                                                                      >  2:3181128/1‑138 (MQ=255)
                  tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAAGGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTAGTT‑CTTTTTACAATCTTTttct                                                                                                                      >  2:2950433/1‑140 (MQ=255)
                   ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGGTGTTT‑CTTTTTACAATATTTttgtt                                                                                                                     >  2:305960/1‑140 (MQ=255)
                   ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTATTT‑GTTTTTCCATTGTTTttatt                                                                                                                     >  2:309686/1‑140 (MQ=255)
                    tCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGGTTT‑CTTTTTACATTGTTTttgttg                                                                                                                    >  2:2161583/1‑139 (MQ=255)
                    tCATGGTATTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTttcttc                                                                                                                    <  2:3357417/140‑1 (MQ=255)
                       tGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑ATTTTTACATTATTTttattcttc                                                                                                                 >  2:3166678/1‑140 (MQ=255)
                       tGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGGTTT‑TTTTTTACAGTCTTTttattattg                                                                                                                 >  2:1116999/1‑139 (MQ=255)
                             tGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGa                                                                                                           <  1:208929/140‑1 (MQ=255)
                              gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                          <  1:1142550/140‑1 (MQ=255)
                              gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                          <  1:1142572/140‑1 (MQ=255)
                              gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                          <  1:2305009/140‑1 (MQ=255)
                               aTAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTT‑GTTTTTGCATTCTTTTTCTTCTTAATATGAAc                                                                                                         >  2:439501/1‑140 (MQ=255)
                               aTAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAAACCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTAAATTATTTTTATTGTTAAAATGAAc                                                                                                         >  2:2998334/1‑140 (MQ=255)
                                  gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                      <  1:2550107/140‑1 (MQ=255)
                                  gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                      <  2:204104/140‑1 (MQ=255)
                                      tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑CTTTTTACATTCTTTTTATTATTCATATGAAAGTTTTGa                                                                                                  >  2:2808920/1‑140 (MQ=255)
                                      tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑CTGTTTGCAATATTTTTCTTCTTCATATGAACGTTTTGa                                                                                                  >  2:1363126/1‑140 (MQ=255)
                                      tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑ATTTTTCCATTATTTTTATTCTTAATATTAAACTTTTgt                                                                                                  >  2:1750176/1‑139 (MQ=255)
                                       ccGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAg                                                                                                 <  2:1479065/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTACTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                <  1:1932488/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCCTATGAACGTTTTGAGt                                                                                                <  1:2311759/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                <  1:1510180/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                <  1:39732/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                <  1:2950433/140‑1 (MQ=255)
                                        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                <  1:3181128/140‑1 (MQ=255)
                                        cGGATCAAATATCAATTCATCGTTATGACTTTTGTTTTCTTCAATGGTTTGAACTGTTGCTTCAATTTCTTCAATCCGTCGGAGTCGTTGGGGTTGTTT‑GTTTTTCCTTTCTTTTTCTTGTTCATCTGAACTTTTTGAGt                                                                                                <  2:2373493/140‑1 (MQ=255)
                                         ggATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTc                                                                                               <  1:2741818/140‑1 (MQ=255)
                                                  aGCAATTCATCGTTATGACTAATGTGTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                      <  1:2161583/140‑1 (MQ=255)
                                                      aTTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑GTTTTTGGATTGTTTTTGTTGTTGATTTTTACGTTTTTTGTGATGGTTTACCGaa                                                                                  >  1:2454767/1‑140 (MQ=255)
                                                        tCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTT‑GTTTTTGGATTGTTTTTGTTGTTGATATGAAAGTTTTTTTTCAGGCTTaaaccaacc                                                                                >  1:280159/1‑133 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑CTTTTTCCATTCTTTTTATTATTCATATGAAAGTTTTTAGTAAGGCTTAACCGAAACa                                                                               >  2:1290288/1‑140 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTGT‑CTTTTTCCATTATTTTTCTTCTTCCTATGAACGTTTTGAGTCAGGCGTAACCGAAGCa                                                                               >  2:221813/1‑140 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTT‑CTTTTTCCCTTATTTTTCTTCTTCATATGAACGGTTTGAGTGAGGCTTAAACGAAGCa                                                                               >  2:361313/1‑140 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTTTGTAAGGCGTAAACGAAGCa                                                                               >  2:2272452/1‑140 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTT‑GTTTTTCCATTATTTTTCTTGTTTGTATGAAAGGTTTGATTCAGGGTTAAACCAAGCa                                                                               >  2:614837/1‑140 (MQ=255)
                                                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTT‑CTTTTTGGATTCTTTTTGTTCTTCAAATGAACCTTTTGAGTAAGGCTTAAACGAAGCa                                                                               >  2:1117241/1‑140 (MQ=255)
                                                          aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTCGTT‑GTTTTTCCATTCTTTTTCTTCTTCATATTAACGTTTTGAGTCAGGCTTAACCGAAGCAg                                                                              >  2:427496/1‑140 (MQ=255)
                                                            cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑GTTTTTGCATTGTTTTTCTTCTTAAAATGAACGTTTTGAGTAAGGCGTAAACGAAGCAGGc                                                                            >  2:1549324/1‑140 (MQ=255)
                                                              ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTT‑CTTTTTACATTCTTTTTGTTCTTAATATGAACGTTTTTAGTCAGGCTTAAACGAAGCAGGCGt                                                                          >  2:133512/1‑140 (MQ=255)
                                                                   aCTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTCTTT‑ATTTTTCCATTCTTTTTATTCTTCATATTAAAGTTTTGAGTCAGGCTTAACCGAAGCAGGCGtttttt                                                                     >  2:2304153/1‑140 (MQ=255)
                                                                     tAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCg                                                                   <  2:2204140/140‑1 (MQ=255)
                                                                      aaTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCgg                                                                  <  2:1203518/140‑1 (MQ=255)
                                                                        tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACCAAGCAGGCGTTTTTTAGGAt                                                                >  2:819154/1‑140 (MQ=255)
                                                                            ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGCCGGAGTCGTTGGCGTTCGTT‑CTTTTTCCATTCTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCct                                                            <  1:3279716/140‑1 (MQ=255)
                                                                            ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGCTTT‑CTTTTTGCATTGTTTTTCTTCTTCATATTAACGGTTTTAGTGAGGCTTAACCGAAGCAGGCGTTTTTTCggggtcga                                                            >  2:3032180/1‑134 (MQ=255)
                                                                             tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGGTT‑GTTTTTGCAGTTTTTTTGGTGTTGATATTTACGTTTTTTGTAAGGGGTTAACGAAACAGGGGGTTTTTGGGGttgcag                                                           >  1:1636409/1‑136 (MQ=255)
                                                                             tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTTCCATT‑TTTTTGTTGTTGAGATGGACGGTTTTTGTGAGGGGTTAACGAACCAGGCGGGTTTTGGGttttcgg                                                           >  1:223948/1‑136 (MQ=255)
                                                                                 cAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑CTTTTTGCATTGTTTTTCTTCTTAATTTGAAGGTTTTGAGTCAGGGGTAACCGAAGAAGGCGTTTTTTGGGATTCCTCTTgg                                                       >  2:3007051/1‑139 (MQ=255)
                                                                                        tttaaCAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCa                                                <  2:2126743/137‑1 (MQ=255)
                                                                                        ttGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCa                                                <  2:2526353/140‑1 (MQ=255)
                                                                                         tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGGTTT‑GTTTTTGCATTCTTTTTGTTATTGAGTTTAACGTTTTTTGTCAGGCTTTACCGAAGCAGGCGTGTTTTGGGATTGCGCTTGGTGTTTGAt                                               >  1:3315181/1‑140 (MQ=255)
                                                                                          gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                              <  1:1636589/140‑1 (MQ=255)
                                                                                              aGTGTCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CATTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGATTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                          <  2:685267/140‑1 (MQ=255)
                                                                                              aGTGGCTTCAATTTCTTGAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTGTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                          <  2:136870/140‑1 (MQ=255)
                                                                                              aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                          <  2:2515684/140‑1 (MQ=255)
                                                                                               gTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                         <  2:2913394/140‑1 (MQ=255)
                                                                                               gTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                         <  2:280159/140‑1 (MQ=255)
                                                                                               gTGGCTTCAATTTCTTCAATACGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtctc                                         <  2:601709/140‑1 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTT‑CTTTTTACATTCTTTTTGTTTGTCATATTAACGTTTTGATTGAGGCTTAAACGAAACACGCGTTTTTTAGGATTTCTCTTTGTGATTCAttttatga                                        >  2:2898375/1‑136 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTT‑GTTTTTGCATTCTTTTTCTTGTTCATATGAACGTTTTTAGTCAGGCTTAACCGAAGCAGGCGTTTTTTGGGTTTGCTCGTTCTGTTTGATTTTGTcc                                        >  2:2352725/1‑140 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑GTTTTTCCATTCTTTTTCTTCTTAATATGAACGTTTTTATTGAGGCTTTAACGAACCAGGCGTTTTTTAGGATTACGCTTTCTGTTTAATTTtctgc                                        >  2:1636589/1‑138 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                      <  1:1539806/78‑1 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑ATTTTTGCATTCTTTTTCTTATTCATATGTACGTTTTGAGTCAGGGTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGGTGTTTAATTTTCTcc                                        >  2:2466776/1‑140 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGTGGTTT‑GTTTTTACCTTCTTTTTATTCTTCATATGAACCTTTTTAGTCAGGCTTAAACCAAGCAGGCGTTTTTTAGGATTCCTCATGCTGATTCAttttatac                                        >  2:2640138/1‑136 (MQ=255)
                                                                                                tGGCTTCAATTTCTTCAATCCCACGGAGTCGTTGGCGGTATTT‑AGTTTTACATTATTTTTATTCTTCATATTAACGTTTTTAGTCAGGCATAAACCAACCAGTCGTTGTTTGTGATTCCCATTTCTGAATCATTTTCTcc                                        >  2:3318213/1‑140 (MQ=255)
                                                                                                  gCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGGGGTT‑GTTTTTTCAATGTTTTTGTTGTTGATATGTACCGTTTGAGTGAGGGTTTACCCCAGCCGGGGTGTTTTAGGTTTGGGCTTGGGGAGTGCTTTTCTCCaa                                      >  1:1379235/1‑140 (MQ=255)
                                                                                                        aTTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                                <  1:3127886/140‑1 (MQ=255)
                                                                                                             ttcAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                           <  2:1566423/140‑1 (MQ=255)
                                                                                                             ttcAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                           <  2:309262/140‑1 (MQ=255)
                                                                                                                 aTCCGACGGAGTCGTTGGCGTTGTTT‑CTTTTTCCCGTCTTTTTCTTCTTCATATGAACGTTTTGTGTGAGGGTTAACCGAAGCAGGGGGTTTTTCGGATTCCGCGTGGTGTGTCATTTTCTCCAATTCAAGCTGctcctg                       >  2:1611202/1‑137 (MQ=255)
                                                                                                                       cGGTGTCTTTGTAGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:2690223/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:2998334/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  2:821403/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:1238974/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:1363126/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:2466776/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:439501/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:681295/140‑1 (MQ=255)
                                                                                                                       cGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                 <  1:807087/140‑1 (MQ=255)
                                                                                                                         gAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTACTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct               <  1:2508665/140‑1 (MQ=255)
                                                                                                                         gAGTCGTTGGCGTTCTTT‑CTTATTCCAGACTTTTACTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct               <  1:3412571/140‑1 (MQ=255)
                                                                                                                          aGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtctc              <  1:3009762/140‑1 (MQ=255)
                                                                                                                          aGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtctc              <  1:1276534/140‑1 (MQ=255)
                                                                                                                            tctttGGCCGTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGCACGTTTTGAGTCACGCTATACCGAATCAGTCGTTTTTTCGGATTGCTCTTGCTGATTCATTTTCTCCAATTCACCCTGATCTGTTTTTTTCTCCg            <  2:1955566/137‑1 (MQ=255)
                                                                                                                            tCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg            <  1:819154/140‑1 (MQ=255)
                                                                                                                            tCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg            <  2:1459479/140‑1 (MQ=255)
                                                                                                                               ttGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGtat         <  2:67886/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
CATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTT‑CTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATC  >  NZ_CP034943/633919‑634192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑