Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6490CA26.1% 295.1 / 21.1 149P582T (CCG→ACG) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (21/84);  new base A (37/0);  total (65/84)
Fisher's exact test for biased strand distribution p-value = 1.85e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 149 total aligned reads displayed.

GCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGA  >  NZ_CP034943/1111513‑1111785
                                                                                                                                        |                                                                                                                                         
gcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGc                                                                                                                                        <  1:1298176/140‑1 (MQ=255)
 caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGCGGAGTTTGTTGAACTGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATGTTATCTAATTCCGcc                                                                                                                                       >  1:1732953/1‑140 (MQ=255)
   tCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCCTTTTTTCTAATTAAGCCTg                                                                                                                                     >  1:2251227/1‑140 (MQ=255)
    cagcagGCGTGGGATGTAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgc                                                                                                                                    <  2:366238/140‑1 (MQ=255)
      gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                                  <  1:2508069/140‑1 (MQ=255)
      gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                                  <  1:91159/140‑1 (MQ=255)
      gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATATTCTCAAATAGCGCCAgcgc                                                                                                                                  >  2:453555/1‑140 (MQ=255)
      gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTATGTAATTGCGCCTgcgc                                                                                                                                  >  2:1471311/1‑140 (MQ=255)
      gcagGCCTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                                  <  2:2048446/140‑1 (MQ=255)
           cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTAGATAATTAAGCCTGCGC‑TTTAc                                                                                                                            >  2:1599925/1‑140 (MQ=255)
               ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAgg                                                                                                                        <  1:3187206/140‑1 (MQ=255)
                 aTGCAGTCATTCAGCTACTTGAGGAGTTTGTTGACATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACCTTTTCTCTAATTCCGCCTGCGC‑TTGACCAGgtg                                                                                                                      <  2:1764321/140‑1 (MQ=255)
                    cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTCTAATTGAGCCGCCGC‑CTGACCACGTGttt                                                                                                                   >  1:3326502/1‑140 (MQ=255)
                    cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATATAATTACCCCGGCCC‑TTTACCAGGTGgtt                                                                                                                   >  2:1888429/1‑140 (MQ=255)
                    cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAAGTTTTTATAATTACCCCCGCGC‑GTGTCCAGGTGgtt                                                                                                                   >  1:1392383/1‑140 (MQ=255)
                         actCAGCTACTTGAGGAGTGTGTTGAAATGATGGGGGCTGCTGAGTTTTCTCGTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCgg                                                                                                              <  1:2922519/138‑1 (MQ=255)
                         aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCgg                                                                                                              <  1:642433/140‑1 (MQ=255)
                         aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCgg                                                                                                              <  2:2753567/140‑1 (MQ=255)
                         aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTGATTTTATGTAATTACGCCAGCGC‑GTTTGCAGGTGGTTGTggg                                                                                                              >  1:1491857/1‑140 (MQ=255)
                         aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTGCTCTTGAGTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTGTGATTCCGCCTGGGC‑TTGTCCAGGTGGTTGTggg                                                                                                              >  2:59890/1‑140 (MQ=255)
                          ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTTTATAATTCCGCCTGCGC‑GTGACCCGGTGTTTTTGGGc                                                                                                             >  2:3139946/1‑140 (MQ=255)
                          ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTGTGTAATTACGCCAGCGC‑GTGTCCAGGTGTTTTTGGGc                                                                                                             >  1:2376092/1‑140 (MQ=255)
                          ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATATAAATACGCCAGCGCGTTTACC‑GGGGTTTGTGGGc                                                                                                             >  1:2164189/1‑140 (MQ=255)
                          ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTGTCTAATTACGCCGGCGC‑TTGACCAGGTGTTTgggggc                                                                                                             >  2:3131977/1‑135 (MQ=255)
                          ttCAGCTACTTGAGGAGTTTGTTGAAATGAGGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGAAGGAAGCCGGCGCGGAGTCGCGTACATTTTTTCTTAGTACCCCGGGGC‑TTGAACAGGTGttttggggc                                                                                                             >  2:1252294/1‑134 (MQ=255)
                              gCTACGTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAata                                                                                                         <  1:65209/140‑1 (MQ=255)
                               cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAGTTTATCTAAATACGCCTGCGC‑TTTACCACGTGTTTTTCGGGAaaat                                                                                                        >  2:2782449/1‑140 (MQ=255)
                               cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTGTCTAATTACCCCAGCGC‑GTGACCACGGGTTTGTTGGGAatat                                                                                                        >  1:1815369/1‑140 (MQ=255)
                               cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAGATTTTTTCTAAATAAGCCAGCGC‑GTGAGCAGGTGTTTTTGGGGAatat                                                                                                        >  1:121106/1‑140 (MQ=255)
                               cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTAAGTAAATCCCGCTGAGC‑GTTACAACGTGTTTGTAGGCAaaat                                                                                                        >  2:2258355/1‑140 (MQ=255)
                                 aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGTAAATTTTTTTGTCGGCAATATgg                                                                                                      <  1:2460299/140‑1 (MQ=255)
                                 aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTATCTAATTACCCCTGCGC‑GTGACCACGTGGTTTTGGGCaaaaagg                                                                                                      >  1:1771598/1‑135 (MQ=255)
                                 aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAATTTTGTGTAATTAAGCCTGCGC‑GTGACCACGTGTGTTTGGGGAatattg                                                                                                      >  1:3007068/1‑138 (MQ=255)
                                  cTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGa                                                                                                     <  2:383674/140‑1 (MQ=255)
                                  attGAGGCGTTGGGTGACATGTTGGGGTATGATGAGATTTCTCTTGATTTGTTTCAGCACATGATGGAAGCCGGCGCGGCGTCGCGTACATTTTCTCTAATTCAGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGa                                                                                                     <  2:2636019/139‑1 (MQ=255)
                                   ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                    <  2:1950842/140‑1 (MQ=255)
                                        gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTGTAGAAATACGCCTGCGC‑TTGACCACGTTTTTTTAGGCAATATGGAgtttta                                                                                               >  2:2460299/1‑137 (MQ=255)
                                         agTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATcc                                                                                              <  1:1324201/140‑1 (MQ=255)
                                         agTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATcc                                                                                              <  2:3023233/140‑1 (MQ=255)
                                         agTTTGTTGAAATGATGGGCGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATcc                                                                                              <  1:2332440/140‑1 (MQ=255)
                                          gTTTGTTGAAATGATGGTGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                             <  1:1275308/140‑1 (MQ=255)
                                          gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                             <  1:2812429/140‑1 (MQ=255)
                                          gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                             <  1:1275186/140‑1 (MQ=255)
                                          gTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCa                                                                                             <  1:2009745/140‑1 (MQ=255)
                                            ttgttgCAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                           <  2:2732204/140‑1 (MQ=255)
                                            ttgttgAAATGATGGGGGATGTTGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                           <  2:2438496/140‑1 (MQ=255)
                                            gggttgAAATGATGGGGGATGATGAGATTTCTCTTGAGTTGGTTCAGCAAATGATGGAAGCCGGCGCGGAGGCGCTTACATGTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTGTGTCGGCAATATGGATTTATCCAGa                                                                                           <  2:3228170/138‑1 (MQ=255)
                                                   aaTGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAAATACGCCAGCGC‑GTGACCACGGGTTTGTGGGCAAAAGTGATTTATACAGaaattaag                                                                                    >  2:3073327/1‑134 (MQ=255)
                                                   aaTGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTCTAATTCAGCCTGCGC‑GTGTCCACGTGTTTTTGGGGCAAAAGGATTTATTAAGAAAGTaag                                                                                    >  1:670023/1‑138 (MQ=255)
                                                   aaTGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTCTCTAATTCAGCCTGAGC‑GTGACCAGGTGTTTGTGGGCAATATGGTGTTATACAGAATGTaag                                                                                    >  2:1834474/1‑138 (MQ=255)
                                                   aaTGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGCAGTCGCTTTCATTTTATATAAATACCCCAGAGC‑GTGACCAGCTGTTTTTAGGCAAAATTGAATTATACACAAtataag                                                                                    >  2:922615/1‑138 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  1:1870349/140‑1 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  2:593687/140‑1 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  1:3094816/140‑1 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  2:399122/140‑1 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  1:1214816/140‑1 (MQ=255)
                                                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGAAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                  <  1:1084510/140‑1 (MQ=255)
                                                        tgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCAcc                                                                               <  2:1063003/140‑1 (MQ=255)
                                                         gggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCAATTTATGTAAATAAGCCGGCGG‑GTGAAACCGTGTTTTTGGGCCAGATTGATTTTTTAACAATATACGGCAcca                                                                              >  1:2822921/1‑139 (MQ=255)
                                                            ggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTATATAAAAAAGCCAGCGC‑TTTTAAACGTGTTTTTAGGCAAAATTTATTTTTCCAGAAATTAAGGGACCACtt                                                                           >  2:1430039/1‑140 (MQ=255)
                                                             gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                          <  2:306442/140‑1 (MQ=255)
                                                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                        <  2:669939/140‑1 (MQ=255)
                                                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                        <  2:964179/140‑1 (MQ=255)
                                                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                        <  1:1430039/140‑1 (MQ=255)
                                                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                        <  1:2581221/140‑1 (MQ=255)
                                                                  tgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCt                                                                     <  1:1485621/140‑1 (MQ=255)
                                                                   gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                                    <  2:2227084/140‑1 (MQ=255)
                                                                     gaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCAATTTTTATAAATAGGCCGCGGG‑GTGAGCAGGTGTTTTTGGGCAAAATTGATTTATGAAGCATTTAAGGCACCACATGGAccaagt                                                                  >  1:3316748/1‑136 (MQ=255)
                                                                      aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTTTGTAAATAAGCCAGCGC‑GTGACCAGGTGTTTTTTGGCCAAATTGATTTATACACAAAGTAAAGCACCACTTTCACCgtttt                                                                 >  1:1720240/1‑138 (MQ=255)
                                                                                aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTGTAGAATTAAGCCGGCGC‑GTGTACACGTGGTTTTTGGGCATATTTATTTATAAAGAAAGTACGGCACCACGTTGACCGTTGTGCGGGGGGcc                                                       >  1:399346/1‑139 (MQ=255)
                                                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCAATTTTTATAATTACGCCAGCGC‑TTGACCACGGGTTTGTGGGGAATATTGATTTTTCCAGAATTTACGGCACCTCTTGGACCTTTTTGGTGGGGGCAAacca                                                  >  2:1546885/1‑138 (MQ=255)
                                                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                                  <  2:2164189/140‑1 (MQ=255)
                                                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                                  <  2:1267573/140‑1 (MQ=255)
                                                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                                  <  2:2376092/140‑1 (MQ=255)
                                                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTGTAATTTTGGGTAATTAAGCCGGCGC‑GTTGCCAGGTGTTTGTTGGCACAATGGGTTTATACAGAAAGTAAGGCCCCGCGTGCACCGTTTTTCTggggggccaaga                                                  >  2:274725/1‑133 (MQ=255)
                                                                                     gtgcAGCAAATGATGGAAGCCGGCGCGGCGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAAGGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacga                                                  <  2:2181516/137‑1 (MQ=255)
                                                                                      ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                                 <  1:594425/140‑1 (MQ=255)
                                                                                         aGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTGTCGAATTGCCCCGGCGC‑TTGACCAGGGGTTTTTGGGCAATATGGGTTTTTCCAGAATTTAAGGCACCTCTTGGACCGTTGTGGTGGGGGCAAAAGACCGc                                              >  2:2483193/1‑140 (MQ=255)
                                                                                          gCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCg                                             <  2:2034734/140‑1 (MQ=255)
                                                                                          gCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCg                                             <  2:3259721/140‑1 (MQ=255)
                                                                                           cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                                            <  2:1969797/140‑1 (MQ=255)
                                                                                            aaaTGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGtt                                           <  2:2388451/140‑1 (MQ=255)
                                                                                             aaTGATTGAAGACGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGATTGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTc                                          <  2:481536/140‑1 (MQ=255)
                                                                                             aaTGATGGAAGCCGGCGCTGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTc                                          <  2:656215/140‑1 (MQ=255)
                                                                                                gatgGAAGCCGGCGCGGAGTCGCTTACATTTTATCTAATTCCGCCTGCGC‑TTTAACAGGTGTTTGTGGGCAATATGGATTTATCCATAATTTACGGCCCCTCTTGGACCGTTGTGGGGGGGGGCAAAGAAGGCGTTctgc                                       >  2:1918342/1‑140 (MQ=255)
                                                                                                    gAAGCCGGAGCGGAGTCGCTTACATTTTCTATAATTCCGCCTGCGC‑TTGACCCGGTTTTTGTCGGCAATAGGTATTTATCCAGAATGTACGGCACATCTGGCACATTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                                   <  1:364368/140‑1 (MQ=255)
                                                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                              <  1:2668412/140‑1 (MQ=255)
                                                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                              <  1:1888124/140‑1 (MQ=255)
                                                                                                             gcgGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCtga                          <  1:297344/140‑1 (MQ=255)
                                                                                                                gactCGCTTACATTTTCTCTAAGTCCGCCTGCGC‑TTGACCAGTTGTTTGTCGGCAATATGGATGTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGTGCAAACGACGGCGTTCTGCCTGCACGCCCtgatga                       <  1:290844/137‑1 (MQ=255)
                                                                                                                   tCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa                    <  2:1302509/140‑1 (MQ=255)
                                                                                                                          cATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg             <  1:1995865/140‑1 (MQ=255)
                                                                                                                             tttCTCTAATTCCGCCTGCGC‑GTGACCCGGTGTTTGTCGGCACTCTGGGTTTATCCAGCATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGTCGGCGTGCTGGCGGCAGGCCCTGATGTAACTGGGGTGCTg          >  2:2818120/1‑140 (MQ=255)
                                                                                                                               tctcTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGGTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGCACATGGGGTGCTGTc        >  2:230906/1‑140 (MQ=255)
                                                                                                                                 tctAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg      >  2:2893088/1‑140 (MQ=255)
                                                                                                                                   tAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                          <  1:385841/70‑1 (MQ=255)
                                                                                                                                    aaTTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACg   <  2:399346/140‑1 (MQ=255)
                                                                                                                                     aTTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGa  >  1:2617688/1‑140 (MQ=255)
                                                                                                                                     aTTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGa  >  1:261329/1‑140 (MQ=255)
                                                                                                                                     aTTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGa  >  1:44568/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
GCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGC‑TTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGA  >  NZ_CP034943/1111513‑1111785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑