Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0960GC32.2% 178.6 / 36.6 122E173Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (46/34);  new base C (0/38);  total (48/74)
Fisher's exact test for biased strand distribution p-value = 3.48e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 122 total aligned reads displayed.

AAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGT  >  NZ_CP034943/1729964‑1730223
                                                                                                                                    |                                                                                                                               
aaataaatTACGAAAAGTTAAAGAAAGTACTAAATCCAAAAAGATTAAACGCTAAAAGATAATCAAAGCAACAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGa                                                                                                                          <  2:3192025/140‑1 (MQ=255)
  aTCAATTACTCAAAGTGAAATAAAGTACGAAATCCAAAAATATTCAACGATAAAACACAATAAAACAAACAGATCTGAAAAAGAGGCAGACAATAAAAAACAAACGAAAGAAAAATAAGATAAAAAAGAAGAAAGCGACa                                                                                                                        <  2:2040187/140‑1 (MQ=255)
  aTCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACa                                                                                                                        >  2:775919/1‑140 (MQ=255)
   tCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAg                                                                                                                       >  2:990375/1‑140 (MQ=255)
   tCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAg                                                                                                                       >  2:1752450/1‑140 (MQ=255)
      aTTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGACAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGa                                                                                                                    >  2:1227166/1‑140 (MQ=255)
       ttACGAAAAGTTAAAGCAAGTACTAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACAATAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGAt                                                                                                                   <  2:777210/140‑1 (MQ=255)
        tACTCAAATTTAAAGAAATTAAGAAATCCAAAAATATTCAACGATAAAAGAGAATAAAACAAACAGCTCTCAAAAAGAGGCAGACACTAAAAAACAAACGAACCAAAAAGAATATAAAAAACAACAAAGCGACAGTGATa                                                                                                                  <  2:301116/140‑1 (MQ=255)
        tACACAAATTTAAACAAAGTACTAAATCCAAAAATATTAAACGATAAAAGATAATCAAACAAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACGAAAGAAAACGAACATAAAAAAGAAGAAAGCGACAGTGATa                                                                                                                  <  2:407825/140‑1 (MQ=255)
          cGCAAAGTGAAAGAAAGTACGAAATCCAAAAAGATTCAACGCTAAAAGACAATAAAAGAAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATaaa                                                                                                                <  2:1436983/140‑1 (MQ=255)
               aGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAACCTGga                                                                                                           >  1:1588354/1‑140 (MQ=255)
                         attACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGcc                                                                                                 >  1:636123/3‑139 (MQ=255)
                         aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAATAAAGCTACAGTGATAAACTGGAGATTAAAGCa                                                                                                 >  1:134611/1‑140 (MQ=255)
                         aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGcc                                                                                                 >  1:2084860/1‑139 (MQ=255)
                         aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                 >  2:1749272/1‑140 (MQ=255)
                         aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                 >  1:1626462/1‑140 (MQ=255)
                         aGTACGAAATCCCAAAAGATTCAACGCGAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGAGACTAAAAAAGAAACGAAAGAAAAAGATGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                                 >  1:1392962/1‑140 (MQ=255)
                           taacaaaTCCCAAAACATAAAACGCAAAAATACAATAAACCAAACAGCTCTCAAAAACAGCCACACACAAAAAAACAAACCAAATAAAAACAACAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  1:1221699/136‑1 (MQ=255)
                           tACTAAATCCCAAAAGATACAACGCTAAAAGAGAATAAAACCAAAAGATCTTAAAAAGAGGAAGACAATAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  2:1458295/140‑1 (MQ=255)
                           tACTAAATCCAAAAATATTCAACGATAAAATACAATAAAAGCAACACCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAACGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  2:283752/140‑1 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  2:2546362/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATCAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  1:1678673/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAATCaaa                                                                                               >  2:1952062/1‑139 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  1:1787864/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  1:2125884/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  2:3032326/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  1:1267858/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               >  1:3231013/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGAAAc                                                                                               >  1:3141551/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTCGAGATTAAAGAAAc                                                                                               >  2:258144/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  2:2257709/140‑1 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACGAAAAAAGAACCGAAAGAAAAAGAAGATAAAAAAGACGAAAGCGCCAGTGATAAACTGGAGAGTAAATCAAc                                                                                               >  1:3085092/1‑140 (MQ=255)
                           tACGAAATCCCAAAAGATTCAACGATAAAAGATAATCAAAGAAACACCTCTTAAAAACAGCCAGACAATAAAAAATAAACGAAACAAAAACAACATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  2:17522/140‑1 (MQ=255)
                           tACCAAATCCAAAAACATAAAACGATAAAAGAGAAACAAACCAACAGCTCTTAAAAAGAGCCAGACAAAAAAAAACAAACGAAAGAAAAACAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                                               <  2:1100848/140‑1 (MQ=255)
                             ctaaaTCCCAAAAGATTAAACCCTAAAATATAATAAAAGCAACAGCTCTTAAAAAGAGGCATACACTAAAAAACAAACGAAACAAAAACACCATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                             <  2:914980/138‑1 (MQ=255)
                             ctaaaCCCCAAAACATACAACGCTAAACCACAATCAACCACACCCATCAGAAAAAGAGCCAGAAACTAAAACACAAACCAAAAAAAAAGACGATAAAAACCAACAAAGCGACAGTGATAACCTGGAGATTAAAGCAACTg                                                                                             <  1:3436139/138‑1 (MQ=255)
                             caaaaTCCCAAAACATTAACCTCTAAAAGACAAACAACCAAACAGCTGGGAAAAACAGGCCAACAAAAAAAAACAACCGAAACAAAAAGTAGATAAAAAAGACCACCGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                             <  1:2960479/138‑1 (MQ=255)
                             cGAAATCACAAAATATTAAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCATACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTg                                                                                             <  2:3430008/140‑1 (MQ=255)
                              gAAATCCCAAATGTTTCAACGCTAAATTAGAATCAACGCAACAGCTATGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGACGATAAAAAATCAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                                            <  2:1339694/140‑1 (MQ=255)
                                  tCCCAATAGCTTCAACGCTAAAACCGAATCAACGCAACAGCTCTGAAAAAGAGGCACACAATAAAAAAGAAACGAAAGAAAACGAAGATAAAAACGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                        <  2:3371824/140‑1 (MQ=255)
                                  tCCCAAAATATACAACGATAAAACACAATAAAAGAAACAGCTCTGAAAAAGAGCCATAAACTAAAAAATAAACGACACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                        <  2:344061/140‑1 (MQ=255)
                                  tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                        >  2:59144/1‑140 (MQ=255)
                                  tCCAAAAAGATACAACTCTAAAAGAGAATCAAAGCAACAGATCTGAAAAAGAGGCAGACAATAAAAAACAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                                        <  2:2458916/140‑1 (MQ=255)
                                    ccAAAATATACAACGATAAAACAGAATAAAACAAAAAGATCTGAAAAAGAGGAACACAATAAAAAAGAAACGAAACAAAAAGAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                      <  2:1065920/140‑1 (MQ=255)
                                    ccAAAAGATTCAACCATAAAACACAAACAACCCAACCGCTCTCAAAATGAGGCACAAACAAAAAAACAAACCAAACAAAAACACCAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                      <  1:1168150/140‑1 (MQ=255)
                                    caaaaaGATTCAACGATAAAATATAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAACTAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGa                                                                                      <  2:1076395/138‑1 (MQ=255)
                                     cAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                     >  1:3358965/1‑140 (MQ=255)
                                     aaaaaGTTTCAACGATAAACTATAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAACAAACCAAACAAAAATAAGATAAACAAGAACAAAGCGACAGTGATAAACTGGAGATTAGAGCAACTGGCACTGaa                                                                                     <  1:2570642/139‑1 (MQ=255)
                                     aaaaaGATTCAACGCTAAAAGAGAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                                     <  2:2942187/139‑1 (MQ=255)
                                        acgATTCACCGATAAAACACAAAAAACGCACCCGCTCTCAAAAATCGGCACACACTAAAAAAGAAACGAAACAAAACGAAGAAAAAAAAGACCACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                  <  1:785382/138‑1 (MQ=255)
                                        aacaTTCACCGCTAAAATATAATAAAACCAACCGCTCTCAAAAAGAGCCACACACTAAAAAACAAACCAAAAAAAAAGAACATAAAAACCAACACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                                  <  1:652115/137‑1 (MQ=255)
                                           aTTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCa                                                                               >  1:909152/1‑140 (MQ=255)
                                             tCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                                             >  2:1834874/1‑140 (MQ=255)
                                                actcTAAAATCGAAACACAGCCACAGCTCTTAAAAAGAGGCCTCAACCAAAAAAGAAACGACAGAAAAACAAGATAAAAACCAACAAAGCGACAGTGATAAACGGGAGATTAAAGCAACTGGCACTGAAGGGACATTAAc                                                                          <  2:2527472/137‑1 (MQ=255)
                                                actcTAAAAGACAACCAAACAAACCGGTATAAAAAAGACCCACAAACAAAAAAACAAACAAAACAAAACCAATAAAAAAACCAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  1:77677/137‑1 (MQ=255)
                                                actcTAAAACACAATCAAAGCAACAGCTCTGAAAAAGAGGCAGAAAATAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  2:3358965/137‑1 (MQ=255)
                                                acgacaaaaGACAATCAACCCAAACCCTCTCAAAAATAGCCACACACTAAAAAACAAACCAAAGAAAACCAAGATAAACAACAACACAGCGCCAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  1:2984699/135‑1 (MQ=255)
                                                aCGCTAAAAGATAATCAAAGCAACAGATCTTAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  2:517376/140‑1 (MQ=255)
                                                aCGCTAAAACAGAAACAAAGCAACAGCTATGAAAAAGAGGCAGACACTAAAAAATAAACTAACGAAAACGAAGATAAAAACTAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  2:3027928/140‑1 (MQ=255)
                                                aCGCTAAAACACAAAAAACGCAACAGGTCTTAAAAAGAGCCATACACTAAAAAACACACCAACTAAAAACAATAAAAAACATAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                                          <  1:1448855/140‑1 (MQ=255)
                                                aCGATAAAACAGAATAAACCCAACAGCTCTGAAAAAGAGGCACCCCCTAAAAAACACACGAACCAACACCACGAACAACAACAACAAACCGACAGTGATACACTGGAGATTAAAGCAACTGGCACTGAAGCGTCATTAAc                                                                          <  1:963502/140‑1 (MQ=255)
                                                     aaaaCACAATCAAACCAACACCTCTAAAAAAGCGCAACACAATAAAAAACAAACAAAACAAAAAGAACAAAAACAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACtt                                                                     <  1:2724879/140‑1 (MQ=255)
                                                        atacaaTAAAAGCAACAGCACTGAAAAAGAGGCAGACACTAAAAAAGAAACGAACCAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                  <  2:1787864/136‑1 (MQ=255)
                                                        agagAATAAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACCAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATg                                                                  <  2:760068/140‑1 (MQ=255)
                                                        acagaAACAACGCACCAGCTCTGAAAAATAGCCAGACACTAAAAAAGAAACGAAACAAAAACAAGATAAAACACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACGGAAGGGTCATTAACGACTTATg                                                                  <  2:1984473/138‑1 (MQ=255)
                                                          ataaacAAAGCACCCGATATCAAAAATAGCCACACCATAAAAAACAACCCAACCAAAAAGACGATAAAAAACAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                <  1:1260982/135‑1 (MQ=255)
                                                          agaCTCAAAGCCACCGCTCTGAAAAAGAGGCAGACACTAAAAATTAAAGCAAAGAAAAACAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                <  2:2125884/140‑1 (MQ=255)
                                                          agaATCAAAGCAACACCGCTGAACAAGACGCAGCCACTAACAAAGCACCGAAAGAAACCCAACATAAACACCCCCCCAGCGACAGTGATAAACAGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATgca                                                                <  1:844516/140‑3 (MQ=255)
                                                          agaACCAAACCAACCGCTCTCAAAAAGTGGCAGACACTAAAAAATAAACGAAAGAAAAAGACGATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                <  2:1350881/140‑1 (MQ=255)
                                                          acaacaaaaCCAACACCTCTGAAAATGAGGCCCACAATAAAAAACAAACCAAACAAAACCAACAAAAAAACGCCCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                                                <  1:1010306/134‑1 (MQ=255)
                                                            aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                              >  2:937714/1‑140 (MQ=255)
                                                            aaTCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgt                                                              >  2:771836/1‑140 (MQ=255)
                                                                  aGCAACATCTCTGAAAATGTGGCAGACACTAAAAAAGAAACGAAAGAAAAAGTAGATAAAAAAGAAGAAAGCGACAGTGATAAAATGGAGATTAAAGAAACTTTCACTGAAGGGTCATTAACGACTTATGAAGTGTCAgg                                                        >  1:1392098/1‑140 (MQ=255)
                                                                  aGCAACAGCTCTGAAAAATAGGCAGACACTAAAAAAGAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCgg                                                        >  2:1627485/1‑140 (MQ=255)
                                                                  aGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCgg                                                        >  1:766956/1‑140 (MQ=255)
                                                                       cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTg                                                   >  1:2252805/1‑140 (MQ=255)
                                                                              taaaaaGAGGCACACACTAAAAAAGAAACTAAATAAAAAGACGATAAAAAAGAAGACAGCGACAGTGATAAACTGGATATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAAtt                                            <  2:1161220/139‑1 (MQ=255)
                                                                               acaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAATGAAGAGAAAAAAGAAGAAAGCGACAGTGATACACGGGAGATTAAAGCAACTGGCACTGAAGGGTCTTTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                           >  1:57966/3‑140 (MQ=255)
                                                                               aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATGAACTGGAGATTAAAGCAACTGGCACTGCAGGGTCATTAACGACTTCTGAAGTCTCCGGCGCTGAGAAAGTc                                           >  2:1987653/1‑140 (MQ=255)
                                                                               aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                           >  2:505011/1‑140 (MQ=255)
                                                                               aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                                           >  2:826924/1‑140 (MQ=255)
                                                                                   agagGCATACAATAAAAAATAAACGACATAAAAAGACGATAAATAAGAAGAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACGGAAGGGTCATTAACGACTTATGAAGGGTCCGGCGCTGAGAAATTCGAGc                                       <  2:3172251/140‑1 (MQ=255)
                                                                                   agagGCATACAATAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                       <  2:667126/140‑1 (MQ=255)
                                                                                   agagGCAGACAATAAAAAAGAAACGAAAGAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGc                                       <  2:1573685/140‑1 (MQ=255)
                                                                                    gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTATAGCt                                      >  2:3160744/1‑140 (MQ=255)
                                                                                            acatAAAAAACAACCAAAACAAAAACACTATAAAAAATACACCAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa                              <  1:250890/137‑1 (MQ=255)
                                                                                               tAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAgg                           >  1:2515254/1‑140 (MQ=255)
                                                                                                    aacaaaCCAAACAAAAACAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCa                      <  1:133196/137‑1 (MQ=255)
                                                                                                      gAAACGAACTAAAAAGAAGATAAAAAATAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  1:1752450/140‑1 (MQ=255)
                                                                                                      caacccaaacaaaaagaaTATAAAAACCAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  1:3290893/134‑1 (MQ=255)
                                                                                                      caaaCGAAACAAAAACAACATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  2:1155006/139‑1 (MQ=255)
                                                                                                      caaaCCAAACAAAAACAACATAAAAACCACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  1:2999041/139‑1 (MQ=255)
                                                                                                      aaacccaaaaaaaaacaaCAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  1:990375/134‑1 (MQ=255)
                                                                                                      aaaaCGACATAAAAAGACCATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa                    <  2:2083683/139‑1 (MQ=255)
                                                                                                        aaCCAAACAAAAAAAACATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACa                  <  2:2252805/140‑1 (MQ=255)
                                                                                                         aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                 >  1:154404/1‑140 (MQ=255)
                                                                                                         aCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAg                 >  1:2913709/1‑140 (MQ=255)
                                                                                                                aaaaagaaGATAAAAAACAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTgg          <  2:3027582/140‑1 (MQ=255)
                                                                                                                aaaaacaaGCAAAAAAATACCAAAGCGACACTGATAAACTGGAGATTAAATCAACTGGAACTGAAGGGTCATTATCGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGTACTGAAGGCATCACAAAGTTCTTgg          <  2:1055807/140‑1 (MQ=255)
                                                                                                                        gaTAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGt  <  2:2603033/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                               
AAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAACAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGACAGTTCTTGGATTCGAGT  >  NZ_CP034943/1729964‑1730223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑