Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 452,125 T→A K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA98.2% 760.3 / ‑4.2 225K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/4);  new base A (82/139);  total (82/143)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261
                                                                                                                                          |                                                                                                                                        
tttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGtatt                                                                                                                                        <  2:50509/140‑1 (MQ=255)
     gCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGAt                                                                                                                                   <  1:764269/140‑1 (MQ=255)
        ttgTTTTTGATGATGGAAAGCTTTTTGAAGAAGAGTGGCCTTTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  1:2633764/138‑1 (MQ=255)
        tCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  1:621112/140‑1 (MQ=255)
        tCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  2:2705639/140‑1 (MQ=255)
        tCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  1:1186448/140‑1 (MQ=255)
        tCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAg                                                                                                                                <  2:2070531/140‑1 (MQ=255)
                gatgatGGAAAGCGTTTTGAAGAAGAGTGGCCTGACAAAATAAACCGACTGGTGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCaa                                                                                                                        <  2:2369290/140‑1 (MQ=255)
                     tgGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGtatt                                                                                                                                        >  2:1682151/1‑119 (MQ=255)
                        aaaGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAac                                                                                                                <  2:706197/140‑1 (MQ=255)
                          aGCTTTTTTAAGAAGAGTGGCCTGACAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  1:3213712/140‑1 (MQ=255)
                          aGCTTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  1:3317718/140‑1 (MQ=255)
                          aGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              >  1:671159/1‑140 (MQ=255)
                          aGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:175068/140‑1 (MQ=255)
                          aGCGTTTTGAACAAGAGTGGCCGGCCAAAAAAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGATGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCACacga                                                                                                              <  1:621791/140‑1 (MQ=255)
                          aGCGTTTTCAAGAAGAGTGGCCTCTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  1:2385339/140‑1 (MQ=255)
                             tttttGAACAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACgg                                                                                                           <  1:1809864/139‑1 (MQ=255)
                             gTTTTGAAGAAGAGTGGCCTGAAAAAATAAACCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACgg                                                                                                           <  1:1706668/140‑1 (MQ=255)
                                     gaagaGTGGCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATg                                                                                                   <  1:2761105/140‑1 (MQ=255)
                                     cacgagTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATg                                                                                                   <  1:3383181/137‑1 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                  >  1:167145/1‑140 (MQ=255)
                                      aagaGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGa                                                                                                  >  2:1730647/1‑140 (MQ=255)
                                           tGGCCTGTCAAAATAAACCGACTCGGGTTTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTTTTCCCTCAGTATTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                             <  1:3325990/140‑1 (MQ=255)
                                            ggCCTGGTCAACAAAAACCGACGCGGCTTTCGCAACGCCATGAACAGCATCAAATTGATAAATCGCCAAATTGGGATGCTGTTGTTCCCTCACTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                             <  1:3076405/140‑1 (MQ=255)
                                             gCCTTTAAAAATAACCCTACTCGGGATTGGCAAAGCCATGAAGACCTTCAAATTTATAAATCTCCAAATTGGGATGCTTTTGTTCCCTCATTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:1286823/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:2280538/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:2905482/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:2472426/140‑1 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCAGATGAAGGTaaa                                                                                           >  1:2108122/1‑140 (MQ=255)
                                             gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAAGTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  2:750814/140‑1 (MQ=255)
                                             gCCTGTCAAAAAAAACCGACTCGGGATTGGCAAAGCCATGACGAGCCTCACATTGATAACTCTCCAAATTGGGATGCTGTTGTACCCTCATTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:2001694/140‑1 (MQ=255)
                                             gCCTGACAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:418596/140‑1 (MQ=255)
                                              gcgtttaaaaaTAAACCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTTATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:897761/133‑1 (MQ=255)
                                              ccTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTTTTGTTCCCTCAGTATTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGaaaa                                                                                          <  1:2257744/140‑1 (MQ=255)
                                               cctttaaaaaTAACCCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaaa                                                                                          <  1:2074489/134‑1 (MQ=255)
                                                tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                        >  1:2689462/1‑140 (MQ=255)
                                                 ggTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCCAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                        >  2:631279/2‑140 (MQ=255)
                                                   taaaaaTAACCCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGCTGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      <  1:142608/138‑1 (MQ=255)
                                                   taaaaaTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAacga                                                                                                              <  2:1385041/114‑1 (MQ=255)
                                                   tCAATATAAACCTAATCGTGATTGGCAATGCTATGAAGAGCATAAATTTGATAAATCTCCAAATTGGGTTGCTGTTTTTCACTTAGTATTTTGATAATAACCTCAACATAAAACGACGGTTCATATTAAGGGaaaaaaaa                                                                                      <  2:953959/140‑1 (MQ=255)
                                                   tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  2:131093/1‑140 (MQ=255)
                                                   tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  1:2306997/1‑140 (MQ=255)
                                                   tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                      >  1:1582636/1‑140 (MQ=255)
                                                             cccaCTCGGTATTGGCACAGCCATGAAGAGCATCAATTTTATAAATCTCCAAATTGGGATGATGTTGTTCCGGCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            <  1:1832719/137‑1 (MQ=255)
                                                             ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            <  2:1574275/140‑1 (MQ=255)
                                                                 cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                        >  2:858742/1‑140 (MQ=255)
                                                                      gATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGc                                                                   <  1:2780027/140‑1 (MQ=255)
                                                                                gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACg                                                                >  1:1094556/1‑133 (MQ=255)
                                                                                gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                         <  2:3189594/140‑1 (MQ=255)
                                                                                gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCACAGTATTTTGATAAGAACCGCAACAACAAACGACGGTTCATATGAAGGGaaa                                                                                           <  1:3087668/106‑1 (MQ=255)
                                                                                   aTGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAagag                                                      <  1:131093/140‑1 (MQ=255)
                                                                                          tcATCAAATTTATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                               <  2:3044179/139‑1 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGACAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:2835916/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAGAACAATAGGACCGGGCAGAGTGGCACGTGATGAAGAGAATCGCGt                                              >  2:3354056/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:3271982/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:1473251/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:1251517/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                              >  1:865072/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATGTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGACAAACAATAGGACCGGGCAGACTGGCCCGTGATGAAGAGAATCGCGt                                              >  1:2884675/1‑140 (MQ=255)
                                                                                           cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGAAATTTGATAACAACCGCAACATCAAACGACGGTTCATTTGACGGGAAAATCAATCGGACCGGGGCGACTGGCAGGGGATGAGGGGGCTCGCGt                                              >  2:2282990/1‑140 (MQ=255)
                                                                                              cAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATc                                           >  1:2977328/1‑140 (MQ=255)
                                                                                                aaTTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAGAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCg                                         >  1:1241569/1‑140 (MQ=255)
                                                                                                  tttaTAAATCTCCAAATTGGGATGCTGTTTTTCCCTGACTATTTTGATAACAACCGCAACATCAAACCACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACCTGATGAAGAGAATCGCGTATCCggg                                       <  2:106178/137‑1 (MQ=255)
                                                                                                  ttGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                       <  2:905991/140‑1 (MQ=255)
                                                                                                   tGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGa                                      >  1:2593702/1‑140 (MQ=255)
                                                                                                         aTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGta                                <  2:2977328/140‑1 (MQ=255)
                                                                                                              cAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaac                           >  1:3333347/1‑140 (MQ=255)
                                                                                                              cAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaac                           >  1:2668842/1‑140 (MQ=255)
                                                                                                                    gggATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                            <  2:2223446/85‑1 (MQ=255)
                                                                                                                      gATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                   <  2:2991286/140‑1 (MQ=255)
                                                                                                                      gATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCt                   <  2:1149898/140‑1 (MQ=255)
                                                                                                                          cTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                 >  2:621717/1‑74 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATACGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTAACCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:1737172/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGACCCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCCCGTGATGCAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1500636/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAATAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1241569/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:2157164/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:394804/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  1:1453364/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:3090395/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:676817/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTCAGGATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:2532053/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTAAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGTCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:252642/140‑1 (MQ=255)
                                                                                                                           tgttgtTCCCTAAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1450721/140‑1 (MQ=255)
                                                                                                                           ggttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt              <  2:1110273/139‑1 (MQ=255)
                                                                                                                             ttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCa            >  1:1144246/1‑140 (MQ=255)
                                                                                                                             ttgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCa            >  2:1022390/1‑140 (MQ=255)
                                                                                                                              tgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  2:2689462/140‑1 (MQ=255)
                                                                                                                              tgttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  1:1022390/140‑1 (MQ=255)
                                                                                                                              tgttCCCTCAGTATTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  2:3434587/140‑1 (MQ=255)
                                                                                                                              tggtCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc           <  2:1870074/137‑1 (MQ=255)
                                                                                                                               gttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAcc          >  1:2533/1‑140 (MQ=255)
                                                                                                                                ttCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCt         <  2:2593702/140‑1 (MQ=255)
                                                                                                                                 tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc        <  2:960436/140‑1 (MQ=255)
                                                                                                                                  cccTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCa       >  1:1861720/1‑140 (MQ=255)
                                                                                                                                     tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAATAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:379382/1‑140 (MQ=255)
                                                                                                                                     tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:1630502/1‑140 (MQ=255)
                                                                                                                                     tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  1:921225/1‑140 (MQ=255)
                                                                                                                                     tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc    >  2:2104701/1‑140 (MQ=255)
                                                                                                                                       aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATaaca                          >  1:2628070/1‑116 (MQ=255)
                                                                                                                                       aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt  >  2:760384/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAT  >  NZ_CP034943/451988‑452261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: