New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 3471153416 (1.600)3 (0.010) 3/250 NT 0.72% coding (683/1905 nt) EO946_RS17905 glycosyltransferase
?NZ_CP034943 3471159 = 410 (1.590)coding (677/1905 nt) EO946_RS17905 glycosyltransferase
Rejected: Frequency below/above cutoff threshold.

AATGGCGATTTAAAAACTTCGCCATAAAATTCATTTTGCCGGTATGAGAAAGACGGCTCTCACAAATCATATCTATGATGACCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/3471070‑3471153
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  >  NZ_CP034943/3471159‑3471297
                                                                                                                                                                                                                               
AATGGCGATTTAAAAACTTAGACATAAAATTCATTTTGCCGGTATGAGAAAGACGGCTCTCACACATCATATCTATGATGACCTGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATG                                                                                     <  1:1614428/140‑1
                                                      GGCTCTCACAAATCATATCTATGATGACCTGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCA                                                                                       <  2:803443/84‑1
                                                      GGCTCTCACAAATCATATCTATGATGACCTGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCA                                                                                       >  1:803443/1‑84
                                                                                ACCTGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTC                                     <  1:4189604/108‑1
                                                                                ACCTGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTC                                     >  2:4189604/1‑108
                                                                                   TGCACCGTAGTCGCTGTGAGGCGCAACATAAGCAAATGTCACATGCTCGCCGTCATGCTGTACAGCGTAGTAAGAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  2:2578950/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGAGAGAAATTTTGTCCGGTTCTAAATAATCTTTCATGACAAATACCTGAT  <  1:2719792/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:2991812/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:305996/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:326181/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:4069480/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:4291742/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  2:407831/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  2:4238264/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  2:716722/140‑1
                                                                                   TGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  <  1:1124685/140‑1
                                                                                   TGCACCGTAGGAGCTTTGAGGCGCAACATAACCAAATGTCATATGCCCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAACTAAGCTTTCATGACAAATACCTGAT  <  1:3039800/140‑1
                                                                                   TGCACCGGAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATCAGCTTTCATGACAAATACCTGAT  <  1:2042528/140‑1
                                                                                                                                                                                                                               
AATGGCGATTTAAAAACTTCGCCATAAAATTCATTTTGCCGGTATGAGAAAGACGGCTCTCACAAATCATATCTATGATGACCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/3471070‑3471153
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tGCACCGTAGTAGCTTTGAGGCGCAACATAAGCAAATGTCATATGCTCGCCGTCATGCTGTACAGCGTAGTAATAATCATAGTCGGCCAAGACAGAAATTTTGTCCGCTTCTAAATAAGCTTTCATGACAAATACCTGAT  >  NZ_CP034943/3471159‑3471297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.