BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	132,185	G→A	5.6%	D560N (GAC→AAC)	fusA →	elongation factor G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	132,185	0	G	A	5.6%	1373.7 / 44.8	410	D560N (GAC→AAC)	fusA	elongation factor G
Reads supporting (aligned to +/- strand): ref base G (238/148); new base A (10/13); total (248/162)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 410 total aligned reads displayed.

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                                                       >  2:3395793/1‑140 (MQ=255)

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                                                       <  1:153992/140‑1 (MQ=255)

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACAt                                                                                                                                       <  2:4056993/140‑1 (MQ=255)

aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACAt                                                                                                                                       <  2:646239/140‑1 (MQ=255)

      gaagGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGc                                                                                                                                 >  2:3631648/1‑140 (MQ=255)

        agGCGCTGGCTTTGAATTTGATAATGCGATTGTCGGTGGGGTGGTTCCTCGTGAATACAGCCCAGCTTTTCAAGCAGGTCTGGAAGATGCACTCGAAAATGGTGTCCTAGCAGGCTTCCCTCTTATCGACATTAAAGcta                                                                                                                               <  2:1752353/140‑3 (MQ=255)

                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTTAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTTAAGCAAAACTTTTCg                                                                                                                      <  1:4035887/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                                                     <  2:2629093/140‑1 (MQ=255)

                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGa                                                                                                                     <  1:1086670/140‑1 (MQ=255)

                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                              <  1:2210935/140‑1 (MQ=255)

                             aaaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTAcc                                                                                                          <  1:903440/140‑1 (MQ=255)

                                tGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCatg                                                                                                       <  1:2871366/140‑1 (MQ=255)

                                             ggggTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCaaa                                                                                          <  2:3664330/140‑1 (MQ=255)

                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   >  1:3283314/1‑140 (MQ=255)

                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   >  1:3690807/1‑140 (MQ=255)

                                                    ttCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTGGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATg                                                                                   >  1:1773926/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:1641371/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:4143087/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:1048826/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  1:2348125/1‑140 (MQ=255)

                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                              >  2:2075149/1‑140 (MQ=255)

                                                              aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATTATGTTGACTCAAACGAAATGGCATTTAAAg                                                                         >  2:1865370/1‑140 (MQ=255)

                                                              aTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAg                                                                         >  2:2843000/1‑140 (MQ=255)

                                                               tACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGAt                                                                                                                    >  2:1969047/1‑96 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCaaa                                                                                          <  2:2249220/119‑1 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCaaa                                                                                          >  1:2249220/1‑119 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  2:2416697/140‑1 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgc                                                                     <  2:513995/140‑1 (MQ=255)

                                                                  aTCCCAGCTGTTCAAGCAGGTCTGGAAGAGGCACTTGAAAAGGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAACCGAAATGGCATTTAAAGTtgc                                                                     <  2:3921359/140‑1 (MQ=255)

                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:4003132/140‑1 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGCAATGGCATTTAAAGTtgctg                                                                   >  2:1593262/1‑140 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  2:3147412/1‑140 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  1:1434969/1‑140 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                   >  2:1630421/1‑140 (MQ=255)

                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  2:2574086/139‑1 (MQ=255)

                                                                     ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctgc                                                                  <  1:819044/140‑1 (MQ=255)

                                                                     ccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctgc                                                                  <  1:1294330/140‑1 (MQ=255)

                                                                      cAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAAAGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACGTTGCGATGGTTCTTACCATGATGATGACTCAAACGAAATGGCATTTAAAGTtgctgcg                                                                 >  2:916811/1‑139 (MQ=255)

                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                                                                >  2:2936355/1‑140 (MQ=255)

                                                                        gCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTc                                                               >  2:725548/1‑140 (MQ=255)

                                                                         cTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCt                                                              >  1:3631713/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:2089807/1‑140 (MQ=255)

                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTGTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGGTGCTTCTATg                                                           >  1:2801968/1‑140 (MQ=255)

                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                         >  2:155999/1‑140 (MQ=255)

                                                                               aaGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                        <  1:3509871/140‑1 (MQ=255)

                                                                               aaGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                        <  2:2918216/140‑1 (MQ=255)

                                                                                    ggTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaa                                                   >  2:2153682/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:3484938/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:235391/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  1:182918/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:1362588/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                                 >  2:437282/1‑140 (MQ=255)

                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTGTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAGTGacaa                                                 >  1:2510248/1‑140 (MQ=255)

                                                                                         ggAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                              <  1:3908643/140‑1 (MQ=255)

                                                                                           aataTGCACTTGAAAATGGTGTACTTGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGATTCTATGGCATTGAAAAATGCa                                            <  1:3061077/137‑1 (MQ=255)

                                                                                               tGCACTTGAAAATGGTTTAATGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTATTACCATGATGTTGAAAAAAACTAAATGGAATTTAAAGTTGATGGTAATATTGCATTGAAAAATGCAGACa                                        >  2:2280221/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCg                                                                                                                      <  1:2306862/62‑1 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGTTTCTTACCATGTTGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAGTGAAAAATGCAGTCa                                        >  1:3583621/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGTTTCTTACCATGATGTTGACTCAAACGAAATGTCATTTAAAGTTGCTGATTATATGGCATTTAAAAATGCAGTCa                                        >  1:70102/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1608935/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:487479/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:3229752/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:2539097/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:3846371/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1936872/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:574146/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  2:81630/1‑140 (MQ=255)

                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                        >  1:1022328/1‑140 (MQ=255)

                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  1:3730575/140‑1 (MQ=255)

                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCGTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:298699/140‑1 (MQ=255)

                                                                                                 cACTTGAAAATGGTGGACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  2:1919252/140‑1 (MQ=255)

                                                                                                    ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCACACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                   <  2:1131566/140‑1 (MQ=255)

                                                                                                    ttGAAAATGGTGTACTGGCAGGCTTCCCGATTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                   <  1:430025/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCTATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGGTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 >  2:3557917/1‑140 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:1724483/140‑1 (MQ=255)

                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                                 <  1:419939/140‑1 (MQ=255)

                                                                                                           tGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAcc                            <  2:848121/140‑1 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                           >  2:2717464/1‑106 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGTAGTCAGc                                      <  1:2589534/127‑1 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      >  2:3737109/1‑127 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                      <  1:3396878/127‑1 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  2:2500709/1‑140 (MQ=255)

                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                         >  1:1707566/1‑140 (MQ=255)

                                                                                                               gtACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                        <  2:1434969/140‑1 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       <  1:2067580/140‑1 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:3793324/1‑140 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       <  2:3277128/140‑1 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  1:241762/1‑140 (MQ=255)

                                                                                                                tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                       >  2:1093893/1‑140 (MQ=255)

                                                                                                                 acgggCAGGCGTCCCGCATAGCCACAATAAAGCAAAACGTTTCGATGGTTCTTACCAGGATGTTGACTCAAACGAAATGGCATTTAAAGGTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCTAATGTAACCCAGttc                      <  2:201928/137‑1 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                      <  2:1788683/101‑1 (MQ=255)

                                                                                                                        ggCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa               >  2:1185060/1‑140 (MQ=255)

                                                                                                                          cTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc             >  2:1257565/1‑140 (MQ=255)

                                                                                                                            tCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa           <  2:4119070/140‑1 (MQ=255)

                                                                                                                             cccGCTTATCGACATTAACGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCaa          <  1:2665020/140‑1 (MQ=255)

                                                                                                                                 cTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCAt      <  1:1216909/140‑1 (MQ=255)

                                                                                                                                   tATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAGTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa    >  1:3002303/1‑140 (MQ=255)

                                                                                                                                   tATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa    >  1:770165/1‑140 (MQ=255)

                                                                                                                                     tCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                    <  1:2925511/74‑1 (MQ=255)

                                                                                                                                     tCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGaaa  >  2:3085666/1‑140 (MQ=255)

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGAAA  >  NZ_CP034943/132050‑132322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`