Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,514 T→G 7.1% A119A (GCT→GCG EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5140TG 7.1% 482.9 / 3.3 203A119A (GCT→GCGEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base T (63/109);  new base G (13/8);  total (80/123)
Fisher's exact test for biased strand distribution p-value = 3.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607375‑607652
                                                                                                                                           |                                                                                                                                          
ccACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                            <  2:791840/140‑1 (MQ=255)
ccACGGGAGCGACCGGGGACGCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCt                                                                                                                                            <  2:1572494/140‑1 (MQ=255)
  cggggTGCTACGGGGGAAGCGGGACCAGCCGGACCAACGGGTGCGACAGTAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTgg                                                                                                                                          <  1:1639886/138‑1 (MQ=17)
   cGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGa                                                                                                                                         <  1:241569/140‑1 (MQ=255)
    gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                                                                                                                        >  2:656117/1‑140 (MQ=255)
    gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                                                                                                                        >  2:1694344/1‑140 (MQ=255)
    gggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                                                                                                                        >  2:2572005/1‑140 (MQ=255)
      gAGCTACCGGCGAACCCGGACCAGCCGGACCAACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCTGCTGGAGCg                                                                                                                                      <  2:1033397/140‑1 (MQ=255)
         cTACGGGGCAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                                   <  1:4189479/140‑1 (MQ=255)
         cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                                   <  1:4167794/140‑1 (MQ=255)
         cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                                   <  1:2760715/140‑1 (MQ=255)
         cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACg                                                                                                                                   >  2:3621720/1‑140 (MQ=255)
                 gcagccggagcaggcggaccAACGGGAGCGCCCCGCGCCCCCGGAGGTAAGGGGGCAGCCGGACCAGGGGGCGGGCCAGGAGCGACAGGAGCGACCGGAGCTACCGGGGTAGGTGGACCAGCTGGAGCGCCGGGAGCGAc                                                                                                                           <  2:1481151/138‑1 (MQ=255)
                 gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:1381686/140‑1 (MQ=255)
                 gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:4294869/140‑1 (MQ=255)
                 gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:959784/140‑1 (MQ=255)
                   acccggaccacccggactcCCGGGAGCCACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAg                                                                                                                         <  1:3340021/138‑1 (MQ=14)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCGGGAGCGACGGGAGCGCCAgg                                                                                                                        >  1:2996175/1‑140 (MQ=17)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                        >  2:2207090/1‑140 (MQ=255)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGAGAGGAGCTACCGGTGAAGCTGGACAAGCTGGAGCGACGGGAGCGACAgg                                                                                                                        >  1:58209/1‑140 (MQ=255)
                    gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCGGGAGCAGCGGGAGCGACTGGAGCGACAgg                                                                                                                        >  1:3061048/1‑140 (MQ=14)
                    gccgcaccagccggtccaCCCGGCGCGACCGGAGCGACCGGAGCGAGCGGGGAAGCCGGCCCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                                                                                                                        <  2:1515105/140‑1 (MQ=14)
                      cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                      <  1:2814967/140‑1 (MQ=255)
                         accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCCACAGGGGCGAGCGCTGCTGCCGGGGACTCTGTACCAGCGGGTGCGGCGGGGGCGACCggtgggc                                                                                                                   >  2:1822230/1‑135 (MQ=9)
                         accagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCGACGGGAGCCACAGGCGCGACCGGAGCTACCGGGGAAGCGGGACCCGCGGGAGGGCCGGGAGCGACCGGGGCtc                                                                                                                   >  2:577323/1‑139 (MQ=14)
                              ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                                                                                                                        <  1:2692407/114‑1 (MQ=255)
                                  accccccGGAGCGACAGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg                                                                                                          <  2:3878538/135‑1 (MQ=255)
                                   ccaCCCGGAGCGACTGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  1:3968719/140‑1 (MQ=255)
                                   ccaCCCGGAGCGACAGGCCCGCCAGGCGCGACCGGCGAAGCCGGACCAGCCGGCGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGATGGACCTGCTTGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  1:958537/140‑1 (MQ=18)
                                   ccaCCCGGAGCGACACGCGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  2:2380468/140‑1 (MQ=17)
                                     aaCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCTACAGGCGCTAACGGGGAAGCTGGACCAGCAGGAGCGACGGGCGCGCCAGGCGCTACCGGGGAAGCCg                                                                                                       >  1:3837271/1‑140 (MQ=18)
                                       ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGTGCGACAGGAGAGGCCGGAGCACCCGGAGCAGCTGGAGCATAAGGATCTACGGGAGCGACAGGCGCTACAGCGGCAGCAGGa                                                                                                     >  1:1546002/1‑140 (MQ=12)
                                        cGGCGCGACAGGTGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  1:3152286/140‑1 (MQ=255)
                                        cGGAGCCACCGCAGCGACCGGAGCTCCCGGGGAAGCCGCACCAGCCGGAGCCACAGGCGCGCCTGGAGCGACAGGAGCTACCGGGTAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  1:1511467/140‑1 (MQ=17)
                                        agtagggacaggagccacagtctcTTCGGGTGAATCCGGACCAGCCGGACCGACTGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  2:3787715/134‑1 (MQ=14)
                                           agcgacaggagcgacaggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              >  1:4013454/1‑127 (MQ=255)
                                           agcgacaggagcgacaggAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              <  2:4013454/127‑1 (MQ=255)
                                             cgagcggagccaccggagcTACCGGGGAAGCCGGACCACCCGGAGCGCCAGGACCGACAGGAGCGACAGGAGCCACCGGTGAAGCGGGACCACCCGGAGCGAAGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                               <  2:1998687/135‑1 (MQ=14)
                                                      ccacaggcgcTACCGGGGATGCCGGACCCTCCGGTGCGAGAGGAGAGTGAGGACCGACAGCCGCACCAGGCCAAGCTGGACCACCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                      <  1:2610212/138‑1 (MQ=12)
                                                        acgggagcTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  2:1047944/137‑1 (MQ=14)
                                                        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:2473916/140‑1 (MQ=255)
                                                        acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:3621720/140‑1 (MQ=255)
                                                         caggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                      >  2:3262109/1‑105 (MQ=255)
                                                          aggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                  <  1:3728728/140‑1 (MQ=255)
                                                               cgaCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:1927047/138‑1 (MQ=255)
                                                               ccaCCGGGGAAGCCGGACCAGCCTGAGAGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCTGGACCACCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  2:3984532/138‑1 (MQ=18)
                                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:550715/140‑1 (MQ=255)
                                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:1662146/140‑1 (MQ=255)
                                                               cTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:1731233/140‑1 (MQ=255)
                                                                 tggggggAAGCCGGAGCAGCGGGAGCTACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:2589057/137‑1 (MQ=255)
                                                                 aCCGGGGCAGCCGGACCTGCCGGCGCGACAGGAGCGACCGGAGCTACAGGCGCTCCCGGCGCAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:1291356/140‑1 (MQ=18)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         >  1:2368725/1‑110 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         >  1:1023104/1‑110 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  2:2368725/110‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                         <  2:1023104/110‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           >  1:855357/1‑140 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:245723/140‑1 (MQ=255)
                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCGGGAGCCACCGGAGCGACACGAGCTCCCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           >  2:2489796/1‑140 (MQ=21)
                                                                  ccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGAACCCGGAGCTACCGGGGCAGCGGGAGCGACGGGGGCTACGGGAGCGACCGGATCTACCGGGGACGCCGGCCCAGCCGGTGACCCGGGGGCGACAggg                                                                          >  2:1681992/1‑140 (MQ=12)
                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                         >  2:2837647/1‑140 (MQ=255)
                                                                    ggggAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGCGCAGCCGGAGAAGCTGGAGCAGCAGGAGCGACGGGAGCCACCGGAGCACCCGGTGCCGCAGGAGCACCCGGGGCCCCCGGAGCAACAGGAGc                                                                        >  1:3636241/1‑140 (MQ=9)
                                                                     gggAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCt                                                                       <  1:2653751/140‑1 (MQ=255)
                                                                     gggAAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCt                                                                       <  1:3989422/140‑1 (MQ=255)
                                                                      ggAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:484466/140‑1 (MQ=255)
                                                                      ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:1692467/140‑1 (MQ=255)
                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTAc                                                                     >  1:319990/1‑140 (MQ=255)
                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTAc                                                                     >  1:321148/1‑140 (MQ=255)
                                                                        aaGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 >  1:1712853/1‑79 (MQ=255)
                                                                           ccGGACCCGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                 <  1:2972587/140‑1 (MQ=255)
                                                                            cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACAGGGGAAGCTGTAACACAGGGACCGACGGGAGCGACAGCAGATACCTGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGTGCGACTgggg                                                                >  1:2094788/1‑140 (MQ=21)
                                                                               aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCACCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGGCGGACCAGCCGGGGCCACGGGGGCGACGGGGGCTACAggggcgg                                                             >  2:1714721/1‑137 (MQ=18)
                                                                                    ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:3803105/112‑1 (MQ=255)
                                                                                         gggagaggagcgacaggagcgactggttcTTCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                   <  1:2570005/135‑1 (MQ=21)
                                                                                                  gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                          <  1:656117/140‑1 (MQ=255)
                                                                                                  gcccctggagctccaggagcTCCCGGCCCAGCTGGCCCCCCTGGAGCGACGGGAGCGACAGGACCTACCGGCGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                          <  2:583454/134‑1 (MQ=18)
                                                                                                    gacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAg                                        <  2:4291009/140‑1 (MQ=255)
                                                                                                    gacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAg                                        <  2:319990/140‑1 (MQ=255)
                                                                                                    gacaggaccgaccggagcGACCGGCGAAGCCGGACCCGCTGGAGCGACGGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAg                                        <  2:1254877/140‑1 (MQ=21)
                                                                                                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                                                <  1:3494703/99‑1 (MQ=255)
                                                                                                     acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGcc                                                                                >  2:3494703/1‑99 (MQ=255)
                                                                                                          agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCGACAGGGGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTa                                  >  2:4243279/1‑140 (MQ=255)
                                                                                                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      >  2:2980340/1‑100 (MQ=255)
                                                                                                              acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACCGGAGCTCCCgg                              >  1:3864033/1‑140 (MQ=255)
                                                                                                                      tACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg                                                                                                              <  1:891629/52‑1 (MQ=35)
                                                                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTTCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                     <  1:3089112/140‑1 (MQ=255)
                                                                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                     >  2:3328175/1‑140 (MQ=255)
                                                                                                                       aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCgg                     >  1:1917242/1‑140 (MQ=255)
                                                                                                                         cgcggAAGCTGGACCAGCTGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                   <  2:3811241/137‑1 (MQ=255)
                                                                                                                             gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                     <  1:2905369/54‑1 (MQ=35)
                                                                                                                             gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                     >  2:2905369/1‑54 (MQ=35)
                                                                                                                             gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGc               >  2:2069767/1‑140 (MQ=255)
                                                                                                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg            >  1:2048927/1‑140 (MQ=255)
                                                                                                                                gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg            <  2:112022/140‑1 (MQ=255)
                                                                                                                                  tGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg            <  2:1640923/138‑1 (MQ=255)
                                                                                                                                  cggACCACCTGGCGCCCCGGGAGCCACAGGAGCTACCGGTGAAGCCGGACCAGCCGGTGCCACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAg          <  2:515074/139‑1 (MQ=14)
                                                                                                                                   ggACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTggg                                                                 >  2:2683735/1‑84 (MQ=255)
                                                                                                                                      ccAGCTGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCGGGACCAGCCGGGGCCACGGGAGCCACCGGGGTTACTGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc      <  1:750524/140‑1 (MQ=17)
                                                                                                                                      ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc      <  1:3269090/140‑1 (MQ=255)
                                                                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCAGGACCAGCCGGAGCGCCCGGa  >  2:2290430/1‑140 (MQ=17)
                                                                                                                                          cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACAGCGGAAGCCGGAACAGACGGAGCGAAAGGAGATACCGTGTAAGCCGAAACagcagcagccgaagga  >  1:2093131/1‑133 (MQ=17)
                                                                                                                                           |                                                                                                                                          
CCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607375‑607652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: