Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,682 G→C 5.3% A175A (GCG→GCC EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6820GC 5.3% 395.5 / 4.1 158A175A (GCG→GCCEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (89/56);  new base C (2/7);  total (93/65)
Fisher's exact test for biased strand distribution p-value = 3.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607544‑607818
                                                                                                                                          |                                                                                                                                        
ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACTGGAGCTACAGGGGAAGCCGGGGCAAACGGAGCgg                                                                                                                                         >  2:2026346/1‑139 (MQ=18)
ggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGa                                                                                                                                         <  2:1917242/140‑1 (MQ=255)
 gacgCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGAc                                                                                                                                        >  2:2950997/4‑140 (MQ=255)
 gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                        <  1:2837647/140‑1 (MQ=255)
 cccgCCGGCCCCGCCGGCGCCACCGGAGCCTCAGGCGCACCCGGCCCAGCCGGACCAGCCGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                        <  2:901721/137‑1 (MQ=11)
  cacccGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACCCGGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                                                                       <  1:1271970/137‑1 (MQ=17)
    gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                     <  2:652482/140‑1 (MQ=255)
    gCCGGACCAGCCGGCGCGACGGGAGCCACTGGCGCTACCGGGGAAGCTGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                                     <  2:756862/140‑1 (MQ=14)
      cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                                   <  1:1090176/140‑1 (MQ=255)
      cGGACCAGCCGGGGCCACGGGAGCAACAGGGGATACTGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGGGGCCGGAg                                                                                                                                   >  1:1139659/1‑140 (MQ=17)
              ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                           >  1:2520675/1‑140 (MQ=255)
              ccGGCGCCACGGGAGCCACAGCGGCGACGGGGCAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                                           <  2:3306672/140‑1 (MQ=14)
                  cgCCACGGGAGCCACCGGCGCATCTGGCCCAGCCGGACCCCCCGGAGCCCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                       <  2:2701482/139‑1 (MQ=14)
                   gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCCACCtgggccgc                                                                                                                      >  1:3679351/1‑136 (MQ=255)
                       cGGGAGCCCCAGGGGCTACGGGGGCCGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCAGCCGGAGCGACAGGAGCGAGCGGGGGAGCCGGCCCAAGCGGAGCTAGCGGAGCGTCAGTGGAGGCCGGa                                                                                                                  >  1:980631/1‑140 (MQ=9)
                        gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCAACGGGGGACGCAGGCGCACGCGGAGCGCCCGGAGCGCCCGGAGAGGCCggcc                                                                                                                 >  2:1134989/1‑138 (MQ=11)
                            gCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGACTCAGGACCACCCGGAGCGACAGTAGCGCCCTGAGACGCAGGACCacc                                                                                                             >  2:3599407/1‑138 (MQ=14)
                             ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGCAGCCGGCCCAccc                                                                                                            >  2:35084/1‑140 (MQ=14)
                             ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGGGAAGCAGGACCAACCGGAGCGCCCGTAGCTAAAGGCGAATCCCGACCAGcc                                                                                                            >  2:4062676/1‑140 (MQ=14)
                               acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACGGGAGCCCCCGGACCAGGCgg                                                                                                          >  1:726985/1‑140 (MQ=14)
                                    ggCTACTGGAGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                     <  1:1548264/140‑1 (MQ=255)
                                      caacggcggAAGCCGGACCACCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAGCCGGAGCGTCAGGCGCTACCGTGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACa                                                                                                   <  2:2188624/133‑1 (MQ=11)
                                       tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAATCCGGAGCAGCCGGAGCTACAg                                                                                                  >  2:3127439/1‑140 (MQ=255)
                                        aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACTCCGGACCAACCGGAGCGACAGTAGCTCACGGGGAAGCCGGACCAGCCGGAGCGGCAgg                                                                                                 >  1:1450704/1‑140 (MQ=14)
                                        aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                 >  2:355071/1‑140 (MQ=255)
                                          tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACAGGAGCTCCCGGGGAAGCCGCACCAGCCGGAGCGACAGGAg                                                                                               >  2:3908833/1‑140 (MQ=255)
                                          cggcgAACCCGGACCCGCCGGAGCGACAGGACCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                               <  1:1436881/136‑1 (MQ=11)
                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGTAGCGACCGGGGGAGCCGGACCAACCGGAGCGACAGGAGAAACCGGCGAACCCGGCCCAGCCGCAGCGACCGGAGCTACCgg                                                                                        >  1:643843/1‑140 (MQ=14)
                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGCAGCGTCGGGATCCCCCGGGGCAGCCGGAGCAGCCGGCGCGACAGGAGcgaactg                                                                                        >  2:616451/1‑134 (MQ=11)
                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTCCCgg                                                                                        >  2:1734228/1‑140 (MQ=255)
                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                        >  2:3178306/1‑140 (MQ=255)
                                                  ccGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                       <  1:2489796/140‑1 (MQ=255)
                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCGACCGGAGAAGCCGGACCAGCCGGAGCTGCCGGAGCGACAggcg                                                                                      >  2:1706702/1‑138 (MQ=11)
                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                   >  2:3527486/1‑140 (MQ=255)
                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCCTCCGGACCAGCCGGAGCGCGACGAGCGACCggggccg                                                                                   >  2:3782924/1‑137 (MQ=14)
                                                      aCCAGCCCGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                   <  1:1317356/140‑1 (MQ=255)
                                                                gccacgggAGCTACCGGGGAAGCCGGACCATCGGGAGCGACAGGAGCTACCGGTGAAGCCGGAGCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                         <  1:860032/134‑1 (MQ=14)
                                                                gccaCAGGAGCTACCGGCGCACCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                         <  1:2770137/137‑1 (MQ=14)
                                                                    cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGa                                                                     >  2:1119729/1‑140 (MQ=255)
                                                                    cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGa                                                                     >  2:2854579/1‑140 (MQ=255)
                                                                     aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCGACCGGCGCAGCCGGACCAGCCGGAGCCGCAGCAGCGACCGGCGAAGCAGGACAAACCGGac                                                                    >  2:2474254/1‑139 (MQ=14)
                                                                     aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGACCCGCCGGAGCGTCAGGAGCTACCGGGGCAGCCGGCGCACCCGGAg                                                                    >  2:3892528/1‑140 (MQ=14)
                                                                           tcccGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                              <  1:1121689/138‑1 (MQ=255)
                                                                            cccGGGGACCCCGGACCTGCCCGTGCCACAGGTCCGCCCGTGGACGACGGCCCAACCGGAGAGCCAGGACCTTCCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  1:4221198/139‑1 (MQ=9)
                                                                            cccGGGGAAGCCGGACCACCCGGAGCGCCCGGAGCTTCCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  1:1023660/139‑1 (MQ=14)
                                                                            cccGGGGAACCCGGACCCCCCGGAGCGACAGGAGCTCCCGGCGAACCCGGCCCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  1:4336493/139‑1 (MQ=14)
                                                                            cccGGCGCAGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  1:577323/139‑1 (MQ=14)
                                                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                             <  2:3864033/140‑1 (MQ=255)
                                                                             ccGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGCAGCACCCGGAGCACCCGGAGCAGCGGGa                                                            >  2:1457704/1‑140 (MQ=11)
                                                                                gggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                         <  1:355071/140‑1 (MQ=40)
                                                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                       <  1:3163841/140‑1 (MQ=255)
                                                                                  gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGTCACACGGACCAGCCGCAGCGACAGGAGCGCCCGGGGAAGCCGGCCCGAGCGGAGCGACGGGGGccgc                                                       >  1:1831145/1‑138 (MQ=14)
                                                                                   ccgCCGGACCCCCCGGACCGACCGCCGCTACCGGCGCCCCCGGACCCACCGGAGCCACAGGCGCTCCTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCcaca                                                      <  1:204084/138‑1 (MQ=11)
                                                                                   acccgggACCCCCCGGATCGACTGGAGCTACCGGTGAAGCCGTAGCAACCGGTGGTACTGGCGCTACCGGGGAAGCCGGACCAGCCGGTGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                      <  1:765480/135‑1 (MQ=14)
                                                                                     gCCGGACCCGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGCCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:1055636/140‑1 (MQ=255)
                                                                                     gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    >  2:1123832/1‑140 (MQ=255)
                                                                                     gCCGGACCACCCGGCGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  1:1681992/140‑1 (MQ=255)
                                                                                     ccaggACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  2:2020751/137‑1 (MQ=255)
                                                                                          aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCtc                                               >  1:1787367/1‑139 (MQ=255)
                                                                                          aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCGCCAGGGGCtc                                               >  1:266004/1‑139 (MQ=255)
                                                                                          aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGAAAGGGGCAGCGGGAGAAAACGTAGCGGCGGCAGCGACAGGTGCTa                                               >  1:3453784/1‑140 (MQ=14)
                                                                                            ctgCCGGAGCGACAGGAGCGACCGGCGAAGCAGGACCAACCGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt                                             <  2:2935476/138‑1 (MQ=17)
                                                                                               ccGGAGCTACCGGCGCTCCCGGCCCAGCCGGCGCTACCGGAGCCACTGGAGCTACGGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                          <  1:2132511/140‑1 (MQ=14)
                                                                                               ccGGAGCTACAGGAGATCCCGGCGAAGCCGGTCCAACCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                          <  1:2026346/140‑1 (MQ=17)
                                                                                               ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGCCGCCGGACATCCCGCAGCGGCGCGAGCCACAGGGGCTCCTggg                                          >  1:2206286/1‑140 (MQ=14)
                                                                                               ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                          <  1:1123832/140‑1 (MQ=255)
                                                                                                   agagACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                      <  1:3341966/137‑1 (MQ=255)
                                                                                                   aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGAGACGCCGGAACAGCCGCAGCGACAGAAGCTACAGTGGAAGCCGGACCAACAGGAGAGACGGAATCCACAGGGGCTACTggggcag                                      >  2:1732347/1‑137 (MQ=17)
                                                                                                         aGGCGCCACCGGGGCAGCGGGACCACCCGGAGCGACAGGCGCTCCCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                <  2:2048927/140‑1 (MQ=17)
                                                                                                         aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                <  2:460821/140‑1 (MQ=255)
                                                                                                              cTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACGGGCGAAGCCGGACCCGCCGCAGCGTCAGGAGCGACCGGGGCAGCCGGCCCACCCGCAGCGCCCGGAGCCACTGTGGCAACAGTCCACGTCGCACCAGcc                           >  1:4067235/1‑140 (MQ=9)
                                                                                                                aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                         >  2:167932/1‑140 (MQ=255)
                                                                                                                aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                         <  1:3443703/140‑1 (MQ=255)
                                                                                                                 ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCTACCGGGGATGCCGGACCAACCGGAGCGACGGGCGCCACAGGGGCTACAGGGGGCGTCGCGCCCGCCggt                        >  1:2077550/1‑139 (MQ=17)
                                                                                                                  cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGCGCCGCCGGACAAACCGGAGCTCGGGGAGCCACAGGCGCGACTCGCGACCAAGGACCCGCCGGAg                       >  1:3907270/1‑140 (MQ=9)
                                                                                                                      gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                   <  1:1870302/140‑1 (MQ=255)
                                                                                                                      gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                   >  1:1102511/1‑140 (MQ=255)
                                                                                                                         gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACCCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACCgg                >  1:3790729/1‑140 (MQ=255)
                                                                                                                         gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                >  2:3147522/1‑140 (MQ=255)
                                                                                                                         gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                >  2:3375248/1‑140 (MQ=255)
                                                                                                                         gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACGGGGGACGTCGGCCCAGCCGGAGCGACAgg                >  2:3326604/1‑140 (MQ=255)
                                                                                                                          ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCGACAGGCGCAACCGGACCAACCGGAGCGCCGGGAGCGACAGGGGGGACGGGGGACGCCGTACCAGCGGGATCGTCAGGa               >  2:2703742/1‑140 (MQ=14)
                                                                                                                          ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGCGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGa               >  2:2458643/1‑140 (MQ=255)
                                                                                                                           cGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGaa              >  2:4333905/1‑139 (MQ=255)
                                                                                                                              ggccacgggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa           >  2:1403214/8‑140 (MQ=18)
                                                                                                                              aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                       >  1:62149/1‑96 (MQ=40)
                                                                                                                                 aaCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCGACTGGGGACGACGGACCAGCCGGAGCGCCAGGAGCGACCg        >  2:1790662/1‑140 (MQ=18)
                                                                                                                                 aaCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGTGGACGTAGGACCAGCAGGAGCGACAGGAGCTACCg        >  2:2414588/1‑140 (MQ=21)
                                                                                                                                  aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAATCCGGACCAACCGGAGCGTCGGGAGCCACAGGCGCTACAGGGGAAGTCGGACCCGCAGCAGCGACAGGCGCTACCgg       >  2:2365529/1‑140 (MQ=18)
                                                                                                                                  aCCGGAGCACCAGGAGCTCCCGGCGACCCGGGAGCACCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg       <  1:3516480/140‑1 (MQ=17)
                                                                                                                                   cgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                 >  2:776649/3‑113 (MQ=255)
                                                                                                                                   ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTAGGACCAGCCGGAGCGACAGGAGCTACAggg      >  2:1835574/1‑140 (MQ=255)
                                                                                                                                   ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGTACGTCGGACCTGCCGGAGCGCCAGCAGCTACAggg      >  2:2244002/1‑140 (MQ=18)
                                                                                                                                   ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg      >  2:3943570/1‑140 (MQ=255)
                                                                                                                                   ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg      >  2:1725058/1‑140 (MQ=255)
                                                                                                                                       aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACCTGGGAACCCGGACCACCCGTAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAg  >  1:102637/1‑140 (MQ=21)
                                                                                                                                        gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                      >  2:610670/1‑87 (MQ=39)
                                                                                                                                        gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                      <  1:610670/87‑1 (MQ=39)
                                                                                                                                          gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    <  2:2381249/87‑1 (MQ=255)
                                                                                                                                          gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                    >  1:2381249/1‑87 (MQ=255)
                                                                                                                                          |                                                                                                                                        
GGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAG  >  NZ_CP034943/607544‑607818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: