BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,700	A→C	19.4%	E181D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,700	0	A	C	19.4%	336.5 / 78.4	210	E181D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (104/62); new base C (38/4); total (144/66)
Fisher's exact test for biased strand distribution p-value = 3.45e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01

Only 100 of 212 total aligned reads displayed.

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607563‑607838

     gggAGCCACAGGCGCGACTGGGGAAGCCGGACCAGCGGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                                                     <  2:3674563/140‑1 (MQ=255)

          ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGCAGCCGGCCCAccc                                                                                                                                >  2:35084/1‑140 (MQ=14)

             caGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGCCCAACCGGAGCGCCAGGAGCGCCCGGAGCAGCCGGCGCAGCCGGa                                                                                                                             >  2:4266174/1‑140 (MQ=14)

                 ggCTACTGGAGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                         <  1:1548264/140‑1 (MQ=255)

                  gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                        <  1:2207090/140‑1 (MQ=40)

                    tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAATCCGGAGCAGCCGGAGCTACAg                                                                                                                      >  2:3127439/1‑140 (MQ=255)

                     aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACTCCGGACCAACCGGAGCGACAGTAGCTCACGGGGAAGCCGGACCAGCCGGAGCGGCAgg                                                                                                                     >  1:1450704/1‑140 (MQ=14)

                       tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGACCGACAGGAGCTCCCGGGGAAGCCGCACCAGCCGGAGCGACAGGAg                                                                                                                   >  2:3908833/1‑140 (MQ=255)

                          ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACCGGAGCTACCGGGTAAGCCGGAGCAACCGGTGCGACAGGAGCCACCGGGGCAGGCGTAGAACCCGGAGCGACCGgcgcca                                                                                                                >  2:1752970/1‑138 (MQ=11)

                           gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGACGCCGGAGCAGCCGGGGAGCCAGGAGCTACGGGGGCAGCCGGAGCGACCGGAGCGACAGGAGCAACCGGTGAAGCCGGACCAGCCGGAGCGACCGGCGCTag                                                                                                               >  1:751824/1‑139 (MQ=14)

                            aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCTACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGCAGCGACCGGGGACGCCGGACCACCCGGCGCGACCGGAGCGAcc                                                                                                              >  2:2404114/1‑140 (MQ=11)

                              gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                            >  2:3178306/1‑140 (MQ=255)

                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGGCAGGATCGGCCgggg                                                                                                          >  2:853971/1‑140 (MQ=14)

                                cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCGACCGGAGAAGCCGGACCAGCCGGAGCTGCCGGAGCGACAggcg                                                                                                          >  2:1706702/1‑138 (MQ=11)

                                   aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGACGCCGGACCAACCGGAGCGACAGGAGCCACCGGCGCCTCCGGACCAGCCGGAGCGCGACGAGCGACCggggccg                                                                                                       >  2:3782924/1‑137 (MQ=14)

                                            aGCGACAGGAGCTCCCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACAGGAGCGACAGGAGCGCCCGTCGCCGCCGGACCGGCCGGAGCGCGGGGAGCGACCGGGGAAGCGGGGccca                                                                                              >  1:3987865/1‑138 (MQ=14)

                                            aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaa                                                                                              >  1:1358247/1‑140 (MQ=255)

                                             gccacgggAGCTACCGGGGAAGCCGGACCATCGGGAGCGACAGGAGCTACCGGTGAAGCCGGAGCAACCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                             <  1:860032/134‑1 (MQ=14)

                                             gccaCAGGAGCTACCGGCGCACCCGGACCAGCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                             <  1:2770137/137‑1 (MQ=14)

                                                 cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGa                                                                                         >  2:1119729/1‑140 (MQ=255)

                                                  aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGACGCCGGACCCGCCGGAGCGTCAGGAGCTACCGGGGCAGCCGGCGCACCCGGAg                                                                                        >  2:3892528/1‑140 (MQ=14)

                                                        tcccGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                                                  <  1:1121689/138‑1 (MQ=255)

                                                        tcccGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACgg                                                                                  <  1:4070102/138‑1 (MQ=255)

                                                         cccGGGGCAGCCGGCCCAGCCGGAGCGACAGGCGCTACCGGGGACGCCGGACCAACCGGTGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  1:1822230/139‑1 (MQ=14)

                                                         cccGGGGAAGCCGGACCACCCGGAGCGCCCGGAGCTTCCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  1:1023660/139‑1 (MQ=14)

                                                         cccGGGGAACCCGGACCCCCCGGAGCGACAGGAGCTCCCGGCGAACCCGGCCCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  1:4336493/139‑1 (MQ=14)

                                                         cccGGCGCAGCCGGCCCACCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                                                                 <  1:577323/139‑1 (MQ=14)

                                                             gggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                             <  1:355071/140‑1 (MQ=40)

                                                               gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  1:2609018/140‑1 (MQ=255)

                                                                ccgCCGGACCCCCCGGACCGACCGCCGCTACCGGCGCCCCCGGACCCACCGGAGCCACAGGCGCTCCTGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCcaca                                                                          <  1:204084/138‑1 (MQ=11)

                                                                  gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        >  2:1123998/1‑140 (MQ=255)

                                                                  gCCGGACCACCCGGCGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        <  1:1681992/140‑1 (MQ=255)

                                                                  ccaggACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        <  2:2020751/137‑1 (MQ=255)

                                                                         ctgCCGGAGCGACAGGAGCGACCGGCGAAGCAGGACCAACCGGAGCGACAGGAGCTCCCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACt                                                                 <  2:2935476/138‑1 (MQ=17)

                                                                            ccGGAGCTACCGGCGCTCCCGGCCCAGCCGGCGCTACCGGAGCCACTGGAGCTACGGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                              <  1:2132511/140‑1 (MQ=14)

                                                                            ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                                                              <  1:1123998/140‑1 (MQ=255)

                                                                               gAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACAAACCGGAGCGACGGGAGCCACAGGGGCAACTGGGGAc                                                           >  2:3530405/1‑140 (MQ=255)

                                                                                agagACAGGCGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                          <  1:3341966/137‑1 (MQ=255)

                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACg                                                          >  1:1283299/1‑140 (MQ=255)

                                                                                aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGAGACGCCGGAACAGCCGCAGCGACAGAAGCTACAGTGGAAGCCGGACCAACAGGAGAGACGGAATCCACAGGGGCTACTggggcag                                                          >  2:1732347/1‑137 (MQ=17)

                                                                                     cagcagCTACCGGCGACCCCGGACCAACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                     <  2:3983569/140‑1 (MQ=18)

                                                                                      aGGCGCCACCGGGGCAGCGGGACCACCCGGAGCGACAGGCGCTCCCGGGGACGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                    <  2:2048927/140‑1 (MQ=17)

                                                                                             aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                             <  1:3443703/140‑1 (MQ=255)

                                                                                                   gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                       <  1:1052860/140‑1 (MQ=255)

                                                                                                   gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                       <  1:1870302/140‑1 (MQ=255)

                                                                                                   gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                       >  1:1102511/1‑140 (MQ=255)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACCCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCGGACCAGCCGGAGCGACCgg                                    >  1:3790729/1‑140 (MQ=255)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                    >  2:1644613/1‑140 (MQ=255)

                                                                                                      gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACAAGCCGGAGCGACAgg                                    >  1:635832/1‑140 (MQ=255)

                                                                                                       ccGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCCGGAGCGACAGGCGCAACCGGACCAACCGGAGCGCCGGGAGCGACAGGGGGGACGGGGGACGCCGTACCAGCGGGATCGTCAGGa                                   >  2:2703742/1‑140 (MQ=14)

                                                                                                           ggccacgggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                               >  2:1403214/8‑140 (MQ=18)

                                                                                                           aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  2:62149/96‑1 (MQ=40)

                                                                                                               aCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAATCCGGACCAACCGGAGCGTCGGGAGCCACAGGCGCTACAGGGGAAGTCGGACCCGCAGCAGCGACAGGCGCTACCgg                           >  2:2365529/1‑140 (MQ=18)

                                                                                                               aCCGGAGCACCAGGAGCTCCCGGCGACCCGGGAGCACCCGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCgg                           <  1:3516480/140‑1 (MQ=17)

                                                                                                                cgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                     <  1:776649/111‑1 (MQ=255)

                                                                                                                cgggAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGa                                                     >  2:776649/3‑113 (MQ=255)

                                                                                                                ccGGAGCGACAGGCGCTCCCGGGGCAGCCGGCCCAGCGGGAGCGTCCGGCGCTCCCGGGCAAGCCGGCCCACCCGGAGCGACGGGAGCGAGTGGCGCTAATGGGGACCACGGACCAGCCGGAGCGACAGGAGCTACCggg                          <  1:2489020/140‑1 (MQ=12)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGCCCACCCGGAGCGACGGGAGCCCCAGGGGCTCCGGGGGCAGGCGGACCTGCCGGCGCGACAGGAGCGCCCggg                          >  2:2446146/1‑140 (MQ=17)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTAGGACCAGCCGGAGCGACAGGAGCTACAggg                          >  2:1835574/1‑140 (MQ=255)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGTACGTCGGACCTGCCGGAGCGCCAGCAGCTACAggg                          >  2:2244002/1‑140 (MQ=18)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                          >  2:1725058/1‑140 (MQ=255)

                                                                                                                ccGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAGCAGCCGGAGCGACAGGAGCTACCgcg                          >  2:3368404/1‑138 (MQ=21)

                                                                                                                    aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGGCGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                      >  2:1920156/1‑140 (MQ=255)

                                                                                                                     gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                          <  1:610670/87‑1 (MQ=39)

                                                                                                                     gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                                                          >  2:610670/1‑87 (MQ=39)

                                                                                                                       gACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        >  1:2381249/1‑87 (MQ=255)

                                                                                                                         cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                 <  2:1163566/140‑1 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:319436/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  1:3844175/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  1:1955061/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:2491722/1‑140 (MQ=255)

                                                                                                                              gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  1:2978565/1‑140 (MQ=255)

                                                                                                                              gCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                                                           <  1:2001027/77‑1 (MQ=255)

                                                                                                                              gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCacc            >  2:154563/1‑138 (MQ=255)

                                                                                                                              gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc            >  2:2768869/1‑140 (MQ=255)

                                                                                                                               cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCGACTGGGGACGACGGACCAGCCGGAGACACAGCAGCGACCGGGGAACACGGaaccaca           >  2:1050488/1‑134 (MQ=17)

                                                                                                                               cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc           >  2:529867/1‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCCACAGGGGCTACTGGGGACGTCTGACCAGCCGGAGCGACATGAGCTACCGGGGAAGCCGGACCAACAgg         >  1:3737858/4‑140 (MQ=17)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGACGGACAAACCgg         >  2:491899/4‑140 (MQ=17)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAACCgg         >  2:2120254/4‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg         >  2:3970668/4‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg         >  1:453954/4‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg         >  1:647450/4‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg         >  1:2082985/4‑140 (MQ=255)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCAACTGGGGACGTAGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAACAACCgg         >  2:740477/4‑140 (MQ=17)

                                                                                                                                 actggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCAACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCgg         >  2:2904934/4‑140 (MQ=17)

                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg         >  2:3053316/1‑140 (MQ=255)

                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                             <  2:1073328/72‑1 (MQ=35)

                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcc                                                                             >  1:1073328/1‑72 (MQ=35)

                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCACCCGGACCAACCgc         >  2:3397647/1‑139 (MQ=255)

                                                                                                                                 aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACGGGGGAAGACGGACGAACCgc         >  2:3260774/1‑139 (MQ=255)

                                                                                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                                                        <  2:2764603/75‑1 (MQ=255)

                                                                                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg       <  1:2723872/140‑1 (MQ=255)

                                                                                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg       >  2:3151590/1‑140 (MQ=255)

                                                                                                                                   cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACGGGAGCCACAGGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGACGGACCAACCGGAg       >  2:1500911/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCgcc   >  2:2478173/1‑138 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   >  2:3863479/1‑140 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc   <  1:3151590/140‑1 (MQ=255)

                                                                                                                                       gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGCAAGCCGGACCAACCGCAGCGTCGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGGCCGGGGCCGCCGGACCAACCGGAGCGAc   >  2:701146/1‑140 (MQ=17)

                                                                                                                                        caGCCGGACCCGCCGTAGCGACAGTAGCTCCCGCGGAAGCCGGACCACCCGGCCCGTCGGGCGCCACAGGGGCTACGGGGGACGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAcg  <  1:1752970/139‑2 (MQ=14)

GCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACA  >  NZ_CP034943/607563‑607838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`