BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,853	C→T	7.5%	A232A (GCC→GCT)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,853	0	C	T	7.5%	1206.1 / 44.1	377	A232A (GCC→GCT)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (142/203); new base T (2/26); total (145/232)
Fisher's exact test for biased strand distribution p-value = 1.79e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

AGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACT  >  NZ_CP034943/607715‑607991

 gCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGAGACGGGATCCAAATCATCGACTGCGGCAGTCGGACAAGACGGACCCAAAGGAGCTACCGAGGAAGCCGGACCAAACCGAGCGAAAGGAGCCACGGCAGCaac                                                                                                                                          >  2:1806998/1‑140 (MQ=16)

     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                                      >  1:2763311/1‑140 (MQ=255)

     cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                                      <  1:3053316/140‑1 (MQ=255)

      aGGCGCTCCCGGGGCACCCGGACAAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCTGACCAGCCTGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                                     <  1:489618/140‑1 (MQ=18)

      aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGgg                                                                                                                                     <  1:174516‑M2/140‑3 (MQ=255)

       ggAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                    <  1:529867/140‑1 (MQ=255)

          gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                 <  1:3949752/140‑1 (MQ=255)

             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCCCAGGCACAGGagc                                                                                                                              >  2:950244/1‑140 (MQ=255)

             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCCCAGGCACAGGagc                                                                                                                              >  2:3885999/1‑140 (MQ=255)

             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                              >  2:1968201/1‑140 (MQ=255)

             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                              >  2:583966/1‑140 (MQ=255)

             aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                              >  2:16669/1‑140 (MQ=255)

                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGgcacgtcgcaccagccgg                                                                                                                     <  2:4032272‑M2/140‑19 (MQ=255)

                      gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGtt                                                                                                                     >  2:133013/1‑140 (MQ=255)

                        cGGACCAACCGGACCGTCGGGAGCCACCGGGGCTACCGGGGCCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                                   <  2:3790729/140‑1 (MQ=25)

                          gACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATcc                                                                                                                 >  1:1482892/1‑140 (MQ=255)

                              aaCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAAt                                                                                                             >  1:1942620/1‑140 (MQ=255)

                               ccccgcgCGACGGTCGCCTCCGGCGCTCCGGGGGACGTCGGACCAGCCGTAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  2:853302/134‑1 (MQ=21)

                               aCCGGAGCGACTGGTGCCACTGGGGCTCCGGGTGACGTCGGAGCCCCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:2024123/140‑1 (MQ=25)

                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGgcacgtcggaccagccggagccacagg                                                                                                            <  2:1436054‑M2/140‑28 (MQ=255)

                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:4175472/140‑1 (MQ=255)

                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:1436054/140‑1 (MQ=255)

                               aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:1835574/140‑1 (MQ=255)

                                ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                                           >  2:1097156/1‑140 (MQ=255)

                                ccGGAGCCCCGGGACCCACCGGACCTACCGGGGTGCCGGGACCAGGGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                                           <  2:3044837/140‑1 (MQ=18)

                                  ggAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACgg                                                                                                         >  1:3214152/1‑140 (MQ=255)

                                   gAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccagccggagcgacaggagct                                                                                                        >  1:3178306‑M2/1‑109 (MQ=255)

                                    cccgtcGGGATCCACAGGGGCTTCTGGGGAGGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:3619932/135‑1 (MQ=25)

                                    agctccGGGAGCCACAGGGGCTCCCGGGGACGCAGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:2652994/135‑1 (MQ=21)

                                    agctccGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:4062676/135‑1 (MQ=255)

                                    agcgtcgggagcgacAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:1147926‑M2/125‑1 (MQ=255)

                                    agccaccggagcgacAGGGGCTAATGGGGACGTCGGAACAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  2:2077550‑M2/125‑1 (MQ=255)

                                    aGCGAGGGTAGACACCCGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:616451/140‑1 (MQ=25)

                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:955555/140‑1 (MQ=255)

                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:133013/140‑1 (MQ=255)

                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:2491722/140‑1 (MQ=255)

                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  2:3214152/140‑1 (MQ=255)

                                    aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:2214412/140‑1 (MQ=255)

                                      cGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtat                                                                                                     <  2:3944940/140‑1 (MQ=255)

                                         cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggaggtcggaccagccggagcgacaggagctaccggg                                                                                                  <  2:232793‑M2/140‑38 (MQ=255)

                                                 acAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                                            >  2:2678551‑M2/3‑106 (MQ=255)

                                                    ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                                       <  2:3844175/140‑1 (MQ=255)

                                                       gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                    >  2:1486095/1‑140 (MQ=255)

                                                       gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                    >  2:2157003/1‑140 (MQ=255)

                                                        cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       >  2:1450102/1‑120 (MQ=255)

                                                         tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTc                                                                                  >  2:3525358/1‑140 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCCCAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  2:1001931/1‑140 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  2:2520507/1‑140 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  2:2936228/1‑140 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  2:2908129/1‑140 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 <  2:4218807/140‑1 (MQ=255)

                                                          aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                 >  1:1383404/1‑140 (MQ=255)

                                                           ccggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCAACAGGCCCAGGCGCAGCAGGGTTATCCGAATACGGCTAGAGATATAACTTAAGCGCTCaa                                                                                >  2:688950/3‑140 (MQ=18)

                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:3898967/140‑1 (MQ=255)

                                                                gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:1790662/140‑1 (MQ=255)

                                                                gACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCATCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                           <  1:3389080/140‑1 (MQ=255)

                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            >  2:3650090/1‑106 (MQ=255)

                                                                 aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                          <  2:4089934/140‑1 (MQ=255)

                                                                   gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatatat                                                                                               <  2:1865145/117‑1 (MQ=255)

                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTAGATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCt                                                              >  2:640688/1‑140 (MQ=255)

                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcac                                                                                                                                          <  1:241810/60‑1 (MQ=255)

                                                                                  gCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTCTAGATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGt                                                         >  2:2278800/1‑140 (MQ=255)

                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       <  1:2380566/90‑1 (MQ=255)

                                                                                       aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                       >  1:2949657/1‑89 (MQ=255)

                                                                                            cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGAt                                               >  1:780097/1‑140 (MQ=255)

                                                                                            cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGAt                                               >  1:1238987/1‑140 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                                   <  1:3812222/70‑1 (MQ=255)

                                                                                              aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTc                                             >  2:3415434/1‑140 (MQ=255)

                                                                                               ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCa                                            >  1:1012036/1‑140 (MQ=255)

                                                                                                cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                           <  1:2069767/140‑1 (MQ=255)

                                                                                                   ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAg                                        <  2:2731418/140‑1 (MQ=255)

                                                                                                    gAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAGTACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATCGAGGCTGATGTCATTTTTGATTCAACAgg                                       >  1:3771616/1‑140 (MQ=255)

                                                                                                        ccGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATa                                   <  2:453954/140‑1 (MQ=255)

                                                                                                        ccGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATa                                   <  2:2997048/140‑1 (MQ=255)

                                                                                                        ccGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATa                                   <  2:3982116/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATacc                                 <  1:869227/140‑3 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:3566320/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:16642/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:2923311/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:4095932/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:1457570/140‑1 (MQ=255)

                                                                                                          ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                 <  1:2520507/140‑1 (MQ=255)

                                                                                                            aCCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                               >  1:2575652/1‑140 (MQ=255)

                                                                                                            aCCAACCGGACCCACACGAGCCACGGGAGCCACAGGCACAGCAGCAGATGGGTTATCCGAATACGGGTATATATATAACTTAAGCTCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                               <  2:2605118/140‑1 (MQ=255)

                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  1:1615059/140‑1 (MQ=255)

                                                                                                                aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                           <  2:174516/140‑1 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            <  1:4285397/58‑1 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                            >  2:4285397/1‑58 (MQ=255)

                                                                                                                 ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTCTAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCa                          >  2:4173737/1‑140 (MQ=255)

                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGTTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                      <  2:2409387/140‑1 (MQ=255)

                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACTGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                      <  2:3737858/140‑1 (MQ=255)

                                                                                                                     aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                      <  1:3080435/140‑1 (MQ=255)

                                                                                                                            tgtggcACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca               <  2:3612719/135‑1 (MQ=255)

                                                                                                                            ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca               <  1:2341001/140‑1 (MQ=255)

                                                                                                                            ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca               <  2:3145187/140‑1 (MQ=255)

                                                                                                                            ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca               <  1:3326604/140‑1 (MQ=255)

                                                                                                                             gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt              <  1:1703516/140‑1 (MQ=255)

                                                                                                                             gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt              <  1:3525358/140‑1 (MQ=255)

                                                                                                                                ccACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGcc           >  2:2463869/1‑140 (MQ=255)

                                                                                                                                  aCGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGcccc         >  1:3743861/1‑140 (MQ=255)

AGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACT  >  NZ_CP034943/607715‑607991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`