Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,856 A→T 6.1% T233T (ACA→ACT EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,8560AT 6.1% 1252.8 / 35.0 378T233T (ACA→ACTEO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (142/208);  new base T (2/22);  total (146/232)
Fisher's exact test for biased strand distribution p-value = 9.85e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 395 total aligned reads displayed.

CGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTTCTC  >  NZ_CP034943/607717‑607995
                                                                                                                                           |                                                                                                                                           
cGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcaca                                                                                                                                             <  1:3041748/140‑1 (MQ=255)
   cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggc                                                                                                                                          >  1:3546437/1‑140 (MQ=255)
    aGGCGCTCCCGGGGCACCCGGACAAACCGGAGCGACGGGAGCCACAGGGGCTACGGGGGACGTCTGACCAGCCTGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggca                                                                                                                                         <  1:489618/140‑1 (MQ=18)
    aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGgg                                                                                                                                         <  1:4167681‑M2/140‑3 (MQ=255)
    aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACAGGgg                                                                                                                                         <  1:376538‑M2/140‑3 (MQ=255)
     ggAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcac                                                                                                                                        <  1:529867/140‑1 (MQ=255)
       aGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGCAGCGACGGGAGCCACAGGGGCTACTGGggacg                                                                                                                                      <  2:62749‑M2/140‑6 (MQ=255)
        gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgt                                                                                                                                     <  1:85153‑M2/140‑7 (MQ=255)
        gCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                                                                                                     <  2:4194183/140‑1 (MQ=255)
          tACCGGGGAAGCCGGCCCAACCGGAGCCCCGGGCGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGag                                                                                                                                   <  1:998686/140‑1 (MQ=18)
           aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCCCAGGCACAGGagc                                                                                                                                  >  2:3885999/1‑140 (MQ=255)
           aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                                                                                                  >  2:1705936/1‑140 (MQ=255)
             cGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                                                <  1:1403214/140‑1 (MQ=255)
                ggAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggacgtcggaccag                                                                                                                             <  2:20637‑M2/140‑15 (MQ=255)
                    gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGtt                                                                                                                         >  1:3079958/1‑140 (MQ=255)
                    gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGgcacgtcgcaccagccgg                                                                                                                         <  2:4032272‑M2/140‑19 (MQ=255)
                    gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGtt                                                                                                                         >  2:955555/1‑140 (MQ=255)
                      cGGACCAACCGGAGCCACGGGAGCCCCCGGGGCTCCTGGGGACGGCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                                       <  1:1777928/140‑1 (MQ=18)
                      cGGACCAACCGGACCGTCGGGAGCCACCGGGGCTACCGGGGCCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                                                                                                       <  2:3790729/140‑1 (MQ=25)
                        gACGAAGCGGAGCCCCTGGAGCCACTGGCGCTCCGGGTGAGGTCGGCGGCGCCGGAGCTTCCGGAGATCCCGGTGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATcc                                                                                                                     <  1:2006082/140‑1 (MQ=16)
                        gACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATcc                                                                                                                     >  1:1482892/1‑140 (MQ=255)
                             tccGGAGCGCCGGGAGCCACAGGTGCTACTGGGGACGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:266739/139‑1 (MQ=255)
                             aCCGGAGCGCCGTCCGCCACAGGTCCTACTGGGTAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:1657203/140‑1 (MQ=18)
                             aCCGGAGCGACGGGTGACTCCGGCGCTCCGGGTGACGTCGGAGCCCCCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:1810070/140‑1 (MQ=25)
                             aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:16990/140‑1 (MQ=255)
                             aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:3934263/140‑1 (MQ=255)
                              ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                                               >  2:2220887/1‑140 (MQ=255)
                              ccGGAGCGACGGGAGCCACAGGGGATACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                                               <  1:3155683/140‑1 (MQ=255)
                              ccGGAGCCCCGGGACCCACCGGACCTACCGGGGTGCCGGGACCAGGGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                                                                                               <  2:3044837/140‑1 (MQ=18)
                                  cgCGACGGGAGCCTCAGGTGCTTCTGGTGACGTCGGACCCCCGGGAGCGACAGGAGCTACCGGGGAAGCAGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGTCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  1:2533542/139‑1 (MQ=25)
                                  accctcGGTAGCCACAGGTGCTTCCGGGGAACTCGGACCATCCGTAGCGACAGGAGCTACGGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:643843/135‑1 (MQ=25)
                                  aGCGAGGGTAGACACCCGTGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  1:616451/140‑1 (MQ=25)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGACTACGGGt                                                                                                           >  2:1065808/1‑140 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:3214152/140‑1 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:1955061/140‑1 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:4240960/140‑1 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:512377/140‑1 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  1:1134989/140‑1 (MQ=255)
                                  aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  1:2417932/140‑1 (MQ=255)
                                   gCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGta                                                                                                          <  1:3260774/140‑1 (MQ=255)
                                    ccgcgggagcgacAGGCGCTACCGGGGAAGGCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtat                                                                                                         <  2:3679351‑M2/127‑1 (MQ=255)
                                    cGACGGGAGCCACAGGGGCTTCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtat                                                                                                         <  1:949484/140‑1 (MQ=255)
                                    cGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtat                                                                                                         <  2:3944940/140‑1 (MQ=255)
                                       cGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGggccgtcggaccagccggagcgacaggagctaccggg                                                                                                      <  2:232630‑M2/140‑38 (MQ=255)
                                               acAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                                                                                                >  2:2678551‑M2/3‑106 (MQ=255)
                                                  ggggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAg                                                                                           <  2:3844175/140‑1 (MQ=255)
                                                     gCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAgcgc                                                                                        >  2:2141470/1‑140 (MQ=255)
                                                       tACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTc                                                                                      >  2:3525358/1‑140 (MQ=255)
                                                        aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                     >  2:3741757/1‑140 (MQ=255)
                                                        aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                     >  2:2908129/1‑140 (MQ=255)
                                                        aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa                                                                                     >  2:2520507/1‑140 (MQ=255)
                                                           ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc                                                                                  <  1:3856569/140‑1 (MQ=255)
                                                             ggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACgg                                                                                >  1:2146770/1‑140 (MQ=255)
                                                              gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                               >  2:1256048/1‑140 (MQ=255)
                                                              gACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt                                                                               <  1:1232069/140‑1 (MQ=255)
                                                               aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:2947890/106‑1 (MQ=255)
                                                               aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                              >  1:4089934/1‑140 (MQ=255)
                                                               aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa                                                                              <  1:319436/140‑1 (MQ=255)
                                                                cGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg                                                                             <  1:1317796/140‑1 (MQ=255)
                                                                 gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatatat                                                                                                   >  1:3987614/1‑117 (MQ=255)
                                                                 gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGtatatatat                                                                                                   >  1:1865145/1‑117 (MQ=255)
                                                                 gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGc                                                                            >  2:4301703/1‑140 (MQ=255)
                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCta                                                                          >  2:1620846/1‑140 (MQ=255)
                                                                                          cTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGAt                                                   >  1:780097/1‑140 (MQ=255)
                                                                                            aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTc                                                 >  2:3415434/1‑140 (MQ=255)
                                                                                            aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTc                                                 >  1:3381508/1‑140 (MQ=255)
                                                                                             ccGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCa                                                >  1:1012036/1‑140 (MQ=255)
                                                                                              cGGGTAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                               <  1:2904934/140‑1 (MQ=255)
                                                                                              cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                               <  2:4153868/140‑1 (MQ=255)
                                                                                              cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                               <  1:2069767/140‑1 (MQ=255)
                                                                                              cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCaa                                               >  2:746939/1‑140 (MQ=255)
                                                                                                gggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACa                                             <  1:1734228/140‑1 (MQ=255)
                                                                                                 ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAg                                            <  2:1861227/140‑1 (MQ=255)
                                                                                                 ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAg                                            <  2:2731418/140‑1 (MQ=255)
                                                                                                 ggAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAg                                            >  2:853736/1‑140 (MQ=255)
                                                                                                     gCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGTGCAGCTGGGTTATCCGAATAGGGGTCTATATATAACTTTAGCGCTCGATCGGTAGCTATTGAGGGTGATGTCATTTTTGATTGAACTGGGAt                                        >  2:1355567/1‑140 (MQ=255)
                                                                                                     gCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTCTATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGAt                                        >  1:2377389/1‑140 (MQ=255)
                                                                                                      ccGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATa                                       <  2:2997048/140‑1 (MQ=255)
                                                                                                      ccGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATa                                       <  2:3982116/140‑1 (MQ=255)
                                                                                                        ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                     <  1:1383160/140‑1 (MQ=255)
                                                                                                        ggACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAac                                     <  1:1457570/140‑1 (MQ=255)
                                                                                                          aCCAACCGGACCCACACGAGCCACGGGAGCCACAGGCACAGCAGCAGATGGGTTATCCGAATACGGGTATATATATAACTTAAGCTCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAacca                                   <  2:2605118/140‑1 (MQ=255)
                                                                                                              aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                               <  1:2615109/140‑1 (MQ=255)
                                                                                                              aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                               <  1:34445/140‑1 (MQ=255)
                                                                                                              aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                               <  1:1615059/140‑1 (MQ=255)
                                                                                                              aCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCAcccc                               <  2:2381188/140‑1 (MQ=255)
                                                                                                               ccGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                                                                                                <  1:4285397/58‑1 (MQ=255)
                                                                                                                   agtgACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                          <  1:2290430/137‑1 (MQ=255)
                                                                                                                   aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                          <  1:3794529/140‑1 (MQ=255)
                                                                                                                   aGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                          <  1:248087/140‑1 (MQ=255)
                                                                                                                       aCAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                                                                                           <  2:1797019/55‑1 (MQ=255)
                                                                                                                         aGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTacac                    >  2:1466449/1‑140 (MQ=255)
                                                                                                                          ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                   <  2:3632322/140‑1 (MQ=255)
                                                                                                                          ggAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTAcaca                   <  2:3145187/140‑1 (MQ=255)
                                                                                                                           gAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACAt                  <  2:1440863/140‑1 (MQ=255)
                                                                                                                              ccACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGcc               >  2:2941702/1‑140 (MQ=255)
                                                                                                                              ccACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGcc               >  2:2463869/1‑140 (MQ=255)
                                                                                                                                aCGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGcccc             >  1:3739649/1‑140 (MQ=255)
                                                                                                                                 cGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGaa                          <  1:3573938/126‑1 (MQ=255)
                                                                                                                                           aggcacaggAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTtctc  >  1:2763021/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGCTATAGAGGCTGATGTCATTTTTGATTCAACAGGGATAACCACCCCAGGAATTACACATGCCCCTGGAACTTCTC  >  NZ_CP034943/607717‑607995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: