Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 9.9% Q22H (CAA→CAC EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC 9.9% 519.8 / 6.1 229Q22H (CAA→CACEO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (151/40);  new base C (4/31);  total (155/74)
Fisher's exact test for biased strand distribution p-value = 2.03e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755392‑755669
                                                                                                                                           |                                                                                                                                          
cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                            >  1:786664/1‑140 (MQ=255)
cACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCaa                                                                                                                                            >  1:4332609/1‑140 (MQ=255)
  ccGTTTTTTTCATGGTAAGAGCTGTAATTGTTTGATGCCATATTAAACTAAAAAAACGAGGGGGAATGAGAAATGGAAAATCACATTTATCTTGCTTGCGATTAAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                          <  2:3191615/140‑1 (MQ=255)
  ccGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgg                                                                                                                                          <  1:4015092/140‑1 (MQ=255)
        ttttCAAGTGAATAGCTGTCCTTGTTTGATGGCATATGTAACGAAAACAACGAGAGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCATCTGTTCATACGTATGGAGACTGCAGAAATGTAGGGGGGTg                                                                                                                                    <  2:2513681/140‑1 (MQ=255)
        ttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTg                                                                                                                                    >  1:4171303/1‑140 (MQ=255)
               gggAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACACCACAAATCAAGGGGGGTGGGGGCAg                                                                                                                             >  2:3871926/1‑140 (MQ=255)
                     agCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCATGCTGGCGATTCAACTGTTCAAACGTATTGATACAGCACAAATCAAGCGGGGTGGGGGCAGTAtctc                                                                                                                       >  2:3386845/1‑140 (MQ=255)
                        tttCCATTATTGATGCAATATGAAACCAAAAAAACGAGGGGTAATGAGAAATGGCAAATCACATTTATCTTGTTGGCGATTCAACTGTACAACCGTATGGCGCCAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGc                                                                                                                    <  1:2007977/138‑1 (MQ=255)
                        tgtcactgatttatGCCAAATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATAACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACACCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGc                                                                                                                    <  1:1090131/134‑1 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAATCGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:1663017/1‑140 (MQ=255)
                          tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:2926774/1‑140 (MQ=255)
                          tCCATGATTGATGCCAGATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGGTCAAACGTATGGAGACAGCACCAATCACGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:1598393/1‑140 (MQ=255)
                            cATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCACACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGc                                                                                                                >  2:526831/1‑140 (MQ=255)
                             aTGATTGATTCCATATGAAACGAAAAAAACGAGGGGGAATTAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCa                                                                                                               <  2:557354/140‑1 (MQ=255)
                                  ttattccATTTGAAACTAAAAAAACGAGGGTGAATGAGAAATCGCAAATCACTTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGc                                                                                                          <  2:2868224/135‑1 (MQ=255)
                                   tattaattatGAAACGAAAAAAACTAGTCGGAATTTTAAATGTCTATTCTTTTTTATGTTGCTGTGTATTAACCAATACAAACGTTTCTATACAGCTAAAATCCATGCGGCTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                         <  2:1082390/133‑1 (MQ=255)
                                   gatCCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGCCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTAGGGACCCAGCACACATCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                         <  1:2806628/140‑1 (MQ=255)
                                    atGCGATATGAAAGGAAAAAAACGAGGGGGAAAGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACCGCAGACATCAAGGGGGGTGGGGGCAGGTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:219430/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCgg                                                                                                       >  1:2483341/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTCTGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTGCGGGGGTCGCATCTGCCg                                                                                                        >  2:3598147/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:4092645/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:3898953/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:3181450/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:3260394/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:3556194/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:2056466/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:2450516/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:1240939/1‑140 (MQ=255)
                                    atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGAGTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCCTCTGCCg                                                                                                        >  2:3559843/1‑140 (MQ=255)
                                      tcactatGAAACGAAAAAAACGAGGGGGAATGAGAAATGCCAAATCACATTTATCTTGCTGGCGAGTCACCTGTTCAACCGTATGGAGACAGCACAAAGCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  1:3363291/136‑1 (MQ=255)
                                      tacctatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTTGCTATTCAACTGTTCAAACGTATGGAGACAGCACAACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  2:2898806/136‑1 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGCGGGGGATTGATAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  2:2562792/140‑1 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACCGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      >  1:2781814/1‑140 (MQ=255)
                                      gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      >  1:1011929/1‑140 (MQ=255)
                                         atatTAAACGAATAATACGAGGTGGAATGAGAAATGTCAAATCACATTTATGTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAAGCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCa                                                                                                   <  2:2306706/140‑1 (MQ=255)
                                         atatTAAACGAAAAAAACTAGTGTGTTTGAGATATGGCTAATCACATTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACACCACACACCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCa                                                                                                   <  2:2168496/140‑1 (MQ=255)
                                         atatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCa                                                                                                   >  1:2630366/1‑140 (MQ=255)
                                          tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                  >  1:4125357/1‑140 (MQ=255)
                                              caaCCAAAAAAACGAGGGGGAACGAGAAACGGCAACTCACATTTAGCGTGCTGGCGATTCACCTGCTCAAACGTATGCAGACAGCACAAATCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:3667022/139‑1 (MQ=255)
                                              aaaaGAAAAAAACGAGGGTGAATTAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAACCACGGCGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:2984811/140‑1 (MQ=255)
                                              aaaCGAAAAAAACGAGGGGGAATGATATATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGGATGGAGACAGCACCAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:313852/140‑1 (MQ=255)
                                              aaaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACTTTTATCTTGCTGGCGATTCAACTGTTCAAAGGTATGGAGAAACCACAAATCAAGGGGCGTGGGGGCATTGTCTCGGCTCGAAACTGACGGAGCATAtta                                                                                              >  2:2748905/1‑139 (MQ=255)
                                              aaaCGAAAAAAACGAGGGGGAAAGAGAAATCGCAAATCACCTGTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAAGCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:3712949/140‑1 (MQ=255)
                                              aaaCCAAAAAAACGAGGGCGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACAACTCAAGGGGGGCGGGGGCAGGTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:5405/140‑1 (MQ=255)
                                                acccaaacaaCGAGGCGGACTGAGAAAGGGCAAATCACATTTATCGTGCTGGCGATTCAACTGTTCACCCGTCTGGAGCCAGCACAACTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  2:2056466/136‑1 (MQ=255)
                                                accaaaaaaaCGAGGCGGAAGGACAAATCGCAAATCACCTTTATCTGGCGGCCCATTCAACGGTTCACCCGTATGGCGACAGCACAAATCACGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  1:465226/137‑1 (MQ=255)
                                                aCGAAAAAAACGACGGGGAATGAGAAATGGCAAATCACATATATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            >  2:1764790/1‑140 (MQ=255)
                                                 ccaaaaaaaCGAGGGGGACTGACCCATGGCAAATCACATGTATCTTGCTGGCGATTCAACTGTTCAACCGTATGGACACAGCACACAGCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAg                                                                                           <  1:2355905/138‑1 (MQ=255)
                                                        aaCGAGGGGCAACGACAAATGCCAAATCCCAATTATCTTGCTGGCGACTCCACTCTACCACCGTCTCGACACACCACAAACCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                    <  1:3768438/140‑1 (MQ=255)
                                                              ggggACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGc                                                                              >  1:13979/1‑140 (MQ=255)
                                                              ggggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGc                                                                              >  1:236036/1‑140 (MQ=255)
                                                              ggggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTAAGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGc                                                                              >  1:887168/1‑140 (MQ=255)
                                                                ggAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCaa                                                                            >  2:3044117/1‑140 (MQ=255)
                                                                           tGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCCAGTGATTAACAGAGCAATCGGGGgacga                                                                 >  2:3449561/1‑140 (MQ=255)
                                                                            ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                                >  1:1806681/1‑140 (MQ=255)
                                                                                  tCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGa                                                          >  1:3012305/1‑140 (MQ=255)
                                                                                                cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  2:1396971/1‑140 (MQ=255)
                                                                                                  ggCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGATCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAaga                                          >  1:854955/1‑140 (MQ=255)
                                                                                                  ggCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAaga                                          >  1:190767/1‑140 (MQ=255)
                                                                                                  ggCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAaga                                          >  1:189420/1‑140 (MQ=255)
                                                                                                     gacacaccTGCTCACACGTATGGCGACCCGACCACTCACCGGCGGTCGCGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGAGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                                       <  1:1463633/133‑1 (MQ=255)
                                                                                                     gATTCTACTTTTCAAACGTATGGAGACAGCACAACTCCCGCGCGGTGGGGGCCGTTTCTCGGCTCGCATCTTCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAggg                                       <  2:4076775/140‑1 (MQ=255)
                                                                                                       ttCAACTGTTCAAACGTATGGACACAGAACACATCAAGGCGGGTGGGTGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCa                                     <  2:1484320/140‑1 (MQ=255)
                                                                                                            ctcttCAACCGTATGGACACAGCACACCTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCtt                                <  2:2798983/137‑1 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:3836552/1‑140 (MQ=255)
                                                                                                               ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:3633258/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           >  1:4305858/1‑140 (MQ=255)
                                                                                                                 cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           >  2:4024880/1‑140 (MQ=255)
                                                                                                                       tATGTAGACAGCACCAATCCAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     <  2:1572997/140‑1 (MQ=255)
                                                                                                                       tATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     >  2:6665/1‑140 (MQ=255)
                                                                                                                       tATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     >  2:224761/1‑140 (MQ=255)
                                                                                                                       tATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     >  2:613848/1‑140 (MQ=255)
                                                                                                                       tATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     >  2:4266669/1‑140 (MQ=255)
                                                                                                                       gaTGGAGACACCACCACTCCAGGGCGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCt                     <  2:3466119/139‑1 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      >  2:3696634/1‑57 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  2:2854362/1‑140 (MQ=255)
                                                                                                                         tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:1218867/1‑140 (MQ=255)
                                                                                                                              aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg              >  1:2892994/1‑140 (MQ=255)
                                                                                                                              aCAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAGCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAtgtg              >  1:3524281/1‑140 (MQ=255)
                                                                                                                                aaaacaAATCAAGCGGGGTGGGTGCATTTTATAGACACGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:1938425/4‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:841500/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:1798716/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:1357301/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:1141240/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:101760/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:958607/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:618270/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:4257407/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:2282035/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:1769466/1‑140 (MQ=255)
                                                                                                                                aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:1391407/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  2:2750559/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  2:1875578/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  1:1266366/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  1:3076593/1‑140 (MQ=255)
                                                                                                                                  cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  1:2160128/1‑140 (MQ=255)
                                                                                                                                       aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg     >  2:3325466/1‑140 (MQ=255)
                                                                                                                                       aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg     >  2:3796866/1‑140 (MQ=255)
                                                                                                                                          aaGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAt  >  1:1022687/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755392‑755669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: