Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 822,230 T→G 6.2% A252A (GCT→GCG EO946_RS04190 → sodium:alanine symporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943822,2300TG 6.2% 716.9 / 2.1 283A252A (GCT→GCGEO946_RS04190sodium:alanine symporter family protein
Reads supporting (aligned to +/- strand):  ref base T (106/143);  new base G (31/2);  total (138/145)
Fisher's exact test for biased strand distribution p-value = 6.54e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

AGTAACAGAGTTTGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCAA  >  NZ_CP034943/822095‑822368
                                                                                                                                       |                                                                                                                                          
aGTAACAGAGTTTGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTg                                                                                                                                        <  2:671530/140‑1 (MQ=255)
 gTAACAGAGTTTGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTGTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTGTGTGTTTTCTTTTCGAATGGGTTTACCTGTTCTTCAGCtggtgg                                                                                                                                       >  1:3146986/1‑140 (MQ=255)
            tGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTg                                                                                                                            >  1:2990569/1‑140 (MQ=255)
            tGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTACAGCGGTTGGAGGATTCGCTg                                                                                                                            >  2:831467/1‑140 (MQ=255)
            tGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTAACTCTTCTTGAGCTGTTGGAGGATTCGCTg                                                                                                                            >  1:2579540/1‑140 (MQ=255)
                cgcccTCGTATGGCAGGGGCCTCCGTGGGAGCCGCTCTCCACCGTGTTCTGCTGACCCTTTCAGCAGTGCCGGCGTTTTTCTCTCTGATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTGGGAGGATTCGCTGgcgc                                                                                                                        <  1:2499428/137‑1 (MQ=255)
                  gCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGgcg                                                                                                                         >  2:2600658/1‑137 (MQ=255)
                  gCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGgcg                                                                                                                         >  2:3895183/1‑137 (MQ=255)
                  gCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTAAGCTGTTGGAGGATTCGCTGGCGCTg                                                                                                                      >  2:4102430/1‑140 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  1:1962158/140‑1 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  1:689634/140‑1 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  2:370780/140‑1 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  1:1982337/140‑1 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  1:1744580/140‑1 (MQ=255)
                    ctctTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGcc                                                                                                                    <  2:1956000/140‑1 (MQ=255)
                               ggggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTGGCGCGTATCTTTTAGAATGCGTTTAACTCTTCTTCAGCGGTTGGGGGATTCGCTGGCGCTGCCCTGGCTGAAAc                                                                                                         >  1:2189031/1‑140 (MQ=255)
                               ggggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTGTTTGTCTCTTATCTTTTAGAATGCGTTTAACTCTTCTTAAGCGGTTTGAGGATTAGCTGGCGCTGCCCTGGCTGAAAc                                                                                                         >  1:1205384/1‑140 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTTTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTATCAGTTATCTTTTAGAAATCGTTTAAATAATAGTCATCTGTTGGATGATTATCTTGCGCTGCCCTGGCTGAAAcc                                                                                                        >  1:4209349/1‑140 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTGTCTCTTATCTTTTCGAATTCGTTTACCGATTCTTCAGCTGTTTGAGGATTAGCTGGCGCTGCCCTGGCTGaaaac                                                                                                        >  1:2218992/1‑138 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTGTCTTATCTTTTCCAATTCGTTTACCTCGTATTAAGCTGTTGGATGATTACCTGGCGCTGCCCTTGCTGAAAcc                                                                                                        >  1:3928626/1‑140 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTGTTCAGCGGGTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAAcc                                                                                                        >  1:1936670/1‑140 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGgcg                                                                                                                         >  2:4299952/1‑123 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGgcg                                                                                                                         <  1:4279093/123‑1 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGTAAcc                                                                                                        >  2:2651802/1‑140 (MQ=255)
                                gggCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTATCTCTTATCTTTTAGAATTCGTTTAACTCTTCTTCAGGGGTTGGAGGATTAGCTGGCGCTGCCCTGGCTGAAAcc                                                                                                        >  1:2765965/1‑140 (MQ=255)
                                 ggCATACGTGGGAGCCCGTCTCCTCATTGTACTGATTAACCTTTCATCAGTGCAGTCGTTTTTCTCTCTTATCTTTTAGAATTGGTTTAACTCTTCTTCAGCTGATGGATGATTCCCTTGCGCTGACCTGGCTTAAACCg                                                                                                       >  2:1823395/1‑140 (MQ=255)
                                     tACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTGTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCg                                                                                                   <  1:898050/140‑1 (MQ=255)
                                       cGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTGGTATCTTTTCGAATTCGTTTAACTCTTCTTGAGCGTTTGGAGGATTCGCTGGCCCTGCCCTGGCTTAAACCGTTCggg                                                                                                 >  1:4065887/1‑138 (MQ=255)
                                       cGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTAGAATTCGGTTACCTCTTCTTCAGCGGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCt                                                                                                 >  1:3649854/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGGGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTgggg                                                                                             >  2:3169967/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTGGCTgggg                                                                                             >  2:922480/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCCAATGCGTTTACCTCTTCTAAAGCAGTTGGAGGATTCGCTGGCGCTGCCCATGCTGAAACCGGTCGCGgggg                                                                                             >  2:3012161/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATATTTTAGAATTCGTTTACCTCTTCTTCAGCTGTTTGAGGATTCGCTGGCGCTGCCCTGGCTGTAACCGTTCGCGgggg                                                                                             >  1:1732101/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTACCTTTTCGAATGCGTTTACCTCTTCTTCAGGTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGGTCGCTgggg                                                                                             >  2:605964/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCGCGGCTCTGTTCGCATGGGGGTTCCTCTTCATCAGCGGTTGGAGGATTGGCTGGCGCTGGGCTGGGTGGAAACGTTGGCTgggg                                                                                             >  2:3375544/1‑140 (MQ=255)
                                           ggAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCACTCTTATATTTTCGAATTCGTTTACCGCTTCTTCAACTGTTGGAGGATTCGCATGCGCTGCCCTGGCAGAAACCCTTCGgggggg                                                                                             >  1:3059628/1‑140 (MQ=255)
                                            gAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTATCTCTTATCTTTTCTAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGa                                                                                            >  2:1416501/1‑140 (MQ=255)
                                               ccGGTCTCCTCATTGTTCTGATGAACCTTTCATCCGTGCCGGCGTTTTTGTCTCGTATCTTTTCGAATTCGGTTAAATGTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCAGGCTGAAAACGTGCGCTGGGGAttt                                                                                         >  1:1906948/1‑140 (MQ=255)
                                               ccGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCGGGGGAttt                                                                                         >  2:3420151/1‑140 (MQ=255)
                                                 ggTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCCCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGc                                                                                       >  2:1664221/1‑140 (MQ=255)
                                                  gTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTGTCTCTTATCTTTTGGAATGCGTTTACCTCTTCTTGAGCGGTTGGAGGATTGGGTGGCGCGGCCCGGGCTGAAACCGTTCGCGGGGGATTTTcc                                                                                      >  1:79695/1‑140 (MQ=255)
                                                     tcctcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCcgc                                                                                   <  2:1311113/140‑1 (MQ=255)
                                                        tcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCggg                                                                                <  1:362867/140‑1 (MQ=255)
                                                        tcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCggg                                                                                <  1:498165/140‑1 (MQ=255)
                                                        tcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCggg                                                                                <  1:2672184/140‑1 (MQ=255)
                                                        tcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCggg                                                                                <  1:3409806/140‑1 (MQ=255)
                                                        tcATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCGGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCggg                                                                                <  1:204405/140‑1 (MQ=255)
                                                          aTTGTTCTGATGAACCTTTCATCAGTGCCGGCGTGTTTCTCTCTTATCTTGTCGAATGCGTTTACCTCTTCTTCATCTGTTGGAGGATTCGGTGGCGCTGCCCTGGCTTAAACCGTTCTCTGGTGATTTGCCCGCGGGCt                                                                              <  2:1240916/140‑1 (MQ=255)
                                                          aTTGGTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTAACTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCt                                                                              <  2:2160670/140‑1 (MQ=255)
                                                           ttGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTg                                                                             <  2:1579010/140‑1 (MQ=255)
                                                           ttGTTCTGATGAACCTTTCATCAGTGCCGGCGTGTTTCTCTCTTATCTTTTCGAATTGGGTTACCTCTTCTTGCGCGGTTGGAGGCTTCGCTGGCGCTGCCCGGGCTGAAAGCGTTAGCGGGGGATTTGGCCGCgggggg                                                                             >  1:3535537/1‑137 (MQ=255)
                                                            tGTTCTGATGAACCTTTCATCAGTGCCGGCGTGTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGCTTTGCCCGCGGGCTGt                                                                            >  2:3773960/1‑140 (MQ=255)
                                                                 tgatgaACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCt                                                                       >  2:2969054/1‑140 (MQ=255)
                                                                 tgatgaACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTAATCTGTTTGAGGATTCGCTGGCGCTGCCCAGGCTGAAACCGTTCGGGGGGGAGTTGCCCGCGGGCGGTATTCt                                                                       >  2:57483/1‑140 (MQ=255)
                                                                   atgaACCTTTCATCAGTGCCGGCGTTTTTGTCTCTTATCTTTTCGAATGCGTTTACCGCGTCTTCAGCGGTTGGGGGATTGGCTGGCGCGGCCCTGGCGGGAACCCTTCGCGGGGGATTTTCCCGCGGGCTGTATTCTaa                                                                     >  1:710105/1‑140 (MQ=255)
                                                                   atgaACCTTTCATCAGTGCCGGCGTTTTTGTCTCTTATATTTTGGAAATCGTTTTACACTTCTTAAGCTGTTTGAGGATTGGCTGGCGCTGCCCTGGCTGAAAACCTTCGCTGGGGCTTTTGCCCCGGGCGGTATTGTaa                                                                     >  1:3276462/1‑140 (MQ=255)
                                                                   atgaACCTTTCATCAGTGCCGGCGTTTTTGTCGGTTATCGTTTCGAATGCGTTTACCTCATCTTAAGCTGTTGGAGGATTGGCTGGGGCTGCCCGGGCGGAAACCGGTCGCGGGGGATTTTGCCGCGGGCGGTATTGTaa                                                                     >  1:2874718/1‑140 (MQ=255)
                                                                   atgaACCTTTCATCAGTGCCGGCGTTTTACACTCTTATCTTGTCGAATTCTTTTACCTCTTCTTGAGCTGTTGGAGGATTCGCATGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTTCCCGCGGGCTGTATTATaa                                                                     >  2:3313893/1‑140 (MQ=255)
                                                                      aaCCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATTCGTTTACCTCTTCTTAAGCTGTTTGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTTCCCGCGGGCTGTATTCTAATGa                                                                  >  2:521716/1‑140 (MQ=255)
                                                                        ccTTTCATCAGTGCCGGCGTTTTTGTCGCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTTGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAg                                                                >  2:1259393/1‑140 (MQ=255)
                                                                        ccTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAg                                                                <  2:2759061/140‑1 (MQ=255)
                                                                        ccTTTCATCAGTGCCGGCGTTTTTCTCGCTTATCTTTTCGAATTCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAg                                                                >  2:4172632/1‑140 (MQ=255)
                                                                        ccTTTCATCAGTGCCGGCGTGTTTGTGTCTTATCGTTTAGAATGCGGTTACCTCGTCTTCAGCGGTTGGTGGAGTGGCTGGCGCTGCCCTGGCTGAAACCCTTCGCGGGGGATTTGCCCGCGGGGGGTATTCTAATTAAg                                                                >  1:1725511/1‑140 (MQ=255)
                                                                          tttCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGcc                                                              <  2:2874718/140‑1 (MQ=255)
                                                                               tcaGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAAt                                                         <  1:2235246/140‑1 (MQ=255)
                                                                                   tGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGa                                                     >  2:3225836/1‑140 (MQ=255)
                                                                                   tGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGa                                                     <  1:3880553/140‑1 (MQ=255)
                                                                                   tGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGGTCGCGGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGa                                                     >  2:2020763/1‑140 (MQ=255)
                                                                                     ccGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAAc                                                   >  2:973371/1‑140 (MQ=255)
                                                                                        gCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGc                                                <  2:1771488/140‑1 (MQ=255)
                                                                                        gCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGc                                                >  2:3619858/1‑140 (MQ=255)
                                                                                              ttCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGAt                                          <  2:883403/140‑1 (MQ=255)
                                                                                                             tCGAATGCGTTTACCTCTTCGTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCggcggc                           <  2:3628993/140‑1 (MQ=255)
                                                                                                               gAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCaa                         <  2:1205384/140‑1 (MQ=255)
                                                                                                                 aTGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCaaaa                       <  1:622609/140‑1 (MQ=255)
                                                                                                                 aTGCGTTTACCTCTTCTTCAGCTGGTGGAGGATTCGCTGGCGCTGCCCTGGCTGTAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCaaaa                       <  2:4043980/140‑1 (MQ=255)
                                                                                                                      tttACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGa                  <  2:3979201/140‑1 (MQ=255)
                                                                                                                       ttACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGAt                 <  1:390317/140‑1 (MQ=255)
                                                                                                                         aCCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGTCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCa               <  1:3969426/140‑1 (MQ=255)
                                                                                                                         aCCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCa               <  1:1264776/140‑1 (MQ=255)
                                                                                                                         aCCTCTTCTTCAGCTGGTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCa               <  1:208412/140‑1 (MQ=255)
                                                                                                                              ttcttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTg          >  2:1146627/1‑140 (MQ=255)
                                                                                                                              ttcttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTg          >  2:1672358/1‑140 (MQ=255)
                                                                                                                              ttcttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTg          >  2:2247377/1‑140 (MQ=255)
                                                                                                                              ttcttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCGGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTg          >  2:1380906/1‑140 (MQ=255)
                                                                                                                               tcttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGt         >  2:326379/1‑140 (MQ=255)
                                                                                                                                cttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCa               >  2:345701/1‑133 (MQ=255)
                                                                                                                                cttcAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTa        >  2:4002042/1‑140 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:1541141/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:1019405/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:1611130/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:1081929/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:3785074/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  1:973371/140‑1 (MQ=255)
                                                                                                                                    aGCTGTTGGAGGATTCGCTGGCGCGGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  2:2521196/140‑1 (MQ=255)
                                                                                                                                    aGCGGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCGAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGc    <  2:2737954/140‑1 (MQ=255)
                                                                                                                                     gCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCa   <  2:1924138/140‑1 (MQ=255)
                                                                                                                                      cTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCaa  <  1:3371344/140‑1 (MQ=255)
                                                                                                                                      cTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTAGTCGAATGCAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCaa  <  2:2217487/140‑1 (MQ=255)
                                                                                                                                      cTGTTGGAGGATTAGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCaa  <  1:361683/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
AGTAACAGAGTTTGTCGTGCCTCTTATGGCAGGGGCATACGTGGGAGCCGGTCTCCTCATTGTTCTGATGAACCTTTCATCAGTGCCGGCGTTTTTCTCTCTTATCTTTTCGAATGCGTTTACCTCTTCTTCAGCTGTTGGAGGATTCGCTGGCGCTGCCCTGGCTGAAACCGTTCGCTGGGGATTTGCCCGCGGGCTGTATTCTAATGAAGCCGGAATGGGAACCGCCCCGATTGCACATGCGGCGGCAAAAACGGATCACCCTGTAAGGCAA  >  NZ_CP034943/822095‑822368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: