Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 8.0% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA 8.0% 724.3 / 7.4 298Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (173/88);  new base A (3/34);  total (176/122)
Fisher's exact test for biased strand distribution p-value = 6.78e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

GTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316125‑316401
                                                                                                                                         |                                                                                                                                           
gtgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaa                                                                                                                                           >  2:3679500/1‑140 (MQ=255)
gtgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCaa                                                                                                                                           >  2:261528/1‑140 (MQ=255)
     aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAACCAAACAAATAAAGCATTTCAAATGCt                                                                                                                                      >  2:766644/1‑140 (MQ=255)
        ttACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGc                                                                                                                                   >  1:340360/1‑140 (MQ=255)
              aaGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGACAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACgg                                                                                                                             >  1:3537585/1‑140 (MQ=255)
              aaGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACgg                                                                                                                             >  2:904508/1‑140 (MQ=255)
               attgtTACGGTTATAAGTATTCTATAATATTGAGGGAAAATATGACTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGa                                                                                                                            <  2:2459193/138‑1 (MQ=255)
               actgtTACGGTTATAAGGCTTGTTTAATATTGAGGGAAACTATGAGTGTAGATGAGACACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGa                                                                                                                            <  2:4031354/138‑1 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGCAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGACAACGGaa                                                                                                                           >  1:2699294/1‑140 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                           >  1:117240/1‑140 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                           >  2:3216281/1‑140 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGaa                                                                                                                           >  1:96309/1‑140 (MQ=255)
                gtgtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCGTGCCACCGGaa                                                                                                                           >  2:2870487/1‑140 (MQ=255)
                  gtTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAg                                                                                                                         >  1:3172884/1‑140 (MQ=255)
                    tACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGAc                                                                                                                       >  2:3948511/1‑140 (MQ=255)
                     aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  2:612475/1‑140 (MQ=255)
                     aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  1:810974/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTCGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCCACGGAAAGACaaaaaaa                                                                                                                >  1:3214745/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCCAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:2197177/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:3982871/1‑140 (MQ=255)
                           ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:3364782/1‑140 (MQ=255)
                             aaGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAaga                                                                                                              <  2:3471923/140‑1 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaa                                                                                                                     <  2:157686/127‑1 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:2281807/1‑140 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:3082584/1‑140 (MQ=255)
                                   tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:1892178/1‑140 (MQ=255)
                                     tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:1550665/1‑140 (MQ=255)
                                         atatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGc                                                                                                  >  1:179027/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGTGAAACAAACAAAGAAAGCAGATCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:1914387/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAACGACACAAAAAGAGATCCGGAAAGCGACCgg                                                                                            >  2:3388593/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:3542280/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:3827935/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:2258951/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:54143/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGACAACGGAAAGACAAATAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:3774779/1‑140 (MQ=255)
                                               aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGACGAGAAAGTATCTATAATTTTTGGGAAACCAACAAAGAAAGCAGGTCAAATGCGTGCCAACGGAAAGTCAAAATAAGAGCTCCTGAAAGCGGCCgg                                                                                            >  2:1141739/1‑140 (MQ=255)
                                                   aaaaTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCa                                                                                        >  1:634903/1‑140 (MQ=255)
                                                    aaaTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCaa                                                                                       >  2:3626528/1‑140 (MQ=255)
                                                       tatGCGTGTAGATCACAACCAAATTAAGATAAAAGTCCAGAAAGTATCTAAAATTTTGGGGAAACAAACAAACAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCg                                                                                    <  1:2167108/140‑1 (MQ=255)
                                                       tatGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAAAAAAGAACGCAGTTAAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCg                                                                                    <  2:4000431/140‑1 (MQ=255)
                                                        atGATTTTAGATTATAAACCAATTAATATAAAAGTCGAGAAATTAACTAAAATTTTTGGCAAAAAAAAAAAGAAAGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAACAAATCCTCAAAGCGACCGGATCAACCGt                                                                                   <  2:1045735/140‑1 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATCAGAGTCGAGACATTATCTAAATTTTGTGGGCACCAAACAAAGAACGACGGTCAAATGCTTGGCAACGGAAAGACAAAAAGAGCGAGCGTGACAGCGAGCGTCTCACCCGTTgggg                                                                              >  1:3730350/1‑140 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACACAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:1872519/1‑140 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:2325105/1‑140 (MQ=255)
                                                             tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:1394787/1‑140 (MQ=255)
                                                               tAGATGAGAAACCAATTAAGATAAAATTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  2:3927073/1‑140 (MQ=255)
                                                               tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTCTCTAAGATTTTTGGGAAACAAACAAAGAAGGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCAACCGGATCAACCGTTGGGGtt                                                                            >  1:2083001/1‑139 (MQ=255)
                                                                  aTGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAAt                                                                         >  1:494630/1‑140 (MQ=255)
                                                                     agaAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCaa                                                                      >  2:3163464/1‑140 (MQ=255)
                                                                       acacaatttAAGATAAACGCCGAGAAAGTATATAAAATTTTGGGGAAACAAACAAATAAAGCCGTTAAAAGGCTTGCCACCCGAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  1:4142379/133‑1 (MQ=255)
                                                                       aaaaCAATTAAGATAACAGTCGAGAAAGTATCTAAAATTTTTGGCAAAAAAAAAAAGAAAGCAGTTAAAATGCTGGCCACCGCAAAGAAAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  1:904508/140‑1 (MQ=255)
                                                                       aaaCCATTTAAGATAAAAGTCTAGAAAGTATCTAAAATTTTTGGCAAACAAACAAACACAGCAGTTCAAATGCTGGCCAACGCAAACAAAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  2:1614370/140‑1 (MQ=255)
                                                                       aaaCCAATTAAGATAAACGTCTACAAAGTAACTAAAATTTTTGGCAAACAAACAAAGAAAGCAGTACAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    <  2:1777181/140‑1 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTCAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:1253460/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:2532656/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:1710061/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:620832/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:426846/1‑140 (MQ=255)
                                                                       aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  1:1610219/1‑140 (MQ=255)
                                                                            aaTTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCATGAAAGCGACCGGATCAACCGTGGGGGTAAATCAAGCAGAtt                                                               >  2:2800682/1‑140 (MQ=255)
                                                                             aTTAAGACAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGATCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAttt                                                              >  2:2065300/1‑140 (MQ=255)
                                                                               tAAGATAAAAGTCGAGAAATTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:4167261/140‑1 (MQ=255)
                                                                               tAACATAAACGCCGAGAAAGAACCTAAAATTTTGGGCAAACAAAAAAAGACCGCATTCCAACTGGTTGCCAACGGAAACAAAAAAAAACCCAGCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:3442742/140‑1 (MQ=255)
                                                                                 ataTAAACGTCGAGAAAGTATATAAAATTTTTGGTAAACAAAAAAATAAAGCAGTACAAATGCTTGCCAACGGAAAGACAAAAAAAGAGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:952616/138‑1 (MQ=255)
                                                                                 acacaaaaGCCTAGAATGTATCTAAAATTTTGCGTAAACAAAAAAAGAAAGCTGTTCAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTTAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:3884637/136‑1 (MQ=255)
                                                                                 aGAAAAAAGACGACAAAGTATATAAAATTTTTGGGAAACAAACAAAGAAACCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGACACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:848643/140‑1 (MQ=255)
                                                                                  taaaaacGTCGACAAACTATATAAAATTTTTGGCAAACAAACAAACAAAGCAGTACAAATGCTTGCCACCGCAACCAAAAAAAAAGACACCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATg                                                         <  1:3597406/137‑1 (MQ=255)
                                                                                      aaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAATCGACCGGATCAACCGTTGGGGTAAATCACGCAGATTTTGATGtata                                                     >  2:238756/1‑140 (MQ=255)
                                                                                        agtctttaaaTTATATAAAATTTTTGTGAAACAAACAAAGAAACCAGTTAAAAGGGTTGCCAACGGAAACACAAAAAAAGAGTTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATatg                                                   <  1:4025495/133‑1 (MQ=255)
                                                                                            tataaaTTATATAAAATTTTGGGCAAAAAAAAAAACAAACAATTAAAAAGGCTGGCAACCGCAAACACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               <  2:2334716/137‑1 (MQ=255)
                                                                                              taacttATCTAAATTTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGAGAGCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:675936/135‑1 (MQ=255)
                                                                                              caatttATCAAAATTTTTTGGCAAAAAAAAAAATAACGCTTTTAAAATGCTTCCCAACGTAAACAAAAAAAAAGATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:1430991/135‑1 (MQ=255)
                                                                                              caaaGTATATAAATTTTTTGGCAAAAAAAAAAAGAAAGCAGTAAAAATGGTTCCAAACGCAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTg                                             <  1:769564/139‑1 (MQ=255)
                                                                                                aaGTATCTAAATTTTTTGGGAAAAAAAAAAAGAAAGAAGTTCAATTGCTTGCCAACGTAAAGACAAAAAAAGAGATCATTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTgag                                           <  2:1925447/140‑1 (MQ=255)
                                                                                                    atatAAAATTTTTGGCAAACAAACAAAGAAAGCAGTAAAATTGCTGCCCAACGTAAAGAAAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  1:2429652/137‑1 (MQ=255)
                                                                                                        aaaaTTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAATGAGATCCTGACAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGCTGTTGAGGTCTGTGAGGGTGAGATATTTgg                                   >  2:3608415/1‑139 (MQ=255)
                                                                                                            tttttGGCAAAAAAAAAAACAACCCATTTCAAATGCTTCCCAACGCAAACAAAAAAAAAGACATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatc                               <  1:2606911/140‑1 (MQ=255)
                                                                                                             ttttCGCAAACAAACAACGAACCCAGTACAAATGCTTGCCAACGCAAACACAACAAAAGAGCGCCTGAAAGCGACCGGATCAACCGTGGGGGTAAATCACGCAGATTTTGATGTATATGATGGTGAGATATTTGtcatca                              <  1:3383132/140‑1 (MQ=255)
                                                                                                              tttGTGAAACAAACACACAAAGACGTACACTGGCTGGCCAACGCAAACACAAAAAAAGAGTGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:3668635/140‑1 (MQ=255)
                                                                                                              ttggggAAACAAACAAAGAACGCCGTAAAAAGGCTTGCAAAGGGAACGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:3082584/137‑1 (MQ=255)
                                                                                                              tgtGGCAAACAAACAAACAAAGCCGTTCAAATGCTTCCCAACGCAAAGACAAAAAAAGACATCATTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:3626528/138‑1 (MQ=255)
                                                                                                                tGGGCAAAAAACAAATAAAGCAGTCAAACGGCTTGCCACCGGTAACCCAAACAATGCGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTACATCAAGCAGTTTTTGATGTATATGATGGTGAGTTATTTGTCATCATgg                           <  2:332231/140‑1 (MQ=255)
                                                                                                                tGGCAAACAAACAAAGAAACCAGTTAAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:3515584/140‑1 (MQ=255)
                                                                                                                tGGCAAACAAAAAACGAACGCAGTTCAATTGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:1746166/140‑1 (MQ=255)
                                                                                                                tGGCAAAAAAAAAAAGAAAGCATTTAAAATGCTTGCCAACGTAAACACAAAAAAACAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:2835433/140‑1 (MQ=255)
                                                                                                                ggggAAACAAACAAACACAGCAGTACAAATGCGTGCCACCGCAAAGCCAAAAAAAGACCTCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:1932551/139‑1 (MQ=255)
                                                                                                                  gtaaaaaaaaaaACAAACCAGTACAATTGCTTCCCAACGCAAACAAAAAAAAACAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:3308003/138‑1 (MQ=255)
                                                                                                                  gaaaacaaacaaacAACGATCTTCAAATGCTGCCCAACGCAAAGACAAAAAAACAGAGCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:1643235/138‑1 (MQ=255)
                                                                                                                           caaaGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                <  2:3364782/140‑1 (MQ=255)
                                                                                                                           caaaGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAggg                <  2:3182818/140‑1 (MQ=255)
                                                                                                                                 aagCAGTTAAATTGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt          <  1:736306/140‑1 (MQ=255)
                                                                                                                                     atttCAATTGCTTGCAAACTCAAATACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  2:2511569/138‑1 (MQ=255)
                                                                                                                                     aGTTCAAATGCTTGCCAACGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  2:683654/140‑1 (MQ=255)
                                                                                                                                     aGTTCAAATGCTTCCCAACGGAAACAAAAAAAAAGAGATCCCGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  1:289742/140‑1 (MQ=255)
                                                                                                                                     aGTTCAAATGCTGGCCACCGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  1:1001248/140‑1 (MQ=255)
                                                                                                                                      tttCAAATTCTTGCCAACGCAAAGACAAAAAAAGATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:1976117/139‑1 (MQ=255)
                                                                                                                                      gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGGTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     >  2:254531/1‑140 (MQ=255)
                                                                                                                                         cAAATGTTTGCAAACGTAAACAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc  <  2:1236635/140‑1 (MQ=255)
                                                                                                                                         cAAATGGTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc  <  2:1445629/140‑1 (MQ=255)
                                                                                                                                         cAAATGCTTGCCACCGGAACCACACAAAAAGAGCTCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAc  <  2:2419987/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316125‑316401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: