Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,151,967 T→G 12.6% K79T (AAA→ACA)  EO946_RS05920 ← GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,151,9670TG12.6% 336.6 / 14.5 156K79T (AAA→ACA) EO946_RS05920GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (50/81);  new base G (23/2);  total (73/83)
Fisher's exact test for biased strand distribution p-value = 3.77e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

TCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTT  >  NZ_CP034943/1151828‑1152105
                                                                                                                                           |                                                                                                                                          
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                            <  1:2237268/140‑1 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                            >  1:1097146/1‑140 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCAGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                            >  1:2306446/1‑140 (MQ=255)
tCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCGGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGtt                                                                                                                                            <  1:2882957/140‑1 (MQ=255)
  ggACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTg                                                                                                                                          <  2:3471668/140‑1 (MQ=255)
    aCTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTa                                                                                                                                        <  2:2186718/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCTCTTTCTCATAAAGCGGCAACCCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCAGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  1:3749351/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAACCCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  2:1720891/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  1:1714681/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    >  1:2207583/1‑140 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  1:3659511/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  1:3187507/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  1:279024/140‑1 (MQ=255)
        aaCCCGCTGTTTGAAAACCCGCTGTCTCATAACGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATCGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCt                                                                                                                                    <  2:189990/140‑1 (MQ=255)
            ctgtGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCATGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                <  2:898558/137‑1 (MQ=255)
            cGCTGTTTGACAACCCGCTTTCTCAGAACGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                <  2:2380937/140‑1 (MQ=255)
            cGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCTAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                <  2:3004195/140‑1 (MQ=255)
            cGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                <  2:1444542/140‑1 (MQ=255)
            cGCTGTTTGAAAACCCGCTCTCTCAGAACGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGt                                                                                                                                <  2:2906771/140‑1 (MQ=255)
              cTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCt                                                                                                                              >  2:957221/1‑140 (MQ=255)
                  ttGAAAACCCGCTTTATCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTTATAGCGGTGCTTTCAGCCGTTGGGCTGATACACGCGGTCCCCATCCGCCGACCGAGTCCGAGCCGTTTTTAGTCTTTGTCTAGGa                                                                                                                          >  2:2369828/1‑140 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                      <  2:106607/140‑1 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                      <  1:2756521/140‑1 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                      <  1:3907704/140‑1 (MQ=255)
                      aaaCCCGCTTTCTCATAAAGCGGCAAACCTTCTTGTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGAc                                                                                                                      <  2:2665273/140‑1 (MQ=255)
                         cccGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAg                                                                                                                   <  1:395840/140‑1 (MQ=255)
                         agacagttCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAg                                                                                                                   <  1:3358067/134‑1 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCGAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTc                                                                                                                 <  2:672350/140‑1 (MQ=255)
                           cGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTc                                                                                                                 >  2:2314534/1‑140 (MQ=255)
                               ttCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGAt                                                                                                             <  1:2567288/140‑1 (MQ=255)
                               ttCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGAt                                                                                                             <  1:858297/140‑1 (MQ=255)
                                       aaGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCaa                                                                                                     <  1:3165852/140‑1 (MQ=255)
                                       aaGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCaa                                                                                                     <  1:3523714/140‑1 (MQ=255)
                                          cGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAg                                                                                                                   >  2:1336295/1‑123 (MQ=255)
                                          cGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAg                                                                                                                   <  1:1336295/123‑1 (MQ=255)
                                          cGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTAAGATGACGAg                                                                                                                   >  2:2818759/1‑123 (MQ=255)
                                          cGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTGGTATTGTTTTTCTACGATGACGCGTCCGCTAGAGGcggtct                                                                                                  >  2:3358067/1‑135 (MQ=255)
                                            gCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTTTATTATTTTTCTAAGATTAACAGTCCGATAAAGGCaaaaagg                                                                                                >  1:3506948/1‑135 (MQ=255)
                                            gCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAACACgg                                                                                                >  2:260437/1‑140 (MQ=255)
                                            gCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTATACGATGACGAGGCCGATAGAGGCAACACgg                                                                                                >  2:2871229/1‑140 (MQ=255)
                                             cAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGc                                                                                               <  1:819704/140‑1 (MQ=255)
                                                           tgGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTGTTTTTCTACGCAGAAGAGTCCGATAGAGGCAAGAACGCCcaacaaaaaaaaa                                                                                 >  1:96440/1‑139 (MQ=255)
                                                           tgGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATTACGAGGCCGATAGAGGCAAGACGGCCaaacaaaaaacag                                                                                 >  1:340373/1‑136 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACCCGCTTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGc                                                                                <  1:736338/140‑1 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTTTATAAGATGACGAGTCCGATAGAGGCCAGACGGCCAAACAAAAACAAGc                                                                                >  2:690594/1‑140 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTAATTGTCTACGATGACGAGTACGATAGAGGCAAGAACGCCAAACAAAAAAAAGc                                                                                >  2:3228981/1‑140 (MQ=255)
                                                            ggCGCAAAGCCTGATAGCGGTGCGTTCATCCGTTTGGCTGATCCCCGCGTTCCCCATCCGCCGTCCGAGACCGAGCCGTGTGGATTGGTTGTCTACGATGACGCGGCCGAGAGGGGCCAGACGGCCcaacaataactcgc                                                                                >  2:462365/1‑136 (MQ=255)
                                                                 aaaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATg                                                                           <  2:135921/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                          <  1:4236095/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                          <  1:1881743/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                          <  1:3869461/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                          <  1:3816986/140‑1 (MQ=255)
                                                                  aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGAGACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                          <  1:3743330/140‑1 (MQ=255)
                                                                       tGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATTACGAGTCCGATAGAGGCAACACCGCCCAACAAAAACAAGCCGATGCAggga                                                                     >  2:137315/1‑138 (MQ=255)
                                                                              ggTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTTTGTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAACCGATTCACGAaataaatt                                                              >  1:275072/1‑137 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTTTATAAGATGACGAGTCGGATAGAGGCAACACGGCCAAAAAAAAAAAAGACGATGCAGGCAAAAAAGtc                                                             >  1:3444439/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATTACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAACCGATGCAGGAAAAAATTtc                                                             >  2:3744806/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGtc                                                             >  2:2420180/1‑140 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTGTTTTTCTAAGATGACGAGTGCGATAGAGGCAAAACGGCCAAACAAAAAAAAACCCATTCAGGAaaaaattta                                                             >  1:89419/1‑137 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTATTTTTGTAACATTACGAGTCCGCTAGAGGCAACACGGCCAAACAAAAACAAACCGATGCAGGGaaaaattta                                                             >  1:1783509/1‑137 (MQ=255)
                                                                               gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGATTAGTTTTCTAAGATGACGAGGCCGCTAGCTGCAACACCGCCCAACAAAAACAAGCCGATGCCGGAAATAATGtg                                                             >  1:468552/1‑139 (MQ=255)
                                                                                   tttCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGTTTGTTTTTGTAAGATGACGAGTCCGATATAGGCAAGACGGCCAAAAAAAAAAAATCCCATGCAGGAAAAAATGTATCcg                                                         >  1:3330723/1‑140 (MQ=255)
                                                                                   tttCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGATTATTTTTCTTCGGTGACGCGTGCGCTATAGGCAAGAAGGCCAAACAAAAAAAAGCCGATGCAGGAAAAAATGtgggcg                                                         >  1:2155362/1‑135 (MQ=255)
                                                                                     tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                       <  1:1379535/140‑1 (MQ=255)
                                                                                       aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTGTACGATGACGAGTCCGAAATAGGCAAGACGGCCAAAAAAAAAAAAGCCGATGCAGGAAATAAAGTATACGCGGa                                                     >  2:3208730/1‑140 (MQ=255)
                                                                                        tacGTTTGGCTGATACACGCGTTCACCCTCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGACGAAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGAc                                                    <  1:1191544/138‑1 (MQ=255)
                                                                                                gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                            <  1:883477/140‑1 (MQ=255)
                                                                                                gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                            <  1:1429003/140‑1 (MQ=255)
                                                                                                      acacGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTGTAAGATGACGAGTCCGAGAGAGGCAAGACGGCCCAACAAAAAAAACCCGATGCAGGAAATAATGTCTCCGCGGACAATTTTATAACCGa                                      >  2:2007429/1‑140 (MQ=255)
                                                                                                      acacGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACCATGACGAGTCCGATAGAGGCAACACGGCCAAACAAAAACAACCCGATGCACGAAATAAAGTCTCCGCGGACATTTTGATAAACGa                                      >  2:2681374/1‑140 (MQ=255)
                                                                                                          gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACCAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAACCCGATGCAGGAAATAATTTCTCCGCGGACATTTTGATAACCGaaaaa                                  >  2:2223634/1‑140 (MQ=255)
                                                                                                           cgTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAg                                 <  2:2370251/140‑1 (MQ=255)
                                                                                                           cgTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAg                                 <  2:799141/140‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACTATGACGAGTCCGATAGAGGAAAGACGGCCAAAAAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAgcgc                              <  1:2171111/140‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcg                                                         <  1:2997336/113‑1 (MQ=255)
                                                                                                              tCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcg                                                         >  2:2997336/1‑113 (MQ=255)
                                                                                                               cacaATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTTTAAACGCAGACGAGTCCGCTAGATGCCACACGGCCAAAAAAAAACAACCCCATGCAGGAAATAATGTCTCCGCGGACAGTTGTATAACCGAAAAATCGcc                             >  2:2755111/1‑140 (MQ=255)
                                                                                                                   ctCCGCCGGCCGCGACCGAGCCGTTTTTATTCTTTGTCTACGATGACGAGGCCGATCGAGGCAAGACGGCCAAACAAAAACCAGCGGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTg                         <  2:2405592/139‑1 (MQ=255)
                                                                                                                   cggcgccgcCCGAGCCCGAGCCGTGTCTCTTCGTGCTCTCCGATGACGAGTCCGCTACCGGCAAGACGGCCAAACAAAAACAAGCCGATCCAGGAAATAATGGCTCCGCGGACATTTTGATACCCGAAAAAGCGCCCCTg                         <  2:2404972/137‑1 (MQ=255)
                                                                                                                   aTCGGCCGTACGAGAACTAGCCGTTTGTTTTCTTTTTATACGATGACGAGTCCTATAGAGTAAAGACGGCCAAACAAAAACAAGCCGATGCAGTAAATAAGTTCTCCGCGGAAATTTTGATAACCGAAAAAGCGCCCCTg                         <  1:2518824/140‑1 (MQ=255)
                                                                                                                   aTCCGCCGTCCTAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCAAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTg                         <  1:3228981/140‑1 (MQ=255)
                                                                                                                    tCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGt                        <  2:275072/140‑1 (MQ=255)
                                                                                                                    tCCGCCGTCCGAGACCGAGCCGTGTGTATTCGTTTTCTAACATTACCAGTACGATAGAGGCAAGAAGGCCAAACAAAAACAAGCCGATGCAGGAAAAAATTTATACGCGGACCGTTTGATAAACGAAAAACccccccatt                        >  1:2117922/1‑137 (MQ=255)
                                                                                                                            ccGAGACCGAGCCGTTTGTATTGTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTTATAAACGAAAAAGCGCCCCTGTTGGACAAg                >  2:1072231/1‑140 (MQ=255)
                                                                                                                            ccGAGACCGAGCCGTTTGTATTCTTTGTCTACGAAGTCGAGTACGATAGAGGCCAGACCGCCAAAAAAAAACAACCCGATGCAGGAAATAATGTCTCCCCGGCCAATTTGATAACCCAAAAAACCCCCCAGTTTGaaaac                >  2:3872880/1‑139 (MQ=255)
                                                                                                                              gagaCCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCa              <  2:89419/140‑1 (MQ=255)
                                                                                                                              gagaCCGAGCCGTTTGTATTATTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCa              <  2:2025338/140‑1 (MQ=255)
                                                                                                                              gagaCCGAGCCGTTTGGATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCATGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCa              <  2:256099/140‑1 (MQ=255)
                                                                                                                                   cGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcc         <  2:2510125/140‑1 (MQ=255)
                                                                                                                                   cGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcc         <  2:3621180/140‑1 (MQ=255)
                                                                                                                                   cGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcc         <  2:2458334/140‑1 (MQ=255)
                                                                                                                                   cGAGCCGTTTGTATTCTTTGTCGACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGcc         <  2:1294980/140‑1 (MQ=255)
                                                                                                                                     aGCCGTTTTTGTTCTTTGTCTACGATGACGAGTGCGAAATAGGCAAGACCGCCAAACAAAAACAACCCGATGCAGGAAATAATGTGTCCGCGGACATTTTGATAACCGAAAAATCGCCCCGGGTGGAAAagccaagccac       >  2:506283/1‑139 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTTTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAACCCGATGCAGGAAAAAAAGTCACCGCGGACAATTTGATAACCGAAAAAGCGCCCCGGTTGGCCAAGCAATGCCAg       >  2:3216130/1‑140 (MQ=255)
                                                                                                                                     aGCCGTTTGTATTCTTTGTCTAAGATTAAGAGTCCGATAGAGGCAAAACGGCCAAAAAAAAAAAAGCCGATGCAAGAAAAAAAGTCTGCGCGGACCATTTTATAACCCAAAAAGCGCCCCTGTTGGACAAGCAAAGCCAg       >  2:1529449/1‑140 (MQ=255)
                                                                                                                                      gCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGTCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGc      <  2:3972767/140‑1 (MQ=255)
                                                                                                                                          tttGTATTCTTTTTCTACGATTAAGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAACCCCATGCACGAAATAATGTCTCCGCGCACAATTTTATAACCGAAAAAGCGCCCCTGTTTGACAAGCAAAGCCACCTCtt  >  2:454278/1‑140 (MQ=255)
                                                                                                                                          tttGTATTCTTTTTCTAAGATTACGAGTCCGATAGACGCAACACCGCCAAACAAAAACAACCCGATGCACGAAATAATATCTCCGCGGACATATTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCACtt  >  2:2880488/1‑140 (MQ=255)
                                                                                                                                          tttGTATTCTTTGTCTACGATGACGCGTCCGATAGAGGCAATACGGCCAAAAAAAAAAAATCCGATGCCGGAAAAAATGTCGCCGCGGACCATTTGATAACCGAAAAATCGCCCCGGTTGGGCAACCAATACCACctcgt  >  2:1769105/1‑138 (MQ=255)
                                                                                                                                          tttGTATTCTTTGTCTAAGATTACGAGTCCGATAGAGGCAACACGGCCAAACAAAAACAAACCGATGCAGGAAAAAAATTCTCCGCGGACAATTTTATAAACCAAAAAGCGCCCCGGTTGGACAAGCAATGCCAGctcat  >  2:1796986/1‑138 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCGGACTGAACCCGCTGTTTGAAAACCCGCTTTCTCATAAAGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTT  >  NZ_CP034943/1151828‑1152105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: