Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,450,016 G→C 5.1% P59P (CCG→CCC ccpC → transcriptional regulator CcpC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,450,0160GC 5.1% 610.1 / 16.4 185P59P (CCG→CCCccpCtranscriptional regulator CcpC
Reads supporting (aligned to +/- strand):  ref base G (94/81);  new base C (4/5);  total (99/86)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 198 total aligned reads displayed.

GAGGAGTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGCATTGGC  >  NZ_CP034943/1449879‑1450155
                                                                                                                                         |                                                                                                                                           
gaggagTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACgccggc                                                                                                                                           >  1:1177000/1‑140 (MQ=255)
gaggagTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACgccggc                                                                                                                                           >  1:912223/1‑140 (MQ=255)
gaggagTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACgccggc                                                                                                                                           >  1:2208918/1‑140 (MQ=255)
 aggagTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACgccggcc                                                                                                                                          >  1:2616957/1‑140 (MQ=255)
      ttACATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCATCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAACGGGTTAACGGTAACGCCGGCCGGTga                                                                                                                                     <  2:2249937/140‑1 (MQ=255)
      ttAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTga                                                                                                                                     <  2:350380/140‑1 (MQ=255)
        aaaTATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGATAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTgaga                                                                                                                                   <  1:4272350/140‑1 (MQ=255)
              gagaAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAATAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGCCCGGTGAGAAAATCa                                                                                                                             >  2:1820465/1‑140 (MQ=255)
              gagaAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCa                                                                                                                             >  2:941052/1‑140 (MQ=255)
              gagaAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCa                                                                                                                             >  2:4249195/1‑140 (MQ=255)
              gagaAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCa                                                                                                                             <  2:1464127/140‑1 (MQ=255)
                gaAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCAtt                                                                                                                           >  2:986605/1‑140 (MQ=255)
                   aGGCGGCAGAACGGCTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAATGGCGTGGGGAACAAAAATCTTTTTAAGATCGCAAAACGGATTAACGGTAACGCCCGCCGGGGAGACCATCATTCAg                                                                                                                        >  1:2759627/1‑140 (MQ=255)
                   aGGCGGCAGAACGGCTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGTAACAAAAATCTTTTTAAGATCTCAAAAAGTATTAAAGGTAACGCCAGCCGGTGAGAAAATCATACAg                                                                                                                        >  1:850802/1‑140 (MQ=255)
                   aGGCGGCAGAACGGCTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGAACAAAAATCTTTTTAAGATCTCAAAAAGGATTAACGGTAACGCCCGCCGGTGAGAAAATCATTCAg                                                                                                                        >  1:3126554/1‑140 (MQ=255)
                        gcggAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGc                                                                                                                   >  2:1605479/1‑140 (MQ=255)
                          ggAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTACCGCCGGCCGGTGAGAAAATCATTCAGTTTGCGa                                                                                                                 <  1:2959615/140‑1 (MQ=255)
                                aCTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAatgatg                                                                                                           >  1:2044620/1‑140 (MQ=255)
                                 cTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  1:2132553/140‑1 (MQ=255)
                                 cTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  2:377858/140‑1 (MQ=255)
                                 cTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  1:652806/140‑1 (MQ=255)
                                 cTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACACCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  1:2460433/140‑1 (MQ=255)
                                   ttttGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  1:3698186/138‑1 (MQ=255)
                                   ttttGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          >  2:3698186/1‑138 (MQ=255)
                                      tGTATCTCAGCCGGATTTATCTCAGAGCGTACAAACCATGGAAAAGGCGTCGGGCACAAGAATCTTTTCAAGATCCCACAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGt                                                                                                     <  2:2533491/140‑1 (MQ=255)
                                           ctcAGCCGGCTTTATCTCAGCGCTTACACACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTagag                                                                                                <  1:2849281/140‑1 (MQ=255)
                                           ctcAGCCGGCTTTATCTCAGCGCTTACAACCCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTagag                                                                                                <  1:1813313/140‑1 (MQ=255)
                                           ctcAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACTGTAACGCCGGCCGGTGAGAAAATCATTAAGTTTGCGAATTATGTGACGGTagag                                                                                                >  1:2680852/1‑140 (MQ=255)
                                           ctcAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTagag                                                                                                <  1:1345643/140‑1 (MQ=255)
                                                ccGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          <  2:887636/125‑1 (MQ=255)
                                                ccGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgt                                                                                                          >  1:887636/1‑125 (MQ=255)
                                                ccGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGa                                                                                           <  1:2297831/140‑1 (MQ=255)
                                                ccGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGa                                                                                           <  1:1562541/140‑1 (MQ=255)
                                                      ttATCTCAGCGCTTACACACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAAt                                                                                     <  1:3878620/140‑1 (MQ=255)
                                                        aTCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATaa                                                                                   >  2:3818197/1‑140 (MQ=255)
                                                          ctcAGCGCTTACAAACCATCGAAAACGCGTGGGTAACTAAATTCTTTTTTAGATCCCATAAATTGTTAAGGGTATTGGCGGGCTGTGTGATATTATTACATTTTTCTAATTTTTTTATGCTAGAGCAGGAAAGAATAaga                                                                                 <  2:2057217/140‑1 (MQ=255)
                                                          ctcAGCGATTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAaga                                                                                 <  2:2382408/140‑1 (MQ=255)
                                                          ccccgcccTTACACACCACCGCAAAGGCGTCGGGTAAAAAACTCTTTTGAACATCCCATAACTTGATATCGGTATCCCTGGCCTGTGTGAAAATAATTAAGTTTGCGAATGATGTTACGATAGAGCAGGAAAGAATAaga                                                                                 <  2:2192396/133‑1 (MQ=255)
                                                            cAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAgaga                                                                               <  1:264236/140‑1 (MQ=255)
                                                             aGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGAtgtg                                                                                                         <  1:3793840/113‑1 (MQ=255)
                                                              gcgcTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGaaa                                                                             >  1:4121881/1‑140 (MQ=255)
                                                               cgcTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGa                                                                                                                 >  2:2486692/1‑103 (MQ=255)
                                                               cgcTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGa                                                                                                                 >  2:923532/1‑103 (MQ=255)
                                                               cgcTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGa                                                                                                                 <  1:916265/103‑1 (MQ=255)
                                                                   tACAAACCATTGACAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATAtt                                                                        >  2:3537070/1‑140 (MQ=255)
                                                                   tACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCCGGAAAGAATAAGAGAAACTAtt                                                                        >  1:3656523/1‑140 (MQ=255)
                                                                   tACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAAAGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATAtt                                                                        >  1:433894/1‑140 (MQ=255)
                                                                    aCAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATtg                                                                       >  1:1218416/1‑140 (MQ=255)
                                                                    aCAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATtg                                                                       >  1:1971648/1‑140 (MQ=255)
                                                                      aaaaCATTTAAAAGGAGTGGGTCACAAAAATCTTTTTAAGATCCCAAAAAGTGTTAACGGTAACTCAGTCCGGTTATAAAATCATTAAGTTTTCGAATTATGTGACGTTTGAGTATTAAAGAATTAGAGTAAATATtgat                                                                     <  2:2427650/140‑1 (MQ=255)
                                                                      aaaCCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATtgat                                                                     <  1:171965/140‑1 (MQ=255)
                                                                      aaaCCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATtgat                                                                     <  1:2613783/140‑1 (MQ=255)
                                                                            ttcaaaaGGCGTGGGCCAAAAAAATAATATAAAGATCACAAAAACGGTTAAAGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  2:1071058/4‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAATGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               <  2:823306/140‑1 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGACAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:3193301/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGACAATATTGATGAGCtt                                                               >  1:1270012/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:962633/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:2305285/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  2:2972818/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:2078264/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:3889282/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  2:741882/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCtt                                                               >  1:1182652/1‑140 (MQ=255)
                                                                            ttGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATGGATGAGCtt                                                               <  2:2620358/140‑1 (MQ=255)
                                                                              gataaGGCGCGGGCCACAAAAATCTTTTAAAGATCCCAAAAACGGTTAACGGTCACGCCGGCCGGTGAGAAAATCCTTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAACTATTGATGAGCTTGa                                                             <  2:4032424/137‑1 (MQ=255)
                                                                               aaaaGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTACCGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCCGGAAAGAATAAGAGAAAATATTGATGAGCTTGaa                                                            >  2:1414038/1‑140 (MQ=255)
                                                                                   ggCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTg                                                        <  2:189388/140‑1 (MQ=255)
                                                                                    gCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGCTGAGCTTGAAGGTGa                                                       >  1:3565359/1‑140 (MQ=255)
                                                                                    gCGTGGGGCACAAAAATCTTTTGAAGATCCCAACAAGGGTTCACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGACTGCTGTGACGTTAGAGCAGGACAGACGAAGAGCAAATATTGATGAGCTTGAAGGTGa                                                       >  2:1734468/1‑140 (MQ=255)
                                                                                       tGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGaa                                                                                                                >  1:2282058/1‑80 (MQ=255)
                                                                                         gggCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTc                                                  >  2:609337/1‑140 (MQ=255)
                                                                                          ggCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCa                                                 <  2:2044620/140‑1 (MQ=255)
                                                                                          ggCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCa                                                 <  2:3777152/140‑1 (MQ=255)
                                                                                            cacaAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACg                                               <  1:3339373/140‑1 (MQ=255)
                                                                                             acaAAAATCTTTTTAAGATCCCAAACAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACgg                                              <  1:3851287/140‑1 (MQ=255)
                                                                                              caAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGACAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGc                                             >  2:2553881/1‑140 (MQ=255)
                                                                                              caAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGACAATATTGATGAGCTTGAAGGTGAAATTCACGGc                                             >  2:584163/1‑140 (MQ=255)
                                                                                              caAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGc                                             >  2:2326320/1‑140 (MQ=255)
                                                                                               aaaaaTCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGca                                            <  2:341756/140‑1 (MQ=255)
                                                                                               aaaaaTCTTTTTAAGATCCCAAAAAGGGTTAACGCTACCGCCGGCCGGTGAGAAAATCCTTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAACTATTGATGAGCTTGAAGGTGAAATTCACGGca                                            <  2:3980061/140‑1 (MQ=255)
                                                                                                   aTCTTTTTAAGATCCCAAACAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAACATATTGATGAGCTTGAAGGTGAAATTCACGGCACACt                                        <  1:569606/140‑1 (MQ=255)
                                                                                                   aTCTTTTTAAGATCCCAAACAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACt                                        <  1:2487745/140‑1 (MQ=255)
                                                                                                   aTCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACt                                        <  1:932916/140‑1 (MQ=255)
                                                                                                       ttttAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAg                                    >  2:3247047/1‑140 (MQ=255)
                                                                                                                cccAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTc                           >  1:76596/1‑140 (MQ=255)
                                                                                                                cccAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTGTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTc                           <  2:3582431/140‑1 (MQ=255)
                                                                                                                          attAACGGTAACGCCCGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACATTAGAGCAGGAAAGAATAAGAGAAAATATTGACGAGCTTGAAGGTGAAATTCACGGCACATTGAAGCTTGCCGTCGCCTCCATaa                 <  2:3126554/139‑1 (MQ=255)
                                                                                                                          attAACGGTAACGCCCGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACATTAGAGCAGGAAAGAATAAGAGAAAATATTGACGAGCTTGAAGGTGAAATTCACGGCACATTGAAGCTTGCCGTCGCCTCCATaa                 <  2:4331607/139‑1 (MQ=255)
                                                                                                                          attAACGGTAACGCCCGCCGGTGAGAAAATCATTCAGTGTGCGAATGATGTGACATTAGAGCAGGAAAGAATAAGAGAAAATATTGACGAGCTTGAAGGTGAAATTCACGGCACATTGAAGCTTGCCGTCGCCTCCATaa                 <  2:2759627/139‑1 (MQ=255)
                                                                                                                             aaCGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCg              >  2:2758415/1‑140 (MQ=255)
                                                                                                                             aaCGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGACTCCATAATCg              >  1:4091930/1‑140 (MQ=255)
                                                                                                                              aCGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATTTTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCgg             <  1:3967960/140‑1 (MQ=255)
                                                                                                                              aCGGTAACGCCGGCCGGTGAGAAAATCAGTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCgg             <  1:3406446/140‑1 (MQ=255)
                                                                                                                               cGGTAACGCCGGCCGGTGAGAAAATCATTCTGTTTGCGAATGAGGTGACGTTAGAGCAGGAAAGAATAAGAGAAAAGATTGATGAGCTTGAAGGTGACATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGc            <  1:2183316/140‑1 (MQ=255)
                                                                                                                               cGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGc            <  1:609337/140‑1 (MQ=255)
                                                                                                                                   aaCGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGc        >  1:554118/1‑140 (MQ=255)
                                                                                                                                   aaCGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGc        >  2:833820/1‑140 (MQ=255)
                                                                                                                                   aaCGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGc        >  2:334337/1‑140 (MQ=255)
                                                                                                                                     cGCCGGCCGTATATAAAATCATTCAGTTTTCGAATTATTTGACGTTAGAGCAGTAAAGAATAAGAGAAAATATTTATGAGCTTGAAGGTTAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGCAt      <  1:3402344/140‑1 (MQ=255)
                                                                                                                                        cggccggTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGCATTgg   <  1:1427435/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GAGGAGTTAAATATGAGAAAGGCGGCGGAACGACTTTTTGTATCTCAGCCGGCTTTATCTCAGCGCTTACAAACCATTGAAAAGGCGTGGGGCACAAAAATCTTTTTAAGATCCCAAAAAGGGTTAACGGTAACGCCGGCCGGTGAGAAAATCATTCAGTTTGCGAATGATGTGACGTTAGAGCAGGAAAGAATAAGAGAAAATATTGATGAGCTTGAAGGTGAAATTCACGGCACACTGAAGCTTGCCGTCGCCTCCATAATCGGCCAGCATTGGC  >  NZ_CP034943/1449879‑1450155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: