Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,463,824 G→T 10.1% D44Y (GAT→TAT)  EO946_RS07665 → ABC transporter ATP‑binding protein/permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,463,8240GT10.1% 448.6 / 19.9 188D44Y (GAT→TAT) EO946_RS07665ABC transporter ATP‑binding protein/permease
Reads supporting (aligned to +/- strand):  ref base G (74/91);  new base T (19/4);  total (93/95)
Fisher's exact test for biased strand distribution p-value = 6.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

GGATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGTTTGGAATCAAATCCG  >  NZ_CP034943/1463691‑1463956
                                                                                                                                     |                                                                                                                                    
ggATATGGAGACATTTAAAAGATTAACAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAACTTACGATTGATGaaa                                                                                                                                <  2:1004041/140‑1 (MQ=255)
ggATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGaaa                                                                                                                                <  2:839038/140‑1 (MQ=255)
ggATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGaaa                                                                                                                                >  1:4233073/1‑140 (MQ=255)
ggATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGaaa                                                                                                                                >  1:4096805/1‑140 (MQ=255)
ggATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGaaa                                                                                                                                <  2:310487/140‑1 (MQ=255)
ggATATGGAGACATTTAAAAGATTAAAAACGTTGTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGaaa                                                                                                                                <  1:2631849/140‑1 (MQ=255)
    atGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGt                                                                                                                            <  2:1175607/140‑1 (MQ=255)
    atGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGt                                                                                                                            <  2:1991049/140‑1 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGTTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTAAGATTGATGAAATCGGaa                                                                                                                          >  2:2343571/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTTATTACTGCGATTACTGTCGTTTAACCCAATATACTGCAAAATAAGATTGATTAAATAGTaa                                                                                                                          >  1:2504409/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATGCTGCAAATTACGATTGATTAAATCGGaa                                                                                                                          >  1:2923104/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTTATGAAATCGTaa                                                                                                                          >  2:213188/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTaa                                                                                                                          >  1:40012/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTaa                                                                                                                          >  2:2973519/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTaa                                                                                                                          >  2:2927213/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTaa                                                                                                                          >  2:4020513/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTaa                                                                                                                          >  1:1739078/1‑140 (MQ=255)
      ggAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCCAATATACAGCAAATTACGATTGATGAAAAAGTaa                                                                                                                          >  1:1746403/1‑140 (MQ=255)
          tcATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATggg                                                                                                                      <  1:317254/139‑1 (MQ=255)
           cATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATAGTAATgggg                                                                                                                     >  2:2005398/1‑140 (MQ=255)
           cATTTAAAAGATTAAAAACGTGTTATTGGCCTTACAGAACAGTGTTTATGTGGACGCTTCTGGCCATGCTTTTGAAGACTTCCATTACTGTCGTTTAAACGAGAATACTGCAAAATAAAATTGATTAAAACGTAATtggg                                                                                                                     >  1:3970041/1‑140 (MQ=255)
            aTTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGa                                                                                                                    <  1:509764/140‑1 (MQ=255)
             tttAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGTTGAAATCGTAATGGGGAg                                                                                                                   >  2:3028462/1‑140 (MQ=255)
              ttAAAAGATTAACAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  1:2553496/140‑1 (MQ=255)
              ttAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  1:1538712/140‑1 (MQ=255)
              ttAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  2:1482112/140‑1 (MQ=255)
              ttAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  1:2755869/140‑1 (MQ=255)
              ttAAAAGATTAAAAACGTTTGATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCGTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  2:240560/140‑1 (MQ=255)
              ttAAAAGATTAAAAAAGTTTTATTGGCCGTACAGAACAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  1:2524870/140‑1 (MQ=255)
              ttAAAAGACTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAgg                                                                                                                  <  1:3514666/140‑1 (MQ=255)
                         aaaaCGTTTTATTGGCCGGACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCt                                                                                                       <  1:1863527/140‑1 (MQ=255)
                           aaCGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTg                                                                                                     <  1:911686/140‑1 (MQ=255)
                           aaCGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTg                                                                                                     <  1:3718099/140‑1 (MQ=255)
                               ttttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGCTTGCTGAATTCGGAATGGGGAGGCAGTATCAGCTTgcagc                                                                                                 >  1:1917222/1‑140 (MQ=255)
                               ttttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCGGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagc                                                                                                 <  2:1912565/140‑1 (MQ=255)
                               ttttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCGATTATCCTGCAAATTACGATTTATGAAATCGTAATGGGGAGGCCGTATCAGCTTgcagc                                                                                                 >  1:1137736/1‑140 (MQ=255)
                               ttttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCCATTATACTGCAAAATACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagc                                                                                                 >  1:725012/1‑140 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATTCTTTTGATGACTGCGATTACTGTCGTTTAACCCATTAACCAGCAAAATAAGATTTATGAAAAACTAAAGGGGAGGCAGTAACACCTTgcatca                                                                                                >  1:22243/1‑140 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagca                                                                                                <  2:1813630/140‑1 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagca                                                                                                <  2:2238921/140‑1 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagca                                                                                                <  1:4191623/140‑1 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagca                                                                                                <  1:3862969/140‑1 (MQ=255)
                                tttATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTgcagca                                                                                                <  2:3862845/140‑1 (MQ=255)
                                     tGGCCGTACAGAATAGTTTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTTCCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:3826946/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTCTCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:1854264/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:1718155/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:4025897/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:1360363/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:2923292/140‑1 (MQ=255)
                                     tGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTAGCCATGCTTTTGATGACTGCAATTACTGTCGTTTATCCGATTATCCTGCAAATTACGATTGATGAGATCGTACTGGGACGGCAGTATCAGCTTGCGGCGTGGGt                                                                                           <  2:671032/140‑1 (MQ=255)
                                     tGGCCGGACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:291580/140‑1 (MQ=255)
                                     gggCCGTACAGAAAACTGGTTCTGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGt                                                                                           <  1:587979/139‑1 (MQ=255)
                                       gCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATTAAATAGTAATGGGGAGGCAGTATCAGCTTTCAGCAAGGGtga                                                                                         >  1:3850320/1‑138 (MQ=255)
                                         cGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTTATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGc                                                                                       >  2:893291/1‑140 (MQ=255)
                                              aGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTggg                                                                                  <  1:2868849/140‑1 (MQ=255)
                                                aaaaGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGAt                                                                                <  1:3794972/140‑1 (MQ=255)
                                                   aGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTa                                                                             <  1:3298304/140‑1 (MQ=255)
                                                     tgtTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTGGTTTACCCGATTATCCTGCAAAATACGATTTATTAAATCGTAATGGGGAGGCCGTATCAGCTTTCAGCATGGGTCAGCTTGGGATTTAtt                                                                           >  1:1499679/1‑140 (MQ=255)
                                                     tgtTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATACTGCAAATTAAGATTGATGAAATCGTAATGGGGCGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTAtt                                                                           >  1:136570/1‑140 (MQ=255)
                                                       tttATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGTGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATtgt                                                                         >  2:1780826/1‑140 (MQ=255)
                                                       tttATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCGATTAACCTGCAAATTAAGATTTATGAAATGGTAATGGGGAGGCCGTATCATCGTGCAGCCTGGGTCAGCTTGGGCGTTATtgg                                                                         >  1:3347145/1‑139 (MQ=255)
                                                       tttATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCGAATATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGGATGGGTCAGGTTGGGCTTTATtgt                                                                         >  2:2589242/1‑140 (MQ=255)
                                                               gTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATgg                                                                 >  2:3805088/1‑140 (MQ=255)
                                                               gTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATACTTCAAAATACGATTTATGAAATAGTAATTGGGAGGCAGTATAAACTTGCAGCAAGGGTCAGCTTGGGATTTATTTTGGTAAttg                                                                 >  1:2341796/1‑138 (MQ=255)
                                                               gTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATACTGCAAATTACGATTTATTAAATCGTAATGGGGAGGCCGTATAAGCTTGCAGCATGGGTGAGCTTTGGATTTATTGTGGTAATgg                                                                 >  1:2843823/1‑140 (MQ=255)
                                                               gTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTAACCGATTATACTGCAAATTAAGATTTATTAAATAGTAATGGGGAGGCAGTATAAGCTTGCAGCATGGGTGAGCTTGGGATTTATTGTGGTAATgg                                                                 >  1:1888799/1‑140 (MQ=255)
                                                                 cGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATACTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATTGGTCAGCTTGGGATTTATTTTGGTAATTGcc                                                               >  2:3502665/1‑140 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATtgatga                                                                                                                                  >  2:53988/1‑60 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATgg                                                                                                                       >  1:3579863/1‑71 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATgg                                                                                                                       <  2:3579863/71‑1 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGCTGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGCTTTATTTTGGTAATTGCCCTGATATGAATCg                                                  >  1:2202268/1‑140 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCtt                                                                                     <  1:825856/105‑1 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTACTGGCCCTGAAAGGAATCg                                                  >  2:2743960/1‑140 (MQ=255)
                                                                              gCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCg                                                  >  1:2825745/1‑140 (MQ=255)
                                                                               cTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGc                                                 <  2:1739078/140‑1 (MQ=255)
                                                                               cTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGc                                                 <  1:3720259/140‑1 (MQ=255)
                                                                               cTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGc                                                 <  1:891624/140‑1 (MQ=255)
                                                                                   tgatgaCTGCGATTCCTGTCGTTTACCCGAGTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCCTGGGTCAGGTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACt                                             >  2:3179322/1‑140 (MQ=255)
                                                                                      tgaCTGCGATGACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAACTCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCAGAAAGGAATCGCCACtttt                                          <  1:1974747/140‑1 (MQ=255)
                                                                                       gaCTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACttttt                                         <  1:631277/140‑1 (MQ=255)
                                                                                       gaCTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTAGCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACttttt                                         <  1:540441/140‑1 (MQ=255)
                                                                                          tGCTATTACTGTCGTTTACCCGATTATGCTGCAAATTACGATTGATGAAAAAGGACAGGGGAGGCAGTATCATCTTGCAGAATGGGTCAGAGTGGGATTTATTGTGGTAATGGCCCTGAACCGAAGCGCCACTTTTTTtc                                      >  1:3259237/1‑140 (MQ=255)
                                                                                            cGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTTTTGTGGTAATGGCCCTGCAAGGACTCGCCACttttttgaat                                    >  1:3900597/1‑136 (MQ=255)
                                                                                            cGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcat                                    <  2:4191359/140‑1 (MQ=255)
                                                                                            cGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcat                                    <  1:2649437/140‑1 (MQ=255)
                                                                                            cGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcat                                    <  2:2049439/140‑1 (MQ=255)
                                                                                            cGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcat                                    <  2:3861785/140‑1 (MQ=255)
                                                                                              aTTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTTATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcatca                                  <  2:2341793/140‑1 (MQ=255)
                                                                                              aTTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTTATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTtcatca                                  <  2:1888799/140‑1 (MQ=255)
                                                                                                    gTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATAttt                            >  1:1095775/1‑140 (MQ=255)
                                                                                                    gTCGTTTAACCGATTATCCTGCAAAATACGATTGATGAAATCGTAATGGGGAGGAAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTAATAAATAttt                            >  2:4284631/1‑140 (MQ=255)
                                                                                                           aCCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGat                     <  1:3265184/140‑1 (MQ=255)
                                                                                                           aCCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGat                     <  1:4327818/140‑1 (MQ=255)
                                                                                                               gATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGt                 >  1:231264/1‑140 (MQ=255)
                                                                                                                 ttATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGttt               <  2:612527/140‑1 (MQ=255)
                                                                                                                       tGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATCTGTTTGGAATc         >  2:2954829/1‑140 (MQ=255)
                                                                                                                         cAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGTTTGGAATCaa       >  1:1105982/1‑140 (MQ=255)
                                                                                                                              tACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGACTCGCCACTTTTTTTCATCAATATTTAGGTGATATGTTTGGACTCAAATCCg  >  2:3406057/1‑140 (MQ=255)
                                                                                                                              tACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGTTTGGAATCAAATCCg  >  1:2060140/1‑140 (MQ=255)
                                                                                                                              tACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTAATCAATATTTAGGTGATATGTTTGGAATAAAATCCg  >  1:3401046/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GGATATGGAGACATTTAAAAGATTAAAAACGTTTTATTGGCCGTACAGAAAAGTGTTTATGTGGTCGCTTCTGGCCATGCTTTTGATGACTGCGATTACTGTCGTTTACCCGATTATCCTGCAAATTACGATTGATGAAATCGTAATGGGGAGGCAGTATCAGCTTGCAGCATGGGTCAGCTTGGGATTTATTGTGGTAATGGCCCTGAAAGGAATCGCCACTTTTTTTCATCAATATTTAGGTGATATGTTTGGAATCAAATCCG  >  NZ_CP034943/1463691‑1463956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: