Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,488,371	G→T	16.2%	S509* (TCA→TAA)	rnjA ←	ribonuclease J1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,488,371	0	G	T	16.2%	152.0 / 3.0	72	S509* (TCA→TAA)	rnjA	ribonuclease J1
Reads supporting (aligned to +/- strand):  ref base G (15/42);  new base T (13/2);  total (28/44)
Fisher's exact test for biased strand distribution p-value = 3.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATGCTGACAACCACAATGACAAGACCTTCTTC  >  NZ_CP034943/1488234‑1488510                                                                                                                                          |                                                                                                                                            agtCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTGGTTGCATGACGTTTTTTAGGTGGTTTgtt                                                                                                                                           >  1:3680901/3‑138 (MQ=255)   tCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGAt                                                                                                                                         <  2:1781451/140‑1 (MQ=255)   tCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGAt                                                                                                                                         <  2:3939426/140‑1 (MQ=255)   tCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGATTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGAt                                                                                                                                         <  2:1541967/140‑1 (MQ=255)      atgatgATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAg                                                                                                                                      <  1:1012677/140‑1 (MQ=255)      atgatgATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAg                                                                                                                                      <  1:1218281/140‑1 (MQ=255)      atgatgATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAg                                                                                                                                      <  1:1316609/140‑1 (MQ=255)      atgatgATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAg                                                                                                                                      <  1:2608488/140‑1 (MQ=255)            atCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTg                                                                                                                                >  2:3634484/1‑140 (MQ=255)            atCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTAGTTCCATCACATTTTGTATGTGGTTTTTGATAATCTCTTg                                                                                                                                >  2:2728601/1‑140 (MQ=255)            atCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTAGTTCAAACACATTTTGTATGTGGTTTTATATAAACTCttt                                                                                                                                >  2:2868902/1‑139 (MQ=255)              cGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCACGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTATGTGGTTTTTGATAAGATCtttcg                                                                                                                              >  2:2350046/1‑137 (MQ=255)              cGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAg                                                                                                                              <  1:872532/140‑1 (MQ=255)              cGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAg                                                                                                                              <  2:2169153/140‑1 (MQ=255)              cGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAg                                                                                                                              >  2:2837456/1‑140 (MQ=255)              cGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTGGGTGCCTCACTTTTTGTTGGTGGTTTTATATAAGCTCTTGAg                                                                                                                              >  2:1859482/1‑140 (MQ=255)                      tcatcGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTGGTTGCATCACTTTTTTTTGGTGGGTTTGGATAAACGATTGATCCTCAttt                                                                                                                      >  1:1343367/1‑139 (MQ=255)                      tcatcGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTAGTTGCCTCACGTTTTTTTTGTGGTTTTTGATTAACGCTTTAGCGTCAGTg                                                                                                                      >  1:1779802/1‑140 (MQ=255)                        atcGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCAGCACTTTTTGTAGGTGGTTTGTGATAAGCTCGTGTGCGtgattgat                                                                                                                    >  1:4135037/1‑140 (MQ=255)                        atcGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTCGTTCCATCACTTTTTGTAGGTGGTTTTATATTATCTGTTGTGAGTCATTGAt                                                                                                                    >  2:3236049/1‑140 (MQ=255)                         tcGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATc                                                                                                                   <  2:2726704/140‑1 (MQ=255)                               ggCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCATACCATTGAGTCGTTTTTAGTTACATCACTTTTTTTAGGTGGTTTGATATAAGCTCTTGAGCGTAATTGATAAATTCg                                                                                                             >  2:2022124/1‑140 (MQ=255)                               ggCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTAGTTACAACACTTTTTGTAGGTTGTTTGAGATAAGCACTTGATCGTAATTGATAAATTCg                                                                                                             >  2:2182402/1‑140 (MQ=255)                                   tttcgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAg                                                                                                         <  1:2350046/140‑1 (MQ=255)                                   tttcgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAg                                                                                                         <  1:2837456/140‑1 (MQ=255)                                    ttcgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTCGTTCCATCACTTTTTGTAGGTGGTTTTATATAAGCTCTTGAGCGTAATTGATCAAGTCGCCAGa                                                                                                        >  2:4150645/1‑140 (MQ=255)                                      cgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATTAGCTCTTGAGCGTCTTTGATCAAGTCGCCAGAtt                                                                                                      <  1:2853750/140‑1 (MQ=255)                                      cgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGAtt                                                                                                      <  1:1013336/140‑1 (MQ=255)                                      cgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGAtt                                                                                                      <  1:2929368/140‑1 (MQ=255)                                      cgtttTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGAtt                                                                                                      <  1:2855404/140‑1 (MQ=255)                                      cgatttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGAtt                                                                                                      <  1:3495004/137‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  2:2679634/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  2:3140115/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  2:1099944/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  1:1174421/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  1:1280636/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  1:3797031/140‑1 (MQ=255)                                        ttttttCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTGTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTGTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATtct                                                                                                    <  2:950231/140‑1 (MQ=255)                                        ttttttAATTTAAGAACGGCGCAAGCGTGTTTGTAATCTCATTTTTTATTTCTGACCATTTAGTCGTTTTTCTTTCAATCACTTTTTGTAGTTGGTTTTAGATAAGGTCTTGAGCTTCATTTATCTATTCGACAGATtct                                                                                                    <  2:1467545/140‑1 (MQ=255)                                              cATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATg                                                                                              <  2:458232/140‑1 (MQ=255)                                              cATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATg                                                                                              <  1:2793606/140‑1 (MQ=255)                                                   gagacaggCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTacac                                                                                         <  1:454825/134‑1 (MQ=255)                                                           cgcAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACATTTTGTATGTGGTTTTATATAAAAACTTGATCGTCATTGAAAAATTCGCCACATTATGTCATGTACAaaaaaacaa                                                                                 >  1:2987229/1‑135 (MQ=255)                                                           cgcAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTAGTTCCATCAAGTTTTTTAGGTGGTTTGAGATAATCTCGTGGGCGTCAATGATCAAGTCGCCAGATTATCTCATGTACCCaaaaacac                                                                                 >  2:2033459/1‑135 (MQ=255)                                                           cgcAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTCGTTCCAACCCGGTGTGTTGGTGGTTTGTGGTAATCTGGTGGGGGTGATTGTTCAAGTCGCCCGGTGGGGTCATGTTCACaaaacggc                                                                                 >  1:1008823/1‑135 (MQ=255)                                                           cgcAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTAGTTGCCTAACTTTTTTTAGGTGGTTTTTGATAAACTATTTAGCGTCATTGATGAAATCGCCCCAGTGTCTCATGTAAACaaaaacta                                                                                 >  1:2381472/1‑135 (MQ=255)                                                                gCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTGCATAACTTTTTTTAGGTGGTTTGTGATAAGCTGTTTATCGTGATTGTTCAAGTCGCCAGATTATCTCATGTACACAAAAACTATGGaa                                                                            >  2:3584686/1‑140 (MQ=255)                                                                gCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACGTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCAAGTACACAAAACCTCTGGaa                                                                            >  2:4293887/1‑140 (MQ=255)                                                                gCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACATTTTGTAGGTGGTTTTAGCTAAGCTCGTGAGCGTCATTGATCAAGTCGCCACATTATCTCATGTACCAAAAACCACTggta                                                                            >  2:2705245/1‑138 (MQ=255)                                                                gCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTACATCACGTTTTGTAGGTGGTTTGAGATAATCTCTTTAGCGTCATTGATAAAGTCGCCAGATTCTCTCAAGTACACAAATACTCTGGaa                                                                            >  2:3489345/1‑140 (MQ=255)                                                                gCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTAGGTCCATCCCGTTTTGTATGTGGGTTTAGATAAGCTCGTGAGCGTCATTGATAAAGTCGGCAGATTGTCTCATGTACACAAATACTCTGGaa                                                                            >  2:3247036/1‑140 (MQ=255)                                                                  gtgtCTGTAATCTGATTTTTGATTTCAGACCATTGAGTCGATTTTCGTTCCATCACTTTTTTGAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGaaat                                                                          <  1:170255/140‑1 (MQ=255)                                                                  gtgtCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGaaat                                                                          <  1:3440192/140‑1 (MQ=255)                                                                  gtgtCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGaaat                                                                          <  1:492013/140‑1 (MQ=255)                                                                  gtgtCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGaaat                                                                          <  1:422334/140‑1 (MQ=255)                                                                  gtgtCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGaaat                                                                          <  1:3697948/140‑1 (MQ=255)                                                                     tCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTGGTTGCCTGACGTTTTTTATGTGGTTTGTGATTAGCTGGTTTGCGGGATTGTTCAAATAGCCAGAGTATCTCATGTACACAAATAATCTGGaaataaa                                                                       >  1:3683595/1‑140 (MQ=255)                                                                     tCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTGTTTCGTTACATAACTTTTTGTAGGTGGTTTGTGATAAGCTCTGTCGCGGCCTTGTTGAACTCGCCAGTTTCTCTACTGTTAACTAATCCTCTGGaaaacaa                                                                       >  2:2324909/1‑140 (MQ=255)                                                                               cATTTTTGATTTCAGACCATTGAGTCGTTTTTAGTTCCATCACTTTTTTTATGTGGTTTGAGATAAGCTCTTTAGCGTCATTTATAAAGTCGCCAGATTATATCATGTACACAAATACTCTGGAAATCAAATAAGGTccc                                                             >  2:2246056/1‑140 (MQ=255)                                                                                    ttGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGGCCCGCTGa                                                        <  2:2270090/140‑1 (MQ=255)                                                                                            aGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTTAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTaa                                                <  2:3275611/140‑1 (MQ=255)                                                                                            aGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTaa                                                <  1:40370/140‑1 (MQ=255)                                                                                            aGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTaa                                                <  1:3113980/140‑1 (MQ=255)                                                                                              aCCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAg                                              <  1:1796234/140‑1 (MQ=255)                                                                                              aCCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAg                                              <  1:2246056/140‑1 (MQ=255)                                                                                                          tttttGGTTCCATAACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAATTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAAAAAAATAAGGTCCCGCTGAAATCTTAAATACTTCCATTTCa                                  >  2:4241960/1‑140 (MQ=255)                                                                                                          tttttCGTTCCATCACTTTTTGTAGGTTGTTTGATATAAGCTCTTGAGCGTCATTGCTCAAGTAGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCa                                  <  2:3741830/140‑1 (MQ=255)                                                                                                          tttttCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCa                                  <  2:1779802/140‑1 (MQ=255)                                                                                                          tttttCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCa                                  <  1:3634484/140‑1 (MQ=255)                                                                                                             ttCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATg                               <  2:1249527/140‑1 (MQ=255)                                                                                                                 ttCCATCACTTTTTGTAGGTGGTTTTTGATAAGCTATTTAGCGTCAATTATCAAGTCGCCACATTCACTCAAGTACACAAAACCTCTGGAAAACAAAAAAGGTCCCGCTGAAATAATAAAACCATCCAAGTAAATGcata                           >  1:3699234/1‑137 (MQ=255)                                                                                                                  gccATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATGCTGAc                          <  2:1334497/139‑1 (MQ=255)                                                                                                                                    tggtTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATGCTGACAACCACAATGACAAGAcc        >  1:1173553/1‑140 (MQ=255)                                                                                                                                          gagaTAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCCATGCTGACAACCACAATGACAAGACCttcttc  <  2:3470636/140‑1 (MQ=255)                                                                                                                                          gagaTAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATGCTGACAACCACAATGACAAGACCttcttc  <  2:3683595/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            CTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAGCGTGTCTGTAATCTCATTTTTGATTTCAGACCATTGAGTCGTTTTTCGTTCCATCACTTTTTGTAGGTGGTTTGAGATAAGCTCTTGAGCGTCATTGATCAAGTCGCCAGATTCTCTCATGTACACAAATCCTCTGGAAATCAAATCAGGTCCCGCTGAAATCTTAAAGCCTTCCATGTCAATGCTGACAACCACAATGACAAGACCTTCTTC  >  NZ_CP034943/1488234‑1488510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑